scholarly journals Passion fruit and apple: from residues to antioxidant, antimicrobial and anti-Alzheimer’s potential

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Denise Simim Lima ◽  
Nathália Barroso Almeida Duarte ◽  
Débora Luiza Costa Barreto ◽  
Geane Pereira de Oliveira ◽  
Jacqueline Aparecida Takahashi ◽  
...  

ABSTRACT: The waste in the fruit production chain, including the juice and pulp industries, produces large quantities of leftover husks, seeds and bagasse. This volume of waste generates huge environmental and economic impact. The objective of this research was to determine the potential of using residues from passion fruit (Passiflora edulis) and apple (Malus domestica) varieties in the production of functional flours. Passion fruit flour showed greater reduction of DPPH (EC50%: 50.4μg/mL) radicals, showing antioxidant potential, as well as a more efficient inhibition of Staphylococcus aureus (71.3±1.2μg/mL), with a modest; however efficient, inhibition of acetylcholinesterase (10%). All Apple flours were good antioxidants and the fuji apple flour stood out inhibiting Pseudomonas aeruginosa (78.6±3.1μg/mL). All the residues showed potential for use as a functional product either as a source of antioxidants, a natural (antimicrobial) preservative for dry foods or supplementary use by patients with Alzheimer’s disease.

2017 ◽  
Vol 3 (1) ◽  
pp. 12
Author(s):  
Humberto Godoy Androcioli ◽  
Ayres de Oliveira Menezes Junior ◽  
Ana Odete Santos Vieira ◽  
Dayanne Fabricio Bressan ◽  
Adriano Thibes Hoshino ◽  
...  

The yellow passion fruit (Passiflora edulis f. flavicarpa) needs pollinators for fructification due to the existence of mechanisms that impede self-pollinating. Pollinators of great efficiency for the yellow passion fruit in Brazil are the bumblebee species (Xylocopa frontalis, X. grisescens and X. Suspecta). Deforestation by agricultural expansion in the region has a negative impact on these pollinators’ populations, leaving space for the action of cleptobiotic species with further damage to fruit production. The objective of this study was to evaluate the frequency of pollinating bees and floral visitors during yellow passion fruit culture’s post anthesis period in Northern Paraná and its fructification effects. Three passion fruit plantations in Northern Paraná were evaluated in the counties of Londrina and Assaí. Transect census were realized in each area, delimiting two 50 meter-paths, where the presence of bee in the open flowers was registered. Transects were run through for five minutes, every 15 minutes, starting at 1 pm and finishing at 8 pm. Results showed that the bumblebees present in the Northern Paraná region were the Xylocopa frontalis and X. suspecta, with the predominance of the first. Highest fructification rates occurred in the areas with more frequency of bumblebees and low frequency of cleptobiotic bees. Lowest fructification rates were due to the absence of bumblebees or high frequency of cleptobiotic bees


2019 ◽  
Vol 2 (1) ◽  
pp. 07-12
Author(s):  
Sony Eka Nugraha ◽  
Suryadi Achmad ◽  
Erly Sitompul

North Sumatera is one of the central areas of purple passion fruit production in Indonesia. Processing passion fruit into beverage products (passion fruit juice) produces peel  has not been utilized. The use of passion fruit skin needs to be studied so that it can be useful as a raw material for antibacterial drug preparations. The aim of this study was to determine the phytochemical constituent screening  and antibacterial activity of ethyl acetate fraction of purple passion fruit peel against Staphylococcus aureus and Escherichia coli. Simplicia and ethyl acetate fraction were determinated  its phytochemical properties.  The extraction process by percolation method using ethanol  96% and continue to fractionation process by liquid liquid extraction method using n-hexane and ethyl acetate. The antibacterial activity were tested  against Staphylococcus aureus and Escherichia coli using agar diffusion method with paper discs. The result showed  that  the simplicia characteristic of passion fruit peel  were water content of  8.64%, water soluble extract of  31.69%, ethanol soluble extract of 13.02%, ash total of 7.89%, and insoluble ash in acid of 0.816%. The phytochemical screening simplicia and ethyl acetate fraction showed the presence of flavonoids, glycosides, saponins and tannins. The antibacterial activity test showed that the ethyl acetate fraction has an effective inhibition at the concentration of 100 mg/ml against Staphylococcus aureus and Escherichia coli, it showed dose dependent manner. The ethyl acetate fraction of passion fruit peel (Passiflora edulis Sims) has an antibacterial activity  on Staphylococcus aureus and Escherichia coli


2020 ◽  
Vol 42 (5) ◽  
Author(s):  
Aline Oliveira Zacharias ◽  
Fábio Gelape Faleiro ◽  
Gabriella Queiroz de Almeida

Abstract Productivity of passion fruit in Brazil varies widely among producers in different states and regions depending on the adoption of technologies. In this study, the goal was to evaluate the producers profile and use of technologies in passion fruit production in the Triângulo Mineiro region, driven by the local juice industry. The research was carried out from 2018 to 2019, using Behavioral Diagnosis of Productive Activity methodology, based on questionnaires applied to 22 producers in eight cities. The data were submitted to descriptive and multivariate statistical analysis. Most producers grow passion fruit in up to 5 ha, in a family work regimen, and have little experience. The cultivation system is open field in vertical trellis, with a production cycle of 1.5 years. The great majority use manual pollination, chemical and organic fertilization, and do not systematically adopt integrated phytosanitary management. The seedlings are purchased from qualified nurseries and the most planted cultivar is the FB 300. The main losses reported by producers were caused by drought and damage to floral bud, with an average fruit yield of 17 t ha-1 year-1. This production chain is structured but requires technical interventions to increase the profitability and sustainability of the production system.


Author(s):  
Famubo, Joseph A. ◽  
Isiaka, Aishatu ◽  
Abbas, Yusuf B.

The hygiene status of most abattoirs and retail outlets in Nigeria is very poor, and it contributes to the unacceptable level of bacteria load in beef, and poses a health risk to consumers. This study examined the bacteriological analysis of beef production chain in Birnin Kebbi metropolis. A total of 100 samples of meat, water and surface swab in Main Market, GRA, Badariya, Bayan Kara and Rafin Atiku, Birnin Kebbi Central abattoir and retail meat shops were collected aseptically, processed and analyzed. Meat from retail outlets were mostly contaminated by Staphylococcus aureus (24.4%), followed by Escherichia coli (17.3%), Klebsiella pneumonia (9.8%), Salmonella typhimurium (9.1%), Entrobater aerogenes (8.5%), Streptococcus pyogenes (8.1%), Proteus vulgaris (7.8%), Micrococcus luteus (7.8%) and Pseudomonas aeruginosa (7.2%). In the abattoirs contact surfaces, contamination occurred mostly by Staphylococcus aureus (23.4%), Salmonella typhimurium (18.2%), Escherichia coli (16.9%), Entrobater aerogenes (9.1%), Streptococcus pyogenes (7.8%), Klebsiella pneumonia, Proteus vulgaris, and Pseudomonas aeruginosa (6.5% each) and Micrococcus luteus (5.2%). The findings revealed a high bacterial load in both abattoir and retail meat outlets. In order to safeguard the health of the public against the risks of food borne infections, there is a need to educate and advocate good sanitation and meat handling practices in the abattoir and beef retail outlets.


Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Lydia K. Asande ◽  
Richard O. Omwoyo ◽  
Richard O. Oduor ◽  
Evans N. Nyaboga

Abstract Background Passion fruit (Passiflora edulis Sims) is an important horticultural crop in the tropics and subtropics, where it has great commercial potential due to high demand for fresh edible fruits and processed juice as well as source of raw materials in cosmetic industries. Genetic engineering shows great potential in passion fruit improvement and can compensate for the limitations of conventional breeding. Despite the success achieved in genetic modification of few passion fruit varieties, transgenic passion fruit production is still difficult for farmer-preferred cultivars. Therefore, it is important to establish a simple and fast Agrobacterium-mediated cell transformation of commercial hybrid passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa). Results In the present study, we have developed a simple and fast Agrobacterium-mediated transformation system for hybrid passion fruit KPF4 using leaf disc explants. Factors affecting the rate of transient beta (β)-glucuronidase (gusA) expression and consequently transformation efficiency were optimized as follows: Agrobacterium cell density with an OD600 of 0.5, 30 min infection time, 3 days of co-cultivation duration and the incorporation of 200 µM acetosyringone into Agrobacterium infection suspension medium. Using the optimized conditions, transgenic plants of KPF4 were produced within 2 months with an average transformation efficiency of 0.67%. The β-glucuronidase (GUS) histochemical staining confirmed the expression and integration of an intron-containing gusA gene into transformed leaf discs and transgenic plant lines of KPF4. The presence of gusA gene in the transgenic plants was confirmed by polymerase chain reaction (PCR). The results confirmed that the gusA gene was efficiently integrated into the passion fruit genome. Conclusions The developed transformation protocol is simple and rapid and could be useful for functional genomic studies and transferring agronomically important traits into passion fruit hybrid KPF4. This study developed a method that can be used to transfer traits such as resistance to viral diseases, low fruit quality and short storage life. To the best of our knowledge, this is the first report on genetic transformation system for commercial passion fruit hybrid KPF4.


2019 ◽  
Vol 34 (01) ◽  
pp. 18-27
Author(s):  
Laís Gertrudes Fontana Silva ◽  
Ramon Amaro de Sales ◽  
Franciele Pereira Rossini ◽  
Yago Tonini da Vitória ◽  
Sávio Da Silva Berilli

EMERGÊNCIA E DESENVOLVIMENTO DE PLÂNTULAS DE MARACUJÁ-AMARELO EM DIFERENTES SUBSTRATOS   LAÍS GERTRUDES FONTANA SILVA1; RAMON AMARO DE SALES2; FRANCIELE PEREIRA ROSSINI3; YAGO TONINI DA VITÓRIA1 E SÁVIO DA SILVA BERILLI1   1Instituto Federal do Espirito Santo – Campus Itapina, BR 259, KM 70, Colatina-ES, Brasil, [email protected]; [email protected]; [email protected] 2Departamento de Fitotecnia, Universidade Federal de Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, CEP: 36570 900, Viçosa - MG, Brasil, [email protected] 3Departamento de Cultura de Tecidos, UFES – campus São Mateus, Rodovia Governador Mário Covas, Km 60, Bairro Litorâneo, 29932-540, São Mateus - ES, Brasil, [email protected]   RESUMO: O Brasil domina a produção do maracujazeiro-amarelo, abastecendo o mercado nacional e internacional com a sua fruta e polpa. Dentro da cadeia produtiva do maracujá, na etapa de produção de mudas, um fator importante que deve ser levado em consideração é o uso da matéria orgânica na composição dos substratos, devido as diversas características benéficas que o mesmo condiciona as mudas. Nesse sentido, o presente trabalho teve o objetivo avaliar a emergência e o desenvolvimento de mudas de Passiflora edulis f. flavicarpa cultivadas em substratos com diferentes fontes de matéria orgânica. O experimento foi conduzido em casa de vegetação, em delineamento de blocos casualizados, com seis repetições e seis tratamentos, contendo cinco fontes de matéria orgânica para a composição do substrato: lodo de curtume, resíduo de torrefação de café, esterco bovino curtido, resíduo de laticínio e composto urbano, além dos tratamentos sem uso de matéria orgânica (solo com e sem adubação). Foram avaliadas características de emergência e desenvolvimento das mudas, observando-se que houve maior índice de velocidade de emergência no tratamento com resíduo de torrefação de café, além de que todos os tratamentos com fontes orgânicas apresentaram respostas satisfatórias favorecendo o desenvolvimento vegetativo do maracujá-amarelo sendo recomendadas para a produção de mudas.   Palavras-chaves: Passiflora edulis f. flavicarpa, Resíduos orgânicos, Sustentabilidade   YELLOW PASSION FRUIT PLANTLETS EMERGENCY AND DEVELOPMENT IN DIFFERENT SUBSTRATES   ABSTRACT: Brazil dominates yellow passion fruit production, supplying national and international market. In the stage of seedling production  an important factor that must be taken into account is the use of organic matter in substrates composition, due to several beneficial characteristics it brings to plantlest development. The aim of this study was to evaluate the emergence and development of Passiflora edulis f. flavicarpa grown on different substrates with organic matter sources. The experiment was conducted in a greenhouse, in randomized block design, with six treatments and (tannery sludge, coffee roasting residue, tanned bovine manure, dairy and urban compost and the control - soil with and without fertilization) six replicates,. It was evaluated the emergence and emergence characteristics of the seedlings. The results showed that there was a higher rate of emergence speed in the treatment with coffee roasting residue, besides that all treatments with organic sources presented satisfactory responses favored the yellow passion fruit vegetative development being recommended for plantlets production.   Keywords: Passiflora edulis f. flavicarpa, Organic waste, Sustainability


2020 ◽  
Vol 26 (1) ◽  
pp. 22-25
Author(s):  
Iif Hanifa Nurrosyidah ◽  
Ni Made Mertaniasih ◽  
Isnaeni Isnaeni

This study aims to determine the minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) of the fermentation filtrate (FF) on red passion fruit pulp (Passiflora edulis Sims.) against Escherichia coli Extended Spectrum Beta Lactamase (ESBL) and Methicillin Resistant Staphylococcus aureus (MRSA). The method used was the fermentation of red passion fruit pulp for 24 h using De Man Rogosa Sharpe Broth (MRS-broth) media, and the result showed that the FF of red passion fruit pulp made a dilution series concentration of 50 percent, 25 percent, 12.5 percent, 6.25 percent. Considering this result, the MIC FF of red passion fruit cells against ESBL and MRSA has a 25 percent dilution concentration, while MBC has a 50 percent dilution concentration. This shows that red passion fruit has the potential to be developed as antibacterial material, especially to fight bacteria that are already resistant.


2018 ◽  
Vol 29 (1) ◽  
pp. 53
Author(s):  
Edwin David Henao-Henao ◽  
Carlos Alberto Hernandez-Medina ◽  
Claudia Salazar-González ◽  
Martha Lucia Velasco-Belalcazar ◽  
Eyder Daniel Gómez-López

The passion fruit’s production in Colombia has shown great advances; however, phytosanitary diseases remain as issues in passion fruit production. This includes the pathogens that hinder the establishment and development of the crop, such as some species of the genus Fusarium, pathogen considered as an important one because of the economic losses that it generates. The objective of this work was to molecularly identificate the populations of Fusarium associated with passion fruit (Passiflora edulis). Between January and December 2015, an isolation of 35 samples based on different affected organs of the plant and from localities found in five municipalities was performed in Valle del Cauca, Colombia. Eight isolates were selected and purified in order to perform their DNA’s extraction and also an amplification of region TEF 1 alpha. The PCR products were sequenced and compared with the NCBI database and Fusarium ID. The results obtained by sequencing showed that the isolates belong to Fusarium incarnatum, Fusarium solani, and Fusarium proliferatum, the first species had a mayor occurrence in the tested samples. The analysis of phylogenetic relationships was achieved by using the 6 MEGA program, and by using the similarity coefficient nearest neighbor, which allowed to observe the variability among the evaluated isolates.


Irriga ◽  
2005 ◽  
Vol 10 (3) ◽  
pp. 229-240 ◽  
Author(s):  
Lourival Ferreira Cavalcante ◽  
José Ronaldo Medeiros Costa ◽  
Fernando Kidelmar Dantas de Oliveira ◽  
Italo Helbert Lucena Cavalcante ◽  
Fernanda Aspazia Rodrigues de Araújo

PRODUÇÃO DO MARACUJAZEIRO-AMARELO IRRIGADO COM ÁGUA SALINA EM COVAS PROTEGIDAS CONTRA PERDAS HÍDRICAS  Lourival Ferreira Cavalcante1; José Ronaldo Medeiros Costa2; Fernando Kidelmar Dantas de Oliveira3; Ítalo Herbert Lucena Cavalcante4; Fernanda Aspazia Rodrigues de Araújo21DSER/CCA/Universidade Federal da Paraiba, Areia, PB, [email protected]/ Universidade Federal da Paraiba, Areia-PB3Prodesiano/Banco do Nordeste do Brasil, Santa Cruz – RN4Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP  1        RESUMO Um experimento de campo foi conduzido no município de Santa Cruz, zona semi-árida do Rio Grande do Norte, para avaliar o comportamento produtivo do maracujazeiro-amarelo, Passiflora edulis f. flavicarpa Deg., e a salinidade do solo irrigados com água salina. Os tratamentos foram dispostos em blocos casualizados, com quatro repetições e 15 plantas por parcela, em esquema fatorial 5 x 2, referente ao revestimento das faces laterais das covas com filme de polietileno (0, 1, 2, 3 e 4 faces), irrigadas diariamente por gotejamento com 5 e 10 litros de água de condutividade elétrica 3,2 dSm-1, do tipo C4S3, fortemente salina. O plantio foi feito no espaçamento de 2m entre linhas e 4m nas linhas, usando espaldeira com um arame liso nº 12 instalado a 2m de altura. A interação volumes de água x revestimento das covas não exerceu efeito significativo sobre nenhuma das variáveis estudadas, mas o revestimento das faces laterais das covas com filme de polietileno contribuiu para o aumento do número de frutos colhidos, produção por planta, e manteve o solo mais úmido e com menor índice de salinidade. UNITERMOS: Irrigação, Passiflora, sistema de produção.  CAVALCANTE, L. F.; COSTA, J. R. M.; OLIVEIRA, F. K. D.; CAVALCANTE, I. H. L.; ARAUJO, F. A. R. YELLOW PASSION FRUIT PRODUCTION IRRIGATED WITH SALINE WATER IN COVERED HOLES TO REDUCE WATER LOSS  2        ABSTRACT This study aimed to evaluate the production of yellow passion fruit plants, Passiflora edulis f. flavicarpa Deg., and the soil salinity when irrigated with saline water. The experiment was carried out in Santa Cruz, located in the semi arid zone of the state of  Rio Grande do Norte, Brazil. The treatments were distributed in randomized blocks, with four replications and 15 plants in each plot, using a factorial design 5 x 2, which referred to the lateral covered cave with polyethylene film (0, 1, 2, 3 and 4 sides). The plants were irrigated daily using a drip irrigation system with 5 and 10 liters of high salinity water and electrical conductivity of 3.2 dSm-1. Planting distances were 2m between lines and 4m between plants, using number 12 flat wire shoulder-piece at the  height of 2m. Lateral cave covering had a positive effect on fruit number, production by plant and yield of yellow passion fruit and contributed to keep the soil the more humid and with lower salinity rate.. KEYWORDS: Irrigation, Passiflora, production system.   


2020 ◽  
Vol 87 ◽  
Author(s):  
Addolorata Colariccio ◽  
Leilane Karam Rodrigues ◽  
Alyne de Fátima Ramos ◽  
Alexandre Levi Rodrigues Chaves ◽  
Marcelo Eiras ◽  
...  

ABSTRACT: CABMV is a limiting virus for passion fruit crop in Brazil, its main producing country. This virus has been reported in all producing states of the country, with the state of Santa Catarina (SC) in 2017 standing as the third largest passion fruit producer. In 2017, it reached 8.4% of the national production. The southern coast is the main responsible for the increase in production, which has been supplying the domestic market. However, in that same year, this region recorded the first symptom expressions in plants and fruits. The evaluation of the samples collected in the municipalities of Sombrio, Praia Grande and São João do Sul, southern coast of SC, was performed by using a mechanical transmission to indicator plants, PTA-ELISA and RT-PCR, and by sequencing. The evaluation results were positive for CABMV and negative for CMV in PTA-ELISA. In RT-PCR, there was the formation of a 700bp ca band, expected size for Potyvirus, whose sequence comparison with those deposited in GenBank reveled 98% identity with the isolates from São Paulo State. The occurrence of the virus in the southern coast of SC did not reach a serious decrease in passion fruit production due to the union of producers, who adopted preventive management measures to control the virus, whose effect led to a consolidation of the passion fruit production chain in the region.


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