scholarly journals Expression and Cellular Localization of Keratinocyte Growth Factor and Its Receptor in Human Hyperplastic Prostate Tissue1

1998 ◽  
Vol 83 (6) ◽  
pp. 2186-2191
Author(s):  
Alessandra De Bellis ◽  
Clara Crescioli ◽  
Cecilia Grappone ◽  
Stefano Milani ◽  
Paola Ghiandi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cellular Localization ◽  
Keratinocyte Growth Factor

scholarly journals Progesterone-dependent expression of keratinocyte growth factor mRNA in stromal cells of the primate endometrium: keratinocyte growth factor as a progestomedin.

1994 ◽  
Vol 125 (2) ◽  
pp. 393-401 ◽  
Author(s):  
T Koji ◽  
M Chedid ◽  
J S Rubin ◽  
O D Slayden ◽  
K G Csaky ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Growth Factor ◽  
Smooth Muscle Cells ◽  
Stromal Cells ◽  
Cellular Localization ◽  
Keratinocyte Growth Factor ◽  
Muscle Cells ◽  
Northern Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ru 486

In vitro studies have shown that keratinocyte growth factor (KGF, also known as FGF-7) is secreted by fibroblasts and is mitogenic specifically for epithelial cells. Therefore, KGF may be an important paracrine mediator of epithelial cell proliferation in vivo. Because stromal cells are thought to influence glandular proliferation in the primate endometrium, we investigated the hormonal regulation and cellular localization of KGF mRNA expression in the rhesus monkey uterus. Tissues were obtained both from naturally cycling monkeys in the follicular and luteal phases of the cycle, and from spayed monkeys that were either untreated or treated with estradiol (E2) alone, E2 followed by progesterone (P), E2 plus P, or E2 plus P plus an antiprogestin (RU 486). Northern blot analysis of total RNA with 32P-labeled probes revealed that the level of KGF mRNA in the endometrium was 70-100-fold greater in the luteal phase or after P treatment than in untreated, E2-treated, or follicular phase animals. Northern analysis also showed that KGF mRNA was present in the myometrium but was unaffected by hormonal state. RU 486 treatment prevented the P-induced elevation of endometrial KGF mRNA. P-dependent elevation of endometrial KGF expression was confirmed by measurement of KGF protein in tissue extracts using a two-site enzyme-linked immunosorbent assay. In situ hybridization with nonradioactive digoxigenin-labeled cDNA probes revealed that the KGF mRNA signal, which was present only in stromal and smooth muscle cells, was substantially increased by P primarily in the stromal cells located in the basalis region. Smooth muscle cells in the myometrium and the walls of the spiral arteries also expressed KGF mRNA, but the degree of this expression did not differ with hormonal state. P treatment led to increased proliferation in the glandular epithelium of the basalis region and to extensive growth of the spiral arteries. We conclude that the P-dependent increase in endometrial KGF resulted from a dual action of P: (a) a P-dependent induction of KGF expression in stromal cells, especially those in the basalis (zones III and IV), and (b) a P-dependent increase in the number of KGF-positive vascular smooth muscle cells caused by the proliferation of the spiral arteries. KGF is one of the first examples in primates of a P-induced, stromally derived growth factor that might function as a progestomedin.

Keratinocyte growth factor binds to collagens of the gastrointestinal tract: Identification of the collagen binding structure

2001 ◽  
Vol 120 (5) ◽  
pp. A695-A695
Author(s):  
M RUEHL ◽  
I SCHOENFELDER ◽  
R FARNDALE ◽  
G KNIGHT ◽  
R SOMASUNDARAM ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Collagen Binding ◽  
Binding Structure

Keratinocyte Growth Factor (KGF) beeinflusst das Wachstum von Hepatom-Zellen in vitro

2005 ◽  
Vol 43 (05) ◽  
Author(s):  
M Mühlbauer ◽  
T Amann ◽  
J Schölmerich ◽  
A Bosserhoff ◽  
C Hellerbrand
Keyword(s):  
Growth Factor ◽  
Keratinocyte Growth Factor

Molekulare Mechanismen des Schutzes vor einer Naphthalin-induzierten Schädigung des Atemwegepithels der Maus durch Keratinocyte Growth Factor in vivo

Pneumologie ◽  
2007 ◽  
Vol 61 (S 1) ◽  
Author(s):  
AÖ Yildirim ◽  
M Veith ◽  
L Van Winkle ◽  
C Plopper ◽  
H Fehrenbach
Keyword(s):  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Molekulare Mechanismen

Efficacy of keratinocyte growth factor in prevention of oral mucositis in children undergoing allogeneic hematopoietic cell transplantation

2020 ◽  
Vol 51 (3) ◽  
pp. 172-178
Author(s):  
Natalia Bartoszewicz ◽  
Krzysztof Czyżewski ◽  
Robert Dębski ◽  
Anna Krenska ◽  
Ewa Demidowicz ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Transplantation ◽  
Oral Mucositis ◽  
Hematopoietic Cell Transplantation ◽  
Clinical Course ◽  
Keratinocyte Growth Factor ◽  
Supportive Therapy ◽  
Primary Objective ◽  
Hematopoietic Cell ◽  
Unrelated Donor

AbstractIntroductionOral mucositis is regarded by patients as one of the worst and debilitating complications of conditioning and hematopoietic cell transplantation (HCT). Prevention of mucositis is one of the priorities of supportive therapy during and after conditioning.ObjectivesThe primary objective of the study was the analysis of efficacy of keratinocyte growth factor (KGF, palifermin) used in prophylaxis of oral mucositis in patients undergoing allo-HCT. The secondary objectives of the study included the analysis of the influence of palifermin on clinical course of oral mucositis and early transplant outcomes, as well as analysis of the contraindications of palifermin in patients undergoing allo-HCT.Patients and methodsA total number of 253 allo-HCT performed between 2003 and 2018 in patients aged 0–19 years in a single center were analyzed. Overall, in 161 HCTs, palifermin was administered.ResultsPatients receiving KGF were transplanted earlier in the context of calendar year, and more often received ATG, mainly due to the higher rate of unrelated donor transplants. Allo-HCT patients who were administered palifermin had shorter time of mucositis (median: 9 vs. 13 days, p < 0.001), lower mucositis grade (median: 2° vs. 3°; p < 0.001), shorter period of total parenteral nutrition (median: 19 vs. 22 days; p = 0.018), and lower incidence of episodes of febrile neutropenia (median: 39.1% vs. 83.1%; p < 0.001).ConclusionsThe use of palifermin has decreased duration and severity of oral mucositis in children after allo-HCT. Palifermin is a safe and well-tolerated compound in children undergoing allo-HCT.

Controlled release of KGF-2 for regulation of wound healing by KGF-2 complexed with “lotus seedpod surface-like” porous microsphere

2021 ◽  
Author(s):  
Hao Pan ◽  
Changcan Shi ◽  
Rongshuai Yang ◽  
Guanghui Xi ◽  
Chao Lu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Controlled Release ◽  
Growth Factor ◽  
Normal Tissue ◽  
Keratinocyte Growth Factor ◽  
Tissue Structure ◽  
Effective Strategy ◽  
Proliferation And Differentiation ◽  
Porous Microsphere ◽  
Lotus Seedpod

Keratinocyte growth factor-2 (KGF-2) plays a remarkable role in maintaining normal tissue structure and promoting wound healing by regulation the proliferation and differentiation of keratinocyte. As an effective strategy, KGF-2...

Potential aggregation hot spots in recombinant human keratinocyte growth factor: a computational study

2021 ◽  
pp. 1-16
Author(s):  
Mansoureh Shahbazi Dastjerdeh ◽  
Mohammad Ali Shokrgozar ◽  
Hamzeh Rahimi ◽  
Majid Golkar
Keyword(s):  
Growth Factor ◽  
Hot Spots ◽  
Computational Study ◽  
Keratinocyte Growth Factor ◽  
Human Keratinocyte

scholarly journals EphA4 Obstructs Spinal Cord Neuron Regeneration by Promoting Excessive Activation of Astrocytes

2021 ◽  
Author(s):  
Xiaogang Chen ◽  
Lin Zhang ◽  
Fu Hua ◽  
Yu Zhuang ◽  
Huan Liu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Growth Factor ◽  
Neurite Outgrowth ◽  
Neurotrophic Factors ◽  
Cellular Localization ◽  
Axon Regeneration ◽  
Chondroitin Sulphate ◽  
Cortical Cell ◽  
Inflammatory Factors ◽  
Cord Injury

AbstractStudies have found that molecular targets that regulate tissue development are also involved in regulating tissue regeneration. Erythropoietin-producing hepatocyte A4 (EphA4) not only plays a guiding role in neurite outgrowth during the development of the central nervous system (CNS) but also induces injured axon retraction and inhibits axon regeneration after spinal cord injury (SCI). EphA4 targets several ephrin ligands (including ephrin-A and ephrin-B) and is involved in cortical cell migration, axon guidance, synapse formation and astrocyte function. However, how EphA4 affects axon regeneration after SCI remains unclear. This study focuses on the effect and mechanism of EphA4-regulated astrocyte function in neuronal regeneration after SCI. Our research found that EphA4 expression increased significantly after SCI and peaked at 3 days post-injury; accordingly, we identified the cellular localization of EphA4 and ephrin-B ligands in neurons and astrocytes after SCI. EphA4 was mainly expressed on the surface of neurons, ephrin-B1 and ephrin-B3 were mainly localized on astrocytes, and ephrin-B2 was distributed on both neurons and astrocytes. To further elucidate the effect of EphA4 on astrocyte function after SCI, we detected the related cytokines secreted by astrocytes in vivo. We found that the levels of neurotrophic factors including nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) increased significantly after SCI (NGF peaked at 3 days and bFGF peaked at 7 days); the expression of laminin and fibronectin increased gradually after SCI; the expression of inflammatory factors [interleukin (IL)-1β and IL-6] increased significantly from 4 h to 7 days after SCI; and the levels of glial fibrillary acidic protein (GFAP), a marker of astrocyte activation, and chondroitin sulphate proteoglycan (CSPG), the main component of glial scars, both peaked at 7 days after SCI. Using a damaged astrocyte model in vitro, we similarly found that the levels of related cytokines increased after injury. Consequently, we observed the effect of damaged astrocytes on neurite outgrowth and regeneration, and the results showed that damaged astrocytes hindered neurite outgrowth and regeneration; however, the inhibitory effect of injured astrocytes on neurite regeneration was reduced following ephrin-B receptor knockdown or inflammatory inhibition at 24 h after astrocyte injury. Our results showed that EphA4 regulates the secretion of neurotrophic factors, adhesion molecules, inflammatory factors and glial scar formation by binding with the ligand ephrin-B located on the surface of astrocytes. EphA4 affects neurite outgrowth and regeneration after SCI by regulating astrocyte function.

Sign in / Sign up

Export Citation Format

Share Document