scholarly journals Non-invasive detection of endometrial cancer by DNA methylation analysis in urine

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Rianne van den Helder ◽  
Birgit M. M. Wever ◽  
Nienke E. van Trommel ◽  
Annina P. van Splunter ◽  
Constantijne H. Mom ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Endometrial Cancer ◽  
Cancer Detection ◽  
Characteristic Curve ◽  
Sample Type ◽  
Methylation Analysis ◽  
Urine Sediment ◽  
Non Invasive ◽  
Diagnostic Potential ◽  
Dna Methylation Analysis

Abstract Background The incidence of endometrial cancer is rising, and current diagnostics often require invasive biopsy procedures. Urine may offer an alternative sample type, which is easily accessible and allows repetitive self-sampling at home. Here, we set out to investigate the feasibility of endometrial cancer detection in urine using DNA methylation analysis. Results Urine samples of endometrial cancer patients (n = 42) and healthy controls (n = 46) were separated into three fractions (full void urine, urine sediment, and urine supernatant) and tested for three DNA methylation markers (GHSR, SST, ZIC1). Strong to very strong correlations (r = 0.77–0.92) were found amongst the different urine fractions. All DNA methylation markers showed increased methylation levels in patients as compared to controls, in all urine fractions. The highest diagnostic potential for endometrial cancer detection in urine was found in full void urine, with area under the receiver operating characteristic curve values ranging from 0.86 to 0.95. Conclusions This feasibility study demonstrates, for the first time, that DNA methylation analysis in urine could provide a non-invasive alternative for the detection of endometrial cancer. Further investigation is warranted to validate its clinical usefulness. Potential applications of this diagnostic approach include the screening of asymptomatic women, triaging women with postmenopausal bleeding symptoms, and monitoring women with increased endometrial cancer risk.

scholarly journals 13-gene DNA Methylation Analysis from Oral Brushing: A Promising Non Invasive Tool in the Follow-up of Oral Cancer Patients

2019 ◽  
Vol 8 (12) ◽  
pp. 2107 ◽  
Author(s):  
Davide B. Gissi ◽  
Achille Tarsitano ◽  
Andrea Gabusi ◽  
Roberto Rossi ◽  
Giuseppe Attardo ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Oral Cancer ◽  
Cancer Treatment ◽  
Sample Collection ◽  
Methylation Analysis ◽  
Non Invasive ◽  
Locoregional Relapse ◽  
Positive Score ◽  
Dna Methylation Analysis

Background: This study aimed to evaluate the prognostic value of a non-invasive sampling procedure based on 13-gene DNA methylation analysis in the follow-up of patients previously treated for oral squamous cell carcinoma (OSCC). Methods: The study population included 49 consecutive patients treated for OSCC. Oral brushing sample collection was performed at two different times: before any cancer treatment in the tumor mass and during patient follow-up almost 6 months after OSCC treatment, within the regenerative area after OSCC resection. Each sample was considered positive or negative in relation to a predefined cut-off value. Results: Before any cancer treatment, 47/49 specimens exceeded the score and were considered as positive. Six months after OSCC resection, 16/49 specimens also had positive scores in the samples collected from the regenerative area. During the follow-up period, 7/49 patients developed locoregional relapse: 6/7 patients had a positive score in the regenerative area after OSCC resection. The presence of a positive score after oral cancer treatment was the most powerful variable related to the appearance of locoregional relapse. Conclusion: 13-gene DNA methylation analysis by oral brushing may have a clinical application as a prognostic non-invasive tool in the follow-up of patients surgically treated for OSCC.

scholarly journals Cervical cancer detection by DNA methylation analysis in urine

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Barbara C. Snoek ◽  
Annina P. van Splunter ◽  
Maaike C. G. Bleeker ◽  
Maartje C. van Ruiten ◽  
Daniëlle A. M. Heideman ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Methylation ◽  
Cancer Detection ◽  
Methylation Analysis ◽  
Dna Methylation Analysis

scholarly journals CADM1, MAL and miR124-2 methylation analysis in cervical scrapes to detect cervical and endometrial cancer

2014 ◽  
Vol 67 (12) ◽  
pp. 1067-1071 ◽  
Author(s):  
Lise M A De Strooper ◽  
Marjolein van Zummeren ◽  
Renske D M Steenbergen ◽  
Maaike C G Bleeker ◽  
Albertus T Hesselink ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Methylation ◽  
Endometrial Cancer ◽  
Gene Promoter ◽  
Intraepithelial Neoplasia ◽  
Promoter Hypermethylation ◽  
Methylation Analysis ◽  
Cervical Intraepithelial Neoplasia Grade ◽  
Specific Pcr ◽  
Dna Methylation Analysis

AimsGene promoter hypermethylation is recognised as an essential early step in carcinogenesis, indicating important application areas for DNA methylation analysis in early cancer detection. The current study was set out to assess the performance of CADM1, MAL and miR124-2 methylation analysis in cervical scrapes for detection of cervical and endometrial cancer.MethodsA series of cervical scrapes of women with cervical (n=79) or endometrial (n=21) cancer, cervical intraepithelial neoplasia grade 3 (CIN3) (n=16) or CIN2 (n=32), and women without evidence of CIN2 or worse (n=120) were assessed for methylation of CADM1, MAL and miR124-2. Methylation analysis was done by the PreCursor-M assay, a multiplex quantitative methylation-specific PCR.ResultsAll samples of women with cervical cancer (79/79, 100%), independent of the histotype, and 76% (16/21; 95% CI 58.0% to 94.4%) of women with endometrial cancer scored positive for DNA methylation for at least one of the three genes. In women without cancer, methylation frequencies increased significantly with severity of disease from 19.2% (23/120; 95% CI 12.1% to 26.2%) in women without CIN2 or worse to 37.5% (12/32; 95% CI 20.7% to 54.3%) and 68.8% (11/16; 95% CI 46.0% to 91.5%) in women with CIN2 and CIN3, respectively. Overall methylation positivity and the number of methylated genes increased proportionally to the lesion severity.ConclusionsDNA methylation analysis of CADM1, MAL and miR124-2 in cervical scrapes consistently detects cervical cancer and the majority of CIN3 lesions, and has the capacity to broaden its use on cervical scrapes through the detection of a substantial subset of endometrial carcinomas.

scholarly journals Genome-wide DNA methylation analysis in obese women predicts an epigenetic signature for future endometrial cancer

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Masaru Nagashima ◽  
Naofumi Miwa ◽  
Hajime Hirasawa ◽  
Yukiko Katagiri ◽  
Ken Takamatsu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Endometrial Cancer ◽  
Obese Women ◽  
Methylation Analysis ◽  
Genome Wide ◽  
Dna Methylation Analysis

scholarly journals 13-Gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Diagnostic Tool in the Follow-Up of Patients Surgically Treated for Oral Cancer

Proceedings ◽  
2019 ◽  
Vol 35 (1) ◽  
pp. 32
Author(s):  
Morselli ◽  
Rossi ◽  
Morandi ◽  
Tarsitano ◽  
Gabusi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Diagnostic Tool ◽  
Significant Risk ◽  
Methylation Analysis ◽  
Non Invasive ◽  
Dna Methylation Analysis

Patients treated for Oral Squamous Cell Carcinoma (OSCC) showed a significant risk todevelop a loco-regional relapse during follow-up period. [...]

scholarly journals Clinical Validation of 13-gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Sampling Procedure for Early Detection of Oral Squamous Cell Carcinoma. A Multicentric Study

Proceedings ◽  
2019 ◽  
Vol 35 (1) ◽  
pp. 27
Author(s):  
Gissi ◽  
Romeo ◽  
Tenore ◽  
Pentenero ◽  
Campisi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Early Detection ◽  
Sampling Procedure ◽  
Clinical Validation ◽  
Methylation Analysis ◽  
Multicentric Study ◽  
Non Invasive ◽  
Dna Methylation Analysis

In a recent study our research group described a non-invasive sampling procedure based on [...]

scholarly journals Detection of colorectal cancer in urine using DNA methylation analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
S. Bach ◽  
I. Paulis ◽  
N. R. Sluiter ◽  
M. Tibbesma ◽  
I. Martin ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Regression Tree ◽  
Classification And Regression Tree ◽  
Multivariate Logistic Regression ◽  
Methylation Analysis ◽  
Methylated Dna ◽  
Non Invasive ◽  
Regression Tree Analysis ◽  
Dna Methylation Analysis

AbstractColorectal cancer (CRC) is the second leading cause for cancer-related death globally. Clinically, there is an urgent need for non-invasive CRC detection. This study assessed the feasibility of CRC detection by analysis of tumor-derived methylated DNA fragments in urine. Urine samples, including both unfractioned and supernatant urine fractions, of 92 CRC patients and 63 healthy volunteers were analyzed for DNA methylation levels of 6 CRC-associated markers (SEPT9, TMEFF2, SDC2, NDRG4, VIM and ALX4). Optimal marker panels were determined by two statistical approaches. Methylation levels of SEPT9 were significantly increased in urine supernatant of CRC patients compared to controls (p < 0.0001). Methylation analysis in unfractioned urine appeared inaccurate. Following multivariate logistic regression and classification and regression tree analysis, a marker panel consisting of SEPT9 and SDC2 was able to detect up to 70% of CRC cases in urine supernatant at 86% specificity. First evidence is provided for CRC detection in urine by SEPT9 methylation analysis, which combined with SDC2 allows for an optimal differentiation between CRC patients and controls. Urine therefore provides a promising liquid biopsy for non-invasive CRC detection.

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