scholarly journals High intensity exercise downregulates FTO mRNA expression during the early stages of recovery in young males and females

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Jessica Danaher ◽  
Christos G. Stathis ◽  
Robin A. Wilson ◽  
Alba Moreno-Asso ◽  
R. Mark Wellard ◽  
...  
Keyword(s):  
Mrna Expression ◽  
High Intensity ◽  
High Intensity Exercise ◽  
Young Males ◽  
Early Stages ◽  
Males And Females

scholarly journals High intensity exercise downregulates FTO mRNA expression during the early stages of recovery in young males and females

2020 ◽  
Author(s):  
Jessica Danaher ◽  
Christos G. Stathis ◽  
Robin A. Wilson ◽  
Alba Moreno-Asso ◽  
R. Mark Wellard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Expression ◽  
Mrna Expression ◽  
High Intensity ◽  
Vastus Lateralis ◽  
Preliminary Evidence ◽  
High Intensity Exercise ◽  
Nutritional Regulation ◽  
Ergometer Exercise ◽  
Males And Females

Abstract Background Physical exercise and activity status may modify the effect of fat mass- and obesity-associated (FTO) genotype on body weight and obesity risk. To understand the interaction between FTO’s effect and physical activity, the present study investigated the effects of high and low intensity exercise on FTO mRNA and protein expression, and potential modifiers of exercise-induced changes in FTO in healthy-weighted individuals. Methods Twenty-eight untrained males and females (25.4 ± 1.1 years; 73.1 ± 2.0 kg; 178.8 ± 1.4 cm; 39.0 ± 1.2 ml.kg.min -1 VO 2peak ) were genotyped for the FTO rs9939609 (T>A) polymorphism and performed isocaloric (400 kcal) cycle ergometer exercise on two separate occasions at different intensities: 80% (HI) and 40% (LO) VO 2peak . Skeletal muscle biopsies ( vastus lateralis ) and blood samples were taken pre-exercise and following 10 and 90 mins passive recovery.Results FTO mRNA expression was significantly decreased after HI intensity exercise ( p =0.003). No differences in basal and post-exercise FTO protein expression were evident between FTO genotypes. Phosphorylated AMPK and AS160 were significantly increased following HI intensity exercise ( p <0.05). Multivariate models of metabolomic data (O2PLS-DA) were unable to detect any significant metabolic differences between genotypes with either exercise trial ( p >0.05). However, skeletal muscle glucose accumulation at 10 mins following HI ( p =0.021) and LO ( p =0.033) intensity exercise was greater in AA genotypes compared to TT genotypes. Conclusion Our novel data provides preliminary evidence regarding the effects of exercise on FTO expression in skeletal muscle. Specifically, high intensity exercise downregulates expression of FTO mRNA and suggests that in addition to nutritional regulation, FTO could also be regulated by exercise.

scholarly journals High intensity exercise downregulates FTO mRNA expression during the early stages of recovery in young males and females

2020 ◽  
Author(s):  
Jessica Danaher ◽  
Christos G. Stathis ◽  
Robin A. Wilson ◽  
Alba Moreno-Asso ◽  
R. Mark Wellard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Expression ◽  
Mrna Expression ◽  
High Intensity ◽  
Vastus Lateralis ◽  
High Intensity Exercise ◽  
Nutritional Regulation ◽  
Low Intensity ◽  
Ergometer Exercise ◽  
Males And Females

Abstract Background: Physical exercise and activity status may modify the effect of fat mass- and obesity-associated (FTO) genotype on body weight and obesity risk. To understand the interaction between FTO’s effect and physical activity, the present study investigated the effects of high and low intensity exercise on FTO mRNA and protein expression, and potential modifiers of exercise-induced changes in FTO in healthy-weighted individuals. Methods: Twenty-eight untrained males and females (25.4 ± 1.1 years; 73.1 ± 2.0 kg; 178.8 ± 1.4 cm; 39.0 ± 1.2 ml.kg.min-1 VO2peak) were genotyped for the FTO rs9939609 (T>A) polymorphism and performed isocaloric (400 kcal) cycle ergometer exercise on two separate occasions at different intensities: 80% (High Intensity (HI)) and 40% (Low Intensity (LO)) VO2peak. Skeletal muscle biopsies (vastus lateralis) and blood samples were taken pre-exercise and following 10 and 90 mins passive recovery. Results: FTO mRNA expression was significantly decreased after HI intensity exercise (p=0.003). No differences in basal and post-exercise FTO protein expression were evident between FTO genotypes. Phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and Akt substrate of 160 kDa (AS160) were significantly increased following HI intensity exercise (p<0.05). Multivariate models of metabolomic data (orthogonal two partial least squares discriminant analysis (O2PLS-DA)) were unable to detect any significant metabolic differences between genotypes with either exercise trial (p>0.05). However, skeletal muscle glucose accumulation at 10 mins following HI (p=0.021) and LO (p=0.033) intensity exercise was greater in AA genotypes compared to TT genotypes.Conclusion: Our novel data provides preliminary evidence regarding the effects of exercise on FTO expression in skeletal muscle. Specifically, high intensity exercise downregulates expression of FTO mRNA and suggests that in addition to nutritional regulation, FTO could also be regulated by exercise. Trial Registration: ACTRN12612001230842. Registered 21 November 2012 – Retrospectively registered, https://www.anzctr.org.au/

Pre-exercise β-hydroxy-β-methylbutyrate free-acid supplementation improves work capacity recovery: a randomized, double-blinded, placebo-controlled study

2018 ◽  
Vol 43 (7) ◽  
pp. 691-696 ◽  
Author(s):  
Ana Luiza Matias Correia ◽  
Filipe Dinato de Lima ◽  
Martim Bottaro ◽  
Amilton Vieira ◽  
Andrew Correa da Fonseca ◽  
...  
Keyword(s):  
Single Dose ◽  
High Intensity ◽  
Time Course ◽  
Free Acid ◽  
Exercise Bout ◽  
Work Capacity ◽  
High Intensity Exercise ◽  
Controlled Study ◽  
Exercise Protocol ◽  
Young Males

The purpose of this study was to investigate the effects of a single-dose of β-hydroxy-β-methylbutyrate free acid (HMB-FA) supplementation on muscle recovery after a high-intensity exercise bout. Twenty-three trained young males were randomly assigned to receive either a single-dose supplementation of 3 g of HMB-FA (n = 12; age, 22.8 ± 3.0 years) or placebo (PLA; n = 11; age, 22.9 ± 3.1 years). A muscle damage protocol was applied 60 min after supplementation, and consisted of 7 sets of 20 drop jumps from a 60-cm box with 2-min rest intervals between sets. Muscle swelling, countermovement jump (CMJ), maximal voluntary isometric torque (MVIT), and work capacity (WC) were measured before, immediately after, and 24, 48, and 72 h after the exercise protocol. Muscle swelling, CMJ, and MVIT changed similarly in both groups after the exercise protocol (p < 0.001), but returned to pre-exercise levels after 24 h in both groups. WC decreased similarly in both groups after the exercise protocol (p < 0.01). For HMB-FA, WC returned to pre-exercise level 24 h after exercise protocol. However, for PLA, WC did not return to pre-exercise level even 72 h after the exercise protocol. In summary, a single-dose of HMB-FA supplementation improved WC recovery after a high-intensity exercise bout. However, HMB-FA did not affect the time-course of muscle swelling, MVIT, and CMJ recovery.

EFFECT OF TWO DIFFERENT PERCEIVED EXERTION SCALES ON HIGH INTENSITY EXERCISE ENDURANCE IN MALES AND FEMALES

1980 ◽  
Vol 21 (Supplement) ◽  
pp. S5
Author(s):  
S. F. Loy ◽  
C. Compton ◽  
J. Hayes ◽  
M. Carlstedt ◽  
A. Stutts ◽  
...  
Keyword(s):  
High Intensity ◽  
Perceived Exertion ◽  
High Intensity Exercise ◽  
Males And Females ◽  
Exercise Endurance

EFFECTS OF CARBOHYDRATE FEEDINGS ON FATIGUE DURING INTERMITTENT HIGH-INTENSITY EXERCISE IN MALES AND FEMALES

1995 ◽  
Vol 27 (Supplement) ◽  
pp. S223 ◽  
Author(s):  
D. A. Jackson ◽  
J. M. Davis ◽  
M. S. Broadwell ◽  
J. L. Query ◽  
C. L. Lambert
Keyword(s):  
High Intensity ◽  
High Intensity Exercise ◽  
Males And Females

Effects of Jump Training on Procollagen α1(I) mRNA Expression and Its Relationship

2004 ◽  
Vol 29 (2) ◽  
pp. 157-171 ◽  
Author(s):  
With Collagen Concentration
Keyword(s):  
Mrna Expression ◽  
High Intensity ◽  
Collagen Synthesis ◽  
Mrna Level ◽  
High Intensity Exercise ◽  
Main Role ◽  
Mrna Levels ◽  
Collagen Concentration ◽  
Jump Training ◽  
Psoas Muscles

The aim of this study was to examine the effects of a prolonged high-intensity exercise, jumping, on procollagen α1(I) mRNA level and collagen concentration in different muscles of trained (T) and control (C) rabbits. Procollagen α1 (I) mRNA expression was much higher (2.8 to 23.5 times) in semimembranosus proprius (SMP), a slow-twitch oxidative muscle, than in extensor digitorum longus (EDL), rectus femoris (RF), and psoas major (Psoas) muscles, both fast-twitch mixed and glycolytic, whatever group was considered (p <  0.001). Procollagen α1(I) mRNA level also decreased significantly between 50 and 140 days in all muscles (0.001 <  p <  0.01). However, mRNA levels were 16 to 97% greater at 140 days in all muscles of T animals compared to C ones (0.01 <  p < 0.05). Collagen concentrations of EDL and RF muscles were also higher (14 to 19%) in T than in C rabbits at 90 and 140 days (0.001 <  p <  0.05). In the whole sample, collagen concentration was negatively associated with the procollagen α1(I) mRNA level in EDL and RF muscles (−0.49 <  r <  −0.44, p <  0.05), while being positively related to mRNA expression in SMP and Psoas muscles (0.65 <  r <  0.85, p <  0.01). It is concluded that jump training clearly restricts the decrease of procollagen (I) mRNA level and probably affects collagen synthesis level. In trained rabbit muscles, the maintenance of a better synthesis level could partly explain the higher collagen concentrations found in EDL and RF at 140 days. Nevertheless, the collagen degradation process seems to play the main role in the increase of total collagen concentration with age in EDL and RF muscles. Key words: collagen synthesis, high intensity exercise, muscle type, age

scholarly journals EFFECT OF TWO DIFFERENT PERCEIVED EXERTION SCALES ON HIGH INTENSITY EXERCISE ENDURANCE IN MALES AND FEMALES

1989 ◽  
Vol 21 (Supplement) ◽  
pp. S5
Author(s):  
S. F. Loy ◽  
C. Compton ◽  
J. Hayes ◽  
M. Carlstedt ◽  
A. Stutts ◽  
...  
Keyword(s):  
High Intensity ◽  
Perceived Exertion ◽  
High Intensity Exercise ◽  
Males And Females ◽  
Exercise Endurance

The Effects of Acute High-Intensity Exercise on Circulating Endothelial Microparticles in Overweight/obese Males and Females

2014 ◽  
Vol 46 ◽  
pp. 712
Author(s):  
Cody G. Durrer ◽  
Nic Martinez ◽  
Michelle L. Hummel ◽  
Nathan T. Jenkins ◽  
Marcus W. Kilpatrick ◽  
...  
Keyword(s):  
High Intensity ◽  
High Intensity Exercise ◽  
Endothelial Microparticles ◽  
Males And Females

scholarly journals One-month of high-intensity exercise did not change the food intake and the hypothalamic arcuate nucleus proopiomelanocortin and neuropeptide Y expression levels in male Wistar rats

2019 ◽  
Vol 53 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Nazli Khajehnasiri ◽  
Homayoun Khazali ◽  
Farzam Sheikhzadeh ◽  
Mahnaz Ghowsi
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Mrna Expression ◽  
High Intensity ◽  
Wistar Rats ◽  
Arcuate Nucleus ◽  
The Body ◽  
High Intensity Exercise ◽  
Hypothalamic Arcuate Nucleus ◽  
Male Wistar Rats

AbstractObjective. The hypothalamic arcuate nucleus proopiomelanocortin (POMC) and neuropeptide Y (NPY) circuitries are involved in the inhibition and stimulation of the appetite, respectively. The aim of this study was to investigate the effects of one-month lasting high-intensity exercise on the POMC mRNA and NPY mRNA expression in the above-mentioned brain structure and appetite and food intake levels.Methods. Fourteen male Wistar rats (250±50 g) were used and kept in the well-controlled conditions (22±2 °C, 50±5% humidity, and 12 h dark/light cycle) with food and water ad libitum. The rats were divided into two groups (n=7): 1) control group (C, these rats served as controls) and 2) exercised group (RIE, these rats performed a high-intensity exercise for one month (5 days per week) 40 min daily with speed 35 m/min. The total exercise time was 60 min. The body weight and food intake were recorded continuously during the experiments.Results. The results showed relative mRNA expression of POMC and NPY estimated in the hypothalamic arcuate nucleus. There were no significant differences in the NPY and POMC mRNAs expression levels and food intake between C and RIE groups.Conclusions. The present data indicate that one-month regular intensive exercise did not alter the levels of NPY and POMC mRNAs expression (as two important factors in the regulation of appetite) in the hypothalamic arcuate nucleus and food intake suggesting that this type of exercise itself is not an appropriate procedure for the body weight reduction.

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