scholarly journals Gonadotropin-Inhibitory Hormone Neurons Interact Directly with Gonadotropin-Releasing Hormone-I and -II Neurons in European Starling Brain

Endocrinology ◽  
2007 ◽  
Vol 149 (1) ◽  
pp. 268-278 ◽  
Author(s):  
Takayoshi Ubuka ◽  
Stephanie Kim ◽  
Yu-chi Huang ◽  
Jessica Reid ◽  
Jennifer Jiang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Neuronal Activity ◽  
Cellular Localization ◽  
Neuronal Cell ◽  
European Starling ◽  
Inhibitory System ◽  
Axon Terminals ◽  
Neuronal Cell Bodies ◽  
Gonadotropin Inhibitory Hormone

Gonadotropin-inhibitory hormone (GnIH) is a hypothalamic dodecapeptide (SIKPSAYLPLRF-NH2) that directly inhibits gonadotropin synthesis and release from quail pituitary. The action of GnIH is mediated by a novel G-protein coupled receptor. This gonadotropin-inhibitory system may be widespread in vertebrates, at least birds and mammals. In these higher vertebrates, histological evidence suggests contact of GnIH immunoreactive axon terminals with GnRH neurons, thus indicating direct regulation of GnRH neuronal activity by GnIH. In this study we investigated the interaction of GnIH and GnRH-I and -II neurons in European starling (Sturnus vulgaris) brain. Cloned starling GnIH precursor cDNA encoded three peptides that possess characteristic LPXRF-amide (X = L or Q) motifs at the C termini. Starling GnIH was further identified as SIKPFANLPLRF-NH2 by mass spectrometry combined with immunoaffinity purification. GnIH neurons, identified by in situ hybridization and immunocytochemistry (ICC), were clustered in the hypothalamic paraventricular nucleus. GnIH immunoreactive fiber terminals were present in the external layer of the median eminence in addition to the preoptic area and midbrain, where GnRH-I and GnRH-II neuronal cell bodies exist, respectively. GnIH axon terminals on GnRH-I and -II neurons were shown by GnIH and GnRH double-label ICC. Furthermore, the expression of starling GnIH receptor mRNA was identified in both GnRH-I and GnRH-II neurons by in situ hybridization combined with GnRH ICC. The cellular localization of GnIH receptor has not previously been identified in any vertebrate brain. Thus, GnIH may regulate reproduction of vertebrates by directly modulating GnRH-I and GnRH-II neuronal activity, in addition to influencing the pituitary gland.

scholarly journals Specific detection of neuronal cell bodies: in situ hybridization with a biotin-labeled neurofilament cDNA probe.

1986 ◽  
Vol 34 (7) ◽  
pp. 923-926 ◽  
Author(s):  
P Liesi ◽  
J P Julien ◽  
P Vilja ◽  
F Grosveld ◽  
L Rechardt
Keyword(s):  
In Situ Hybridization ◽  
Cdna Probe ◽  
Neuronal Cell ◽  
Dna Probe ◽  
Specific Detection ◽  
Neurofilament Protein ◽  
Antibody Staining ◽  
Neuronal Cell Bodies ◽  
Specific Mrna

We have used a biotinylated, 300-nucleotide cDNA probe which encodes the 68,000 MW neurofilament protein to detect neurofilament-specific mRNA in situ. The neurofilament message specifically demonstrates the neuronal cell bodies, in contrast to the usual antibody staining which detects their neurites. The hybridization is detected only in neuronal structures. Consequently, detection of the biotinylated neurofilament DNA probe by silver-intensified streptavidin-gold can be specifically used to identify neuronal cell bodies.

Cellular Localization of Inducible Nitric Oxide Synthase in Experimental Endotoxic Shock in the Rat

1994 ◽  
Vol 87 (2) ◽  
pp. 179-186 ◽  
Author(s):  
H. Terence Cook ◽  
Alison J. Bune ◽  
Albertine S. Jansen ◽  
G. Michael Taylor ◽  
Rashpal K. Loi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
In Situ Hybridization ◽  
Cellular Localization ◽  
Endotoxic Shock ◽  
No Synthase ◽  
Similar Distribution ◽  
Mouse Macrophage ◽  
Inducible No Synthase ◽  
Monocytes And Macrophages

1. Endotoxin induces a shock-like syndrome with increased nitric oxide synthesis. To clarify the cellular source of NO in endotoxic shock we used immunohistochemistry and in situ hybridization to localize inducible NO synthase in rats given lipopolysaccharide or Corynebacterium parvum and lipopolysaccharide. Immunohistochemistry was carried out with an antibody raised against a synthetic peptide of mouse macrophage NO synthase. In situ hybridization was performed with 35S-labelled oligonucleotide probes corresponding to cDNA sequences common to mouse macrophage inducible NO synthase and rat vascular smooth inducible NO synthase. Monocytes and macrophages were identified by immunohistochemistry with the mouse monoclonal antibody ED1. 2. After lipopolysaccharide alone, the major site of NO synthase induction was monocytes and macrophages in multiple organs, principally liver and spleen. Bronchial, bile duct, intestinal and bladder epithelium and some hepatocytes also expressed inducible NO synthase. Expression peaked at 5 h and had returned to normal by 12 h except in spleen. 3. After priming with C. parvum, lipopolysaccharide led to a similar distribution of inducible NO synthase as lipopolysaccharide alone, but in addition there was more prominent hepatocyte staining, staining in macrophage granulomas in the liver and inducible NO synthase was present in some endothelial cells in the aorta. 4. These findings provide a direct demonstration of the cellular localization of inducible NO synthase after lipopolysaccharide.

A method for cellular localization of gene expression via quantitative in situ hybridization in plants

2007 ◽  
Vol 50 (1) ◽  
pp. 159-187 ◽  
Author(s):  
Hendrik Küpper ◽  
Laura Ort Seib ◽  
Mayandi Sivaguru ◽  
Owen A. Hoekenga ◽  
Leon V. Kochian
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Cellular Localization

scholarly journals Synthesis of transthyretin (pre-albumin) mRNA in choroid plexus epithelial cells, localized by in situ hybridization in rat brain.

1986 ◽  
Vol 34 (7) ◽  
pp. 949-952 ◽  
Author(s):  
A J Stauder ◽  
P W Dickson ◽  
A R Aldred ◽  
G Schreiber ◽  
F A Mendelsohn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Epithelial Cells ◽  
Rat Brain ◽  
Choroid Plexus ◽  
Cellular Localization ◽  
Lateral Ventricles ◽  
The Brain ◽  
Albumin Mrna ◽  
Choroid Plexus Epithelial

The sites of synthesis of transthyretin in the brain were investigated using in situ hybridization with [35S]-labeled recombinant cDNA probes specific for transthyretin mRNA. Autoradiography of hybridized coronal sections of rat brain revealed specific cellular localization of transthyretin mRNA in choroid plexus epithelial cells of the lateral, third, and fourth ventricles. Transferrin mRNA was also investigated and, in contrast to transthyretin mRNA, was localized mainly in the lateral ventricles. Our results indicate that substantial synthesis of transthyretin and transferrin mRNA may occur in the choroid plexus.

Localization of progesterone-associated endometrial protein mRNA by in-situ hybridization in human pregnancy decidua, endometriosis and borderline endometrioid adenoma

1993 ◽  
Vol 10 (1) ◽  
pp. 71-77 ◽  
Author(s):  
M Kämäräinen ◽  
I Leivo ◽  
M Julkunen ◽  
M Seppälä
Keyword(s):  
In Situ Hybridization ◽  
Cellular Localization ◽  
First Trimester ◽  
Glandular Epithelium ◽  
Hybridization Histochemistry ◽  
Human Pregnancy ◽  
In Situ Hybridization Histochemistry

ABSTRACT Progesterone-associated endometrial protein (PAEP) has been isolated from human decidualized endometrium. In-situ hybridization histochemistry was employed to determine the cellular localization of PAEP mRNA in decidua during pregnancy. PAEP mRNA was found to be expressed in the glandular epithelium of decidua spongiosa throughout pregnancy. Substantial variations in the amount of PAEP mRNA during the course of pregnancy were observed, and it was most abundant at the end of the first trimester. We also found that the PAEP gene was expressed in endometriosis and in a borderline endometrioid adenoma. As in decidual tissues, PAEP mRNA in endometriosis was abundant in the glandular compartment.

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