scholarly journals Correlation of sperm DNA damage with blastocyst formation: systematic review and meta-analysis

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Prashanth K. Adiga ◽  
Srisailesh Vitthala ◽  
Shivaranjeni
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Meta Analysis ◽  
Main Body ◽  
Sperm Dna Fragmentation ◽  
Blastocyst Formation ◽  
Male Factor ◽  
Sperm Dna Damage ◽  
Sperm Dna

Abstract Background The routine semen analysis fails to detect sperm DNA damage which contributes to the majority of male factor infertility. Sperm DNA fragmentation test (DFI) measures the sperm DNA damage. Blastocyst formation is an important step in IVF ± ICSI. At present, the literature lacks any data that correlates DFI and blastocyst formation. Main body of the abstract We searched MEDLINE and other databases till 2020 for the studies that reported on sperm DNA damage and blastocyst formation in assisted reproductive technology (ART). The outcomes analyzed were (1) a comparison of blastulation rates in high DFI and low DFI groups. (2) Comparison of blastulation rates in high DFI and low DFI groups based on (a) different sperm DNA fragmentation assays (COMET, SCD, SCSA, TUNEL), (b) different types of ART (IVF/IVF + ICSI/ICSI). 10 studies were included in this review. A non-significant increase in the blastocyst formation was observed in high DFI group (OR = 0.70; 95% CI = 0.4 to 1.21; P = 0.20) and with SCD and TUNEL assays. Short conclusion Our study emphasizes on sperm DNA fragmentation (sperm DNA damage) as an important marker of blastocyst formation. The results of this meta-analysis suggest that the high sperm DNA fragmentation may not adversely affect the blastocyst formation.

Evaluation of Sperm DNA Fragmentation via Halosperm Technique and TUNEL Assay Before and After Cryopreservation

2019 ◽  
Vol 26 (12) ◽  
pp. 1575-1581 ◽  
Author(s):  
Senay Cankut ◽  
Turgay Dinc ◽  
Mehmet Cincik ◽  
Guler Ozturk ◽  
Belgin Selam
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Tunel Assay ◽  
University Hospital ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sperm Dna Fragmentation ◽  
Liquid Form ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Before And After

Aim: Human sperm DNA fragmentation is one of the factors suggested for male infertility. The ratio of sperm DNA damage in semen may adversely affect both the fertilization rate and the embryo development of in vitro fertilization/ intracytoplasmic sperm injection cycles. Sperm cryopreservation both increases the success rates in assisted reproductive techniques (ARTs) and contributes to the preservation of fertility before testis surgery, chemotherapy, and radiotherapy. The aim of the current study is to determine sperm DNA fragmentation, following cryopreservation. Methods: A cross-sectional, observational study was conducted at a university hospital infertility clinic. One hundred (n = 100) volunteer fertile men (ages between 21 and 39 years) with normozoospermic sperm parameters were involved in the current study. Sperm DNA damage was evaluated with the Halosperm technique and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Fresh samples were studied in liquid form. The remaining samples were kept frozen and then thawed after 1 month and reevaluated with the Halosperm technique and TUNEL assay. Results were then compared between the fresh and frozen samples. Results: Sperm DNA fragmentation results with the Halosperm technique both before and after cryopreservation were 25% (5%-65%) and 40% (6%-89%), respectively, with a statistically significant increase (15%; P < .001). Sperm DNA fragmentation results by TUNEL assay before and after cryopreservation were 17% (3%-43%) and 36% (7%-94%), respectively, with a statistically significant increase (19%; P <.001). Conclusion: The current data demonstrate increased sperm DNA damage after cryopreservation. Further studies may contribute to development of less harmful techniques and cryoprotectants in order to improve the results of ART.

scholarly journals Sperm DNA Fragmentation: Origin and Impact on Human Reproduction

2011 ◽  
Vol 2 (2) ◽  
pp. 88-108 ◽  
Author(s):  
Ralf R. Henkel ◽  
Daniel R. Franken
Keyword(s):  
Dna Damage ◽  
Environmental Factors ◽  
Dna Fragmentation ◽  
Human Reproduction ◽  
Sperm Dna Fragmentation ◽  
Permanent Damage ◽  
Sperm Dna Damage ◽  
Male Genome ◽  
Sperm Dna ◽  
Early Abortion

Sperm DNA can be damaged due to a multitude of different noxae, which include disease, and occupational and environmental factors. Depending on the magnitude of the damage, such lesions may be repaired by the oocyte or the embryo. If this is not possible, a permanent damage can be manifested leading to mutations of the male genome. In cases where the oocyte or the embryo does not counter these damages to the male genome in terms of repair or an early abortion, sperm DNA damage and fragmentation can be a cause of numerous diseases including childhood cancer.

scholarly journals Effect of sperm DNA fragmentation on ICSI outcome: A prospective study

2020 ◽  
Vol 3 (2) ◽  
pp. 127-131
Author(s):  
Lakshamanan Saravanan ◽  
Mahalakshmi Saravanan ◽  
Ramya Harish ◽  
Nidhi Sharma
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
No Reduction ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Secondary Objective ◽  
A Prospective Study ◽  
Thesis Work ◽  
Embryo Grade

Aim and objectives: The primary aim was to measure the sperm DNA damage and to study the magnitude of sperm DNA damage. Secondary objective was to study the effect of sperm DNA fragmentation on Day 5 Blastocyst expansion (graded 1-5). Results: There is an increase in sperm DNA fragmentation with an increase in age. Increased sperm DNA fragmentation is also associated with abnormal motility and morphology in semen samples. However, there is no reduction in expansion or grade of blastocyst. Conclusion: Sperm DNA fragmentation testing is a useful investigation in unexplained infertility. However, Sperm DNA fragmentation has no significant association with Day 5 embryo grade in ICSI cycles. Thesis work of Fellowship in Reproductive Medicine student: Dr. Ramya Harish

scholarly journals Mitigating the Effects of Oxidative Sperm DNA Damage

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 589
Author(s):  
Taylor Pini ◽  
Rachel Makloski ◽  
Karen Maruniak ◽  
William B. Schoolcraft ◽  
Mandy G. Katz-Jaffe
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Post Treatment ◽  
Semen Parameters ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sperm Dna Fragmentation ◽  
Success Rates ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Cost Effective Treatment

Sperm DNA damage is correlated with reduced embryo development and increased miscarriage risk, reducing successful conception. Given its links with oxidative stress, antioxidants have been investigated as a potential treatment, yet results are conflicting. Importantly, individual antioxidants are not identical in composition, and some compounds may be more effective than others. We investigated the use of the polyphenol-rich, high-antioxidant-capacity fruit acai as a treatment for elevated sperm DNA fragmentation (>16%), measured by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Following ≥ 74 days of treatment, we observed a significant decrease in sperm DNA fragmentation (−17.0% ± 2.5%) to 11.9 ± 1.7% (0–37%), with a 68.6% success rate (defined as post-treatment TUNEL < 16%). Post-treatment decreases in DNA fragmentation and success rates were not significantly impacted by low motility and/or concentration, or exceptionally high (> 25%) TUNEL. Treatment significantly reduced concentration in men with normal semen parameters, but 88% remained normal. Overall, successful treatment was not associated with age, semen parameters or TUNEL result at baseline. However, body mass index was significantly higher in nonresponders at baseline. This study provides evidence of a low-cost, effective treatment for elevated sperm DNA damage using acai.

scholarly journals Can Utilization of Sperm DNA Fragmentation (SDA) Tests in Combination with Time Lapse Microscopy Help in Improving ICSI Implantation Rates in Unexplained Recurrent Implantation Failures Utilizing Double Stranded SDA as the Causative Factor for the same

2020 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Time Lapse ◽  
Sperm Dna Fragmentation ◽  
Time Lapse Microscopy ◽  
Sperm Dna ◽  
Implantation Rates ◽  
Embryo Kinetics

Over 50% of intracytoplasmic sperm injection (ICSI) cycles don’t display implantation. Hence laboratories make their maximum efforts to select the best embryos as far as implantation enhancement is concerned. Further utilization of available technologies like time lapse recording have been made in a large number of artificial reproductive technology (ART) centres. Various studies that utilize embryo kinetics have implicated that time when embryo cleavage may prove to be an important factor that determines the implantation potential of an embryo. With this variety of algorithms mathematic wise have been used to forecast which the best embryos are for transfer. But the efficacy of these might be influenced by multiple confounding factors. Thus work on biomarkers that can forecast good ART warrants newer embryo selection basis. Regarding conventional ICSI, typical standard routine semen analysis involving sperm concentration, motility and morphology does not predict the implantation percentages in an ICSI cycle. Once sperm DNA fragmentation (SDA) methods were inducted they appeared to hold promise in forecasting good ART success. Although certain studies utilizing various techniques like TUNEL. SCSA, SCD proved a relation existed between DNA damage and implantation rates in ICSI but the same was contradicted by others. With this it was thought that bias between evaluation of ejaculate and motile sperm picked up for ICSI, as is known regarding absence of positive association of sperm motility and DNA fragmentation. Thus study by Casanovas et.al., tried to find if there is any correlation of single stranded (ssSDA) and double stranded (dsSDA) sperm DNA damage that might forecast ICSI success and utilizing Neutral Comet Assays along with help of time lapse technology they found that double stranded sperm influenced delay in embryo formation as seen by embryo kinetics and thus interfere with implantation rates. Reproduction of these findings might help in getting a standard for getting best embryos selected in ICSI utilizing SDA and time lapse microscopy.

scholarly journals Antioxidant treatment of increased sperm DNA fragmentation: Complex combinations are not more successful

2020 ◽  
Vol 92 (4) ◽  
Author(s):  
Cevahir Ozer
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Single Agent ◽  
Included Study ◽  
Sperm Dna Fragmentation ◽  
Antioxidant Treatment ◽  
Sperm Dna Damage ◽  
Treatment Groups ◽  
Sperm Dna ◽  
Significant Difference

Objective: Oral antioxidant supplementation is part of the treatment of infertility associated with oxidative stress-related sperm damage. It is possible to assume that the combined use of antioxidants will be better than single agent use. The purpose of this study was to compare the effectiveness of different antioxidant combinations in infertile men with increased sperm DNA fragmentation. Materials and methods: We retrospectively reviewed the records of 637 patients who underwent antioxidant support therapy for increased sperm DNA damage between 2014 and 2019. Patients with DNA damage of 30% or more were included study. Result: A total of 163 patients with follow-up data and who fulfilled the study criteria were included in the study. There were four different treatment groups. No statistically significant differences were found between the groups. After 3 months of antioxidant treatment, there was a statistically significant decrease in sperm DNA damage in all treatment groups. However, there was no statistically significant difference between the treatment groups. Conclusions: The complexity of the antioxidant combination may not contribute to the success of the treatment or may cause possible side effects, increase the cost of treatment and decrease patient compliance.

scholarly journals The study of spermatic DNA fragmentation and sperm motility in infertile subjects

2013 ◽  
Vol 85 (1) ◽  
pp. 8 ◽  
Author(s):  
Giuseppina Peluso ◽  
Alessandro Palmieri ◽  
Pietro Paolo Cozza ◽  
Giancarlo Morrone ◽  
Paolo Verze ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Nuclear Dna ◽  
Inverse Correlation ◽  
World Health ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Spermatozoa Motility ◽  
Sperm Dna

Introduction: Although the pathophysiology of the testicular damage associated with varicocele remains unclear, sperm DNA damage has been identified as a potential explanation for this cause of male infertility. The current study was designed to determine the extent of sperm nuclear DNA damage in patients with varicocele, and to examine its relationship with parameters of seminal motility. Materials and method: Semen samples from 60 patients with clinical varicocele and 90 infertile men without varicocele were examined. Varicocele sperm samples were classified as normal or pathological according to the 1999 World Health Organizzation guidelines. Sperm DNA damage was evalutated using the Halosperm kit, an improved Sperm Chromatin Dispersion (SCD) test. Results: The DNA fragmentation index (DFI: percentage of sperm with denatured nuclei) values was significantly higher in patients with varicocele, either with normal or abnormal (DFI 25.8 &plusmn; 3.2 vs 17.4 &plusmn; 2.8 - P &lt; 0,01) semen profiles. In addition, an inverse correlation was found between spermatic motility and the degree of spermatic DNA fragmentation in patients with clinical varicocele. Conclusions: Varicocele is associated with high levels of DNA-damage in spermatozoa. In addition, in subjects with varicocele, abnormal spermatozoa motility is associated with higher levels of sperm DNA fragmentation. DNA fragmentation may therefore be an essential additional diagnostic test that should be recommended for patients with clinical varicocele.

scholarly journals A cost for high levels of sperm competition in rodents: increased sperm DNA fragmentation

2016 ◽  
Vol 283 (1826) ◽  
pp. 20152708 ◽  
Author(s):  
Javier delBarco-Trillo ◽  
Olga García-Álvarez ◽  
Ana Josefa Soler ◽  
Maximiliano Tourmente ◽  
José Julián Garde ◽  
...  
Keyword(s):  
Dna Damage ◽  
Sperm Competition ◽  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Physical And Chemical

Sperm competition, a prevalent evolutionary process in which the spermatozoa of two or more males compete for the fertilization of the same ovum, leads to morphological and physiological adaptations, including increases in energetic metabolism that may serve to propel sperm faster but that may have negative effects on DNA integrity. Sperm DNA damage is associated with reduced rates of fertilization, embryo and fetal loss, offspring mortality, and mutations leading to genetic disease. We tested whether high levels of sperm competition affect sperm DNA integrity. We evaluated sperm DNA integrity in 18 species of rodents that differ in their levels of sperm competition using the sperm chromatin structure assay. DNA integrity was assessed upon sperm collection, in response to incubation under capacitating or non-capacitating conditions, and after exposure to physical and chemical stressors. Sperm DNA was very resistant to physical and chemical stressors, whereas incubation in non-capacitating and capacitating conditions resulted in only a small increase in sperm DNA damage. Importantly, levels of sperm competition were positively associated with sperm DNA fragmentation across rodent species. This is the first evidence showing that high levels of sperm competition lead to an important cost in the form of increased sperm DNA damage.

scholarly journals Docosahexaenoic acid in the treatment of male infertility caused by high sperm DNA fragmentation

2021 ◽  
Vol 21 (4) ◽  
pp. 89-97
Author(s):  
I. V. Vinogradov ◽  
A. R. Zhivulko
Keyword(s):  
Dna Damage ◽  
Docosahexaenoic Acid ◽  
Male Infertility ◽  
Dna Fragmentation ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna Damage ◽  
Male Accessory Glands ◽  
Accessory Glands ◽  
Sperm Dna ◽  
High Level

Introduction. Antioxidant supplementation therapy continues to be the main treatment for male infertility associated with high level of sperm DNA damage. Docosahexaenoic acid (DHA) is one of the most promising components of antioxidant supplementation therapy. It also has anti-inflammatory properties that makes it interesting for treatment of patients with high level of sperm DNA damage and inflammation in male accessory glands.Materials and methods.One hundred and seventeen (117) infertile patients with high level of sperm DNA damage were recruited for this randomized, double blind, placebo-controlled study. Semen analysis, MAR-test, SCD test and sperm cryotolerance test were performed to all patients. Subjects were divided into 2 groups with high (>1 mln / ml) and low (<1 mln / ml) semen leucocyte concertation and then randomized into 2 subgroups of active treatment and 2 placebo subgroups. The active treatment subgroups received 1470 mg / day of DHA for 3 months. The placebo group received placebo for the same period. Laboratory tests were repeated after the treatment course had been finished.Results. Statistically significant increase in motility (42 % (25–61 %) vs 25 % (15–47 %), p <0.05), vitality (73 % (63–81 %) vs 41 % (35–64 %), p <0.05), decrease in sperm DNA fragmentation level (21 % (12–28 %) vs 33 % (25–39 %), p <0.05) and leucocyte concentration (1 million / ml (0.7–1.7 million / ml) vs 1,5 million / ml (1.1–2.1 million / ml), p <0.05) were observed in the subgroup with male accessory glands inflammation after treatment. Motility (15 % (8–19 %) vs 8 % (5–11 %), p <0.05) and vitality (37 % (25–46 %) vs 24 % (17–40 %), p <0.05) in this subgroup after a sperm cryotolerance test increased as well. In the subgroup with low semen leucocyte concertation statistically significant increase in motility (43 % (27–63 %) vs 34 % (21–54 %), p <0.05), vitality (77 % (66–85 %) vs 65 % (54.5–76.0 %), p <0.05) and decrease of sperm DNA fragmentation level (9 % (5.5–20.0 %) vs 25 % (18–33 %), p <0.05) were observed. DHA supplementation also resulted in statistically significant increase in motility (17 % (10–23 %) vs 6 % (5.0–10.5 %), p <0.05) and vitality (41 % (32.5–53.0 %) vs 37 % (30–49 %), p <0.05) after a sperm cryotolerance test in that subgroup.Conclusion. DHA supplementation therapy increases motility, vitality, sperm cryotolerance and decreases sperm DNA fragmentation regardless of the presence of an inflammatory process in male accessory glands.

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