Bacterial Fish Diseases and Molecular Tools for Bacterial Fish Pathogens Detection

Author(s):  
Ana Roque ◽  
Sonia Soto-Rodr√≠guez ◽  
Bruno Gomez-Gil
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Arfiandi Jr ◽  
Reiny A. Tumbol

This study aimed to identify the type of pathogenic bacteria that attack tilapia (Oreochromis niloticus) cultivated in Dimembe District, North Minahasa Regency in 2019. The benefit of this research were to obtain information about the types of bacteria in tilapia (Oreochromis niloticus), so prevention efforts can be made or treatment of the disease. Tilapia as a research sample obtained from existing cultivation sites in the District of North Minahasa Dimembe, North Sulawesi Province. From each location 5-10 individuals were taken with a size of 5-12 cm. The samples were then taken to the laboratory for bacterial isolation. The media for growing bacteria was TSA (Tryptic Soya Agar). The results obtained were adjusted to the Bacterial Fish Pathogens identification book. The data obtained were analyzed descriptively through the presentation of tables and figures. Research results found the bacteria that infected tilapia in North Minahasa Regency including Aeromonas sp, Plesiomonas sp, Flavobacterium sp and Enterobacter sp. Regular and continuous monitoring of fish diseases is needed to monitor the spread of fish diseases, especially quarantine fish diseases in North Minahasa Regency.


This study concentrated on the assessment of the prevailing parasitic fish diseases in some marine fishes at Ismailia province and how to control the infestation using microalgae. This study was carried out on 1080 pre-mature fish (360 D. labrax (225±25 g) and 360 S aurata (150±25 g) and 360 M. cephalus (125±25 g) collected from similar ponds of studies to be examined at the end of treatment. In addition to that we followed non-treated fish (1080 premature). The infested fish showed dark colour and respiratory signs. Post mortem lesions were a presence of congestion or paleness and destruction of gill filaments. The total prevalence of infestation was the total prevalence of parasitic infection of non-treated fishes was 45.83 %. The highest percentage was in D. labrax 56.94 % followed by S. aurata 47.22%, the lowest percentage in M. cephalus 33.33. The total prevalence of parasitic infection in premature treated with 2 g algae was 28.79%, followed by 3 g algae was 23.60 %, while the lowest percentage with 5 g algae was 20.37 % respectively. The detected species of parasites were protozoal parasites, Amyloodinium ocellatum and Riboscyphidia in additions of marine monogenea, Lamellodiscus diplodicus isolated from D Labrex, Mugil Cephalus and S aurata. The present study concluded that, the use of microalgae instead of fish meal decreased parasitic infestation in marine fish. The histopathological alteration of natural infested examined fishes was also recorded.


2019 ◽  
Vol 14 (5) ◽  
pp. 405-420 ◽  
Author(s):  
Eduardo Alvarado-Ortiz ◽  
Miguel Á. Sarabia-Sánchez ◽  
Alejandro García-Carrancá

Cancer Stem Cells (CSC) generally constitute a minor cellular population within tumors that exhibits some capacities of normal Stem Cells (SC). The existence of CSC, able to self-renew and differentiate, influences central aspects of tumor biology, in part because they can continue tumor growth, give rise to metastasis, and acquire drug and radioresistance, which open new avenues for therapeutics. It is well known that SC constantly interacts with their niche, which includes mesenchymal cells, extracellular ligands, and the Extra Cellular Matrix (ECM). These interactions regularly lead to homeostasis and maintenance of SC characteristics. However, the exact participation of each of these components for CSC maintenance is not clear, as they appear to be context- or cell-specific. In the recent past, surface cellular markers have been fundamental molecular tools for identifying CSC and distinguishing them from other tumor cells. Importantly, some of these cellular markers have been shown to possess functional roles that affect central aspects of CSC. Likewise, some of these markers can participate in regulating the interaction of CSC with their niche, particularly the ECM. We focused this review on the molecular mechanisms of surface cellular markers commonly employed to identify CSC, highlighting the signaling pathways and mechanisms involved in CSC-ECM interactions, through each of the cellular markers commonly used in the study of CSC, such as CD44, CD133, CD49f, CD24, CXCR4, and LGR5. Their presence does not necessarily implicate them in CSC biology.


2015 ◽  
Vol 2015 (10) ◽  
pp. 2825-2826 ◽  
Author(s):  
Vikram Kapoor ◽  
Xuan Li ◽  
Christopher A Impellitteri ◽  
Kartik Chandran ◽  
Jorge W Santo Domingo

2018 ◽  
Vol 36 (1) ◽  
pp. 050-056 ◽  
Author(s):  
Aladdin Hamawieh ◽  
◽  
Fida Alo ◽  
Seid Ahmed ◽  
◽  
...  

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