scholarly journals Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Satoshi Gondaira ◽  
Koji Nishi ◽  
Jumpei Fujiki ◽  
Hidetomo Iwano ◽  
Reina Watanabe ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Innate Immune Response ◽  
Quantitative Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Functional Expression ◽  
Innate Immune ◽  
Cytokine Gene Expression ◽  
Mycoplasma Bovis ◽  
Bovine Neutrophils

AbstractMycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. bovis. However, even though neutrophils accumulate in M. bovis infection, the interaction of M. bovis and neutrophils has not been fully elucidated. We attempted to elucidate the innate immune response of neutrophils stimulated with M. bovis and evaluate the transcriptome and functional analysis of bovine neutrophils stimulated with M. bovis. Proinflammatory cytokines, such as inducible nitric oxide (iNOS), which was the most increased gene in transcriptome analysis, were increased in quantitative polymerase chain reaction analysis of bovine neutrophils stimulated with live or heat-killed M. bovis. Nitric oxide and intracellular reactive oxygen species production of neutrophils stimulated with M. bovis was significantly increased. Neutrophils stimulated with M. bovis showed an increased ratio of nonapoptotic cell death compared to unstimulated controls. We demonstrated that neutrophil extracellular traps (NETs) formation was not recognized in neutrophils stimulated with live M. bovis. However, heat-killed M. bovis induced NETs formation. We also showed the interaction with M. bovis and bovine neutrophils regarding proinflammatory cytokine gene expression and functional expression related to NETs formation. Live and killed M. bovis induced innate immune responses in neutrophils and had the potential to induce NETs formation, but live M. bovis escaped NETs.

scholarly journals Exposure to artificial light at night alters innate immune response in wild great tit nestlings

2021 ◽  
Author(s):  
Ziegler Ann-Kathrin ◽  
Watson Hannah ◽  
Hegemann Arne ◽  
Meitern Richard ◽  
Canoine Virginie ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Immune Function ◽  
Innate Immune Response ◽  
Great Tit ◽  
Innate Immune ◽  
Artificial Light ◽  
Immune Challenge ◽  
Light At Night ◽  
Dark Night

The large-scale impact of urbanization on wildlife is rather well documented, however the mechanisms underlying the effects of urban environments on animal physiology and behaviour are still poorly understood. Here, we focused on one major urban pollutant - artificial light at night (ALAN) - and its effects on the capacity to mount an innate immune response in wild great tit Parus major nestlings. Exposure to ALAN alters circadian rhythms of physiological processes, by disrupting the nocturnal production of the hormone melatonin. Nestlings were exposed to a light source emitting 3 lux for seven consecutive nights. Subsequently, nestlings were immune-challenged with a lipopolysaccharide injection, and we measured haptoglobin and nitric oxide levels pre- and post-injection. Both haptoglobin and nitric oxide are important markers for innate immune function. We found that ALAN exposure altered the innate immune response, with ALAN nestlings having lower haptoglobin and higher nitric oxide levels after the immune-challenge compared to dark-night nestlings. Unexpectedly, nitric oxide levels were overall, lower after the immune-challenge than before. These effects were likely mediated by melatonin, since ALAN-treated birds had on average 49% lower melatonin levels than the dark-night birds. ALAN exposure did not have any clear effects on nestling growth. This study provides a potential physiological mechanism underlying the documented differences in immune function between urban and rural birds observed in other studies. Moreover, it gives evidence that ALAN exposure affects nestling physiology, potentially causing long-term effects on physiology and behaviour, which ultimately can affect their fitness.

scholarly journals Myeloid-Related Proteins Rapidly Modulate Macrophage Nitric Oxide Production during Innate Immune Response

2008 ◽  
Vol 181 (5) ◽  
pp. 3595-3601 ◽  
Author(s):  
Philippe Pouliot ◽  
Isabelle Plante ◽  
Marie-Astrid Raquil ◽  
Philippe A. Tessier ◽  
Martin Olivier
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Related Proteins

scholarly journals Enhanced Pathogenesis Caused by Influenza D Virus and Mycoplasma bovis Coinfection in Calves: a Disease Severity Linked with Overexpression of IFN-γ as a Key Player of the Enhanced Innate Immune Response in Lungs

2021 ◽  
Vol 9 (3) ◽  
Author(s):  
Adrien Lion ◽  
Aurélie Secula ◽  
Camille Rançon ◽  
Olivier Boulesteix ◽  
Anne Pinard ◽  
...  
Keyword(s):  
Immune Response ◽  
Respiratory Disease ◽  
Disease Severity ◽  
Innate Immune Response ◽  
Bovine Respiratory Disease ◽  
Innate Immune ◽  
Mycoplasma Bovis ◽  
Complex Interactions ◽  
Young Cattle ◽  
Ifn Γ

Bovine respiratory disease (BRD) is among the most prevalent diseases in young cattle. BRD is due to complex interactions between viruses and/or bacteria, most of which have a moderate individual pathogenicity.

scholarly journals Innate Immune Response to Intramammary Mycoplasma bovis Infection

2007 ◽  
Vol 90 (7) ◽  
pp. 3336-3348 ◽  
Author(s):  
A.C.W. Kauf ◽  
R.F. Rosenbusch ◽  
M.J. Paape ◽  
D.D. Bannerman
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Mycoplasma Bovis

scholarly journals Expression of innate immune response genes in the heart valves obtained from patients with infective endocarditis

2020 ◽  
pp. 81-82
Author(s):  
А.В. Цепокина ◽  
М.Ю. Синицкий ◽  
М.А. Асанов ◽  
Я.В. Казачек ◽  
А.В. Евтушенко ◽  
...  
Keyword(s):  
Immune Response ◽  
Infective Endocarditis ◽  
Innate Immune Response ◽  
Heart Valves ◽  
Quantitative Polymerase Chain Reaction ◽  
Mrna Level ◽  
Innate Immune ◽  
Control Group ◽  
Tlr2 Expression ◽  
Immune Response Genes

Изучены образцы створок клапанов сердца, полученные в ходе кардиохирургического вмешательства от 26 пациентов с установленным диагнозом «инфекционный эндокардит» (ИЭ), и от 12 пациентов без данной патологии (контрольная группа). Оценка уровня экспрессии генов врожденного иммунного ответа TLR1, TLR2, TLR4 и TLR6 проводилась методом количественной полимеразной цепной реакции. Установлено, что экспрессия гена TLR2 у пациентов с ИЭ практически не отличалась от контрольной группы, в то время как уровень мРНК генов TLR1, TLR4 и TLR6 в группе пациентов был значительно ниже, чем в контроле. We studied samples of heart valves obtained during cardiac surgery from 26 patients with infective endocarditis (IE) and 12 patients without this pathology (control group). The expression of innate immune response genes (TLR1, TLR2, TLR4 and TLR6) was evaluated by quantitative polymerase chain reaction. It was found that the TLR2 expression in patients with IE was similar to the control group, while the mRNA level of the TLR1, TLR4 and TLR6 genes in the patients was significantly lower compared to the control.

scholarly journals Spermine Causes Loss of Innate Immune Response toHelicobacter pyloriby Inhibition of Inducible Nitric-oxide Synthase Translation

2004 ◽  
Vol 280 (4) ◽  
pp. 2409-2412 ◽  
Author(s):  
Françoise I. Bussière ◽  
Rupesh Chaturvedi ◽  
Yulan Cheng ◽  
Alain P. Gobert ◽  
Mohammad Asim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Nitric Oxide Synthase ◽  
Innate Immune Response ◽  
Inducible Nitric Oxide Synthase ◽  
Innate Immune ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Type 1 Interferon (IFNα/β) and Type 2 Nitric Oxide Synthase Regulate the Innate Immune Response to a Protozoan Parasite

Immunity ◽  
1998 ◽  
Vol 8 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Andreas Diefenbach ◽  
Heike Schindler ◽  
Norbert Donhauser ◽  
Elke Lorenz ◽  
Tamás Laskay ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Immune Response ◽  
Nitric Oxide Synthase ◽  
Innate Immune Response ◽  
Protozoan Parasite ◽  
Innate Immune ◽  
Type 1 Interferon ◽  
Oxide Synthase
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