Storage of Karyotyped Voucher Specimens and their Molecular Identifi cation

2015 ◽  
pp. 11-20
Author(s):  
A Dettai ◽  
P Pruvost
Keyword(s):  
Voucher Specimens

Halyomorpha picus (Hemiptera: Heteroptera: Pentatomidae): first confirmed record from Pakistan and two new junior synonyms

Zootaxa ◽  
2021 ◽  
Vol 5060 (3) ◽  
pp. 429-438
Author(s):  
PETR KMENT ◽  
S. SALINI ◽  
ZUBAIR AHMED
Keyword(s):  
Invasive Species ◽  
North Africa ◽  
Male Genitalia ◽  
Morphological Variability ◽  
Female Genitalia ◽  
Voucher Specimens ◽  
Heteroptera Pentatomidae

We provide the first confirmed record of Halyomorpha picus (Fabricius, 1794) (Hemiptera: Heteroptera: Pentatomoidea: Pentatomidae: Pentatominae: Cappaeini) from Pakistan: Islamabad Capital Territory, and provide habitus photographs and electron scanning micrographs of the male genitalia of the voucher specimens. Two species previously described from Pakistan are found to fit within the morphological variability of H. picus and the following two junior subjective synonyms are proposed: Halyomorpha picus (Fabricius, 1794) = Halyomorpha punjabensis Ahmad & Kamaluddin, 1977, syn. nov., = Halyomorpha azhari Ahmad & Zaidi, 1989, syn. nov. The record of Halyomorpha scutellata Distant, 1879, from Pakistan by Sharif et al. (2020) is based on misidentification of a species of Neohalys Ahmad & Perveen, 1982 (Pentatominae: Halyini) and excluded from Pakistan fauna. Based on the analysis of female genitalia figures provided by Gadalla (2004), the record of H. picus from Egypt represents a misidentification of H. halys Stål, 1855, which extends the distribution of that invasive species to North Africa.  

scholarly journals The application of life history information to the conservation management of Chrysoritis butterflies (Lepidoptera: Lycaenidae) in South Africa

Koedoe ◽  
2004 ◽  
Vol 47 (1) ◽  
Author(s):  
R.F. Terblanche ◽  
H. Van Hamburg
Keyword(s):  
South Africa ◽  
Life History ◽  
Life Histories ◽  
Host Plants ◽  
Conservation Management ◽  
Aesthetic Value ◽  
Wing Patterns ◽  
In The Wild ◽  
History Of ◽  
Voucher Specimens

Due to their intricate life histories and the unique wing patterns and colouring the butterflies of the genus Chrysoritis are of significant conservation and aesthetic value. Thisoverview probes into practical examples of butterfly life history research applicable to environmental management of this relatively well-known invertebrate group in South Africa. Despite the pioneer work on life histories of Chrysoritis in the past, more should be done to understand the life history of the butterflies in the wild, especially their natural host plants and the behaviour of adults and larvae. A system of voucher specimens of host plants should be introduced in South Africa. Although various host plant species in nature are used by the members of Chrysoritis, including the Chrysoritis chrysaor group, the choice of these in nature by each species is significant for conservation management and in the case of Chrysoritis aureus perhaps even as a specific characteristic.A revision of the ant genus Crematogaster will benefit the conservation management of Chrysoritis species since some of these ant species may consist of a number of specieswith much more restricted distributions than previously thought. Rigorous quantified tudies of population dynamics of Chrysoritis butterflies are absent and the introductionof such studies will benefit conservation management of these localised butterflies extensively.

Checklist of the endemic vascular plants of Paraguay

Phytotaxa ◽  
2018 ◽  
Vol 384 (1) ◽  
pp. 1
Author(s):  
MARÍA DEL CARMEN PEÑA CHOCARRO ◽  
JUANA DE EGEA
Keyword(s):  
Vascular Plants ◽  
Primary Data ◽  
Endemic Plants ◽  
Voucher Specimens ◽  
Herbarium Collections ◽  
Endemic Flora

We present a list of endemic plants of Paraguay, which includes 374 taxa from 52 families and 162 genera based on the revision of primary data (herbarium collections). Synonyms, habit, distribution in Paraguay and all the voucher specimens seen or cited in recent bibliographies or in the consulted databases are provided for each taxon. A brief analysis of the diversity and importance of this endemic flora is presented. A list of excluded species, which were considered as endemics in previous publications, is also included.

Bursaphelenchus rufipennis n. sp. (Nematoda: Parasitaphelenchinae) and redescription of Ektaphelenchus obtusus (Nematoda: Ektaphelenchinae), associates from nematangia on the hind wings of Dendroctonus rufipennis (Coleoptera: Scolytidae)

Nematology ◽  
2008 ◽  
Vol 10 (6) ◽  
pp. 925-955 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Yasmin J. Cardoza ◽  
Weimin Ye ◽  
Kenneth F. Raffa ◽  
...  
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Morphological Examination ◽  
Dendroctonus Rufipennis ◽  
Lateral Field ◽  
The Past ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Voucher Specimens ◽  
Tail Tip

Abstract Two species of aphelench, Bursaphelenchus rufipennis n. sp. and Ektaphelenchus obtusus, were isolated from the 'nematangia', cocoon-like structures found at the base of the hind wings of Dendroctonus rufipennis. The nematangia contained adult females of E. obtusus and the dauer juveniles of B. rufipennis n. sp. Only B. rufipennis n. sp. could be cultured on Monilinia fructicola on LGPDA (lactic acid-treated, glycerol-supplemented, potato dextrose agar). The new species of Bursaphelenchus is described and figured and some additional morphological characters are ascribed to E. obtusus, E. josephi, E. sandiaensis, E. smaelus (= E. prolobos) and E. terebranus after examination of type and/or voucher specimens. Bursaphelenchus rufipennis n. sp. has an adult body length of ca 500-1000 μm, medium a ratios (ca 25-38 for females and ca 30-40 for males), b ratios of ca 8-13 (female) and 7-11 (male), c ratios of ca 15-22 (female and male), c′ ratios of ca 3-4 (female) and ca 2-3 (male), and is characterised by three incisures in the lateral field, mitten-shaped spicules and a conical female tail that curves ventrally and possesses a variable tail tip. The new species is morphologically closest to B. corneolus, B. curvicaudatus, B. gerberae, B. paracorneolus and B. talonus. Morphological examination of type and/or voucher specimens of five Ektaphelenchus species revealed coarse transverse body annulation and three pairs of male caudal papillae (except for the two species where males are not described). Clear typological differences were observed among these five Ektaphelenchus species in the structure of the lip region, presence/absence of stylet knobs and male spicule morphology. Although these characters have not been consistently documented in the past, they may be diagnostic for species in the genus. Molecular phylogenetic analysis based on SSU and D2/D3 LSU sequences revealed that B. rufipennis n. sp. was closest to B. paracorneolus and that E. obtusus was closest to species of Ektaphelenchoides and a Cryptaphelenchus sp.

Experimental studies on Lecanora rupicola (L.) Zahlbr.: chemical and microscopical investigations of the mycobiont and re-synthesis stages

2006 ◽  
Vol 38 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Georg BRUNAUER ◽  
Armin HAGER ◽  
Wolf Dietrich KRAUTGARTNER ◽  
Roman TÜRK ◽  
Elfie STOCKER-WÖRGÖTTER
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Sequence Data ◽  
Experimental Studies ◽  
Culture Conditions ◽  
Voucher Specimen ◽  
Dna Sequence Data ◽  
Standard Culture ◽  
Voucher Specimens ◽  
Scanning Electron

Culture experiments that trigger the axenically grown mycobionts of Lecanora rupicola to produce the polyketide chemosyndrome typical of the naturally grown lichen are reported. This chemosyndrome comprises lecanoric, haematommic and orsellinic acids, sordidone, eugenitol and atranorin, all of which were hardly produced under standard culture conditions. The only exception was arthothelin that was only present in the voucher specimen. It has been shown that almost the complete acetyl-polymalonyl-pathway leading to depsides and chromones can be induced in culture, but apparently not the xanthones. The mycobiont was also successfully re-synthesized with its original photobiont, as confirmed by Scanning Electron Microscope studies (SEM). Cultures of the resynthesised lichen biosynthesized additional satellite substances, which were not detected either in the voucher specimens or in the aposymbiontically (without the photobiont) grown mycobiont cultures. The identity of cultured mycobionts of L. rupicola was confirmed by comparing ITS-DNA-sequence data from the original lichen with publicly available (GeneBank) sequences of that species.

CHROMOSOME NUMBERS AND TAXONOMIC NOTES ON NORTHERN GRASSES: II. TRIBE FESTUCEAE

1960 ◽  
Vol 38 (2) ◽  
pp. 117-131 ◽  
Author(s):  
Wray M. Bowden
Keyword(s):  
Chromosome Numbers ◽  
Somatic Chromosome ◽  
Bromus Tectorum ◽  
Dactylis Glomerata ◽  
Bromus Inermis ◽  
Festuca Rubra ◽  
Lolium Rigidum ◽  
Voucher Specimens ◽  
Dactylis Glomerata L ◽  
Somatic Chromosome Numbers

Chromosome numbers and voucher specimens are recorded for some collections of the tribe FESTUCEAE Dumort., mainly collected in Canada. The somatic chromosome numbers of the following are recorded: (1a) Arctagrostis arundinacea (Trin.) Beal var. arundinacea, 2n = 28, 2n = 29, and 2n = 30; (1b) Arctagrostis arundinacea (Trin.) Beal var. crassispica Bowden, 2n = 56; (1c) Arctagrostis latifolia (R.Br.) Griseb., 2n = 56. (2) Beckmannia syzigachne (Steud.) Fern., 2n = 14. (3) Brachyelytrum erectum (Schreb.) Beauv., two vars., 2n = 22. (4a) Bromus ciliatus L., 2n = 14; (4b) Bromus inermis Leyss., two subspecies and two vars., 2n = 56; (4e) Bromus kalmii A. Gray, 2n = 14; (4d) Bromus porteri (Coult.) Nash, 2n = 14; (4c) Bromus tectorum L., 2n = 14. (5) Catabrosa aquatica (L.) Beauv. var. laurentiana Fern., 2n = 20. (6) Dactylis glomerata L., 2n = 28. (7) Distichlisstricta (Torr.) Rydb., 2n = 40. (8a) Dupontiafisheri R.Br, subsp. fisheri, 2n = 132; (8b) Dupontia fisheri R.Br, subsp. psilosantha (Rupr.) Hultén, 2n = 44. (9) Eremopoa persica (Trin.) Roshev., 2n = 28. (10a) Festuca altaica Trin., 2n = 28; (10b) Festuca baffinensis Polunin, 2n = 28; (10c) Festuca brachyphylla Schultes, 2n = 42 and one collection, 2n = 44; (10d) Festuca elatior L., 2n = 14; (10e) Festuca obtusa Bieler, 2n = 42; (10f) Festuca prolifera (Piper) Fern. var. lasiolepis Fern., 2n = 50; (10g) Festuca rubra L., 2n = 42; (10h) Festuca saximontana Rydb., 2n = 42; (10i)Festuca scabrella Torr. ex Hook., two vars., 2n = 56 and 2n = 28. (11a) Glyceria borealis (Nash) Batchelder, 2n = 20; (11b) Glyceria canadensis (Michx.) Trin., 2n = 60; (11c) Glyceria grandis S. Wats., 2n = 20; (11d) Glyceria melicaria (Michx.) Hubb., 2n = 40; (11e) Glyceria pulchella (Nash) K. Schum., 2n = 20; (11f) Glyceria striata (Lam.) Hitchc, 2n = 20; (11g) Glyceria × gatineauensis Bowden (G. melicaria × G. striata), 2n = 30; (11h)Glyceria × ottawensis Bowden (G. canadensis × G. striata), three nothomorphs, 2n = 42, 2n = 46, and 2n = 48. (12) Lolium rigidum Gaud., 2n = 14. (13a) Phippsiaalgida (Sol.) R.Br., 2n = 28; (13b) Phippsia concinna (Th. Fries) Lindeb., 2n = 28. (14) Pleuropogon sabinei R.Br., 2n = 42. (15) Schizachnepurpurascens (Torr.) Swallen, 2n = 20. (16) Torreyochloa fernaldii (Hitchc.) Church, 2n = 14.

Type Specimens vs. Voucher Specimens

1975 ◽  
Vol 24 (1) ◽  
pp. 110 ◽  
Author(s):  
Wm. H. Robinson
Keyword(s):  
Type Specimens ◽  
Voucher Specimens

scholarly journals Flora y Vegetación de las zonas núcleo de la Reserva de la Biosfera Mariposa Monarca, México

2017 ◽  
pp. 43
Author(s):  
Guadalupe Cornejo-Tenorio ◽  
Alejandro Casas ◽  
Berenice Farfán ◽  
José Luis Villaseñor ◽  
Guillermo Ibarra-Manríquez
Keyword(s):  
Cloud Forest ◽  
Vascular Plant ◽  
Coniferous Forest ◽  
Biosphere Reserve ◽  
Field Work ◽  
Monarch Butterfly ◽  
Oak Forests ◽  
Vegetation Types ◽  
Voucher Specimens ◽  
One Year

A study of the flora and vegetation physiognomy was conducted at the core zones of the Monarch Butterfly Biosphere Reserve, in the states of México and Michoacán. Collection of plant specimens was carried out du ring one year of field work and this activity was supplemented by a review of voucher specimens previously collected in the area and deposited at the Mexican herbaria IEB, EBUM, and MEXU. A total of 423 vascular plant species including 32 infraespecific taxa and grouped in 86 families and 244 genera were identified, Families with the largest species richness were Asteraceae (103), Lamiaceae (21), Fabaceae (17), and Scrophulariaceae (17).The most speciose genera were Salvia (13), Stevia (8), and Ageratina (7). Mexican endemic species comprised 135 (31.9%) species, which included 13 infrae spec ific taxa; 40 of these species belong to Asteraceae. Herbs were represented by 326 species, followed by shrubs (5 1) and trees (33). The main vegetation types recognized were coniferous forest (including combinations of fir, pine, and oak forests), cloud forest, anthropogenic grass land, and Quercus forest.

scholarly journals Herbaria Mundi: An image centric approach to manipulation of specimens from multiple collections

2020 ◽  
Vol 4 ◽  
Author(s):  
Roger Hyam
Keyword(s):  
User Interface ◽  
Proof Of Concept ◽  
Virus Spread ◽  
Biodiversity Crisis ◽  
International Image ◽  
Voucher Specimens ◽  
Tropical Areas ◽  
Concept Application ◽  
Extensive Reference ◽  
As If

Addressing the challenges of the Climate Emergency and the Biodiversity Crisis requires us to understand how the world's vegetation is changing. This is not a trivial task,especially in highly diverse tropical areas. Frequently, the only way to characterise vegetation is to make voucher specimens of the plants present and identify them later in the lab. Fortunately there are extensive reference collections carefully curated in herbaria. Unfortunately specimens and expertise are dispersed over hundreds of different herbaria spread across many countries. It would be more efficient if scientists could see and manipulate specimens independently of where they are stored, especially as travel becomes more difficult due to both the need to reduce carbon emissions and virus spread. Herbaria Mundi is an application demonstrating how how this can be achieved using CETAF Specimen IDs and IIIF (International Image Interoperability Framework). The application mimics the way a botanist might work in a physical herbarium by enabling the gathering of specimens into groups and side-by-side comparison of specimens, but differs in that one can search for and manipulate specimens in multiple herbaria as if they were stored in a single collection. Voucher specimens are often not added to herbaria mainly because they do not add enough value to material already in the collections. Herbaria Mundi therefore includes the ability for researchers to upload specimen images to Zenodo and for them to appear in the user interface as if they were in an institutional herbarium. This proof of concept application is being developed as part of Task 4.3 of the Synthesys+ project funded by the European Commission. Two things need to occur to take this concept into production. More herbaria need to adopt the use of CETAF specimen identifiers and the IIIF image API. Botanists need to prioritise which features they would like developed first. More herbaria need to adopt the use of CETAF specimen identifiers and the IIIF image API. Botanists need to prioritise which features they would like developed first. The poster will be a catalyst to discuss how these things can be achieved.

scholarly journals Checklist and practical identification key for the cichlid fishes (Cichliformes: Cichlidae) of the La Plata drainage in Bolivia, including three new geographical records

2019 ◽  
Author(s):  
Pascal I. Hablützel ◽  
Robert B. Huanto
Keyword(s):  
Fish Species ◽  
Cichlid Fish ◽  
Identification Key ◽  
Museum Collections ◽  
Additional Species ◽  
Cichlid Fishes ◽  
La Plata ◽  
Bolivian Amazon ◽  
Voucher Specimens ◽  
Historia Natural

AbstractIn comparison with the Bolivian Amazon, the ichthyofauna of the La Plata drainage of Bolivia received relatively little attention historically. Until now, 14 species of cichlid fish have been registered from this area. After an exhaustive review of museum collections (Museo de Historia Natural Noel Kempff Mercado y Colección Boliviana de Fauna), we can report three additional species: Astronotus crassipinnis (Heckel, 1840), Mesonauta festivus (Heckel, 1840) and Satanoperca pappaterra (Heckel, 1840). Four other species, which have been listed in previous publications, can be confirmed for the La Plata drainage of Bolivia based on the examination of voucher specimens: Aequidens plagiozonatus Kullander, 1984, Apistogramma commbrae (Regan, 1906), A. trifasciata (Eigenmann & Kennedy, 1903) and Crenicichla vittata Heckel, 1840. As such, 16 of the 17 species can be referenced with voucher specimens in museum collections. We also provide an identification key for the cichlid fish species of the study area.

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