Low-intensity pulsed ultrasound promotes repair of 4-vinylcyclohexene diepoxide induced premature ovarian insufficiency in SD rats

2021 ◽  
Author(s):  
Juan Qin ◽  
Junlin Chen ◽  
Haopeng Xu ◽  
Yi Xia ◽  
Wentao Tang ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Premature Ovarian Insufficiency ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Ovarian Insufficiency ◽  
Vinylcyclohexene Diepoxide ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Sd Rats

Abstract Women with premature ovarian insufficiency (POI) may be more vulnerable to a variety of health risks. To seek a new method to treat the disease, the effects of low intensity pulsed ultrasound (LIPUS) on promoting repair of ovarian injury in female SD rats induced by 4-vinylcyclohexene diepoxide (VCD) were explored in this research. A total of 24 female SD rats were subjected to intraperitoneal injection of VCD to induce POI. Successful modeling was achieved in 22 rats, which were then randomized into VCD + LIPUS group (n=13) and VCD group (n=9). The control group (n=5) was injected with equal normal saline. Hematoxylin and eosin staining (H&E staining), enzyme-linked immunosorbent assay (ELISA), western-blot analysis, scanning electron microscope (SEM), immunohistochemistry (IHC) and terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay were applied to detect the results. The results indicated that rats in the VCD group showed disorder in the estrous cycle, the number of atresia follicles and apoptosis granulosa cells increased (P < 0.05). After the LIPUS treatment, the estrous cycle recovered, the number of follicles increased (P < 0.05), the level of E2 and AMH enhanced (P < 0.05) and the FSH decreased (P < 0.05). The expression of NF-κB p65, TNFα, Bax, ATF4 and caspase-3 in ovarian tissue significantly decreased (P < 0.05). These findings showed that LIPUS could promote the repair of the VCD induced ovarian damage in SD rats, which has the potential to be further applied in the clinic.

scholarly journals Effects of Low Intensity Pulsed Ultrasound on Expression of Bax and Bcl-2 in Premature Ovarian Failure Mice Induced by 4-Vinylcyclohexene Diepoxide

2021 ◽  
Author(s):  
Haopeng Xu ◽  
Yi Xia ◽  
Juan Qin ◽  
Jie Xu ◽  
Chongyan Li ◽  
...  
Keyword(s):  
Protein Expression ◽  
Ovarian Failure ◽  
Control Group ◽  
Common Disease ◽  
Follicular Atresia ◽  
Pulsed Ultrasound ◽  
Vinylcyclohexene Diepoxide ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
And Control

Abstract BackgroundPremature ovarian failure (POF) is a common disease in the field of gynecological. This study was performed to determine the effects of low intensity pulsed ultrasound (LIPUS) on granulosa cells (GCs) apoptosis and protein expression of Bax and Bcl-2 in 4-vinylcyclohexene diepoxide (VCD)-induced POF mice. The aim of this research is to investigate the mechanisms of LIPUS on ovarian function and reserve capacity.MethodsThe current POF mice model was administrated with VCD (160 mg/kg) by intraperitoneal injection for 15 consecutive days. The mice were divided into POF group, LIPUS group and control group. In the LIPUS group, the right ovary of mice was treated by LIPUS (acoustic intensity was 200 mW/cm2, frequency was 0.3 MHz, and duty cycle was 20%) for 20 minutes, 15 consecutive days from day 16. The mice of the POF group and control group were treated without ultrasonic output. The basic observation, Hematoxylin and eosin staining (H&E staining), TUNEL assay and immunohistochemistry were applied to detect the results.ResultsThe results showed that VCD can induce estrus cycle disorder, follicular atresia and GCs apoptosis in mice to establish POF model successfully. LIPUS significantly promoted follicular development, inhibited excessive follicular atresia and GCs apoptosis. The mechanism may be achieved by increasing the protein expression of Bcl-2 and decreasing the expression of Bax in ovaries.ConclusionsThese findings have the potential to provide novel methodological foundation for the future research, which help treat POF patients in the clinic.

scholarly journals Histological and Radiological Evaluation of Low-Intensity Pulsed Ultrasound Versus Whole Body Vibration on Healing of Mandibular Bone Defects in Rats

Medicina ◽  
2020 ◽  
Vol 56 (9) ◽  
pp. 457
Author(s):  
Milad Etemadi Sh ◽  
Nan-Chen Hsieh ◽  
Seyed Shahin Movahed Mohammadi ◽  
Shahrooz Momeni ◽  
Seyed Mohammad Razavi ◽  
...  
Keyword(s):  
Bone Density ◽  
Whole Body Vibration ◽  
Bone Defects ◽  
Whole Body ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Mandibular Bone ◽  
Body Vibration ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Background and Objectives: Mechanical stimulation can improve the structural properties of the fracture site and induce the differentiation of different cell types for bone regeneration. This study aimed to compare the effect of low-intensity pulsed ultrasound stimulation (LIPUS) versus whole body vibration (WBV) on healing of mandibular bone defects. Materials and Methods: A mandibular defect was created in 66 rats. The rats were randomly divided into two groups of rats. Each group was subdivided randomly by three groups (n = 11) as follows: (I) control group, (II) treatment with LIPUS, and (III) treatment with WBV. The radiographic changes in bone density, the ratio of lamellar bone to the entire bone volume, the ratio of the newly formed bone to the connective tissue and inflammation grade were evaluated after 1 and 2 months. Results: LIPUS significantly increased the radiographic bone density change compared to the control group at the first and second month postoperatively (p < 0.01). WBV only significantly increased the bone density compared to the control group at the second month after the surgery (p < 0.01). Conclusions: Application of LIPUS and WBV may enhance the regeneration of mandibular bone defects in rats. Although LIPUS and WBV are effective in mandibular bone healing, the effects of LIPUS are faster and greater than WBV.

scholarly journals Effects of low intensity pulsed ultrasound to reduce the effusion volumes and pain with knee osteoarthritis: a randomized controlled trial

2018 ◽  
Vol 5 (1) ◽  
pp. 77
Author(s):  
P. Shanmuga Raju ◽  
Chokkarapu Ramu ◽  
N. S. Harshavardhan ◽  
K. Rajender ◽  
G. Sachin
Keyword(s):  
Randomized Controlled Trial ◽  
Knee Osteoarthritis ◽  
Controlled Trial ◽  
Control Group ◽  
Ultrasound Therapy ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Randomized Controlled ◽  
And Control

<p class="abstract"><strong>Background:</strong> The study aim was to determine the effect of low intensity pulsed ultrasound therapy to reduce the effusion volumes and pain in patients with Knee OA.</p><p class="abstract"><strong>Methods:</strong> This study design was randomized controlled trial. Total 50 patients diagnosed with Knee osteoarthritis were randomly assigned to two groups. Group I was using treatment of low intensity pulsed ultrasound therapy and group II was administered TENS with home exercise respectively. Treatments were 6 days per week and duration of 2 week. The amount of effusion volume will be measured via ultrasonograpy in knee.<strong></strong></p><p class="abstract"><strong>Results:</strong> The<strong> </strong>maximum number of cases are lying in age group &gt;60 years which is 28% and 40% in cases and control group respectively and age distribution in both the group is statistically not significant. The mean age of patients in cases and control group is 57.08±7.40 years and 58.04±9.93 years respectively.</p><p class="abstract"><strong>Conclusions:</strong> Low intensity pulsed ultrasound therapy significantly reduced the effusion volumes and pain in patients with knee osteoarthritis.</p>

scholarly journals Evaluation of histological changes in peri-implant bone tissue after ultrasound application at early healing stages

2018 ◽  
pp. 38-42 ◽  
Author(s):  
S.P. Rubnikovich ◽  
I.S. Khomich
Keyword(s):  
Bone Tissue ◽  
Dental Implants ◽  
Morphological Changes ◽  
Experimental Studies ◽  
Low Frequency ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Dental Implantation

The aim was to study the nature of the morphological changes over time in the bone tissue around the implants under the influence of low-intensity pulsed ultrasound in the experiment. Materials and methods. Experimental studies were carried out on rabbits of the chinchilla breed, with dental implants installed in the tibia – two experimental groups with ultrasound and one control group. The animals were withdrawn from the experiment at 1, 2, 4 and 8 weeks and histological examination of the sections of peri-implant tissues was carried out. Results. The processes of osseointegration of dental implants in all groups of animals occurred without disrupting the normal process sequence. In the early stages granulation tissue is formed, later it is replaced by fibrous-reticular and coarse-fibrous bone tissue, and at the end – by a more mature lamellar bone tissue. However, the timing and degree of maturation of bone tissue, as well as osseointegration of implants in groups using low-frequency low-intensity pulsed ultrasound and without it differed significantly. The study showed that ultrasound exposure to implants and surrounding tissues induces osteoreparation processes, stimulating neoangiogenesis in granulation and newly formed bone tissue. Conclusion. Application of ultrasound to implants and subsequent application to the peri-implant tissues during and after dental implantation promotes the formation of bone tissue, identical by the histostructure to the maternal bone.

A influência da irradiação ultrassônica de baixa intensidade em cultura de células fibroblásticas

2015 ◽  
Vol 13 ◽  
pp. 306
Author(s):  
Larissa Dragonetti Bertin ◽  
Deise Aparecida Almeida Pires-Oliveira ◽  
Priscila Daniela Oliveira ◽  
Juliana Almeida Serpeloni ◽  
Stheace Kelly Fernandes Szezerbaty ◽  
...  
Keyword(s):  
Duty Cycle ◽  
Healing Process ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Fibroblast Cells ◽  
Pulse Regime ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Introduction: In vitro and in vivo put in evidence that the Low Intensity Pulsed Ultrasound therapy exerts a significant influence on cell function (cytoskeleton organization, stimulation of mitochondrial activity, ATP levels and plasma membrane). Objective: This study will analyze the radiation of low intensity pulsed ultrasound in fibroblast cells L 929. Method: In this study are presented the data from each exposure group average and standard deviation in each moment of evaluation (24 hours, 48 hours and 72 hours). The control group (received no radiation), 0.2 W/cm2 with 10% pulse regime (1: 9 duty cycle), 0.2 W / cm2 with 20% pulse regime (2: 8 cycle work), 0.4 W/cm2 with pulse scheme 10% (1: 9 duty cycle), 0.4 W/cm2 with pulse scheme 20% (2: 8 duty cycle). The analyzes will be performed through optical microscopy, MTT method 3 - (4,5-dimethylthiazol-2-yl) -2,5 diphenyl tetrazolium bromide, within the incubation times of 24, 48 and 72 hours. Results: Given the above study, the results presented in this project will be directed to increase the stimulation process and proliferation of fibroblast cells from the pulsed ultrasonic irradiation of low intensity, correlating with the healing process, neovascularization and repair. Conclusion: Therefore, the study of the effect of ultrasound from cell culture provides us with a simple and informative model on the significant aspects of the use of physical therapy in vivo system.

Low-Intensity Pulsed Ultrasound Alleviates Osteoarthritis Condition Through Focal Adhesion Kinase–Mediated Chondrocyte Proliferation and Differentiation

Cartilage ◽  
2020 ◽  
pp. 194760352091232 ◽  
Author(s):  
Fei Sang ◽  
Jin Xu ◽  
Zheng Chen ◽  
Qingbai Liu ◽  
Wenchao Jiang
Keyword(s):  
Synovial Fluid ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pulsed Ultrasound ◽  
Chondrocyte Proliferation ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Tnf Α ◽  
Proliferation And Differentiation

Objective Osteoarthritis (OA) is a prevalent chronic multifactorial degenerative disease characterized by joint tissue inflammation, osteophyte formation, subchondral bone sclerosis, and articular cartilage degradation. Low-intensity pulsed ultrasound (LIPUS), a noninvasive ultrasound technique, is widely used to attenuate diseases. The aim of this study was to investigate whether LIPUS can ameliorate OA, and to explore its underlying molecular mechanism. Design The OA model was established in a C57BL/6 mouse by the anterior cruciate ligament transaction method. OA was assessed using arthritis scoring and weightbearing parameters. Chondrocyte proliferation was detected by a CCK-8 assay. The levels of interleukin-6 (IL-6), IL-8 and tumor necrosis factor-α (TNF-α) in synovial fluid of the mice were measured by enzyme-linked immunosorbent assay. Results In OA mice, the arthritis score and weightbearing abilities were dramatically improved by LIPUS treatment. LIPUS also remarkably declined the levels of inflammatory cytokines IL-6, IL-8, and TNF-α in synovial fluid of OA mice. Moreover, LIPUS promoted chondrocyte proliferation and differentiation by activating focal adhesion kinase (FAK) signaling. Inhibition of FAK significantly blocked LIPUS-mediated cell proliferation and differentiation in vitro, as well as inflammation condition in OA mice. Conclusion LIPUS alleviates OA through promoting chondrocytes proliferation and differentiation by activating FAK, which could act as an intervening target for OA treatment.

scholarly journals Effect of Low Intensity Pulsed Ultrasound (LIPUS) on Tooth Movement and Root Resorption: A Prospective Multi-Center Randomized Controlled Trial

2020 ◽  
Vol 9 (3) ◽  
pp. 804 ◽  
Author(s):  
Tarek El-Bialy ◽  
Khaled Farouk ◽  
Terry D. Carlyle ◽  
William Wiltshire ◽  
Robert Drummond ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Controlled Trial ◽  
Control Group ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Control Side ◽  
Before And After ◽  
Group 1

The aim of this study was to evaluate the possible effect of low intensity pulsed ultrasound (LIPUS) on tooth movement and root resorption in orthodontic patients. Twenty-one patients were included in a split-mouth study design (group 1). Ten additional patients were included with no LIPUS device being used and this group was used as the negative control group (group 2). Group 1 patients were given LIPUS devices that were randomly assigned to right or left side on upper or lower arches. LIPUS was applied to the assigned side that was obtained by randomization, using transducers that produce ultrasound with a pulse frequency of 1.5 MHz, a pulse repetition rate of 1 kHz, and average output intensity of 30 mW/cm2. Cone-beam computed tomography (CBCT) images were taken before and after treatment. The extraction space dimensions were measured every four weeks and root lengths of canines were measured before and after treatment. The data were analyzed using paired t-test. The study outcome showed that the mean rate of tooth movement in LIPUS side was 0.266 ± 0.092 mm/week and on the control side was 0.232 ± 0.085 mm/week and the difference was statistically significant. LIPUS increased the rate of tooth movement by an average of 29%. For orthodontic root resorption, the LIPUS side (0.0092 ± 0.022 mm/week) showed a statistically significant decrease as compared to control side (0.0223 ± 0.022 mm/week). The LIPUS application accelerated tooth movement and minimized orthodontically induced tooth root resorption at the same time.

scholarly journals Effects of Low-Intensity Pulsed Ultrasound on the Proliferation of Human Umbilical Cord Mesenchymal Stem Cells and Their Chondrogenic Differentiation via Bmp2 /Smad Signaling Pathway

2020 ◽  
Author(s):  
Xuhong JIN ◽  
Hejie Wang ◽  
Jiaju Che ◽  
Xiafu Chen ◽  
Dengao Huang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Chondrogenic Differentiation ◽  
Blue Staining ◽  
Control Group ◽  
Human Umbilical Cord ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Proliferation Activity ◽  
Smad Signaling Pathway

Abstract BackgroundAlthough Low-intensity pulsed ultrasound (LIPUS) regiment has been applied to the treatment of cartilage injury clinically, the effect of LIPUS stress loading on the proliferation and differentiation of hUCMSCs is still controversial. Yet the specific mechanical mechanism of LIPUS stimulates cartilage differentiation of hUCMSCs has not been clarified.ObjectiveTo investigate the effects of low intensity pulsed ultrasound (LIPUS) on proliferation and chondrogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) via BMP2 /smad signaling pathway in vitro.MethodThe hUCMSCs at P4 were randomly divided into control group(no LIPUS), L30 group (30mW/cm2), L50 group (50mW/cm2), L80 group (80mW/cm2), each LIPUS group respectively loaded 5, 10, 20 min/d, the cell proliferation activity was detected by CCK-8 assay after continuous irradiation for 5 days. For chondrogenic differentiation, hUCMSCs were divided 4 groups as follows: control group, Noggin group, LIPUS group, Noggin+LIPUS group, LIPUS irradiation was given 50w/cm2, 5min/d. Cartilage formation after 3 weeks was evaluated by Alician blue staining for GAG and COL II immunohistochemical staining. Furthermore, RT-PCR analysis of Sox-9, COL II Aggrecan, BMP2, Smad1, Smad5 and Smad9. Result： (1) The proliferation activity of L50 group(50w/cm2 , 5min/d) was noticeably higher compared with other groups, Alcian blue staining of GAG , COL II fluorescent staining in the LIPUS group were much stronger, quantitative RT-PCR assays showed that the expression of COL II, GAG, Sox-9, smad1, smad5 and smad9 in LIPUS group was significantly increased. Conclusion：The suitable LIPUS irradiation can promote cell proliferation, 50mW/cm2 intensity at 5min/d is most significant. And it can promote chondrogenesis of hUCMSCs via upregulating MP2 /smads signaling pathway.

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