MR quantification of abnormal stromal enhancement in the periphery of invasive breast tumors

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 636-636
Author(s):  
N. Nabavizadeh ◽  
C. Klifa ◽  
D. Newitt ◽  
Y. Lu ◽  
J. Hattangadi ◽  
...  
Keyword(s):  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Disease Free Survival ◽  
Normal Breast ◽  
Intensity Change ◽  
High Signal ◽  
Breast Cancer Patients ◽  
Post Contrast ◽  
Tumor Region ◽  
The Mean

636 Background: In dynamic contrast-enhanced MRI (DCEMRI), properties of tissue microvasculature and overall vascularity can be quantified using kinetics of contrast enhancement. It has been shown that areas with high signal enhancement ratio (SER) were significantly correlated with high tumor vascularity, and that mean SER in nearby non-cancerous breast stroma was significantly associated with disease free survival. No universally accepted methods exist to determine the optimal extent of histologically normal breast tissue to treat. Although a relationship between normal tissue SER and recurrence has been observed, the spatial relationship of enhancement within the non-cancerous stroma with respect to proximity of the tumor has not been shown. We hypothesized that abnormalities in vasculature exist within the histologically normal breast stroma that can be detected by measuring changes in enhancement intensity. Methods: We performed a secondary analysis of pre-treatment DCEMRI data from 27 invasive breast cancer patients. An automated, user-independent program was used to segment breast tissue from all other structures. A SER threshold was set to identify invasive tumor regions, and a tumor proximity map was generated giving the 3D distance of each normal breast tissue voxel to the nearest tumor voxel. A percent enhancement (PE) map was calculated for the normal stroma using the signal intensity change between the pre-contrast and post-contrast images. The proximity and PE maps were then combined to measure breast tissue enhancement at various distances from the tumor. Results: PE levels in normal breast tissue situated within 2 cm of the tumor region were significantly higher than at all more distant regions. Pairwise comparisons with Turkey's adjusted p-values indicated that the mean PE at 0 cm-1 cm is significantly higher than all other distant levels (p = < 0.0001) and the mean PE at 1 cm-2 cm is significantly higher than 5 cm-6 cm (p = 0.006). Conclusions: Here, we show that the normal-appearing breast stroma within 0 cm to 2 cm of a primary tumor exhibits higher enhancement levels than stroma located far from the tumor. These results suggest that tissue surrounding the tumor region may contain tumor-related angiogenesis. These findings could help personalize surgery and radiation techniques. No significant financial relationships to disclose.

Temperature Variation in Breast Tissue Model With and Without Tumor Based on Porous Media

2021 ◽  
Vol 4 (1) ◽  
pp. 61-75
Author(s):  
Sharmila Shrestha ◽  
Gokul K.C. ◽  
Dil Bahadur Gurung
Keyword(s):  
Porous Media ◽  
Temperature Variation ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Anterior Part ◽  
Skin Surface ◽  
Normal Breast ◽  
The Finite Element Method ◽  
Porous Media Model ◽  
Tumor Region

The human body is made by 200 different types of cells, which are separated by voids. Blood supplies the nutrients and minerals to all cells within the tissue through these voids. The breast tissue is treated as a porous media in the study. Tumor includes the vascular (blood) and the extra-vascular (solid) regions. The porosity of a tumor is higher than normal tissue. The present work deals with the temperature variation of normal and tumorous breast tissue based on porous media. The finite element method is used to solve the two-dimensional bio-heat equation. The results show that the temperature profile of normal breast tissue in the porous media model is almost identical with the conventional bio-heat model at correction factor is equal to 0:6. The temperature of tumor region in the porous media model is slightly lower than the conventional bio-heat model. When the porosity is increased, the temperature of normal breast tissue is increased. But in tumorous breast tissue, the temperature is slightly increased in skin surface to anterior part of the tumor and slightly decreased in tumor region. The temperature of normal and tumorous breast tissue is increased when metabolism, blood velocity, and room temperature are increased in the porous media model. The central temperature of the tumor region reaches a steady state faster than anterior and posterior temperature of both normal and tumorous breast tissue in conventional bio-heat model and porous media model.

Carcinoembryonic Antigen, Ferritin, Tissue Polypeptide Antigen, and Ca15/3 in Breast Cancer: Relationship between Carcinoma and Normal Breast Tissue

1986 ◽  
Vol 1 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Massimo Gion ◽  
Riccardo Mione ◽  
Ruggero Dittadi ◽  
Luciano Griggio ◽  
Gabriele Munegato ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Normal Tissue ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Tissue Sample ◽  
Normal Breast ◽  
Cancer Tissue ◽  
Breast Cancer Patients ◽  
Breast Tissue Sample ◽  
Supply Information

The study of tumor markers in breast cancer tissue may supply information on the tumor's biological features and its clinical behaviour. Forty-nine primary breast cancer patients are evaluable to date. CEA, ferritin, TPA and CA15/3 were measured with radioimmu-nometric methods in the cytosol of carcinoma and normal tissue from the same breast. The concentrations of the four markers were higher in the tumor than in normal tissue in 42/49 cases for CEA, 47/49 for ferritin, 42/49 for TPA and in 24/29 for CA15/3. However, an overlap was found between carcinoma and normal tissue levels, particularly for CEA and TPA. We can conclude that the four substances studied may be markers of malignancy in breast carcinoma when nonmalignant breast tissue from the same patient is determined at the same time, whereas assays within a single, unknown breast tissue sample may be useful only in the case of ferritin and, partly, CA15/3.

scholarly journals Variability of glutathione S-transferase isoenzyme patterns in matched normal and cancer human breast tissue

1994 ◽  
Vol 304 (3) ◽  
pp. 843-848 ◽  
Author(s):  
M K Kelley ◽  
A Engqvist-Goldstein ◽  
J A Montali ◽  
J B Wheatley ◽  
D E Schmidt ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Affinity Column ◽  
Cancer Tissue ◽  
Breast Cancer Patients ◽  
Glutathione S Transferase

The determination of GST levels in blood has been proposed to a marker of tumour burden in general, whereas level of the P1 isoenzyme has been identified as a prognostic factor for breast-cancer patients receiving no adjuvant chemotherapy. Particular glutathione S-transferase (GST) isoenzymes differ in their substrate specificity, however, and their presence or absence might therefore account for the resistance of tumours to particular chemotherapeutic drugs, as already established for cultured cell lines. Determination of the GST isoenzyme profile of a cancer tissue could have prognostic value in the selection of treatment if the levels of expression/activity show a degree of variation comparable with that exhibited by actual patient responses. Using reversed-phase h.p.l.c. to quantify affinity-isolated GSTs, we have analysed full isoenzyme profiles in the first large sample of matched normal and cancer human tissues (18 breast-cancer patients). In no patients did the tumour tissues express any isoenzymes that were not found in normal breast tissue. In addition to the GSTs, another enzyme, identified as enoyl-CoA isomerase, was regularly found in breast tissue cytosol following elution from a hexyl-glutathione affinity column. In most cases, the average level of GST was substantially elevated in the cancer tissues above the levels in normal breast tissue from the same patient. Furthermore, the relative levels of the isoenzymes were substantially more variable in the cancer samples than in the normal breast tissue, providing a plausible mechanism for the well established variable response to treatment.

scholarly journals Increased epigenetic age in normal breast tissue from luminal breast cancer patients

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Erin W. Hofstatter ◽  
Steve Horvath ◽  
Disha Dalela ◽  
Piyush Gupta ◽  
Anees B. Chagpar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Luminal Breast Cancer ◽  
Breast Cancer Patients ◽  
Epigenetic Age

scholarly journals Evaluation of Antioxidant Intakes in Relation to Inflammatory Markers Expression Within the Normal Breast Tissue of Breast Cancer Patients

2016 ◽  
Vol 16 (4) ◽  
pp. 485-495 ◽  
Author(s):  
Danielle Larouche ◽  
Mirette Hanna ◽  
Sue-Ling Chang ◽  
Simon Jacob ◽  
Bernard Têtu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Postmenopausal Women ◽  
Breast Tissue ◽  
Transforming Growth Factor ◽  
Normal Breast Tissue ◽  
Inflammatory Marker ◽  
Normal Breast ◽  
Antioxidant Vitamin ◽  
Breast Cancer Patients ◽  
Marker Expression

Chronic inflammation may be a causative factor in breast cancer. One possible underlying mechanism is the generation of oxidative stress, which may favor tumorigenic processes. Antioxidant consumption may, therefore, help reduce tissue inflammation levels. However, few studies have explored this relation in breast tissue. We aimed to evaluate correlations between antioxidant (vitamin A/retinol, vitamin C, vitamin E, β-carotene, α-carotene, lycopene, lutein/zeaxanthin, β-cryptoxanthin, selenium, and zinc) intakes and protein expression levels of interleukin (IL)-6, tumor necrosis factor-α, C-reactive protein, cyclooxygenase-2, leptin, serum amyloid A1, signal transducer and activator of transcription 3, IL-8, IL-10, lactoferrin, and transforming growth factor-β measured in the normal breast tissue of 160 women diagnosed with breast cancer. Antioxidant intakes were collected using a self-administered food frequency questionnaire. Inflammation marker expression was assessed by immunohistochemistry. Correlations between antioxidant intakes and inflammatory marker expression were evaluated using Spearman’s partial correlation coefficients ( r) for all women and for premenopausal and postmenopausal women separately. After Bonferroni correction, negative correlations were observed between dietary β-tocopherol and IL-10 expression in all women combined ( r = −0.26, P = .003) and among postmenopausal women ( r = −0.39, P = .003). For all women, a negative correlation was found between total zinc intakes and IL-10 ( r = −0.26, P = .002). Among postmenopausal women, dietary selenium intake was negatively correlated with the expression of lactoferrin ( r = −0.39, P = .003). No associations were observed in premenopausal women. Our findings suggest that consumption of specific antioxidants, including β-tocopherol, zinc, and selenium, may act on the breast tissue through mechanisms affecting the expression of some inflammation markers, particularly among postmenopausal women.

The association of tissue and serum adiponectin levels with the parameters of insulin-resistance in breast cancer patients

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e22172-e22172
Author(s):  
M. Seker ◽  
F. Y. Erkal ◽  
A. Bilici ◽  
T. Salman ◽  
B. O. Ustaalioglu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Breast Tissue ◽  
Tumor Tissue ◽  
Normal Breast Tissue ◽  
Inverse Correlation ◽  
Normal Breast ◽  
The Other ◽  
Serum Adiponectin ◽  
Breast Cancer Patients

e22172 Background: Adiponectin is a novel adipocyte-secreted proteine and associated with insulin-resistant (IR) status, such as type 2 diabetes mellitus and obesity. The inverse correlation between serum adiponectin levels and breast cancer risk was previously documented. Moreover, the association of high tissue adiponectin levels with breast cancer has been recently reported. In the present study, the relationship among tumor, normal breast tissue and serum adiponectin levels, breast cancer, and the other IR parameters were evaluated. Methods: Fifty-three patients with diagnosed and histologically confirmed breast cancer were included in our study. We analyzed the correlation among the levels of normal and tumor breast tissue adiponectin and serum adiponectin levels. In addition, the association of tissue and serum adiponectin levels with the various classical risk and IR factors, such as body mass index, menopausal status and, tumor size, stage, lymph node status, hormonal status were also studied. Results: Tumor tissue adiponectin levels (56 ± 9.6 ng/ml) were similar with normal breast tissue (56 ± 10 ng/ml) (p>0.05). However, the serum adiponectin levels were significantly lower compared with both normal and tumor tissue (p<0.05). In addition, the inversely association of serum adiponectin levels with tumor tissue adiponectin levels was detected (p=0.001). The inverse correlation between T stage and tumor tissue adiponectin was found (p=0.03). The levels of serum adiponectin were significantly more higher in patients with c- erb-B2 overexpressed (p=0,008). Both nuclear and histologic grade were significantly associated with serum adiponectin levels (p=0.04,p=0.04, respectively). On the other hand, the reverse relationship between nuclear grade and, both tumor (p=0.01) and normal tissue (p=0.009) adiponectin levels was also detected. In subgroup analysis, the correlation among demographic, clinicopathologic, IR parameters, tissue and serum adiponectin levels was not found (p>0.05). Conclusions: Our results suggest that the low serum adiponectin and high normal and tumor tissue adiponectin levels detected in breast cancer patients and serum adiponectin levels inversely associated with tumor tissue adiponectin levels. No significant financial relationships to disclose.

Elevated Luteinizing Hormone in Serum, Breast Cancer Tissue, and Normal Breast Tissue from Breast Cancer Patients

2002 ◽  
Vol 76 (2) ◽  
pp. 125-130 ◽  
Author(s):  
Elvio G. Silva ◽  
Dinu Mistry ◽  
Donghui Li ◽  
Henry M. Kuerer ◽  
Edward N. Atkinson ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Luteinizing Hormone ◽  
Cancer Patients ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Breast Cancer Patients
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