scholarly journals Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yi Zhang ◽  
Cheng Tian ◽  
Jiling Xiao ◽  
Lin Wei ◽  
Yun Tian ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Relative Abundance ◽  
Average Length ◽  
Plant Growth Promoting Rhizobacteria ◽  
Continuous Cropping ◽  
Trichoderma Asperellum ◽  
Control Effect ◽  
Total N ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. niveum (FON) is a soil-borne disease that seriously limits watermelon production. In the present study, Trichoderma asperellum (T. asperellum) M45a was shown to be an effective biocontrol agent against FW. In a pot experiment, the application of 105 cfu/g of T. asperellum M45a granules had an improved control effect on FW during the blooming period (up to 67.44%) in soils subjected to five years of continuous cropping with watermelon, while the average length of watermelon vines was also significantly improved (P < 0.05). Additionally, the acid phosphatase (ACP), cellulase (CL), catalase (CAT), and sucrase (SC) activities in the M45a-inoculation group were significantly higher than those in the control (CK) group, and transformation of the soil nutrients (total N, NO3-N, and available P) was significantly increased. Moreover, T. asperellum M45a inoculation reduced fungal diversity, increased bacterial diversity and especially enhanced the relative abundance of plant growth-promoting rhizobacteria (PGPR), such as Trichoderma, Sphingomonas, Pseudomonas, Actinomadura, and Rhodanobacter. Through functional prediction, the relative abundance of ectomycorrhiza, endophytes, animal pathotrophs, and saprotrophs in the fungal community was determined to be significantly lower than that observed in the M45a-treated soil. Correlation analysis revealed that Sphingomonas, Pseudomonas, and Trichoderma had the most differences in terms of microorganism abundance, and these differences were positively correlated with ACP, CL, CAT, and SC. These findings provide guidance for the use of fungicides to achieve microecological control of FW in continuously cropped watermelon plots.

scholarly journals Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt

2020 ◽  
Author(s):  
Yi Zhang ◽  
Cheng Tian ◽  
Jiling Xiao ◽  
Lin Wei ◽  
Yun Tian ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Relative Abundance ◽  
Average Length ◽  
Plant Growth Promoting Rhizobacteria ◽  
Continuous Cropping ◽  
Trichoderma Asperellum ◽  
Control Effect ◽  
Total N ◽  
P Transformation ◽  
Plant Growth Promoting

Abstract Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. niveum (FON) is a soil-borne disease that seriously limits watermelon production. In the present study, the Trichoderma asperellum (T. asperellum) M45a was shown to be an effective biocontrol agent against Fusarium wilt (FW). In a pot experiment, the application of 105 cfu/g of T. asperellum M45a granules had better control effect on FW at the blooming period (up to 67.44%) from soils subjected to five years of continuous cropping with watermelon, while the average length of watermelon vine was also significantly improved(P < 0.05). Additionally, the acid phosphatase (ACP), cellulase (CL), catalase (CAT) and sucrase (SC) activities in the M45a-inoculation group were significantly higher than in the control (CK) group, and the soil nutrients (total N, NO3-N, and available P) transformation was significantly increased. Moreover, T. asperellum M45a inoculation reduced fungal diversity and increased bacterial diversity, especially enhancing the relative abundance of PGPR (plant growth promoting rhizobacteria), such as Trichoderma, Sphingomonas, Pseudomonas, Actinomadura and Rhodanobacter. Through functional prediction, the relative abundance of Ectomycorrhizal, Endophyte, Animal pathotroph and saprotroph in fungal community was determined to be significantly lower than observed in the M45a-treated soil. Correlation analysis revealed that Sphingomonas, Pseudomonas and Trichoderma had the most differences in microorganisms abundance, positively correlated with ACP, CL, CAT and SC. These findings will provide ecological fungicide advice for microecological control of FW in continuous cropping watermelon.

scholarly journals Soil inoculation of Trichoderma asperellum M45a regulates rhizosphere microbes and triggers watermelon resistance to Fusarium wilt

2020 ◽  
Author(s):  
yi zhang ◽  
Cheng Tian ◽  
Jiling Xiao ◽  
Lin Wei ◽  
Yun Tian ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Relative Abundance ◽  
Average Length ◽  
Plant Growth Promoting Rhizobacteria ◽  
Continuous Cropping ◽  
Trichoderma Asperellum ◽  
Control Effect ◽  
Total N ◽  
P Transformation ◽  
Plant Growth Promoting

Abstract Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. niveum (FON) is a soil-borne disease that seriously limits watermelon production. In the present study, the Trichoderma asperellum ( T. asperellum ) M45a was shown to be an effective biocontrol agent against Fusarium wilt (FW). In a pot experiment, the application of 10 5 cfu/g of T. asperellum M45a granules had better control effect on FW at the blooming period (up to 67.44%) from soils subjected to five years of continuous cropping with watermelon, while the average length of watermelon vine was also significantly improved(P<0.05). Additionally, the acid phosphatase (ACP), cellulase (CL), catalase (CAT) and sucrase (SC) activities in the M45a-inoculation group were significantly higher than in the control (CK) group, and the soil nutrients (total N, NO3-N, and available P) transformation was significantly increased. Moreover, T. asperellum M45a inoculation reduced fungal diversity and increased bacterial diversity, especially enhancing the relative abundance of PGPR (plant growth promoting rhizobacteria), such as Trichoderma, Sphingomonas , Pseudomonas , Actinomadura and Rhodanobacter . Through functional prediction, the relative abundance of Ectomycorrhizal, Endophyte, Animal pathotroph and saprotroph in fungal community was determined to be significantly lower than observed in the M45a-treated soil. Correlation analysis revealed that Sphingomonas , Pseudomonas and Trichoderma had the most differences in microorganisms abundance, positively correlated with ACP, CL, CAT and SC. These findings will provide ecological fungicide advice for microecological control of FW in continuous cropping watermelon.

scholarly journals Control of Fusarium Disease in Onion with Plant Growth Promoting Rhizobacteria (PGPR) and Mycorrhizae and Its Effect on Growth and Yield of Onion

2019 ◽  
Vol 7 (1) ◽  
pp. 23
Author(s):  
Salamiah Salamiah ◽  
Muhammad Anton Ciptady ◽  
Chatimatun Nisa
Keyword(s):  
Fusarium Wilt ◽  
Combined Treatment ◽  
Plant Growth Promoting Rhizobacteria ◽  
Wilt Disease ◽  
Growth And Yield ◽  
Randomized Design ◽  
Plant Growth Promoting ◽  
Mycorrhizal Inoculation ◽  
Growth Promoting ◽  
Inhibition Ability

<p>The productivity of onion in Indonesia is generally low due to fusarium wilt disease.  Biological controls can be applied using PGPR and Mycorrhizae. The purpose of this research was understand the interaction between PGPR and Mycorrhizal inoculation against fusarium wilt intensity as well as the growth and yield of onions. The isolation of <em>Fusarium oxysporum</em> f.sp <em>cepae</em> and PGPR, followed by the tests of PGPR inhibition ability, phosphate solvent and HCN compound productivity. The method used in the field was a completely randomized design (CRD) with 2 replications. Results showed that the combination of PGPR and mycorrhizae as a whole was unable to suppress <em>Fusarium</em> wilt disease, but had significant effect to postpone the incubation period (26,19 days after inoculation) and increase the growth and yield of onion compared to the onion plants infected with <em>Fusarium</em> but without the combined treatment of PGPR and mycorrhizae and the PGPR treatment and mycorrhizal treatment as single treatments; the application of mycorrhizae as the single factor had a very significant effect on the number of bulbs, but had no significant effect on the inhibition of fusarium wilt intensity as well as the growth and yield of onions.</p>

scholarly journals Plant Growth Promoting Rhizobacteria Alleviate Aluminum Toxicity and Ginger Bacterial Wilt in Acidic Continuous Cropping Soil

2020 ◽  
Vol 11 ◽  
Author(s):  
Shuting Zhang ◽  
Qipeng Jiang ◽  
Xiaojiao Liu ◽  
Liehua Liu ◽  
Wei Ding
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Bacterial Wilt ◽  
Aluminum Toxicity ◽  
Bacterial Community Composition ◽  
Plant Growth Promoting Rhizobacteria ◽  
Continuous Cropping ◽  
Aluminum Ions ◽  
Plant Growth Promoting ◽  
Growth Promoting

Long-term monoculture cropping is usually accompanied by soil acidification and microbial community shifts. Soil aluminum ions are dissolved under acidic condition (pH &lt; 5.0), and the resulting aluminum bioavailability can cause toxic effects in plants. In this study, we investigated the bacterial community compositions and aluminum toxicity in fields monocultured with ginger for 35 years, 15 years, and 1 year. Within these fields are ginger plants without and with ginger bacterial wilt disease. The results confirmed that the degree of aluminum toxicity in the diseased soil was more severe than that in the healthy soil. Continuous cropping can significantly increase the bacterial diversity and change the bacterial community composition of ginger rhizosphere soil. The relative abundance of plant growth-promoting rhizobacteria (PGPRs) was increased in the soils used for the continuous cropping of ginger. Additionally, aluminum toxicity had a significant positive correlation with Bacillus, Pseudomonas, Arthrobacter, and Serratia in healthy soils. Based on these results, aluminum stress may stimulate the increase of PGPRs (Bacillus, Pseudomonas, Arthrobacter, and Serratia), thereby alleviating ginger aluminum toxicity and bacterial wilt in extremely acidic soil (pH &lt; 4.5).

scholarly journals Rhizobacteria Associated with a Native Solanaceae Promote Plant Growth and Decrease the Effects of Fusariumoxysporum in Tomato

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 579
Author(s):  
Carmen Sanjuana Delgado-Ramírez ◽  
Rufina Hernández-Martínez ◽  
Edgardo Sepúlveda
Keyword(s):  
Plant Growth ◽  
Fusarium Wilt ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Positive Effects ◽  
Alternative Approach ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Plant growth-promoting rhizobacteria are often utilized to improve crop health and productivity. Nevertheless, their positive effects can be hindered if they fail to withstand the environmental and ecological conditions of the regions where they are applied. An alternative approach to circumvent this problem is a tailored selection of bacteria for specific agricultural systems. In this work, we evaluated the plant growth promoting and pathogen inhibition activity of rhizobacteria obtained from the rhizosphere of Mariola (Solanum hindsianum), an endemic shrub from Baja California. Eight strains were capable of inhibiting Fusarium oxysporum in vitro, and thirteen strains were found to possess three or more plant-growth-promotion traits. Molecular identification of these strains, using 16 s rRNA partial sequences, identified them as belonging to the genera Arthrobacter, Bacillus, Paenibacillus, Pseudomonas, and Streptomyces. Finally, the effect of selected plant growth-promoting rhizobacteria (PGPR) strains on the growth and suppression of Fusarium wilt in tomato was evaluated. Results showed that these strains improved tomato plants growth under greenhouse conditions and reduced Fusarium wilt effects, as reflected in several variables such as length and weight of roots and stem. This work highlights the potential of native plants related to regionally important crops as a valuable source of beneficial bacteria.

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