scholarly journals Chorionic Gonadotropin β-Subunit Gene Expression in the Marmoset Pituitary Is Controlled by Steroidogenic Factor 1, Early Growth Response Protein 1, and Pituitary Homeobox Factor 1

Endocrinology ◽  
2007 ◽  
Vol 148 (12) ◽  
pp. 6062-6072 ◽  
Author(s):  
Alexander Henke ◽  
C. Marc Luetjens ◽  
Manuela Simoni ◽  
Jörg Gromoll
Keyword(s):  
Gene Expression ◽  
Chorionic Gonadotropin ◽  
Growth Response ◽  
Early Growth ◽  
Β Subunit ◽  
Subunit Gene ◽  
Steroidogenic Factor 1 ◽  
Early Growth Response

scholarly journals p300 Regulates the Synergy of Steroidogenic Factor-1 and Early Growth Response-1 in Activating Luteinizing Hormone-β Subunit Gene

2003 ◽  
Vol 279 (9) ◽  
pp. 7832-7839 ◽  
Author(s):  
Jean-François Mouillet ◽  
Christina Sonnenberg-Hirche ◽  
Xiaomei Yan ◽  
Yoel Sadovsky
Keyword(s):  
Luteinizing Hormone ◽  
Growth Response ◽  
Early Growth ◽  
Β Subunit ◽  
Subunit Gene ◽  
Steroidogenic Factor 1 ◽  
Early Growth Response ◽  
Early Growth Response 1

scholarly journals The Protein Kinase C System Acts through the Early Growth Response Protein 1 to Increase LHβ Gene Expression in Synergy with Steroidogenic Factor-1

1999 ◽  
Vol 13 (1) ◽  
pp. 106-116 ◽  
Author(s):  
Lisa M. Halvorson ◽  
Ursula B. Kaiser ◽  
William W. Chin
Keyword(s):  
Gene Expression ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Growth Response ◽  
Gene Promoter ◽  
Early Growth ◽  
Mrna Levels ◽  
Steroidogenic Factor 1 ◽  
Early Growth Response ◽  
Kinase C

Abstract Expression of the LHβ gene has been shown to be modulated by both the orphan nuclear receptor, steroidogenic factor-1 (SF-1), and the early growth response protein 1, Egr-1. It is also well known that LHβ mRNA levels are increased after hormonal activation of the protein kinase C (PKC) signaling system, for example by GnRH; however, the mechanisms by which the PKC system exerts this effect has not been fully characterized. By transient transfection of the GH3 cell line, we demonstrate that activation of the PKC system with the phorbol ester, phorbol 12-myristate 13-acetate (PMA), increases activity of region −207/+5 of the rat LHβ gene promoter (∼2-fold) and markedly augments SF-1-induced stimulation (95-fold in the presence of both factors vs. 13-fold for SF-1 alone). Mutation of the two previously identified Egr-1 sites not only prevents Egr-1 effects on the LHβ gene promoter, but also eliminates the synergistic response to PMA and SF-1 together, findings that were confirmed in a longer construct spanning region −797/+5. In the gonadotrope-derived cell line,α T3–1, these mutations eliminate the GnRH responsiveness of the− 207/+5 LHβ promoter construct. We next show that PMA treatment (GH3 and αT3–1 cells) or GnRH treatment (αT3–1 cells) induces expression of Egr-1, as detected by Egr-1 interaction with Egr-1 DNA-binding sites in the rat LHβ gene promoter sequence. Furthermore, we demonstrate that PMA increases steady-state Egr-1 mRNA levels via increased Egr-1 transcription. We conclude that PMA-induced stimulation of LHβ gene expression is achieved, at least in part, by induction of Egr-1 expression.

scholarly journals Induction of Early Growth Response Protein-1 Gene Expression in the Rat Ovary in Response to an Ovulatory Dose of Human Chorionic Gonadotropin*

Endocrinology ◽  
2000 ◽  
Vol 141 (7) ◽  
pp. 2385-2391 ◽  
Author(s):  
Lawrence L. Espey ◽  
Takeshi Ujioka ◽  
Darryl L. Russell ◽  
Molly Skelsey ◽  
Bogdan Vladu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Growth Response ◽  
Early Growth ◽  
Early Growth Response ◽  
Rat Ovary

scholarly journals Early Growth Response gene 1 (Egr-1) regulates HSV-1 ICP4 and ICP22 gene expression

Cell Research ◽  
2007 ◽  
Vol 17 (6) ◽  
pp. 546-555 ◽  
Author(s):  
Gautam R Bedadala ◽  
Rajeswara C Pinnoji ◽  
Shao-Chung V Hsia
Keyword(s):  
Gene Expression ◽  
Growth Response ◽  
Early Growth ◽  
Response Gene ◽  
Early Growth Response ◽  
Early Growth Response Gene ◽  
Hsv 1
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