A simple and fast Agrobacterium-mediated transformation system for passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa)

Abstract Background Passion fruit (Passiflora edulis Sims) is an important horticultural crop in the tropics and subtropics, where it has great commercial potential due to high demand for fresh edible fruits and processed juice as well as source of raw materials in cosmetic industries. Genetic engineering shows great potential in passion fruit improvement and can compensate for the limitations of conventional breeding. Despite the success achieved in genetic modification of few passion fruit varieties, transgenic passion fruit production is still difficult for farmer-preferred cultivars. Therefore, it is important to establish a simple and fast Agrobacterium-mediated cell transformation of commercial hybrid passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa). Results In the present study, we have developed a simple and fast Agrobacterium-mediated transformation system for hybrid passion fruit KPF4 using leaf disc explants. Factors affecting the rate of transient beta (β)-glucuronidase (gusA) expression and consequently transformation efficiency were optimized as follows: Agrobacterium cell density with an OD600 of 0.5, 30 min infection time, 3 days of co-cultivation duration and the incorporation of 200 µM acetosyringone into Agrobacterium infection suspension medium. Using the optimized conditions, transgenic plants of KPF4 were produced within 2 months with an average transformation efficiency of 0.67%. The β-glucuronidase (GUS) histochemical staining confirmed the expression and integration of an intron-containing gusA gene into transformed leaf discs and transgenic plant lines of KPF4. The presence of gusA gene in the transgenic plants was confirmed by polymerase chain reaction (PCR). The results confirmed that the gusA gene was efficiently integrated into the passion fruit genome. Conclusions The developed transformation protocol is simple and rapid and could be useful for functional genomic studies and transferring agronomically important traits into passion fruit hybrid KPF4. This study developed a method that can be used to transfer traits such as resistance to viral diseases, low fruit quality and short storage life. To the best of our knowledge, this is the first report on genetic transformation system for commercial passion fruit hybrid KPF4.

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Visitation of bees to flowers of yellow passion fruit in Northern Paraná State, Brazil

Agronomy Science and Biotechnology ◽

10.33158/asb.2017v3i1p12 ◽

2017 ◽

Vol 3 (1) ◽

pp. 12

Author(s):

Humberto Godoy Androcioli ◽

Ayres de Oliveira Menezes Junior ◽

Ana Odete Santos Vieira ◽

Dayanne Fabricio Bressan ◽

Adriano Thibes Hoshino ◽

...

Keyword(s):

High Frequency ◽

Negative Impact ◽

Low Frequency ◽

Fruit Production ◽

Passion Fruit ◽

Agricultural Expansion ◽

Passiflora Edulis ◽

Floral Visitors ◽

Yellow Passion Fruit ◽

Fruit Plantations

The yellow passion fruit (Passiflora edulis f. flavicarpa) needs pollinators for fructification due to the existence of mechanisms that impede self-pollinating. Pollinators of great efficiency for the yellow passion fruit in Brazil are the bumblebee species (Xylocopa frontalis, X. grisescens and X. Suspecta). Deforestation by agricultural expansion in the region has a negative impact on these pollinators’ populations, leaving space for the action of cleptobiotic species with further damage to fruit production. The objective of this study was to evaluate the frequency of pollinating bees and floral visitors during yellow passion fruit culture’s post anthesis period in Northern Paraná and its fructification effects. Three passion fruit plantations in Northern Paraná were evaluated in the counties of Londrina and Assaí. Transect census were realized in each area, delimiting two 50 meter-paths, where the presence of bee in the open flowers was registered. Transects were run through for five minutes, every 15 minutes, starting at 1 pm and finishing at 8 pm. Results showed that the bumblebees present in the Northern Paraná region were the Xylocopa frontalis and X. suspecta, with the predominance of the first. Highest fructification rates occurred in the areas with more frequency of bumblebees and low frequency of cleptobiotic bees. Lowest fructification rates were due to the absence of bumblebees or high frequency of cleptobiotic bees

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Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

International Journal of Agronomy ◽

10.1155/2020/3205710 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Lydia K. Asande ◽

Omwoyo Ombori ◽

Evans N. Nyaboga ◽

Richard O. Oduor

Keyword(s):

Leaf Disc ◽

Genetic Fidelity ◽

Passion Fruit ◽

Nodal Explants ◽

Ms Medium ◽

Regeneration System ◽

Passiflora Edulis ◽

Tropical Fruit ◽

Srap Markers

Passion fruit (Passiflora edulis (Sims)) is currently ranked third among fruit exports from Kenya and has great potential since the demand for both fresh fruit and processed juice is on a continuous increase. Passion fruit production in Kenya is constrained by a lack of healthy, clean planting material, poor seed viability, and low germination rates. To address this, the present study reports an in vitro plant regeneration protocol for passion fruit using leaf disc and nodal explants and genetic fidelity analysis of the regenerated plants. The highest number of shoot regeneration was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg·L−1 6-Benzyl amino purine (BAP) (shoot induction medium). The multiplication of shoots was optimum in MS medium supplemented with 3 mg·L−1 BAP. To eliminate the requirement of an additional step of in vitro rooting, exogenous application of putrescine induced the formation and development of roots on nodal explants. Genetic fidelity analysis of the in vitro regenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity of the in vitro regenerated and macropropagated plants. The in vitro regeneration system developed can be utilized for mass clonal propagation for the economic commercial exploitation of this important tropical fruit.

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A simple and fast Agrobacterium-mediated transformation system for hybrid passion fruit (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa)

10.21203/rs.3.rs-27621/v1 ◽

2020 ◽

Author(s):

Lydia K. Asande ◽

Richard O. Omwoyo ◽

Richard O. Oduor ◽

Evans Nyaboga

Keyword(s):

Passion Fruit ◽

Transformation System ◽

Passiflora Edulis ◽

Agrobacterium Mediated Transformation

Abstract The authors have removed this preprint from Research Square.

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EMERGÊNCIA E DESENVOLVIMENTO DE PLÂNTULAS DE MARACUJÁ-AMARELO EM DIFERENTES SUBSTRATOS

ENERGIA NA AGRICULTURA ◽

10.17224/energagric.2019v34n01p18-27 ◽

2019 ◽

Vol 34 (01) ◽

pp. 18-27

Author(s):

Laís Gertrudes Fontana Silva ◽

Ramon Amaro de Sales ◽

Franciele Pereira Rossini ◽

Yago Tonini da Vitória ◽

Sávio Da Silva Berilli

Keyword(s):

Organic Matter ◽

Block Design ◽

Fruit Production ◽

Passion Fruit ◽

Vegetative Development ◽

Passiflora Edulis ◽

Randomized Block Design ◽

Rate Of Emergence ◽

Yellow Passion Fruit ◽

Coffee Roasting

EMERGÊNCIA E DESENVOLVIMENTO DE PLÂNTULAS DE MARACUJÁ-AMARELO EM DIFERENTES SUBSTRATOS LAÍS GERTRUDES FONTANA SILVA1; RAMON AMARO DE SALES2; FRANCIELE PEREIRA ROSSINI3; YAGO TONINI DA VITÓRIA1 E SÁVIO DA SILVA BERILLI1 1Instituto Federal do Espirito Santo – Campus Itapina, BR 259, KM 70, Colatina-ES, Brasil, [email protected]; [email protected]; [email protected] 2Departamento de Fitotecnia, Universidade Federal de Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, CEP: 36570 900, Viçosa - MG, Brasil, [email protected] 3Departamento de Cultura de Tecidos, UFES – campus São Mateus, Rodovia Governador Mário Covas, Km 60, Bairro Litorâneo, 29932-540, São Mateus - ES, Brasil, [email protected] RESUMO: O Brasil domina a produção do maracujazeiro-amarelo, abastecendo o mercado nacional e internacional com a sua fruta e polpa. Dentro da cadeia produtiva do maracujá, na etapa de produção de mudas, um fator importante que deve ser levado em consideração é o uso da matéria orgânica na composição dos substratos, devido as diversas características benéficas que o mesmo condiciona as mudas. Nesse sentido, o presente trabalho teve o objetivo avaliar a emergência e o desenvolvimento de mudas de Passiflora edulis f. flavicarpa cultivadas em substratos com diferentes fontes de matéria orgânica. O experimento foi conduzido em casa de vegetação, em delineamento de blocos casualizados, com seis repetições e seis tratamentos, contendo cinco fontes de matéria orgânica para a composição do substrato: lodo de curtume, resíduo de torrefação de café, esterco bovino curtido, resíduo de laticínio e composto urbano, além dos tratamentos sem uso de matéria orgânica (solo com e sem adubação). Foram avaliadas características de emergência e desenvolvimento das mudas, observando-se que houve maior índice de velocidade de emergência no tratamento com resíduo de torrefação de café, além de que todos os tratamentos com fontes orgânicas apresentaram respostas satisfatórias favorecendo o desenvolvimento vegetativo do maracujá-amarelo sendo recomendadas para a produção de mudas. Palavras-chaves: Passiflora edulis f. flavicarpa, Resíduos orgânicos, Sustentabilidade YELLOW PASSION FRUIT PLANTLETS EMERGENCY AND DEVELOPMENT IN DIFFERENT SUBSTRATES ABSTRACT: Brazil dominates yellow passion fruit production, supplying national and international market. In the stage of seedling production an important factor that must be taken into account is the use of organic matter in substrates composition, due to several beneficial characteristics it brings to plantlest development. The aim of this study was to evaluate the emergence and development of Passiflora edulis f. flavicarpa grown on different substrates with organic matter sources. The experiment was conducted in a greenhouse, in randomized block design, with six treatments and (tannery sludge, coffee roasting residue, tanned bovine manure, dairy and urban compost and the control - soil with and without fertilization) six replicates,. It was evaluated the emergence and emergence characteristics of the seedlings. The results showed that there was a higher rate of emergence speed in the treatment with coffee roasting residue, besides that all treatments with organic sources presented satisfactory responses favored the yellow passion fruit vegetative development being recommended for plantlets production. Keywords: Passiflora edulis f. flavicarpa, Organic waste, Sustainability

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Identificación molecular de aislamientos de Fusarium asociados a maracuyá en el Valle del Cauca, Colombia

Agronomía Mesoamericana ◽

10.15517/ma.v29i1.27114 ◽

2018 ◽

Vol 29 (1) ◽

pp. 53

Author(s):

Edwin David Henao-Henao ◽

Carlos Alberto Hernandez-Medina ◽

Claudia Salazar-González ◽

Martha Lucia Velasco-Belalcazar ◽

Eyder Daniel Gómez-López

Keyword(s):

Nearest Neighbor ◽

Fruit Production ◽

Similarity Coefficient ◽

Fusarium Proliferatum ◽

Passion Fruit ◽

Economic Losses ◽

Passiflora Edulis ◽

Genus Fusarium ◽

Pcr Products ◽

Valle Del Cauca

The passion fruit’s production in Colombia has shown great advances; however, phytosanitary diseases remain as issues in passion fruit production. This includes the pathogens that hinder the establishment and development of the crop, such as some species of the genus Fusarium, pathogen considered as an important one because of the economic losses that it generates. The objective of this work was to molecularly identificate the populations of Fusarium associated with passion fruit (Passiflora edulis). Between January and December 2015, an isolation of 35 samples based on different affected organs of the plant and from localities found in five municipalities was performed in Valle del Cauca, Colombia. Eight isolates were selected and purified in order to perform their DNA’s extraction and also an amplification of region TEF 1 alpha. The PCR products were sequenced and compared with the NCBI database and Fusarium ID. The results obtained by sequencing showed that the isolates belong to Fusarium incarnatum, Fusarium solani, and Fusarium proliferatum, the first species had a mayor occurrence in the tested samples. The analysis of phylogenetic relationships was achieved by using the 6 MEGA program, and by using the similarity coefficient nearest neighbor, which allowed to observe the variability among the evaluated isolates.

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PRODUÇÃO DO MARACUJAZEIRO-AMARELO IRRIGADO COM ÁGUA SALINA EM COVAS PROTEGIDAS CONTRA PERDAS HÍDRICAS

Irriga ◽

10.15809/irriga.2005v10n3p229-240 ◽

2005 ◽

Vol 10 (3) ◽

pp. 229-240 ◽

Cited By ~ 3

Author(s):

Lourival Ferreira Cavalcante ◽

José Ronaldo Medeiros Costa ◽

Fernando Kidelmar Dantas de Oliveira ◽

Italo Helbert Lucena Cavalcante ◽

Fernanda Aspazia Rodrigues de Araújo

Keyword(s):

Arid Zone ◽

Saline Water ◽

Irrigation System ◽

Rio Grande ◽

Fruit Production ◽

Passion Fruit ◽

Santa Cruz ◽

Passiflora Edulis ◽

High Salinity Water ◽

Yellow Passion Fruit

PRODUÇÃO DO MARACUJAZEIRO-AMARELO IRRIGADO COM ÁGUA SALINA EM COVAS PROTEGIDAS CONTRA PERDAS HÍDRICAS Lourival Ferreira Cavalcante1; José Ronaldo Medeiros Costa2; Fernando Kidelmar Dantas de Oliveira3; Ítalo Herbert Lucena Cavalcante4; Fernanda Aspazia Rodrigues de Araújo21DSER/CCA/Universidade Federal da Paraiba, Areia, PB, [email protected]/ Universidade Federal da Paraiba, Areia-PB3Prodesiano/Banco do Nordeste do Brasil, Santa Cruz – RN4Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP 1 RESUMO Um experimento de campo foi conduzido no município de Santa Cruz, zona semi-árida do Rio Grande do Norte, para avaliar o comportamento produtivo do maracujazeiro-amarelo, Passiflora edulis f. flavicarpa Deg., e a salinidade do solo irrigados com água salina. Os tratamentos foram dispostos em blocos casualizados, com quatro repetições e 15 plantas por parcela, em esquema fatorial 5 x 2, referente ao revestimento das faces laterais das covas com filme de polietileno (0, 1, 2, 3 e 4 faces), irrigadas diariamente por gotejamento com 5 e 10 litros de água de condutividade elétrica 3,2 dSm-1, do tipo C4S3, fortemente salina. O plantio foi feito no espaçamento de 2m entre linhas e 4m nas linhas, usando espaldeira com um arame liso nº 12 instalado a 2m de altura. A interação volumes de água x revestimento das covas não exerceu efeito significativo sobre nenhuma das variáveis estudadas, mas o revestimento das faces laterais das covas com filme de polietileno contribuiu para o aumento do número de frutos colhidos, produção por planta, e manteve o solo mais úmido e com menor índice de salinidade. UNITERMOS: Irrigação, Passiflora, sistema de produção. CAVALCANTE, L. F.; COSTA, J. R. M.; OLIVEIRA, F. K. D.; CAVALCANTE, I. H. L.; ARAUJO, F. A. R. YELLOW PASSION FRUIT PRODUCTION IRRIGATED WITH SALINE WATER IN COVERED HOLES TO REDUCE WATER LOSS 2 ABSTRACT This study aimed to evaluate the production of yellow passion fruit plants, Passiflora edulis f. flavicarpa Deg., and the soil salinity when irrigated with saline water. The experiment was carried out in Santa Cruz, located in the semi arid zone of the state of Rio Grande do Norte, Brazil. The treatments were distributed in randomized blocks, with four replications and 15 plants in each plot, using a factorial design 5 x 2, which referred to the lateral covered cave with polyethylene film (0, 1, 2, 3 and 4 sides). The plants were irrigated daily using a drip irrigation system with 5 and 10 liters of high salinity water and electrical conductivity of 3.2 dSm-1. Planting distances were 2m between lines and 4m between plants, using number 12 flat wire shoulder-piece at the height of 2m. Lateral cave covering had a positive effect on fruit number, production by plant and yield of yellow passion fruit and contributed to keep the soil the more humid and with lower salinity rate.. KEYWORDS: Irrigation, Passiflora, production system.

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Establishment of a Novel and Efficient Agrobacterium-Mediated in Planta Transformation System for Passion Fruit (Passiflora edulis)

Plants ◽

10.3390/plants10112459 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2459

Author(s):

Hafiz Muhammad Rizwan ◽

Qiang Yang ◽

Ahmed Fathy Yousef ◽

Xiaoxue Zhang ◽

Yasir Sharif ◽

...

Keyword(s):

Virulent Strain ◽

Crop Improvement ◽

Multiple Shoots ◽

Passion Fruit ◽

In Planta Transformation ◽

Transformation System ◽

In Planta ◽

Labor Costs ◽

Passiflora Edulis ◽

Functional Studies

Passion fruit (Passiflora edulis) is an important fruit crop with high economic value. Genetic engineering plays an important role in crop improvement with desired traits and gene functional studies. The lack of a simple, efficient, and stable transformation system for passion fruit has greatly limited gene functional studies. In this study, a simple and efficient Agrobacterium-mediated in planta transformation system for passion fruit was established, using Agrobacterium virulent strain EHA105 harboring the binary vectors pCAMBIA1301 and pCAMBIA1302 with GUS and GFP reporter genes. The system requires less time and labor costs than conventional transformation systems, and no additional phytohormones and sterile conditions are required. Regeneration efficiency of 86% and transformation efficiency of 29% were achieved, when the wounds were wrapped with Parafilm and the plants were kept in darkness for 15 days. Approximately 75% of the regenerated plants had a single shoot and 26% multiple shoots. The transformation was confirmed at the DNA and RNA levels as well as by GUS staining and GFP fluorescent measurements. The developed protocol will contribute to the genetic improvement of passion fruit breeding.

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Passion fruit and apple: from residues to antioxidant, antimicrobial and anti-Alzheimer’s potential

Ciência Rural ◽

10.1590/0103-8478cr20180076 ◽

2018 ◽

Vol 48 (9) ◽

Author(s):

Denise Simim Lima ◽

Nathália Barroso Almeida Duarte ◽

Débora Luiza Costa Barreto ◽

Geane Pereira de Oliveira ◽

Jacqueline Aparecida Takahashi ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Malus Domestica ◽

Fruit Production ◽

Passion Fruit ◽

Natural Antimicrobial ◽

Passiflora Edulis ◽

Production Chain ◽

Functional Product ◽

Fuji Apple

ABSTRACT: The waste in the fruit production chain, including the juice and pulp industries, produces large quantities of leftover husks, seeds and bagasse. This volume of waste generates huge environmental and economic impact. The objective of this research was to determine the potential of using residues from passion fruit (Passiflora edulis) and apple (Malus domestica) varieties in the production of functional flours. Passion fruit flour showed greater reduction of DPPH (EC50%: 50.4μg/mL) radicals, showing antioxidant potential, as well as a more efficient inhibition of Staphylococcus aureus (71.3±1.2μg/mL), with a modest; however efficient, inhibition of acetylcholinesterase (10%). All Apple flours were good antioxidants and the fuji apple flour stood out inhibiting Pseudomonas aeruginosa (78.6±3.1μg/mL). All the residues showed potential for use as a functional product either as a source of antioxidants, a natural (antimicrobial) preservative for dry foods or supplementary use by patients with Alzheimer’s disease.

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