Correlation of expression of pro-apoptotic proteins bax and bak with lymph node metastasis in colonic adenocarcinoma

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e15042-e15042
Author(s):  
R. A. Tracy ◽  
S. T. Chuang ◽  
J. Kim ◽  
D. Coppola
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Odds Ratio ◽  
Tumor Metastasis ◽  
Patient Survival ◽  
Tissue Microarrays ◽  
Colonic Adenocarcinoma ◽  
Node Metastasis ◽  
Apoptotic Proteins ◽  
Abc Method

e15042 Background: Colon cancer has been associated with disturbances in the regulation of apoptosis. Down regulation of Bax and Bak, members of the Bcl-2 family, are associated with tumor metastasis. Here we investigate how these two pro-apoptotic markers correlate with lymph node (LN) metastasis in colonic adenocarcinoma (CA). Methods: Paraffin-embedded sections from 100 cases of CA were constructed in tissue microarrays and subjected to immunohistochemistry (IHC) using the ABC method and antibodies for Bax and Bak. The IHC score is the product of intensity and percentage of positively stained cells, and ranged between 0 and 9. The total scores were divided into categories of “2 or less” and “3 or more.” These were than correlated with LN metastasis. Results: For Bax, 88 tumors were available while Bak was present in 100 tumors. In scoring Bax, 67 tumors had a score of ≤2 and 21 had a score of ≥3. Within the ≤2 category, 23 of 67 (34%) tumors had no LN metastases while 15 of 21 (71%) in the ≥3 category had no metastasis (p= 0.0029). For Bak staining, 23 had a score of ≤2 and 77 had a score of ≥3. Five of 23 (22%) tumors with a score ≤2 had no metastases and 35 of 77 (46%) with score ≥3 had no metastases (p= 0.0427). The LOGISTIC Procedure was used to show that a patient with a ≥3 Bax score has a 4.78 times odds ratio (95% C.I.: 1.64- 13.98) of having no metastases while a patient with a ≥3 Bak score has a 3 times odds ratio (95% C.I.: 1.01- 8.90) of having no metastases. Conclusions: We show correlation of the pro-apoptotic proteins Bax and Bak with decreased lymph node metastasis in CA. The findings suggest a role for these markers in predicting stage and patient survival in colonic adenocarcinoma. No significant financial relationships to disclose.

The association between hyperfibrinogenemia and clinicopathological factors, and the survival analysis of gastric cancer.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16547-e16547
Author(s):  
Yu Su ◽  
Xuecong Zhu ◽  
Jing Zuo ◽  
Fengling Liu ◽  
Yudong Wang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Tumor Size ◽  
Odds Ratio ◽  
Fibrinogen Level ◽  
Large Tumor ◽  
Large Tumor Size ◽  
Node Metastasis ◽  
Advanced Tumor

e16547 Background: It is reported that hyperfibrinogenemia is commonly seen in gastric cancer. This study aim to discuss the association between fibrinogen level and preoperative clinicopathological factors and to evaluate the value as a predictor of prognosis. Methods: Retrospectively reviewed the medical records and follow-up data of patients with gastric cancer who underwent curative resection from January 2011 to December 2014 at Surgery Department of the Fourth Hospital of Hebei Medical University. Fibrinogen was measured a week before the surgery. Results: A total of 248 cases were enrolled. The means±SD of fibrinogen was 3.28±1.06g/L. Fibrinogen level was higher in older adults(≥60y), advanced tumor, poorly differentiated, deep invasion, lymph node metastasis, large tumor size and in those with high CEA, platelet count, albumin, NLR and PLR,( P< 0.05). All the people were divided by the TNM staging system and found that the plasma fibrinogen was higher in stageⅡand Ⅲ (stageⅠvs. stageⅡ: 2.84±0.72g/L vs. 3.36±1.18g/L, P= 0.009;stageⅠvs. stage Ⅲ: 2.84±0.72g/L vs. 3.43±1.07g/L, P< 0.001), however, there was no difference between stageⅡand Ⅲ( P= 0.662)(our study did not enrolled stage IV patients).When patients were classified into 4 groups according to the T classification, the plasma fibrinogen level gradually increased with increasing depth of cancer invasion (one way ANOVA; P= 0.002). Yet, when the patients who have lymph nodes metastasis classified according to the N classification, the differences among them did not have statistically significance ( P= 0.333). Multivariate analysis revealed that hyperfibrinogenemia had an independent association with advanced cancer (odds ratio,2.686(1.012-7.125); P= 0.047), lymph node metastasis (odds ratio,2.012 (1.012-3.125); P= 0.035) and tumor size(odds ratio,1.949 (1.099-3.454); P= 0.022). Our study aslo suggested that the patients with hyperfibrinogenemia before surgery showed a significantly lower survival rate (Log-Rank test; P< 0.001), hyperfibrinogenemia was a independent predictor on the overall survival, which could predict worse clinical outcome. Conclusions: Hyperfibrinogenemia may be considered a useful biomarker to predict advanced tumor, lymph node metastasis and large tumor size and can be a good predictor of worse clinical outcome.

scholarly journals Fatty acid oxidation: driver of lymph node metastasis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mao Li ◽  
Hong-chun Xian ◽  
Ya-Jie Tang ◽  
Xin-hua Liang ◽  
Ya-ling Tang
Keyword(s):  
Lymph Node ◽  
Fatty Acid ◽  
Lymph Node Metastasis ◽  
Tumor Cells ◽  
Fatty Acid Oxidation ◽  
Tumor Metastasis ◽  
Cancer Metabolism ◽  
Acid Oxidation ◽  
Node Metastasis

AbstractFatty acid oxidation (FAO) is the emerging hallmark of cancer metabolism because certain tumor cells preferentially utilize fatty acids for energy. Lymph node metastasis, the most common way of tumor metastasis, is much indispensable for grasping tumor progression, formulating therapy measure and evaluating tumor prognosis. There is a plethora of studies showing different ways how tumor cells metastasize to the lymph nodes, but the role of FAO in lymph node metastasis remains largely unknown. Here, we summarize recent findings and update the current understanding that FAO may enable lymph node metastasis formation. Afterward, it will open innovative possibilities to present a distinct therapy of targeting FAO, the metabolic rewiring of cancer to terminal cancer patients.

scholarly journals Effects of Obesity on Presentation of Breast Cancer, Lymph Node Metastasis and Patient Survival: A Retrospective Review

2013 ◽  
Vol 14 (4) ◽  
pp. 2225-2229 ◽  
Author(s):  
Ahmad Kaviani ◽  
MohamadReza Neishaboury ◽  
Narjes Mohammadzadeh ◽  
Maryam Ansari-Damavandi ◽  
Khatereh Jamei
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Retrospective Review ◽  
Patient Survival ◽  
Node Metastasis

scholarly journals P-P43 Lymph Node Negative Duodenal Adenocarcinoma Is Associated with Long-term Patient Survival following Pancreaticoduodenal Resection

2021 ◽  
Vol 108 (Supplement_9) ◽  
Author(s):  
Pranav Patel ◽  
Marina Likos-Corbett ◽  
Satvinder Mudan ◽  
Amir Khan ◽  
Sacheen Kumar ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Pancreatic Resection ◽  
Patient Survival ◽  
Distal Gastrectomy ◽  
Nodal Metastasis ◽  
R0 Resection ◽  
Duodenal Adenocarcinoma ◽  
Node Metastasis

Abstract Background Duodenal adenocarcinoma (DA) is a rare gastrointestinal malignancy. Due to the low incidence of DA there is limited data reporting patient outcomes following radical pancreatic resection. Large retrospective single and multi-centre studies suggest that lymph node metastasis is an important factor for long-term patient survival following resection. The management of DA has tended to favour aggressive surgical resection with pancreaticoduodenectomy (PD), although a morbidity of up to 50% has been reported, mostly related to post-operative pancreatic fistulas. We assessed the disease-free (DFS) and overall survival (OS) in patients undergoing pancreaticoduodenectomy for DA in our institution. Methods We retrospectively analysed all patients undergoing pancreatic resection for DA at our institution between January 2009 – March 2020 inclusive. All DAs were cytologically or histologically proven prior to surgical resection following imaging review in a Hepato-pancreaticobiliary multidisciplinary team meeting. Patients underwent a Whipple’s with distal gastrectomy or pylorus preserving pancreaticoduodenectomy (PPPD) based on tumour size and location. Statistical analysis was performed by a Mann-Whitney U test using a p-value significance of 0.05 (SPSS, IBM, USA). DFS and OS curves were presented by Kaplan- Meier survival curves.  Results 19 patients underwent pancreatic resection at our institution for DA during the study period. 12 patients underwent Whipple’s with distal gastrectomy and 9 patients underwent PPPD. The overall postoperative morbidity and mortality was 37% and 5% respectively. R0 resection was achieved in 18 patients (95%). 9 patients (47%) had no nodal involvement. Median follow up was 31 months (range 1-108 months). Median DFS was 17 months but was significantly higher in patients with no nodal metastasis [p &lt; 0.001]. Median OS was 9.5 months for the whole cohort but was significantly higher in the patients with no nodal vs nodal metastasis (60 vs 17.5 months respectively) p &lt; 0.003].   Conclusions DA can be resected by PD or segmental resection. PD is favoured due to improved resection margins and overall increased patient survival, despite an increased morbidity. Our series reports comparable morbidity and mortality to the published literature for DA resected by PD. This study reports a 95% R0 resection rate for DA with a 3- and 5-year survival of 50% and 30% respectively. DFS was found to be significantly higher in patients with no nodal disease, despite predominant T4 disease. This series has identified that lymph node metastasis is one of the most important prognostic determinants of long-term patient survival. Program permission yes

scholarly journals A Case of Early Colonic Adenocarcinoma with Prominent Neutrophil Infiltrates Showing N2 Lymph Node Metastasis

2006 ◽  
Vol 18 (3) ◽  
pp. 133-136
Author(s):  
Toshiaki KUNIMURA ◽  
Masashi SATO ◽  
Syun SATO ◽  
Tomokazu HISAYUKI ◽  
Yukihiro WADA ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Colonic Adenocarcinoma ◽  
Node Metastasis

scholarly journals Atypical Histiocytoid Cells and Multinucleated Giant Cells in Fine-Needle Aspiration Cytology of the Thyroid Predict Lymph Node Metastasis of Papillary Thyroid Carcinoma

Cancers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 816 ◽  
Author(s):  
Ji Eun Choi ◽  
Ja Seong Bae ◽  
Dong-Jun Lim ◽  
So Lyung Jung ◽  
Chan Kwon Jung
Keyword(s):  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Odds Ratio ◽  
Cervical Lymph Node ◽  
Cervical Lymph Node Metastasis ◽  
Giant Cells ◽  
Needle Aspiration ◽  
Multinucleated Giant Cells ◽  
Node Metastasis ◽  
Needle Aspiration Cytology

Preoperative detection of cervical lymph node metastasis in papillary thyroid carcinoma (PTC) is crucial for determining the surgical strategy to prevent locoregional recurrence of the disease. We identified the cytological predictors of lymph node metastasis in 222 consecutive patients with PTC using fine-needle aspiration cytology (FNAC) of the thyroid. Cervical lymph node metastases occurred in 99 (44.6%) of 222 PTC patients. Lymph node metastasis was significantly associated with tumor multifocality (p = 0.003), and high cellularity (p = 0.021), atypical histiocytoid cells (p < 0.001), and multinucleated giant cells (p < 0.001) in thyroid FNAC. The BRAF V600E mutation was marginally associated with lymph node metastasis (p = 0.054). Multivariate analysis revealed that atypical histiocytoid cells (odds ratio = 2.717; p = 0.001) and multinucleated giant cells (odds ratio = 3.070; p = 0.031) were independent predictors of lymph node metastasis in patients with PTC. In a subgroup analysis of 164 patients with microcarcinomas, atypical histiocytoid cells (odds ratio = 2.761; p = 0.005) was an independent predictor of lymph node metastasis. Cytological detection of atypical histiocytoid cells and multinucleated giant cells on thyroid FNAC can be used to preoperatively predict cervical lymph node metastasis in patients with PTC.

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