scholarly journals Zone-Dependent Expression of Estrogen Receptors α and β in Human Benign Prostatic Hyperplasia

2003 ◽  
Vol 88 (3) ◽  
pp. 1333-1340 ◽  
Author(s):  
Toshifumi Tsurusaki ◽  
Daiyu Aoki ◽  
Hiroshi Kanetake ◽  
Satoshi Inoue ◽  
Masami Muramatsu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
In Situ Hybridization ◽  
Benign Prostatic Hyperplasia ◽  
Estrogen Receptors ◽  
Stromal Cells ◽  
Crucial Role ◽  
Peripheral Zone ◽  
Prostatic Hyperplasia ◽  
Cellular Distribution

Estrogen, which acts through estrogen receptors (ERs) α and β, has been implicated in the pathogenesis of benign and malignant human prostatic tumors, i.e. benign prostatic hyperplasia and prostate cancer, thought to originate from different zones of the prostate [the transition zone (TZ) and peripheral zone (PZ), respectively]. Here, we examined the cellular distribution of ERα and ERβ in human normal and hyperplastic prostate tissues, using in situ hybridization and immunohistochemistry. ERα expression was restricted to stromal cells of PZ. In contrast, ERβ was expressed in the stromal cells of PZ as well as TZ. ERβ-positive epithelial cells were evenly distributed in PZ and TZ of the prostate. Our results suggest that estrogen may play a crucial role in the pathogenesis of benign prostatic hyperplasia through ERβ.

Citrate as an in vivo marker to discriminate prostate cancer from benign prostatic hyperplasia and normal prostate peripheral zone: Detection via localized proton spectroscopy

Urology ◽  
1995 ◽  
Vol 45 (3) ◽  
pp. 459-466 ◽  
Author(s):  
John Kurhanewicz ◽  
Daniel B. Vigneron ◽  
Sarah J. Nelson ◽  
Hedvig Hricak ◽  
Jeffrey M. MacDonald ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Peripheral Zone ◽  
Prostatic Hyperplasia ◽  
Proton Spectroscopy ◽  
Normal Prostate

scholarly journals A System for Studying Epithelial-Stromal Interactions Reveals Distinct Inductive Abilities of Stromal Cells from Benign Prostatic Hyperplasia and Prostate Cancer

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Wendy W. Barclay ◽  
Ralph D. Woodruff ◽  
M. Craig Hall ◽  
Scott D. Cramer
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Epithelial Cell ◽  
Efficient Method ◽  
Stromal Cells ◽  
Peripheral Zone ◽  
Renal Tissue ◽  
Prostatic Hyperplasia ◽  
Epithelial Cell Line ◽  
Blue Dye

The development of normal and abnormal glandular structures in the prostate is controlled at the endocrine and paracrine levels by reciprocal interactions between epithelium and stroma. To study these processes, it is useful to have an efficient method of tissue acquisition for reproducible isolation of cells from defined histologies. Here we assessed the utility of a standardized system for acquisition and growth of prostatic cells from different regions of the prostate with different pathologies, and we compared the abilities of stromal cells from normal peripheral zone, benign prostatic hyperplasia (BPH-S), and cancer to induce the growth of a human prostatic epithelial cell line (BPH-1) in vivo. Using the tissue recombination method, we showed that grafting stromal cells (from any histology) alone or BPH-1 epithelial cells alone produced no visible grafts. Recombining stromal cells from normal peripheral zone with BPH-1 cells also produced no visible grafts (n = 15). Recombining BPH-S with BPH-1 cells generated small, well-organized, and sharply demarcated grafts approximately 3–4 mm in diameter (n = 9), demonstrating a moderate inductive ability of BPH-S. Recombining stromal cells from cancer with BPH-1 cells generated highly disorganized grafts that completely surrounded the host kidney and invaded into adjacent renal tissue, demonstrating induction of an aggressive phenotype. We conclude that acquisition of tissue from toluidine blue dye-stained specimens is an efficient method to generate high-quality epithelial and/or stromal cultures. Stromal cells derived by this method from areas of BPH and cancer induce epithelial cell growth in vivo, which mimics the natural history of these diseases.

scholarly journals Molecular Detection of Human Herpes Virus-8 in Prostatic Adenocarcinoma and Benign Prostatic Hyperplasia Tissues by DNA -In Situ Hybridization

2021 ◽  
pp. 96-107
Author(s):  
Zainab Mahdi Saleh ◽  
Jinan Al-Saffar ◽  
Saad Hassan
Keyword(s):  
In Situ Hybridization ◽  
Benign Prostatic Hyperplasia ◽  
Prostatic Hyperplasia ◽  
Prostatic Adenocarcinoma ◽  
Human Herpes ◽  
Human Herpes Virus 8 ◽  
Human Herpes Virus ◽  
Group Detection ◽  
Herpès Virus

Human Herpes Virus-8 (HHV-8) is a sexually transmitted viral infection that can infect the prostate epithelium in immunocompromised adults. Recently, HHV-8 was related to the development and progression of several human malignancies like prostatic adenocarcinoma. This retrospective research was designed to analyze the distribution and possible impact of HHV-8 infection on prostatic adenocarcinogenesis. A total number of one hundred formalin-fixed prostatic tissues were enrolled in this research; forty Prostate Adenocarcinoma (PAC) biopsies, forty biopsies from Benign Prostatic Hyperplasia (BPH), and twenty Apparently Normal Prostatic Tissues (ANPT) as a control group. Detection of HHV -8 DNA was achieved by a highly-sensitive variant of Chromogenic In Situ Hybridization (CISH) technique. In the mean age of PAC patients was 64.08±7.54years. Detection of CISH reactions for HHV 8- DNA was observed in tissues of 70% (28 out of 40) of PAC patients and 30% (12 out of 40) of BPH tissues, whereas no positive reactions were detected in the ANPT group. Detection of CISH reactions for HHV 8- DNA was observed in 55.6%  of tissues of PAC patients  with well grade histopathological examination,  87.5% of moderate grade, and 69.6% of poorly differentiated grade. It can be concluded that HHV-8 infection might contribute in prostate oncogenesis, together with other essential oncogenic viruses.

scholarly journals Chromogenic in Situ Hybridization for Human Cytomegalovirus-DNA Detection in Tissue Subsets with Prostatic Adenocarcinoma and Benign Hyperplasia

2021 ◽  
pp. 2894-2905
Author(s):  
Tayseer Anmar Hassan ◽  
Jenan M. Jawad AL-Saffar ◽  
Saad Hasan Mohammed Ali
Keyword(s):  
In Situ Hybridization ◽  
Benign Prostatic Hyperplasia ◽  
Human Cytomegalovirus ◽  
Dna Detection ◽  
Prostatic Hyperplasia ◽  
Prostatic Adenocarcinoma ◽  
Wide Range ◽  
Chromogenic In Situ Hybridization ◽  
Benign Hyperplasia

     Human cytomegalovirus (HCMV) infects a wide range of human cells, resulting in both benign and malignant tumors. In the last few decades, proteins and/or nucleic acids of the virus were found to be often highly expressed in in patients with basal cell hyperplasia and prostatic neoplasia. This research aimed to unravel the rate of HCMV infections among prostatic tissue subsets from Iraqi patients with adenocarcinoma and benign hyperplasia. One hundred, formalin-fixed and paraffin embedded prostatic tissues were obtained from 40 tissue samples collected from different grades of prostate carcinoma; 40 from benign prostatic hyperplasia and 20 from apparently healthy prostatic tissues. These tissue specimens were collected from the archives of different public and private histopathological laboratories in Baghdad. Detection of HCMV-DNA was achieved by a highly sensitive version of chromogenic in situ hybridization technique. The signals of chromogenic in situ hybridization reactions for HCMV-DNA detection in prostatic adenocarcinoma tissues were found in 65% (26 out of 40) of the tissues, whereas in BPH (Benign Prostatic Hyperplasia), HCMV-DNA was detected in 57.5% (23 out of 40) of the tissues, and in the healthy control group in 25% (5 out of 20) of the tissues. The highest percentage of positive- HCMV- DNA-CISH reactions (57.5%) was found in prostatic adenocarcinomatous tissues that showed poor differentiation. Our results could show that HCMV might contribute to the development of the studied subsets of prostatic adenocarcinoma and benign prostatic hyperplasia.

scholarly journals Benign prostatic hyperplasia (BPH) epithelial cell line BPH-1 induces aromatase expression in prostatic stromal cells via prostaglandin E2

2007 ◽  
Vol 195 (1) ◽  
pp. 89-94 ◽  
Author(s):  
Quan Wu ◽  
Ying Zhou ◽  
Linfeng Chen ◽  
Jiandang Shi ◽  
Chun-Yu Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Epithelial Cell ◽  
Stromal Cells ◽  
Prostate Cancer Cell ◽  
Prostatic Hyperplasia ◽  
Epithelial Cell Line ◽  
Prostate Cancer Cell Lines ◽  
Aromatase Expression ◽  
Cox 2

Estradiol (E2) level in stroma of benign prostatic hyperplasia (BPH) increases with age, and this increase was associated with an elevated expression of aromatase in prostatic stromal cells (PrSCs). Here, we showed that conditioned medium (CM) of BPH-1 (a benign hyperplastic prostatic epithelial cell line), but not of prostate cancer cell lines (LNCaP, DU-145, and PC-3), stimulates aromatase expression in PrSCs. Cyclooxygenase-2 (COX-2) mRNA level in BPH-1, as well as prostaglandin E2 (PGE2) concentration in BPH-1 CM, was significantly higher than that of prostate cancer cell lines. CM of BPH-1 treated with NS-398 (a specific inhibitor of COX-2) failed to stimulate aromatase expression in PrSCs. And PGE2 can stimulate aromatase expression in PrSCs. Our data suggested that BPH-1 induced aromatase expression in PrSCs through the production of PGE2 in a paracrine mechanism.

468: Polymorphic CA Repeats in IGF-I Gene is a Common Genetic Modifier in the Etiology of Prostate Cancer and Benign Prostatic Hyperplasia

2004 ◽  
Vol 171 (4S) ◽  
pp. 125-125
Author(s):  
Lizhong Wang ◽  
Kazunari Sato ◽  
Norihiko Tsuchiya ◽  
Chikara Ohyama ◽  
Shigeru Satoh ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Genetic Modifier ◽  
Prostatic Hyperplasia ◽  
Igf I ◽  
I Gene

scholarly journals On the Origin of Tetraploid Vernal Grasses (Anthoxanthum) in Europe

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 966
Author(s):  
Zuzana Chumová ◽  
Terezie Mandáková ◽  
Pavel Trávníček
Keyword(s):  
Flow Cytometry ◽  
In Situ Hybridization ◽  
Crucial Role ◽  
Evolutionary History ◽  
Essential Role ◽  
Grass Species ◽  
Polyploid Species ◽  
Anthoxanthum Odoratum ◽  
European Populations

Polyploidy has played a crucial role in the evolution of many plant taxa, namely in higher latitudinal zones. Surprisingly, after several decades of an intensive research on polyploids, there are still common polyploid species whose evolutionary history is virtually unknown. Here, we addressed the origin of sweet vernal grass (Anthoxanthum odoratum) using flow cytometry, DNA sequencing, and in situ hybridization-based cytogenetic techniques. An allotetraploid and polytopic origin of the species has been verified. The chromosome study reveals an extensive variation between the European populations. In contrast, an autopolyploid origin of the rarer tetraploid vernal grass species, A. alpinum, has been corroborated. Diploid A. alpinum played an essential role in the polyploidization of both European tetraploids studied.

scholarly journals Light-controlled calcium signalling in prostate cancer and benign prostatic hyperplasia

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Vipin Sharma ◽  
Rita Rana ◽  
Ruma Baksi ◽  
Swapnil P. Borse ◽  
Manish Nivsarkar
Keyword(s):  
Prostate Cancer ◽  
Benign Prostatic Hyperplasia ◽  
Calcium Signalling ◽  
Prostatic Hyperplasia
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