scholarly journals Novel bone repairing scaffold consisting of bone morphogenetic Protein-2 and human Beta Defensin-3

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Wei He ◽  
Daixu Wei ◽  
Jun Zhang ◽  
Xiaonan Huang ◽  
Da He ◽  
...  
Keyword(s):  
Cell Growth ◽  
Osteogenic Differentiation ◽  
Bone Morphogenetic Protein ◽  
Slow Release ◽  
Three Dimensional ◽  
Antibacterial Properties ◽  
Bone Morphogenetic Protein 2 ◽  
Level Of Evidence ◽  
Morphogenetic Protein ◽  
Long Time

Abstract Background Synthetic biomaterials assist in modulating the vascular response in an injured bone by serving as delivery vehicles of pro-angiogenic molecules to the site of injury or by serving as mimetic platforms which offer support to cell growth and proliferation. Methods This study applied natural phospholipid modified protein technologies together with low temperature three-dimensional printing technology to develop a new model of three-dimensional artificial bone scaffold for potential use in repairing body injuries. The focus was to create a porous structure (PS) scaffold of two components, Bone Morphogenetic Protein-2 and Human Beta Defensin-3 (BMP2 and hBD3), which can synchronously realize directional bone induction, angiogenesis and postoperative antibacterial effects. BMP2 induces osteogenesis, whereas hBD3 is antibacterial. Results Our data showed that in the BMP2-hBD3-PS or hBD3-PS scaffolds, BMP2 had a slow-release rate of about 40% in 30 days, ensuring that BMP2 could penetrate into stem cells for osteogenic differentiation for a long time. The scaffolds promoted cell growth when in combination with BMP2, thus showing its importance in promoting cell growth. Alkaline Phosphatase (ALP) staining showed that the ALP content of BMP2-hBD3-PS and BMP2-PS had a significant increase in samples that contained BMP2, thus showing that these scaffolds promoted osteogenic differentiation. In all the constructs that had hBD3, they displayed antibacterial properties with hBD3, having a slow release of about 35% in 30 days, thus ensuring they provided protection. Conclusion Based on this study, the 3D printed BMP2 scaffolds show a great potential for the development of biodegradable bone implants. Level of evidence Level II, experimental comparative design.

Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy

Nanoscale ◽  
2019 ◽  
Vol 11 (45) ◽  
pp. 21953-21963 ◽  
Author(s):  
Xiaowei Xu ◽  
Maolei Sun ◽  
Dandan Wang ◽  
Wenhuan Bu ◽  
Zilin Wang ◽  
...  
Keyword(s):  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Silica Nanoparticles ◽  
Bone Morphogenetic Protein ◽  
Porous Silica ◽  
Bone Morphogenetic Protein 2 ◽  
Calcium Deposition ◽  
Morphogenetic Protein

Bone morphogenetic protein-2 plasmid was encapsulated by polyethylenimine-modified porous silica nanoparticles, which promoted osteogenic differentiation and increased calcium deposition with the involvement of autophagy.

scholarly journals Bone Morphogenetic Protein-2 Conjugated to Quantum Dot®s is Biologically Functional

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1208
Author(s):  
Daniel Halloran ◽  
Vrathasha Vrathasha ◽  
Hilary W. Durbano ◽  
Anja Nohe
Keyword(s):  
Quantum Dot ◽  
Bone Morphogenetic Protein ◽  
Fluorescent Dyes ◽  
Fluorescein Isothiocyanate ◽  
Biologically Active ◽  
Bone Morphogenetic Protein 2 ◽  
Bmp Receptors ◽  
Morphogenetic Protein ◽  
Long Time ◽  
Neural Growth

Quantum Dot®s (QDot®s) are novel, semi-conductive nanostructures that emit a certain fluorescence when excited by specific wavelengths. QDot®s are more photostable, brighter, and photobleach less than other fluorescent dyes. These characteristics give them the potential to be used in many biological applications. The shells of QDot®s are coated with functional groups, such as carboxylate and organic groups, allowing them to couple to peptides/proteins and be used for real-time imaging and high-resolution microscopy. Here, we utilize Quantum Dot®s and Bone Morphogenetic Protein-2 (BMP-2) to create a BMP-2-QDot®s conjugate. BMP-2 is a growth factor that drives many processes such as cardiogenesis, neural growth, and osteogenesis. Despite its numerous roles, the trafficking and uptake of BMP-2 into cells is not well-established, especially during progression of diseases. The results presented here demonstrate for the first time a fluorescent BMP-2 analog that binds to the BMP-receptors (BMPRs), remains biologically active, and is stable for long time periods. Previous attempts to develop a biological BMP-2 analog with Fluorescein isothiocyanate (FITC) or nanodiamonds lacked data on the analog’s stability. Furthermore, these analogs did not address whether they can signal within the cell by binding to the BMPRs or were mediated by non-stable conjugates.

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