Amounts and modulation of actin mRNAs in mouse oocytes and embryos

Development ◽  
1989 ◽  
Vol 106 (3) ◽  
pp. 561-565 ◽  
Author(s):  
R. Bachvarova ◽  
E.M. Cohen ◽  
V. De Leon ◽  
K. Tokunaga ◽  
S. Sakiyama ◽  
...  
Keyword(s):  
Growth Phase ◽  
Developmental Stages ◽  
Cdna Sequences ◽  
Mouse Oocytes ◽  
Rna Probes ◽  
Sense Strand ◽  
Polysomal Mrna ◽  
Mrna Content ◽  
Actin Mrna ◽  
Specific Sense

In order to measure the content of beta- and gamma-actin mRNA in mouse oocytes and ovulated eggs, Northern and slot blots were hybridized to complementary RNA probes transcribed from mouse isotype-specific cDNA sequences. The blots included samples of isotype-specific sense strand RNA standards prepared from the same cDNA sequences. Total actin mRNA content was estimated to be 40 fg per preovulatory full-grown oocyte or egg, consisting of one-third beta-actin mRNA and two-thirds gamma-actin mRNA. Ninety per cent of the actin mRNA is on polysomes in full-grown oocytes. The per cent of actin mRNA in polysomal mRNA is similar to the per cent of actin in newly synthesized proteins. Measurements on other developmental stages showed that, in mid-growth-phase oocytes, each actin mRNA reaches a level twofold higher than in full-grown oocytes. Thereafter, all modulations of the two isotypic mRNAs occur in parallel; that is, they are maintained at constant levels during the late growth phase (oocytes from females 8–14 days old); gradually degraded in oocytes that have completed their rapid growth phase (oocytes from females 15–18 days old), in maturing oocytes, and in 1- and 2-cell embryos; and deadenylated after about 7 h of progression into meiotic maturation.

scholarly journals Analysis of polysomal mRNA populations of mouse oocytes and zygotes: Dynamic changes in maternal mRNA utilization and function

2006 ◽  
Vol 298 (1) ◽  
pp. 155-166 ◽  
Author(s):  
Santhi Potireddy ◽  
Rita Vassena ◽  
Bela G. Patel ◽  
Keith E. Latham
Keyword(s):  
Dynamic Changes ◽  
Mouse Oocytes ◽  
Maternal Mrna ◽  
Polysomal Mrna ◽  
And Function

scholarly journals Localization of cells producing erythropoietin in murine liver by in situ hybridization [see comments]

Blood ◽  
1991 ◽  
Vol 77 (11) ◽  
pp. 2497-2503 ◽  
Author(s):  
ST Koury ◽  
MC Bondurant ◽  
MJ Koury ◽  
GL Semenza
Keyword(s):  
In Situ Hybridization ◽  
Transgenic Mice ◽  
Antisense Rna ◽  
Interstitial Cells ◽  
Cell Type ◽  
Isolated Cells ◽  
Rna Probes ◽  
Sense Strand ◽  
Murine Liver

Abstract In situ hybridization using antisense RNA probes was used to localize cells that produce erythropoietin (EPO) in the livers of anemic transgenic mice expressing the human EPO gene and in livers of anemic nontransgenic mice. In transgenic mice bled from a hematocrit of 55% to one of 10%, hepatocytes surrounding central veins synthesized large amounts of human EPO mRNA. EPO-producing cells were very rare in the area of portal triads. In transgenic mice bled to a hematocrit of 20%, a similar number and distribution of cells contained human EPO mRNA as was found with a 10% hematocrit, but the cells were less heavily labeled, indicating increased EPO production per cell at 10% hematocrit as compared with 20% hematocrit. No human EPO mRNA was detected in the kidneys of anemic transgenic mice, although endogenous murine EPO mRNA was strongly expressed in cortical interstitial cells. In sections of livers from nontransgenic mice bled from a hematocrit of 45% to one of 10%, only isolated cells produced EPO. When the types of cells could clearly be identified, approximately 80% of these cells were hepatocytes, while 20% had a nonepithelial morphology and were located in or adjacent to the sinusoidal spaces. When the sense strand was used as the RNA probe for in situ hybridization, no labeled cells were seen in normal or anemic livers. These results demonstrate that hepatocytes are responsible for production of EPO in both transgenic and nontransgenic mice and that a second cell type that is similar in morphology to EPO-producing interstitial cells in the kidney also produces EPO in the livers of nontransgenic mice.

Effect of thyroid deficiency on actin mRNA content in the developing rat cerebellum

1990 ◽  
Vol 8 (1) ◽  
pp. 99-106 ◽  
Author(s):  
C. Faivre-Sarrailh ◽  
C. Ferraz ◽  
J. P. Liautard ◽  
A. Rabié
Keyword(s):  
Rat Cerebellum ◽  
Mrna Content ◽  
Actin Mrna ◽  
Developing Rat

Molecular cloning of stage specific mRNAs from amoebae and Plasmodia of Physarum polycephalum

1986 ◽  
Vol 64 (12) ◽  
pp. 1294-1302 ◽  
Author(s):  
Dominick Pallotta ◽  
André Laroche ◽  
Anne Tessier ◽  
Thomas Shinnick ◽  
Gérald Lemieux
Keyword(s):  
Gene Expression ◽  
Molecular Cloning ◽  
Physarum Polycephalum ◽  
Developmental Stages ◽  
The Other ◽  
Cdna Libraries ◽  
Northern Hybridization ◽  
Cdna Sequences ◽  
Specific Mrnas ◽  
Differential Gene

We constructed cDNA libraries from plasmodia and amoebal poly(A)+ RNA of Physarum polycephalum. The libraries were screened by differential hybridization with labeled poly(A)+ RNA of amoebae and plasmodia. The 136 plasmodial specific clones that gave the strongest hybridization signals were analysed in detail. From this group six different cDNA sequences were found. Four of the cDNAs each accounted for between 1 and 4.8% of all the clones in the library and represented abundant mRNAs. Two other clones constituted 0.2 and 0.4% of the total library. Seventeen clones in the amoebal library were amoebal specific. From these clones, seven different sequences were found. One of the sequences was present in nine clones (1.2%) of the library and considered abundant. The other six sequences were each found in only one or two clones. The specificity of these amoebal and plasmodial mRNAs was confirmed by Northern hybridization. Our results show that amoebae and plasmodia have different mRNA populations, which are most likely the result of differential gene expression in these two developmental stages.

scholarly journals Rates of DNA evolution in Drosophila depend on function and developmental stage of expression.

Genetics ◽  
1993 ◽  
Vol 133 (2) ◽  
pp. 291-298
Author(s):  
J R Powell ◽  
A Caccone ◽  
J M Gleason ◽  
L Nigro
Keyword(s):  
Dna Hybridization ◽  
Developmental Stages ◽  
Sequence Data ◽  
Sequence Divergence ◽  
Single Copy ◽  
Evolutionary Divergence ◽  
Cdna Sequences ◽  
Copy Dna ◽  
Dna 2 ◽  
Single Copy Dna

Abstract DNA-sequence divergence of genes expressed in the embryonic stage was compared with the divergence of genes expressed in adults for 13 species of Drosophila representing various degrees of relatedness. DNA-DNA hybridization experiments were conducted using as tracers complementary DNA (cDNA) reversed transcribed from poly(A)+ mRNA isolated from different developmental stages. The results indicate: (1) cDNA is less diverged than total single-copy DNA; (2) cDNA sequences are not in the rapidly evolving fraction of the single-copy genome of Drosophila; (3) early in evolutionary divergence embryonic messages are about half as diverged as adult messages; sequence data from some of the species compared indicate this is likely due to differences in rates of silent substitutions in genes expressed at different stages of development; and (4) at greater evolutionary distance, the differences in embryonic and adult messages disappear; this could be due to lineage-specific shifts in codon usage.

scholarly journals Effects of Osmotic Shrinkage on the Survival of Mouse Oocytes and Embryos at Various Developmental Stages.

1997 ◽  
Vol 14 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Prudencio B. Pedro ◽  
Takashi Sakurai ◽  
Keisuke Edashige ◽  
Magosaburo Kasai
Keyword(s):  
Developmental Stages ◽  
Mouse Oocytes

scholarly journals Knockdown of the Trehalose-6-Phosphate Synthase Gene Using RNA Interference Inhibits Synthesis of Trehalose and Increases Lethality Rate in Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae)

Insects ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 605
Author(s):  
Xinyu Liu ◽  
Zhiwen Zou ◽  
Cong Zhang ◽  
Xian Liu ◽  
Jing Wang ◽  
...  
Keyword(s):  
Growth And Development ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Diaphorina Citri ◽  
Citrus Industry ◽  
Cdna Sequences ◽  
Citrus Huanglongbing ◽  
Citrus Disease ◽  
Trehalose Content ◽  
Phosphate Synthase

Diaphorina citri Kuwayama is the vector of citrus “huanglongbing”, a citrus disease which poses a significant threat to the global citrus industry. Trehalose-6-phosphate synthase (TPS) plays an important role in the regulation of trehalose levels of insects, while its functions in D. citri are unclear. In this study, full-length cDNA sequences of the TPS gene from D. citri (DcTPS) were cloned and its expression patterns at various developmental stages were investigated. The results indicated that DcTPS mRNA was expressed at each developmental stage and the highest DcTPS expression was found in the fifth-instar nymphs of D. citri. Additionally, mortality and deformity of D. citri were observed after 24 and 48 h by feeding with three different dsRNA concentrations (20, 100 and 500 ng/μL). The results indicated that DcTPS expression was declined, and mortality and malformation in nymphs were increased via feeding with dsDcTPS. Moreover, the enzyme and trehalose content were decreased, while the content of glucose was significantly higher than that of untreated (control) individuals. This suggests that DcTPS might be vital for the growth and development of D. citri and further studies of the genes should be related to molting and metabolism for controlling D. citri.

scholarly journals Cloning of abundant mRNA species present during conjugation of Tetrahymena thermophila: identification of mRNA species present exclusively during meiosis.

1983 ◽  
Vol 3 (10) ◽  
pp. 1857-1865 ◽  
Author(s):  
D W Martindale ◽  
P J Bruns
Keyword(s):  
Life Cycle ◽  
Meiotic Prophase ◽  
Tetrahymena Thermophila ◽  
Cross Hybridization ◽  
Colony Hybridization ◽  
Mrna Species ◽  
Cdna Sequences ◽  
Rna Transcripts ◽  
Rna Probes ◽  
Labeled Rna

A cDNA library was constructed by using as a template the RNA present during the meiotic prophase of Tetrahymena thermophila. Clones containing cDNA sequences homologous to moderately abundant to abundant transcripts were detected by colony hybridization and confirmed by hybridizing purified cDNA plasmids on filters with labeled RNA probes. Eighteen clones were isolated, and the sizes of their cDNA inserts were determined. Cross-hybridization of individual cDNA plasmid pairs showed that each of these clones contained cDNA that was homologous to one of eight different RNA transcripts. The sizes of the eight RNA transcripts and the stages of the T. thermophila life cycle during which they were present were determined by hybridizing nick-translated cDNA probes against denatured, electrophoresed RNA from various stages. Clones were identified that contained sequences homologous to RNAs present only in early conjugation (meiosis); other clones contained sequences homologous to RNAs which were abundant during conjugation but present at other stages as well. One clone contained a sequence homologous to an RNA that was abundantly present only in nongrowing cells.

scholarly journals Localization of cells producing erythropoietin in murine liver by in situ hybridization [see comments]

Blood ◽  
1991 ◽  
Vol 77 (11) ◽  
pp. 2497-2503 ◽  
Author(s):  
ST Koury ◽  
MC Bondurant ◽  
MJ Koury ◽  
GL Semenza
Keyword(s):  
In Situ Hybridization ◽  
Transgenic Mice ◽  
Antisense Rna ◽  
Interstitial Cells ◽  
Cell Type ◽  
Isolated Cells ◽  
Rna Probes ◽  
Sense Strand ◽  
Murine Liver

In situ hybridization using antisense RNA probes was used to localize cells that produce erythropoietin (EPO) in the livers of anemic transgenic mice expressing the human EPO gene and in livers of anemic nontransgenic mice. In transgenic mice bled from a hematocrit of 55% to one of 10%, hepatocytes surrounding central veins synthesized large amounts of human EPO mRNA. EPO-producing cells were very rare in the area of portal triads. In transgenic mice bled to a hematocrit of 20%, a similar number and distribution of cells contained human EPO mRNA as was found with a 10% hematocrit, but the cells were less heavily labeled, indicating increased EPO production per cell at 10% hematocrit as compared with 20% hematocrit. No human EPO mRNA was detected in the kidneys of anemic transgenic mice, although endogenous murine EPO mRNA was strongly expressed in cortical interstitial cells. In sections of livers from nontransgenic mice bled from a hematocrit of 45% to one of 10%, only isolated cells produced EPO. When the types of cells could clearly be identified, approximately 80% of these cells were hepatocytes, while 20% had a nonepithelial morphology and were located in or adjacent to the sinusoidal spaces. When the sense strand was used as the RNA probe for in situ hybridization, no labeled cells were seen in normal or anemic livers. These results demonstrate that hepatocytes are responsible for production of EPO in both transgenic and nontransgenic mice and that a second cell type that is similar in morphology to EPO-producing interstitial cells in the kidney also produces EPO in the livers of nontransgenic mice.

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