scholarly journals Intermittent PTH administration improves alveolar bone formation in type 1 diabetic rats with periodontitis

2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Ji-Hye Kim ◽  
Ae Ri Kim ◽  
Yun Hui Choi ◽  
Aeryun Kim ◽  
Yongsung Sohn ◽  
...  
Keyword(s):  
Bone Formation ◽  
Alveolar Bone ◽  
Diabetic Rats ◽  
Intermittent Pth

scholarly journals Diabetes Enhances Periodontal Bone Loss through Enhanced Resorption and Diminished Bone Formation

2006 ◽  
Vol 85 (6) ◽  
pp. 510-514 ◽  
Author(s):  
R. Liu ◽  
H.S. Bal ◽  
T. Desta ◽  
N. Krothapalli ◽  
M. Alyassi ◽  
...  
Keyword(s):  
Bone Loss ◽  
Bone Formation ◽  
Alveolar Bone ◽  
Diabetic Rats ◽  
New Bone Formation ◽  
Periodontal Ligament Fibroblasts ◽  
Zdf Rat ◽  
Pdl Cells ◽  
Periodontal Bone Loss

Using a ligature-induced model in type-2 Zucker diabetic fatty (ZDF) rat and normoglycemic littermates, we investigated whether diabetes primarily affects periodontitis by enhancing bone loss or by limiting osseous repair. Diabetes increased the intensity and duration of the inflammatory infiltrate (P < 0.05). The formation of osteoclasts and percent eroded bone after 7 days of ligature placement was similar, while four days after removal of ligatures, the type 2 diabetic group had significantly higher osteoclast numbers and activity (P < 0.05). The amount of new bone formation following resorption was 2.4- to 2.9-fold higher in normoglycemic vs. diabetic rats (P < 0.05). Diabetes also increased apoptosis and decreased the number of bone-lining cells, osteoblasts, and periodontal ligament fibroblasts (P < 0.05). Thus, diabetes caused a more persistent inflammatory response, greater loss of attachment and more alveolar bone resorption, and impaired new bone formation. The latter may be affected by increased apoptosis of bone-lining and PDL cells.

scholarly journals Different Stages of Alveolar Bone Repair Process Are Compromised in the Type 2 Diabetes Condition: An Experimental Study in Rats

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 471
Author(s):  
Letícia Pitol-Palin ◽  
Fábio Roberto de Souza Batista ◽  
Pedro Henrique Silva Gomes-Ferreira ◽  
Gabriel Mulinari-Santos ◽  
Edilson Ervolino ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Bone Formation ◽  
Bone Remodeling ◽  
Bone Repair ◽  
Alveolar Bone ◽  
Repair Process ◽  
Diabetic Rats ◽  
Collagen Fibers ◽  
Volume Percentage

The aim of this study was to analyze the stages of the alveolar bone repair in type 2 diabetic rats evaluating the mechanism of mineralization and bone remodeling processes after dental extraction. Forty-eight rats were divided into normoglycemic (NG) and type 2 diabetes (T2D) groups. The upper right incisor was extracted and after 3, 7, 14 and 42 days the animals were euthanized. The following analyses were performed: immunolabeling against antibodies TNFα, TGFβ, IL6, WNT, OCN and TRAP, collagen fibers maturation, microtomography and confocal microscopy. Data were submitted to statistical analysis. The immunolabeling analysis showed that the T2D presented a more pronounced alveolar inflammation than NG. Labeling of proteins responsible for bone formation and mineralization was higher in NG than T2D, which presented greater resorptive activity characterized by TRAP labeling. Also, T2D group showed a decrease in the amount of collagen fibers. Micro-CT analysis showed that T2D causes a decrease in bone volume percentage due to deficient trabecular parameters and higher porosity. The T2D bone dynamics show a loss in bone remodeling process. T2D prolongs the local inflammatory process, which impairs the organization and maturation of collagen fibers, delaying bone formation that generates impact on mineralization and bone turnover.

scholarly journals Green Tea Modulates Cytokine Expression in the Periodontium and Attenuates Alveolar Bone Resorption in Type 1 Diabetic Rats

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0134784 ◽  
Author(s):  
Gabriela Gennaro ◽  
Marcela Claudino ◽  
Tania Mary Cestari ◽  
Daniele Ceolin ◽  
Patrícia Germino ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Green Tea ◽  
Alveolar Bone ◽  
Diabetic Rats ◽  
Cytokine Expression

Parathyroid Hormone Protects against Periodontitis-associated Bone Loss

2003 ◽  
Vol 82 (10) ◽  
pp. 791-795 ◽  
Author(s):  
S.P. Barros ◽  
M.A.D. Silva ◽  
M.J. Somerman ◽  
F.H. Nociti
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Bone Loss ◽  
Bone Formation ◽  
Morphometric Analysis ◽  
Alveolar Bone ◽  
Inflammatory Cells ◽  
Alveolar Bone Loss ◽  
Intermittent Pth ◽  
Major Mediator

Parathyroid hormone (PTH) functions as a major mediator of bone remodeling and as an essential regulator of calcium homeostasis. In addition to the well-established catabolic effects (activation of bone resorption) of PTH, it is now recognized that intermittent PTH administration has anabolic effects (promotion of bone formation). The aim of this study was to investigate whether intermittent administration of PTH in rodents would block the alveolar bone loss observed in rats when a ligature model of periodontitis is used. Morphometric analysis showed that intermittent PTH administration (40 μg/kg) was able to protect the tooth site from periodontitis-induced bone resorption. In addition, there was a significant reduction in the number of inflammatory cells at the marginal gingival area in sections obtained from animals receiving PTH compared with control animals. These findings demonstrated that intermittent PTH administration was able to protect against periodontitis-associated bone loss in a rodent model.

Parathyroid hormone-related peptide (1–34) reduces alveolar bone loss in type 1 diabetic rats

2017 ◽  
Vol 83 ◽  
pp. 13-19 ◽  
Author(s):  
Wen Zhang ◽  
Su-Zhen Wu ◽  
Jian Zhou ◽  
Hong-Min Chen ◽  
Ye-Li Gong ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Loss ◽  
Alveolar Bone ◽  
Diabetic Rats ◽  
Alveolar Bone Loss ◽  
Related Peptide ◽  
Parathyroid Hormone Related Peptide

scholarly journals Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats

Cell Reports ◽  
2020 ◽  
Vol 32 (13) ◽  
pp. 108207
Author(s):  
Xiao Wei ◽  
Zongshi Lu ◽  
Li Li ◽  
Hexuan Zhang ◽  
Fang Sun ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Diabetic Rats ◽  
Ampk Activity

scholarly journals In Vivo Study for Clinical Application of Dental Stem Cell Therapy Incorporated with Dental Titanium Implants

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 381
Author(s):  
Hyunmin Choi ◽  
Kyu-Hyung Park ◽  
Narae Jung ◽  
June-Sung Shim ◽  
Hong-Seok Moon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Alveolar Bone ◽  
Human Mesenchymal Stem Cells ◽  
Titanium Implants ◽  
Osteogenic Potential ◽  
Induction Medium ◽  
Radiographic Analysis ◽  
New Bone Formation

The aim of this study was to investigate the behavior of dental-derived human mesenchymal stem cells (d-hMSCs) in response to differently surface-treated implants and to evaluate the effect of d-hMSCs on local osteogenesis around an implant in vivo. d-hMSCs derived from alveolar bone were established and cultured on machined, sandblasted and acid-etched (SLA)-treated titanium discs with and without osteogenic induction medium. Their morphological and osteogenic potential was assessed by scanning electron microscopy (SEM) and real-time polymerase chain reaction (RT-PCR) via mixing of 5 × 106 of d-hMSCs with 1 mL of Metrigel and 20 μL of gel-cell mixture, which was dispensed into the defect followed by the placement of customized mini-implants (machined, SLA-treated implants) in New Zealand white rabbits. Following healing periods of 2 weeks and 12 weeks, the obtained samples in each group were analyzed radiographically, histomorphometrically and immunohistochemically. The quantitative change in osteogenic differentiation of d-hMSCs was identified according to the type of surface treatment. Radiographic analysis revealed that an increase in new bone formation was statistically significant in the d-hMSCs group. Histomorphometric analysis was in accordance with radiographic analysis, showing the significantly increased new bone formation in the d-hMSCs group regardless of time of sacrifice. Human nuclei A was identified near the area where d-hMSCs were implanted but the level of expression was found to be decreased as time passed. Within the limitations of the present study, in this animal model, the transplantation of d-hMSCs enhanced the new bone formation around an implant and the survival and function of the stem cells was experimentally proven up to 12 weeks post-sacrifice.

scholarly journals Cyclodextrin-Based Supramolecular Complexes of Osteoinductive Agents for Dental Tissue Regeneration

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 136
Author(s):  
Masahiko Terauchi ◽  
Atsushi Tamura ◽  
Yoshinori Arisaka ◽  
Hiroki Masuda ◽  
Tetsuya Yoda ◽  
...  
Keyword(s):  
Growth Factors ◽  
Bone Formation ◽  
Small Molecule ◽  
Tissue Regeneration ◽  
Alveolar Bone ◽  
Inclusion Complexation ◽  
Dental Tissue ◽  
Polyelectrolyte Complexes ◽  
Small Molecule Drugs

Oral tissue regeneration has received growing attention for improving the quality of life of patients. Regeneration of oral tissues such as alveolar bone and widely defected bone has been extensively investigated, including regenerative treatment of oral tissues using therapeutic cells and growth factors. Additionally, small-molecule drugs that promote bone formation have been identified and tested as new regenerative treatment. However, treatments need to progress to realize successful regeneration of oral functions. In this review, we describe recent progress in development of regenerative treatment of oral tissues. In particular, we focus on cyclodextrin (CD)-based pharmaceutics and polyelectrolyte complexation of growth factors to enhance their solubility, stability, and bioactivity. CDs can encapsulate hydrophobic small-molecule drugs into their cavities, resulting in inclusion complexes. The inclusion complexation of osteoinductive small-molecule drugs improves solubility of the drugs in aqueous solutions and increases in vitro osteogenic differentiation efficiency. Additionally, various anionic polymers such as heparin and its mimetic polymers have been developed to improve stability and bioactivity of growth factors. These polymers protect growth factors from deactivation and degradation by complex formation through electrostatic interaction, leading to potentiation of bone formation ability. These approaches using an inclusion complex and polyelectrolyte complexes have great potential in the regeneration of oral tissues.

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