Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats

Objective: This is a systematic review of the effects of low-intensity pulsed ultrasound (LIPUS) on stem cell differentiation. Background Data: Recent studies have investigated several types of stem cells from different sources in the body. These stem cells should strictly be certified and promoted for cell therapies before being used in medical applications. LIPUS has been used extensively in treatment centers and in research to promote stem cell differentiation, function, and proliferation. Materials and Methods: The databases of PubMed, Google Scholar, and Scopus were searched for abstracts and full-text scientific papers published from 1989-2019 that reported the application of LIPUS on stem cell differentiation. Related English language articles were found using the following defined keywords: low-intensity pulsed ultrasound, stem cell, differentiation. Criteria for inclusion in the review were: LIPUS with frequencies of 1–3 MHz and pulsed ultrasound intensity of <500 mW/cm2. Duration, exposure time, and cell sources were taken into consideration. Results: Fifty-two articles were selected based on the inclusion criteria. Most articles demonstrated that the application of LIPUS had positive effects on stem cell differentiation. However, some authors recommended that LIPUS combined with other physical therapy aides was more effective in stem cell differentiation. Conclusions: LIPUS significantly increases the level of stem cell differentiation in cells derived mainly from bone marrow mesenchymal stem cells. There is a need for further studies to analyze the effect of LIPUS on cells derived from other sources, particularly adipose tissue-derived mesenchymal stem cells, for treating hard diseases, such as osteoporosis and diabetic foot ulcer. Due to a lack of reporting on standard LIPUS parameters in the field, more experiments comparing the protocols for standardization of LIPUS parameters are needed to establish the best protocol, which would allow for the best results.

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Therapeutic Effects of Low-Intensity Pulsed Ultrasound on Premature Ovarian Insufficiency

Ultrasound in Medicine & Biology ◽

10.1016/j.ultrasmedbio.2021.04.022 ◽

2021 ◽

Author(s):

Huajun Tang ◽

Yao Liu ◽

Yijin Fan ◽

Chengzhi Li

Keyword(s):

Premature Ovarian Insufficiency ◽

Therapeutic Effects ◽

Pulsed Ultrasound ◽

Ovarian Insufficiency ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity

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Low-intensity pulsed ultrasound promotes repair of 4-vinylcyclohexene diepoxide induced premature ovarian insufficiency in SD rats

The Journals of Gerontology Series A ◽

10.1093/gerona/glab242 ◽

2021 ◽

Author(s):

Juan Qin ◽

Junlin Chen ◽

Haopeng Xu ◽

Yi Xia ◽

Wentao Tang ◽

...

Keyword(s):

Estrous Cycle ◽

Premature Ovarian Insufficiency ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Pulsed Ultrasound ◽

Ovarian Insufficiency ◽

Vinylcyclohexene Diepoxide ◽

Low Intensity Pulsed Ultrasound ◽

Low Intensity ◽

Sd Rats

Abstract Women with premature ovarian insufficiency (POI) may be more vulnerable to a variety of health risks. To seek a new method to treat the disease, the effects of low intensity pulsed ultrasound (LIPUS) on promoting repair of ovarian injury in female SD rats induced by 4-vinylcyclohexene diepoxide (VCD) were explored in this research. A total of 24 female SD rats were subjected to intraperitoneal injection of VCD to induce POI. Successful modeling was achieved in 22 rats, which were then randomized into VCD + LIPUS group (n=13) and VCD group (n=9). The control group (n=5) was injected with equal normal saline. Hematoxylin and eosin staining (H&E staining), enzyme-linked immunosorbent assay (ELISA), western-blot analysis, scanning electron microscope (SEM), immunohistochemistry (IHC) and terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay were applied to detect the results. The results indicated that rats in the VCD group showed disorder in the estrous cycle, the number of atresia follicles and apoptosis granulosa cells increased (P < 0.05). After the LIPUS treatment, the estrous cycle recovered, the number of follicles increased (P < 0.05), the level of E2 and AMH enhanced (P < 0.05) and the FSH decreased (P < 0.05). The expression of NF-κB p65, TNFα, Bax, ATF4 and caspase-3 in ovarian tissue significantly decreased (P < 0.05). These findings showed that LIPUS could promote the repair of the VCD induced ovarian damage in SD rats, which has the potential to be further applied in the clinic.

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