Amelioration of complete Freund’s adjuvant-induced arthritis by Calotropis procera latex in rats

Abstract Background Rheumatoid arthritis is the most common cause of disability, affecting 0.3–1% of the adult population worldwide. The latex of Calotropis procera possesses potent anti-inflammatory as well as analgesic properties. In light above facts, the present study was designed to evaluate anti-arthritic activity of Calotropis procera latex in complete Freund's adjuvant (CFA)-induced arthritis in Wistar albino rats. Complete Freund's adjuvant was injected into the left hind paw on day 0, and treatment of prednisolone and Calotropis procera latex was given from day 0 to 28. Various biochemical, hematological and functional parameters as well as radiological and histopathological changes of joint along with body weight and paw volume were measured. Results Calotropis procera treatment significantly lowered paw volume in CFA-induced arthritic rats. Significant improvement was observed in functional, biochemical and hematological parameters in Calotropis procera-treated rats. However, the body weight remained unaffected. Histological and radiographical examination of synovial joints in Calotropis procera-treated animals exhibited less synovial hyperplasia, infiltration and accumulation of inflammatory cell in synovial fluid, cartilage and bone erosion and joint space narrowing. Conclusion Calotropis procera latex possesses anti-arthritic activity, which is facilitated by modulation in the level of inflammatory mediators and oxidative stress. The improvement in hematological as well as biochemical parameters might be reflected on functional, histopathological, radiological changes and thereby disease progression.

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ERDOSTEINE: AN EFFECTIVE ANTIOXIDANT FOR PROTECTING COMPLETE FREUND’S ADJUVANT INDUCED ARTHRITIS IN RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i10.42365 ◽

2021 ◽

pp. 71-75

Author(s):

BANYLLA SYNMON ◽

SANHATIDUTTA ROY ◽

SUTAPA BISWAS MAJEE ◽

MEGHNA PAUL ◽

SANDIPAN DASGUPTA

Keyword(s):

Body Weight ◽

Test Group ◽

The Body ◽

Complete Freund’S Adjuvant ◽

Control Group ◽

Albino Rats ◽

Standard Group ◽

Freund’S Adjuvant ◽

Complete Freund's Adjuvant ◽

Freund's Adjuvant

Objective: The objective of this study was to evaluate the protective effect of Erdosteine on complete freund’s adjuvant (CFA) induced arthritic rats. Methods: Wistar Albino rats of 100–250 g were divided into five groups (n=6) and administered with 0.1 ml of CFA subcutaneously into the left hind paw except the negative control group. The standard group received methotrexate (MTX) 0.075 mg/kg body weight orally. Besides, the test groups received Erdosteine orally at a dose 10 mg/kg and 20 mg/kg bodyweight for 12 days. The changes in body weight, paw volume, hematological parameters, radiographical, and histological findings were the indicators to evaluate the efficacy of the test product. Discussion: Significant change in the body weight, paw volume, radiographical, hematological, and histological parameters were observed which supports the remarkable reduction of the arthritic development in the standard and test groups compared to the untreated group. However, the test group (Erdosteine) with the dose 20 mg/kg shows to be more potent than the test group (Erdosteine) with a dose 10 mg/kg and the standard group (MTX) to reduce the arthritic effect. Results: The test group with 20 mg/kg Erdosteine showed much better outcome than the standard group at significant (p<0.05). Therefore, Erdosteine acting as an anti-inflammatory and anti-oxidant is effective at a dose 20 mg/kg in treating the progression of rheumatoid arthritis in rats.

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Evaluation of Anti-arthritic Potential of Pisonia grandis R. Br Against Complete Freund’s Adjuvant (CFA) Induced Albino Wistar Rats

Phytothérapie ◽

10.3166/phyto-2018-0078 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S96-S103

Author(s):

R. Karthik ◽

D. Arul Ananth ◽

N. Gayathri ◽

L. Dinesh Kumar ◽

T. Sivasudha

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Complete Freund’S Adjuvant ◽

Weight Changes ◽

Markers Of Inflammation ◽

Freund’S Adjuvant ◽

Complete Freund's Adjuvant ◽

Freund's Adjuvant ◽

Pisonia Grandis

Medicinal plants have been employed in the treatment of human ailments since time immemorial. Arthritis is a joint disorder affecting the subchondral bone and cartilage. The continuous action of reactive oxygen species and inflammatory mediators are also reported to cause further damage to the joints by immunological activation. DriedPisonia grandisleaves were powdered for the extraction process. Albino Wistar rats were randomly divided into five groups with six animals in each group. Groups IV and V were complete Freund’s adjuvant (CFA) induced arthritic rats which receivedPisonia grandisethanol extract (PGEE; 250 and 500 mg/kg b.w. [body weight]) respectively from 15th to 35th day of induction of CFA induced arthritis. PGEE protects rats against primary and secondary arthritic lesions, body weight changes, and hematological perturbations induced by CFA. The serum markers of inflammation and arthritis, such as C-reactive protein and rheumatoid factor, were also reduced in the PGEE-treated arthritic rats. High performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) analysis of PGEE detected the presence of polyphenolic compounds such as resveratrol, quercetin, and naringenin. The study confirmed that presence of bioactive phytocompounds in ethanolic extract ofPisonia grandisR. Br. Overall, PGEE was observed to be a potent agent reducing arthritis-mediated cartilage/bone degradation, inflammation, and associated stress in vivo, which gives cause for further research.

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