scholarly journals Role of the 3′ Untranslated Region in Post-Transcriptional Regulation of Interleukin-10 Gene Expression. † 1297

1997 ◽  
Vol 41 ◽  
pp. 219-219
Author(s):  
Kurt R. Schibler ◽  
Trong V. Le
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Untranslated Region ◽  
Interleukin 10 ◽  
Post Transcriptional Regulation

scholarly journals The Role of the 3' Untranslated Region in the Post-Transcriptional Regulation of KLF6 Gene Expression in Hepatocellular Carcinoma

Cancers ◽  
2013 ◽  
Vol 6 (1) ◽  
pp. 28-41 ◽  
Author(s):  
Thoria Diab ◽  
Naima Hanoun ◽  
Christophe Bureau ◽  
Camille Christol ◽  
Louis Buscail ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatocellular Carcinoma ◽  
Transcriptional Regulation ◽  
Untranslated Region ◽  
Post Transcriptional Regulation

scholarly journals Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Marie-Christine Carpentier ◽  
Cécile Bousquet-Antonelli ◽  
Rémy Merret
Keyword(s):  
Gene Expression ◽  
Quality Control ◽  
Transcriptional Regulation ◽  
High Throughput ◽  
Library Preparation ◽  
Working Day ◽  
Set Up ◽  
Post Transcriptional Regulation ◽  
Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

scholarly journals Abundance of hepatic metallothionein mRNA is increased by protein-synthesis inhibitors. Evidence for transcriptional activation and post-transcriptional regulation

1991 ◽  
Vol 273 (1) ◽  
pp. 185-188 ◽  
Author(s):  
C C McCormick ◽  
L M Salati ◽  
A G Goodridge
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Transcriptional Regulation ◽  
Transcriptional Activation ◽  
Actinomycin D ◽  
Protein Synthesis Inhibitors ◽  
Mrna Accumulation ◽  
Hepatic Metallothionein ◽  
Post Transcriptional Regulation

Ongoing protein synthesis is a prerequisite in the expression of some genes. We studied the effect of various protein synthesis inhibitors on the expression of the avian metallothionein (MT) gene. Chicken embryonic hepatocytes in culture were exposed to various concentrations of cycloheximide, puromycin and pactamycin. At concentrations which decreased total protein synthesis by about 90% each inhibitor increased MT mRNA accumulation approx. 5-fold at 9 h of incubation. Incubation with puromycin or zinc for 2 h markedly increased the rate of MT gene transcription. Estimates of the half-life of MT mRNA by using actinomycin D suggested for cycloheximide, but not puromycin, decreased the decay rate of MT mRNA. These data suggest the potential for post-transcriptional regulation of the avian MT gene. We conclude that different antibiotics increase the accumulation of hepatocyte MT mRNA by different mechanisms and that the possibility of multiple mechanisms should be considered in other studies of the role of protein synthesis in gene expression.

scholarly journals The perplexities of ZC3H12A self-mRNA regulation

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Mateusz Wawro ◽  
Jakub Kochan ◽  
Aneta Kasza
Keyword(s):  
Gene Expression ◽  
Biological Activity ◽  
Transcriptional Regulation ◽  
Untranslated Region ◽  
Regulation Of Gene Expression ◽  
Loop Structure ◽  
Stem Loop ◽  
Mrna Regulation ◽  
Stem Loop Structure ◽  
Post Transcriptional Regulation

The mechanisms regulating transcripts turnover are key processes in the regulation of gene expression. The list of proteins involved in mRNAs degradation is still growing, however, the details of RNase-mRNAs interaction are not fully understood. ZC3H12A is a recently discovered inflammation-related RNase engaged in the control of proinflammatory cytokines transcripts turnover. ZC3H12A regulates also its own transcript half-live. We studied the details of this regulation. Our results confirm the importance of the 3’UTR in ZC3H12A-dependent ZC3H12A mRNA degradations. We compared mouse and human stem‑loop structures present in this region and discovered that human conserved stem-loop structure is not sufficient for ZC3H12A-dependent degradation. However, this structure is important for ZC3H12A mRNA post-transcriptional regulation. Our studies emphasize the importance of surroundings of the identified stem-loop structure for its biological activity. Removing of this region together with stem-loop structure greatly inhibits ZC3H12A regulation of the investigated 3’-untranslated region (3’UTR).

scholarly journals Unravelling HP1 functions: post-transcriptional regulation of stem cell fate

Chromosoma ◽  
2021 ◽  
Author(s):  
Assunta Maria Casale ◽  
Ugo Cappucci ◽  
Lucia Piacentini
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Stem Cell ◽  
Cell Fate ◽  
Regulation Of Gene Expression ◽  
Telomere Maintenance ◽  
Chromosomal Protein ◽  
Heterochromatin Protein 1 ◽  
Post Transcriptional Regulation

AbstractHeterochromatin protein 1 (HP1) is a non-histone chromosomal protein first identified in Drosophila as a major component of constitutive heterochromatin, required for stable epigenetic gene silencing in many species including humans. Over the years, several studies have highlighted additional roles of HP1 in different cellular processes including telomere maintenance, DNA replication and repair, chromosome segregation and, surprisingly, positive regulation of gene expression. In this review, we briefly summarize past research and recent results supporting the unexpected and emerging role of HP1 in activating gene expression. In particular, we discuss the role of HP1 in post-transcriptional regulation of mRNA processing because it has proved decisive in the control of germline stem cells homeostasis in Drosophila and has certainly added a new dimension to our understanding on HP1 targeting and functions in epigenetic regulation of stem cell behaviour.

scholarly journals Role of FAST Kinase Domains 3 (FASTKD3) in Post-transcriptional Regulation of Mitochondrial Gene Expression

2016 ◽  
Vol 291 (50) ◽  
pp. 25877-25887 ◽  
Author(s):  
Erik Boehm ◽  
María Zornoza ◽  
Alexis A. Jourdain ◽  
Aitor Delmiro Magdalena ◽  
Inés García-Consuegra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Mitochondrial Gene ◽  
Mitochondrial Gene Expression ◽  
Post Transcriptional Regulation
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