scholarly journals Acceleration of bone union by in situ-formed hydrogel containing bone morphogenetic protein-2 in a mouse refractory fracture model

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Shintaro Shoji ◽  
Kentaro Uchida ◽  
Wataru Satio ◽  
Hiroyuki Sekiguchi ◽  
Gen Inoue ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Repair ◽  
Fracture Site ◽  
Bone Union ◽  
Fracture Model ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
And Control ◽  
Gene Expression Levels

Abstract Background An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHs). Several studies have reported the application of IFHs in cell delivery and tissue engineering. IFHs may also be ideal carrier materials for bone repair, although their potential as a carrier for bone morphogenetic protein (BMP)-2 has yet to be examined. Here, we examined the effect of an IFH made of hyaluronic acid (IFH-HA) containing BMP-2 in promoting osteogenesis in a mouse refractory fracture model. Methods Immediately following a fracture procedure, animals either received no treatment (control) or an injection of IFH-HA/PBS or IFH-HA containing 2 μg BMP-2 (IFH-HA/BMP-2) into the fracture site (n = 16, each treatment). Results Fracture sites injected with IFH-HA/BMP-2 showed significantly greater bone volume, bone mineral content, and bone union compared with sites receiving no treatment or treated with IFH-HA/PBS alone (each n = 10). Gene expression levels of osteogenic markers, Alpl, Bglap, and Osx, were significantly raised in the IFH-HA/BMP-2 group compared to the IFH-HA/PBS and control groups (each n = 6). Conclusion IFH-HA/BMP-2 may contribute to the treatment of refractory fractures.

Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model

2021 ◽  
pp. 1-9
Author(s):  
Shintaro Shoji ◽  
Kentaro Uchida ◽  
Ryo Tazawa ◽  
Wataru Saito ◽  
Akiyoshi Kuroda ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Bone Defect ◽  
Bone Repair ◽  
Critical Size ◽  
Bone Morphogenetic Protein 2 ◽  
Micro Computed Tomography ◽  
Defect Model ◽  
Morphogenetic Protein

BACKGROUND: An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHGs). IFHGs may also be ideal carrier materials for bone repair, although their ability to carry bone morphogenetic protein-2 (BMP2) has yet to be examined. OBJECTIVE: We examined the effectiveness of an IFHG made of hyaluronan (IFHG-HA) containing BMP2 for promoting bone formation in a mouse critical size bone defect model. METHODS: C57/BL6J mice received a 2-mm femoral critical-sized bone defect before being randomly assigned to one of the following treatment groups (n = 6): control (no treatment), IFHG-HA only, PBS with BMP2, and IFHG-HA with BMP2. X-ray radiographs were utilized to track new bone formation, and micro-computed tomography and histological examination were performed on new bone formed at the bone defect site two weeks after surgery. RESULTS: Mice treated with PBS with BMP2 and IFHG-HA with BMP2 had greater bone volume (BV) and bone mineral content (BMC) than those receiving control, and successfully achieved consolidation. Mice treated with IFHG-HA with BMP2 had significantly higher BV and BMC than those treated with PBS with BMP2. CONCLUSIONS: IFHG-HA may be an effective carrier for BMP2 to enable delivery for bone defect repair.

scholarly journals A Comparative Genomic and Transcriptional Survey Providing Novel Insights into Bone Morphogenetic Protein 2 (bmp2) in Fishes

2019 ◽  
Vol 20 (24) ◽  
pp. 6137 ◽  
Author(s):  
Guang Yang ◽  
Zhendong Qin ◽  
Hongyan Kou ◽  
Rishen Liang ◽  
Lijuan Zhao ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Repair ◽  
Fish Bone ◽  
Transcriptomic Analysis ◽  
Bone Morphogenetic Protein 2 ◽  
Comparative Genomic ◽  
Sequence Alignments ◽  
Cell Proliferation And Differentiation ◽  
Morphogenetic Protein

Intermuscular bones (IBs) are only found in the muscles of fish. Bone morphogenetic protein 2 (bmp2) is considered to be the most active single osteogenesis factor. It promotes cell proliferation and differentiation during bone repair, as well as inducing the formation of bones and cartilages in vivo. However, detailed investigations of this family in fish are incredibly limited. Here, we have used a variety of published and unpublished bmp2 sequences for teleosts and cartilage fish in order to explore and expand our understanding of bmp2 genes in fish. Our results confirmed that teleost genomes contain two or more bmp2 genes, and the diversity of bmp2 genes in vertebrates appears to be as a result of a combination of whole genome duplication (WGD) and gene loss. Differences were also observed in tissue distribution and relative transcription abundance of the bmp2s through a transcriptomic analysis. Our data also indicated that bmp2b may play an important role in the formation of IBs in teleosts. In addition, protein sequence alignments and 3D structural predictions of bmp2a and bmp2b supported their similar roles in fishes. To summarize, our existing work provided novel insights into the bmp2 family genes in fishes through a mixture of comparative genomic and transcriptomic analysis.

Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair

2011 ◽  
Vol 27 (5) ◽  
pp. 971-977 ◽  
Author(s):  
Anderson Paim Rosa ◽  
Luiz Gustavo de Sousa ◽  
Simone Cecilio Hallak Regalo ◽  
João Paulo Mardegan Issa ◽  
Ana Paula Amorim Barbosa ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Laser Irradiation ◽  
Bone Repair ◽  
Human Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Level Laser ◽  
Human Bone Morphogenetic Protein ◽  
Low Level Laser Irradiation ◽  
Recombinant Human Bone

scholarly journals Mesenchymal Stem Cell-mediated Gene Delivery of Bone Morphogenetic Protein-2 in an Articular Fracture Model

2007 ◽  
Vol 15 (8) ◽  
pp. 1543-1550 ◽  
Author(s):  
Terri Zachos ◽  
Alisha Diggs ◽  
Steven Weisbrode ◽  
Jeffrey Bartlett ◽  
Alicia Bertone
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Fracture Model ◽  
Bone Morphogenetic Protein 2 ◽  
Articular Fracture ◽  
Morphogenetic Protein

scholarly journals Bone Regeneration of Peri-Implant Defects Using a Collagen Membrane as a Carrier for Recombinant Human Bone Morphogenetic Protein-2

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Yoo-Kyung Sun ◽  
Jae-Kook Cha ◽  
Daniel Stefan Thoma ◽  
So-Ra Yoon ◽  
Jung-Seok Lee ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Control Group ◽  
Collagen Membrane ◽  
Treatment Groups ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Bone To Implant Contact ◽  
And Control

This study is designed to determine the effect of collagen membrane (CM) soaked with bone morphogenetic protein-2 (rhBMP-2) for the treatment of peri-implant dehiscence defects. Material and Methods. Three treatment groups were allocated at each defect in 5 dogs: (i) collagenated synthetic bone (OC) and CM soaked with rhBMP-2 (BMP group), (ii) OC and CM soaked with saline (nonBMP group), and (iii) no further treatment (control group). Titanium pins were used to stabilize the membranes in two dogs. Radiographic and histomorphometric analyses were performed 4 weeks later. Results. The median augmented volumes were 4.27 mm3, 6.24 mm3, and 2.75 mm3 in the BMP, nonBMP, and control groups, respectively; the corresponding median first bone-to-implant contact (fBIC) distances were 3.25 mm, 3.08 mm, and 2.56 mm (P>0.05). The placement of pins (with the BMP and nonBMP groups pooled) significantly improved bone regeneration: the augmented volumes were 17.60 mm3 with pins and 3.68 mm3 without pins (P=0.024), with corresponding fBIC distances of 2.25 mm and 3.31 mm, respectively (P<0.001). Conclusions. The addition of rhBMP-2 to CM failed to improve bone regeneration of peri-implant dehiscence defects compared to using an unsoaked CM after 4 weeks. However, the stabilization of CMs using pins positively influenced the outcomes.

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