scholarly journals A proteomics informed by transcriptomics insight into the proteome of Ornithodoros erraticus adult tick saliva

2022 ◽  
Vol 15 (1) ◽  
Author(s):  
Ricardo Pérez-Sánchez ◽  
Angel Carnero-Morán ◽  
M. Luz Valero ◽  
Ana Oleaga
Keyword(s):  
Mass Spectrometry ◽  
Protease Inhibitors ◽  
Model Organism ◽  
African Swine Fever ◽  
Heme Iron ◽  
Reference Database ◽  
Host Feeding ◽  
Tick Feeding ◽  
Tick Saliva ◽  
Proteomics Informed By Transcriptomics

Abstract Background The argasid tick Ornithodoros erraticus is the main vector of tick-borne human relapsing fever (TBRF) and African swine fever (ASF) in the Mediterranean Basin. The prevention and control of these diseases would greatly benefit from the elimination of O. erraticus populations, and anti-tick vaccines are envisaged as an effective and sustainable alternative to chemical acaricide usage for tick control. Ornithodoros erraticus saliva contains bioactive proteins that play essential functions in tick feeding and host defence modulation, which may contribute to host infection by tick-borne pathogens. Hence, these proteins could be candidate antigen targets for the development of vaccines aimed at the control and prevention of O. erraticus infestations and the diseases this tick transmits. The objective of the present work was to obtain and characterise the proteome of the saliva of O. erraticus adult ticks as a means to identify and select novel salivary antigen targets. Methods A proteomics informed by transcriptomics (PIT) approach was applied to analyse samples of female and male saliva separately using the previously obtained O. erraticus sialotranscriptome as a reference database and two different mass spectrometry techniques, namely liquid chromatography–tandem mass spectrometry (LC–MS/MS) in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra MS (SWATH-MS). Results Up to 264 and 263 proteins were identified by LC–MS/MS in the saliva of O. erraticus female and male ticks, respectively, totalling 387 non-redundant proteins. Of these, 224 were further quantified by SWATH-MS in the saliva of both male and female ticks. Quantified proteins were classified into 23 functional categories and their abundance compared between sexes. Heme/iron-binding proteins, protease inhibitors, proteases, lipocalins and immune-related proteins were the categories most abundantly expressed in females, while glycolytic enzymes, protease inhibitors and lipocalins were the most abundantly expressed in males. Ninety-seven proteins were differentially expressed between the sexes, of which 37 and 60 were overexpressed in females and males, respectively. Conclusions The PIT approach demonstrated its usefulness for proteomics studies of O. erraticus, a non-model organism without genomic sequences available, allowing the publication of the first comprehensive proteome of the saliva of O. erraticus reported to date. These findings confirm important quantitative differences between sexes in the O. erraticus saliva proteome, unveil novel salivary proteins and functions at the tick–host feeding interface and improve our understanding of the physiology of feeding in O. erraticus ticks. The integration of O. erraticus sialoproteomic and sialotranscriptomic data will drive a more rational selection of salivary candidates as antigen targets for the development of vaccines aimed at the control of O. erraticus infestations and the diseases it transmits. Graphical Abstract

scholarly journals A Proteomics Informed by Transcriptomics Insight Into the Proteome of Ornithodoros Erraticus Adult Tick Saliva

2021 ◽  
Author(s):  
Ricardo Pérez-Sánchez ◽  
Angel Carnero-Morán ◽  
M. Luz Valero ◽  
Ana Oleaga
Keyword(s):  
Mass Spectrometry ◽  
Protease Inhibitors ◽  
Model Organism ◽  
African Swine Fever ◽  
Heme Iron ◽  
Reference Database ◽  
Host Feeding ◽  
Tick Feeding ◽  
Tick Saliva ◽  
Proteomics Informed By Transcriptomics

Abstract Background: The argasid tick Ornithodoros erraticus is the main vector of tick-borne human relapsing fever (TBRF) and African swine fever (ASF) in the Mediterranean Basin. The prevention and control of these diseases would greatly benefit from the elimination of O. erraticus populations, and anti-tick vaccines are envisaged as an effective and sustainable alternative to chemical acaricide usage for tick control. O. erraticus saliva contains bioactive proteins that play essential functions for tick feeding and host defence modulation, which may contribute to host infection by tick-borne pathogens. Hence, these proteins could be candidate antigen targets for the development of vaccines aimed at the control and prevention of O. erraticus infestations and the diseases it transmits. The objective of the present work was to obtain and characterise the proteome of the saliva of O. erraticus adult ticks as a means to identify and select novel salivary antigen targets.Methods: We have applied a proteomics informed by transcriptomics (PIT) approach to analyse samples of female and male saliva separately using the previously obtained O. erraticus sialotranscriptome as a reference database, and two different mass spectrometry techniques, namely, LC-MS/MS in data-dependent acquisition mode and sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). Results: Up to 264 and 263 proteins were identified by LC-MS/MS in the saliva of O. erraticus female and male ticks, respectively, totalling 387 non-redundant proteins. Of them, 224 were further quantified by SWATH-MS in both male and female saliva. Quantified proteins were classified into 23 functional categories and their abundance compared between sexes. Heme/iron binding proteins, protease inhibitors, proteases, lipocalins and immune-related proteins were the categories most abundantly expressed in females, while glycolytic enzymes, protease inhibitors and lipocalins were the most abundantly expressed in males. Ninety-seven proteins were differentially expressed between the sexes: 37 were overexpressed in females and 60 in males. Conclusions: The PIT approach demonstrated its usefulness for proteomics studies of O. erraticus, a non-model organism without genomic sequences available, allowing the publication of the first comprehensive proteome of the saliva of O. erraticus reported to date. These findings confirm important quantitative differences between sexes in the O. erraticus saliva proteome, unveil novel salivary proteins and functions at the tick–host feeding interface and help to understand the physiology of feeding in O. erraticus ticks. The integration of O. erraticus sialoproteomic and sialotranscriptomic data will drive a more rational selection of salivary candidates as antigen targets for the development of vaccines aimed at the control of O. erraticus infestations and the diseases it transmits.

scholarly journals Proteomics Informed by Transcriptomics for a Qualitative and Quantitative Analysis of the Sialoproteome of Ornithodoros Moubata Adult Ticks

2021 ◽  
Author(s):  
Ana Oleaga ◽  
Angel Carnero-Moran ◽  
M. Luz Valero ◽  
Ricardo Pérez-Sánchez
Keyword(s):  
Mass Spectrometry ◽  
Vaccine Development ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Host Feeding ◽  
Tick Saliva ◽  
Ornithodoros Moubata ◽  
Tick Vaccine ◽  
Proteomics Informed By Transcriptomics ◽  
Selection Of

Abstract Background The argasid tick Ornithodoros moubata is the main vector in mainland Africa of the African swine fever virus and the spirochete Borrelia duttoni, which causes human relapsing fever. Elimination of O. moubata populations would contribute to the prevention and control of these two severe diseases. The development of anti-tick vaccines is an eco-friendly and sustainable method for the elimination of tick populations. The tick saliva forms part of the tick-host interface and knowing its composition is key for the identification and selection of vaccine candidate antigens. The aim of the present work is to expand the data on the saliva proteome composition of O. moubata adult ticks, particularly of female ticks, since a more in-depth knowledge of the O. moubata sialome will allow identifying and selecting novel salivary antigens as targets for tick vaccines. Methods We have analysed samples of female and male saliva using two different mass spectrometry approaches: data-dependent acquisition LC-MS/MS and sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS). To maximise the number of protein identifications, a proteomics informed by transcriptomics (PIT) analysis was applied using the O. moubata salivary transcriptomic dataset previously obtained by RNAseq. Results The SWATH-MS proved to be superior to LC-MS/MS in the study of female saliva since it increased by 60% the number of identified proteins, enhanced the reproducibility of the results and provided a quantitative image of the saliva components. As a whole, we have identified 299 non-redundant proteins in the O. moubata saliva and quantified the expression of 165 of them in both male and female saliva, among which 13 were significantly overexpressed in females and 40 in males. These results evidence important quantitative differences between sexes in the saliva proteome. Conclusions This work expand our knowledge of the O. moubata sialome, particularly of female ticks, by increasing the identification of novel salivary proteins and functions at the tick–host feeding interface. The integration of this new knowledge together with the information from the O. moubata sialotranscriptome will allow a more rational selection of the salivary candidates as antigen targets for tick vaccine development.

scholarly journals Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of adult Ornithodoros moubata ticks

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ana Oleaga ◽  
Angel Carnero-Morán ◽  
M. Luz Valero ◽  
Ricardo Pérez-Sánchez
Keyword(s):  
Vaccine Development ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
The Body ◽  
Host Feeding ◽  
Tick Saliva ◽  
Ornithodoros Moubata ◽  
Tick Vaccine ◽  
Proteomics Informed By Transcriptomics ◽  
Selection Of

Abstract Background The argasid tick Ornithodoros moubata is the main vector in mainland Africa of African swine fever virus and the spirochete Borrelia duttoni, which causes human relapsing fever. The elimination of populations of O. moubata would contribute to the prevention and control of these two serious diseases. Anti-tick vaccines are an eco-friendly and sustainable means of eliminating tick populations. Tick saliva forms part of the tick-host interface, and knowledge of its composition is key to the identification and selection of vaccine candidate antigens. The aim of the present work is to increase the body of data on the composition of the saliva proteome of adult O. moubata ticks, particularly of females, since in-depth knowledge of the O. moubata sialome will allow the identification and selection of novel salivary antigens as targets for tick vaccines. Methods We analysed samples of female and male saliva using two different mass spectrometry (MS) approaches: data-dependent acquisition liquid chromatography-tandem MS (LC–MS/MS) and sequential window acquisition of all theoretical fragment ion spectra–MS (SWATH-MS). To maximise the number of proteins identified, a proteomics informed by transcriptomics analysis was applied using the O. moubata salivary transcriptomic dataset previously obtained by RNA-Seq. Results SWATH-MS proved to be superior to LC–MS/MS for the study of female saliva, since it identified 61.2% more proteins than the latter, the reproducibility of results was enhanced with its use, and it provided a quantitative picture of salivary components. In total, we identified 299 non-redundant proteins in the saliva of O. moubata, and quantified the expression of 165 of these in both male and female saliva, among which 13 were significantly overexpressed in females and 40 in males. These results indicate important quantitative differences in the saliva proteome between the sexes. Conclusions This work expands our knowledge of the O. moubata sialome, particularly that of females, by increasing the number of identified novel salivary proteins, which have different functions at the tick–host feeding interface. This new knowledge taken together with information on the O. moubata sialotranscriptome will allow a more rational selection of salivary candidates as antigen targets for tick vaccine development. Graphical Abstract

foodMASST: A Mass Spectrometry Search Tool for Foods and Beverages.

2021 ◽  
Author(s):  
Pieter Dorrestein ◽  
Kiana West ◽  
Robin Schmid ◽  
Julia Gauglitz ◽  
Mingxun Wang
Keyword(s):  
Mass Spectrometry ◽  
Scientific Community ◽  
Biomarker Discovery ◽  
Reference Database ◽  
Chemical Complexity ◽  
Nutrition Research ◽  
Food And Beverage ◽  
Search Tool ◽  
Insight Into

Abstract There is a growing interest in unraveling the chemical complexity of our diets. To help the scientific community gain insight into the molecules present in foods and beverages that we ingest, we created foodMASST, a search tool for MS/MS spectra (of both known and unknown molecules) against a growing metabolomics food and beverage reference database. We envision foodMASST will become valuable for nutrition research and dietary biomarker discovery.

scholarly journals A mass spectrometry guided approach for the identification of novel vaccine candidates in gram-negative pathogens

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Daniel Hornburg ◽  
Tobias Kruse ◽  
Florian Anderl ◽  
Christina Daschkin ◽  
Raphaela P. Semper ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Model Organism ◽  
Antibody Responses ◽  
Surface Proteins ◽  
Gram Negative ◽  
Vaccine Candidates ◽  
Bacterial Proteins ◽  
Computational Homology ◽  
Cell Responses ◽  
Homology Inference

AbstractVaccination is the most effective method to prevent infectious diseases. However, approaches to identify novel vaccine candidates are commonly laborious and protracted. While surface proteins are suitable vaccine candidates and can elicit antibacterial antibody responses, systematic approaches to define surfomes from gram-negatives have rarely been successful. Here we developed a combined discovery-driven mass spectrometry and computational strategy to identify bacterial vaccine candidates and validate their immunogenicity using a highly prevalent gram-negative pathogen, Helicobacter pylori, as a model organism. We efficiently isolated surface antigens by enzymatic cleavage, with a design of experiment based strategy to experimentally dissect cell surface-exposed from cytosolic proteins. From a total of 1,153 quantified bacterial proteins, we thereby identified 72 surface exposed antigens and further prioritized candidates by computational homology inference within and across species. We next tested candidate-specific immune responses. All candidates were recognized in sera from infected patients, and readily induced antibody responses after vaccination of mice. The candidate jhp_0775 induced specific B and T cell responses and significantly reduced colonization levels in mouse therapeutic vaccination studies. In infected humans, we further show that jhp_0775 is immunogenic and activates IFNγ secretion from peripheral CD4+ and CD8+ T cells. Our strategy provides a generic preclinical screening, selection and validation process for novel vaccine candidates against gram-negative bacteria, which could be employed to other gram-negative pathogens.

Identification of phlebotomine sand flies through MALDI-TOF mass spectrometry and in-house reference database

Acta Tropica ◽  
2019 ◽  
Vol 194 ◽  
pp. 47-52 ◽  
Author(s):  
Francesca Arfuso ◽  
Gabriella Gaglio ◽  
Jessica Maria Abbate ◽  
Giulia Caracappa ◽  
Angelo Lupia ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Reference Database ◽  
Sand Flies ◽  
Phlebotomine Sand Flies ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Capillary electrophoresis coupled to MALDI mass spectrometry imaging with large volume sample stacking injection for improved coverage of C. borealis neuropeptidome

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Kellen DeLaney ◽  
Lingjun Li
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Mass Spectrometry Imaging ◽  
Model Organism ◽  
Laser Desorption ◽  
Maldi Mass Spectrometry ◽  
Laser Desorption Ionization ◽  
Cancer Borealis ◽  
Sample Stacking ◽  
Ms Imaging

This work reports on a capillary electrophoresis (CE) separation method coupled to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) imaging for improved neuropeptide coverage in the model organism Cancer borealis.

scholarly journals The Immunomodulatory Effect of IrSPI, a Tick Salivary Gland Serine Protease Inhibitor Involved in Ixodes ricinus Tick Feeding

Vaccines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 148 ◽  
Author(s):  
Adrien Blisnick ◽  
Ladislav Šimo ◽  
Catherine Grillon ◽  
Fabienne Fasani ◽  
Sébastien Brûlé ◽  
...  
Keyword(s):  
Protease Inhibitor ◽  
Serine Protease ◽  
Ixodes Ricinus ◽  
Serine Protease Inhibitor ◽  
Blood Feeding ◽  
Immunomodulatory Activity ◽  
Hard Tick ◽  
Tick Feeding ◽  
Tick Saliva ◽  
Elastase Inhibitor

Ticks are the most important vectors of pathogens affecting both domestic and wild animals worldwide. Hard tick feeding is a slow process—taking up to several days—and necessitates extended control over the host response. The success of the feeding process depends upon injection of tick saliva, which not only controls host hemostasis and wound healing, but also subverts the host immune response to avoid tick rejection that creates a favorable niche for the survival and propagation of diverse tick-borne pathogens. Here, we report on the molecular and biochemical features and functions of an Ixodes ricinus serine protease inhibitor (IrSPI). We characterize IrSPI as a Kunitz elastase inhibitor that is overexpressed in several tick organs—especially salivary glands—during blood-feeding. We also demonstrated that when IrSPI is injected into the host through saliva, it had no impact on tissue factor pathway-induced coagulation, fibrinolysis, endothelial cell angiogenesis or apoptosis, but the protein exhibits immunomodulatory activity. In particular, IrSPI represses proliferation of CD4+ T lymphocytes and proinflammatory cytokine secretion from both splenocytes and macrophages. Our study contributes valuable knowledge to tick-host interactions and provides insights that could be further exploited to design anti-tick vaccines targeting this immunomodulator implicated in I. ricinus feeding.

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