Automated Extraction and Analysis of Morphological Features for Species Identification

2009 ◽  
Vol 53 (10) ◽  
pp. 4514-4517 ◽  
Author(s):  
Laura Alcazar-Fuoli ◽  
Emilia Mellado ◽  
Ana Alastruey-Izquierdo ◽  
Manuel Cuenca-Estrella ◽  
Juan L. Rodriguez-Tudela

ABSTRACT A phylogenetic analysis was performed for 34 Aspergillus strains belonging to section Nigri. Molecular methods allowed for the correct classification into three different clades (A. niger, A. tubingensis, and A. foetidus). Correlation with in vitro itraconazole susceptibility distinguished the following three profiles: susceptible, resistant, and showing a paradoxical effect. A number of different species whose morphological features resemble those of A. niger showed unusual MICs to itraconazole that have never been described for the Aspergillus genus.


Author(s):  
В. В. Мельничук ◽  
С. С. Сорокова

Наведено результати досліджень щодо видової ідентифікації нематод виду Strongyloides papillosus (Wedl, 1856), виділених від овець з урахуванням їх морфометричних характеристик. Встановлено диференційні морфологічні особливості будови паразитичних форм гельмінтів даного виду – партеногенечних самок, виділених з тонкої кишки тварин. Визначено метричні особливості за 24 показниками у будові тіла збудника стронгілоїдозу овець Strongyloides papillosus, що підвищать ефективність його видової ідентифікації. Проведено порівняльний аналіз отриманих морфометричних показників стронгілоїдесів із загальновідомим визначником. In modern conditions of farming, the sheep industry can be one of the pеrspective for recovery and its development from the point of view of increasing the effective using of land, the level of employment of the population, providing the national sector of processing and light industry with raw materials with medicinal properties. One of the reasons that reduces the efficiency of sheep breeding is helminthic diseases, including stronhyloidosis. As a result of this invasion, the young species lose in growth and weight, changes quality of wool, sometimes the death of animals occurs. Therefore, the determination of the features of the identification of this causative agent is an actual direction of scientific research. The purpose of the work was to study the features of the species identification of parthenogenetic females Strongyloides papillosus isolated from sheep. The task of the research was to explore the morphological features of the structure of the stronhyloid species of the S. papillosus species; to establish metric species characteristics of nematodes of S. papillosus species. The research was conducted during 2016–2018 in the laboratory of the Department of Parasitology and Veterinary and Sanitary Expertise at Poltava State Agrarian Academy. The collection of nematodes was carried out by the method of complete helminthic autopsy of organs of the digestive tract of dead or slaughtered sheep that came to hand from farms of Poltava, Kyiv and Zaporizhzhia regions. As a result of parasitological research of small intestines from slaughtered sheep, a stronhyloidosis agent of the species Strongyloides papillosus (Wedl, 1856) was identified, which is represented by a parthenogenetic parasitic female with specific morphological and metric characteristics. Differential morphological features of parasitic females S. papillosus are the structure of the anterior and tail end, vulva area, uterus, and the presence of interception in the area of the esophagus transition into the intestine. To increase the effectiveness of species identification of parthenogenetic females S. papillosus, 24 metric indicators are offered. Prospects for further research are the determination of the biological qualities of Strongyloides papillosus in the seasonal aspect.


Phytotaxa ◽  
2021 ◽  
Vol 487 (3) ◽  
pp. 233-250
Author(s):  
LUCAS A.P. DAUNER ◽  
SAMANTHA C. KARUNARATHNA ◽  
SAOWALUCK TIBPROMMA ◽  
JIANCHU XU ◽  
PETER E. MORTIMER

A bioluminescent macrofungus found growing on dead wood in the mountains of Southwest China, Yunnan Province, Yulong Naxi Autonomous County, is analyzed and described. Phylogenetic analyses of the nuclear ribosomal large subunit (LSU) and internal transcribed spacer (ITS) gene regions place the fungus within the genus Roridomyces and confirm it to be a previously undescribed taxon. Morphological features support phylogenetic conclusions and include a glutinous stipe, a light yellowish-brown to beige or white pileus, and luminescent mycelium. Comprehensive descriptions, macro- and microscopic photographs, and phylogenetic trees are provided, as well as a table containing morphology and distribution of all Roridomyces taxa to aid in species identification and comparison. This is the first member of the genus Roridomyces to be identified in China.


2019 ◽  
Vol 15 (01) ◽  
pp. 14-16
Author(s):  
Rupali Charjan ◽  
N C Nandeshwar ◽  
S B Banubakode ◽  
N V Kurkure ◽  
S W Bonde

The dorsal guard hair is considered as a mammalian exoskeleton. The anatomical feature of the three concentric layers of the mammalian hair, viz., medulla, cortex, and cuticle vary considerably with different species. The characteristics features of cuticular scales and medullary pattern can be used as an important tool for species identification by using various methods for hair analysis. The morphological features of cuticle and medulla of dorsal guard hair samples of Sambar (Rusa unicolor), Nilgai/blue bull (Boselaphus tragocamelus) and Spotted deer (Axix axis), six each, were studied during post-mortem examination. The microscopic studies showed a difference in some of the cuticular scale and medullar cells among three species. Hence, a single morphological feature of hair may not be a confirmative tool for species identification but multiple morphological features can be used as a confirmative tool of species identification. This study provides pictographic details in eead of some wild species which can be used to curb criminal acts against wild animals.


2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Lindsey F. Dougherty ◽  
Jingchun Li

One of the most well-known species in the bivalve family Limidae (d’Orbigny, 1846) is the brightly coloredCtenoides scaber(Born, 1778), commonly known as the rough file clam or flame scallop. Distinguishing this bivalve from its close relative,C. mitis(Lamarck, 1807), can be difficult using only morphological features and has led to much taxonomic confusion throughout the literature. In this study, morphological characters were compared to a molecular phylogeny constructed using three genes (COI, 28S, and H3) in order to differentiateC. scaberandC. mitis.The phylogeny recovered two well-supported clades that differ significantly in shell rib numbers, but not tentacle colors. The two species were then placed in a larger phylogenetic context of the Limidae family, which revealed the need for further systematic revision across genera. As these bivalves are popular in aquaria, cannot be tank-raised, and have been overcollected in the past, proper species identification is important for assessing sustainable collection practices.


2021 ◽  
Vol 95 ◽  
Author(s):  
M. Musah-Eroje ◽  
L. Burton ◽  
J.M. Behnke

Abstract The spicules of male parasitic nematodes are key morphological features, which vary between species in shape and length and are used often for species identification. However, little is known about spicules and particularly if/how their length varies during growth. We first assessed the degree of variation in spicule length of male Heligmosomoides bakeri 21 days post infection (PI), and then in two follow-up experiments measured spicule lengths at half daily/daily intervals between days 6 and 14 PI. Mean spicule length in 21-day worms was 0.518 mm with a range of 94 μm, and variation between the two spicules of individual worms from 2 to 32 μm. Spicules were first detectable on day 6–6.5, after which their lengths increased until day 7 PI (mean = 0.61 and 0.59). This was followed by significant contraction, initially relatively quickly over the following 48 h and then more slowly over a longer period, stabilizing by days 10–14, with only minor further reduction in length. We conclude that the length of spicules varies significantly over the first few days after they have formed, and, consequently, the age of worms is an important factor for consideration when spicule lengths are measured for experimental/diagnostic or taxonomical purposes.


2007 ◽  
Vol 52 (2) ◽  
pp. 135-145 ◽  
Author(s):  
A. Poulíčková ◽  
Z. Žižka ◽  
P. Hašler ◽  
O. Benada

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