scholarly journals Hepatic Insulin Gene Expression as Treatment for Type 1 Diabetes Mellitus in Rats

1997 ◽  
Vol 11 (6) ◽  
pp. 833-837 ◽  
Author(s):  
Patrick Muzzin ◽  
Randy C. Eisensmith ◽  
Kenneth C. Copeland ◽  
Savio L. C. Woo
Keyword(s):  
Gene Expression ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Insulin Gene ◽  
Insulin Gene Expression

scholarly journals Analysis of immune cell components and immune-related gene expression profiles in peripheral blood of patients with type 1 diabetes mellitus

2021 ◽  
Author(s):  
Jian Lin ◽  
Yuanhua Lu ◽  
Bizhou Wang ◽  
Ping Jiao ◽  
Jie Ma
Keyword(s):  
Gene Expression ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Nk Cells ◽  
Immune Cells ◽  
Support Vector ◽  
Hub Genes ◽  
Immune Related Genes

Abstract Background Type 1 diabetes mellitus (T1DM) is a chronic autoimmune disease caused by severe loss of pancreatic β cells. Immune cells are key mediators of β cell destruction. This study attempted to investigate the role of immune cells and immune-related genes in the occurrence and development of T1DM. Methods The raw gene expression profile of the samples from 12 T1DM patients and 10 normal controls was obtained from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified by Limma package in R. The least absolute shrinkage and selection operator (LASSO) - support vector machines (SVM) were used to screen the hub genes. CIBERSORT algorithm was used to identify the different immune cells in distribution between T1DM and normal samples. Correlation of the hub genes and immune cells was analyzed by Spearman, and gene-GO-BP and gene-pathway interaction networks were constructed by Cytoscape plug-in ClueGO. Receiver operating characteristic (ROC) curves were used to assess diagnostic value of genes in T1DM. Results The 50 immune-related DEGs were obtained between the T1DM and normal samples. Then, the 50 immune-related DEGs were further screened to obtain the 5 hub genes. CIBERSORT analysis revealed that the distribution of plasma cells, resting mast cells, resting NK cells and neutrophils had significant difference between T1DM and normal samples. Natural cytotoxicity triggering receptor 3 (NCR3) was significantly related to the activated NK cells, M0 macrophages, monocytes, resting NK cells, and resting memory CD4+ T cells. Moreover, tumor necrosis factor (TNF) was significantly associated with naive B cell and naive CD4+ T cell. NCR3 [Area under curve (AUC) = 0.918] possessed a higher accuracy than TNF (AUC = 0.763) in diagnosis of T1DM. Conclusions The immune-related genes (NCR3 and TNF) and immune cells (NK cells) may play a vital regulatory role in the occurrence and development of T1DM, which possibly provide new ideas and potential targets for the immunotherapy of diabetes mellitus (DM).

Proinsulin 2 Knockout NOD Mice: A Model for Genetic Variation of Insulin Gene Expression in Type 1 Diabetes

Diabetes ◽  
2002 ◽  
Vol 51 (Supplement 3) ◽  
pp. S489-S493 ◽  
Author(s):  
D. Dubois-Lafforgue ◽  
L. Mogenet ◽  
K. Thebault ◽  
J. Jami ◽  
P. Krief ◽  
...  
Keyword(s):  
Gene Expression ◽  
Type 1 Diabetes ◽  
Genetic Variation ◽  
Nod Mice ◽  
Insulin Gene ◽  
Insulin Gene Expression

Reduced CD4+ T-cell-specific gene expression in human type 1 diabetes mellitus

2007 ◽  
Vol 28 (4) ◽  
pp. 177-187 ◽  
Author(s):  
Tihamer Orban ◽  
Janos Kis ◽  
Laszlo Szereday ◽  
Peter Engelmann ◽  
Klara Farkas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
T Cell ◽  
Type 1 Diabetes Mellitus ◽  
Cd4 T Cell ◽  
Specific Gene ◽  
Human Type ◽  
Human Type 1 Diabetes

Type 1 Diabetes and Prediabetic State in a Monozygotic Triplet

1998 ◽  
Vol 47 (3-4) ◽  
pp. 171-176
Author(s):  
M. Krokowski ◽  
M. Abel ◽  
A. Teodorczyk ◽  
A. Szadkowska ◽  
B. Pyrżak ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Genetic Predisposition ◽  
Gene Polymorphisms ◽  
Insulin Gene ◽  
Prediabetic State ◽  
Apo B ◽  
Clinical Onset

AbstractType 1 diabetes mellitus (IDDM) results from a chronic process of autoimmune destruction of ß cells of the Langerhans islets. The presence of autoantibodies (ICA, GADA, anti-IA2, IAA) in serum preceds the clinical onset of the disease. Genetic predisposition for IDDM is connected with HLA, CTLA-4 and insulin gene region.The aim of the study was the genetic and immunological analysis of a triplet. One of them developed Type 1 diabetes mellitus. We analysed HLA class II, CTLA-4 and insulin gene polymorphisms in the whole family. Besides, we investigated immunological status of three brothers.All patients present identical genotype for VNTR loci: D1S80, D17S5 and Apo B, as well as for HLA-DRB1, — DQA1, — DQB1, CTLA-4 gene and all studied insulin gene polymorphisms. That proves their monozigosity. The triplet presents strong genetic predisposition for IDDM. The two patients without overt diabetes have increased levels of ICA, GADA, IA2 and IAA.

Reduced CD4+ T Cell-specific Gene Expression in Human Type 1 Diabetes Mellitus

2007 ◽  
Vol 123 ◽  
pp. S26-S27
Author(s):  
Tihamer Orban ◽  
Janos Kis ◽  
Laszlo Szereday ◽  
Klara Farkas ◽  
Peter Engelmann ◽  
...  
Keyword(s):  
Gene Expression ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
T Cell ◽  
Type 1 Diabetes Mellitus ◽  
Cd4 T Cell ◽  
Specific Gene ◽  
Human Type ◽  
Human Type 1 Diabetes
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