Changing properties of somatic accessory and germinal cells during the amitotic/mitotic and premeiotic/meiotic transitions of spermatogenesis in Asterias vulgaris

Characteristics of encephalomyocarditis (EMC) virus-induced testicular lesions were investigated in 4- and 8-week-old BALB/c male mice after intraperitoneal (i.p.) and intratesticular (left) (i.t.) inoculation of the D variant of EMC virus (EMC-D). Apart from variation in severity and incidence, the histopathological nature of the resultant testicular lesion was similar in all infected mice, and was characterized by degeneration and necrosis of germinal cells and spermatogonia with inflammatory infiltration. Almost all the inoculated left testes of the i.t. group developed marked lesions. In general, the virus titre in the testis and incidence of testicular lesions were higher in 4-week-old mice than in 8-week-old mice. In addition, testicular lesions developed earlier and with a higher incidence in the PBS-inoculated right testis of the i.t. group than in either testis of the i.p. group of the same age.

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Absence of a nuclear androgen receptor in isolated germinal cells of rat testis

Molecular and Cellular Endocrinology ◽

10.1016/0303-7207(77)90117-4 ◽

1977 ◽

Vol 9 (2) ◽

pp. 159-167 ◽

Cited By ~ 59

Author(s):

J.A. Grootegoed ◽

M.J. Peters ◽

E. Mulder ◽

F.F.G. Rommerts ◽

H.J. Van Der Molen

Keyword(s):

Androgen Receptor ◽

Rat Testis ◽

Germinal Cells

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Echinococcus multilocularis: In vitro secretion of antigen by hybridomas from metacestode germinal cells and murine tumor cells

Experimental Parasitology ◽

10.1016/0014-4894(89)90096-9 ◽

1989 ◽

Vol 68 (2) ◽

pp. 186-191 ◽

Cited By ~ 8

Author(s):

Angela Dieckmann-Schuppert ◽

Andreas Ruppel ◽

Reinhard Burger ◽

Werner Frank

Keyword(s):

Tumor Cells ◽

Echinococcus Multilocularis ◽

Murine Tumor ◽

Germinal Cells ◽

Murine Tumor Cells

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Testicular effects of a postnatal GnRH antagonist in domestic cats

Acta Veterinaria Hungarica ◽

10.1556/004.2021.00052 ◽

2021 ◽

Keyword(s):

Gnrh Antagonist ◽

Domestic Cat ◽

Domestic Cats ◽

Low Concentrations ◽

Cellular Debris ◽

Reduced Height ◽

Faecal Samples ◽

Luminal Area ◽

Germinal Cells

Abstract The aim of this study was to describe the histological effects of two high postnatal doses of the potent third-generation GnRH antagonist, acyline in the domestic cat testicle. Secondly, the physical, endocrine, and steroidogenic findings of this pharmaceutical protocol are also reported. Twelve postnatal littermate male kittens were administered acyline in a dose of 2.2 mg/100 g SC weekly for 2 weeks (ACY; n = 6), or placebo (PL; n = 6). All the animals were followed up until puberty when they were castrated. Serial faecal samples were collected until the age of 10 weeks for testosterone (T) measurement. The kittens achieved puberty without either age (236.5 ± 19.7 vs. 221.7 ± 23.7 days) or body weight (3.05 ± 0.15 vs. 2.78 ± 0.28 kg, P > 0.05) differences between ACY and PL, respectively. Acyline suppressed faecal T concentrations for 3 weeks (P < 0.01). From the fourth week on, both groups had low concentrations up to the end of the follow-up period (P > 0.05). Histological assessment of the testes showed that ACY cats presented a reduced height of the epithelium (P < 0.01) due to the diminished number of germinal cells accompanied by an enlarged luminal area (P < 0.01) with cellular debris (P < 0.01). The immunostaining of P450c17 also appeared partially diminished in ACY testes.

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Changes in mRNA length accompany translational regulation of the somatic and testis-specific cytochrome c genes during spermatogenesis in the mouse

Development ◽

10.1242/dev.110.1.249 ◽

1990 ◽

Vol 110 (1) ◽

pp. 249-257

Author(s):

L.E. Hake ◽

A.A. Alcivar ◽

N.B. Hecht

Keyword(s):

Cytochrome C ◽

Translational Regulation ◽

Northern Blot Analysis ◽

Size Class ◽

Mrna Levels ◽

Germinal Cell ◽

Somatic Tissues ◽

Mrna Length ◽

Pachytene Spermatocytes ◽

Germinal Cells

The mouse testis contains two isotypes of cytochrome c, which differ in 14 of 104 amino acids: cytochrome cs is present in all somatic tissues and cytochrome cT is testis specific. The regulation of cytochrome cS and cytochrome cT gene expression during spermatogenesis was examined by Northern blot analysis using specific cDNA probes. Total RNA was isolated from adult tissues, enriched germinal cell populations and polysomal gradients of total testis and isolated germinal cells. Three cytochrome cS mRNAs were detected averaging 1.3 kb, 1.1 kb and 0.7 kb in all tissues examined; an additional 1.7 kb mRNA was observed in testis. Isolated germinal cells through prepuberal pachytene spermatocytes contained only the three smaller mRNAs; the 1.7 kb mRNA was enriched in round spermatids. All three smaller cytochrome cS mRNAs were present on polysomes; the 1.7 kb mRNA was non-polysomal. Cytochrome cT mRNA of 0.6-0.9 kb was detected in testis; mRNA levels were low in early spermatogonia and peaked in prepuberal pachytene spermatocytes. In adult pachytene spermatocytes, a subset of the cytochrome cT mRNAs, 0.7-0.9 kb, was present on polysomes; a shortened size class, 0.6-0.75 kb, was non-polysomal. A distinct, primarily non-polysomal, cytochrome cT 0.7 kb mRNA was present in round spermatids. These results indicate that (1) both cytochrome cS and cytochrome cT mRNAs are present in early meiotic cells, (2) a 1.7 kb cytochrome cS mRNA is post-meiotically expressed and non-polysomal and (3) cytochrome cS and cytochrome cT mRNAs are each developmentally and translationally regulated during spermatogenesis.

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