Epigenetic and genetic factors affect transgene methylation imprinting

Development ◽  
1989 ◽  
Vol 107 (1) ◽  
pp. 165-168 ◽  
Author(s):  
C. Sapienza ◽  
J. Paquette ◽  
T.H. Tran ◽  
A. Peterson
Keyword(s):  
Dna Methylation ◽  
Genetic Factors ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Inbred Mouse Strains ◽  
Maternal Genome ◽  
Two Factors ◽  
In Trans ◽  
In Cis ◽  
Transgene Locus

In some lines of transgenic mice, the methylation of MspI sites within or adjacent to the transgene locus is affected by the sex of the parent from which the transgene is inherited. These differences are consistent with a role for DNA methylation in genome imprinting. In a previous report, we noted that in one such line, all offspring of females exhibited hypermethylation of the transgene while only some offspring of males carried a hypomethylated transgene. In this report, we provide evidence that this phenomenon is controlled by at least two factors, one of which acts in cis and is dependent on the transgene locus, and one of which acts in trans and is supplied by the maternal genome. We also provide evidence that there are genetic differences between inbred mouse strains in the trans-acting factor.

scholarly journals Analysis of Structural Variation Among Inbred Mouse Strains Identifies Genetic Factors for Autism-Related Traits

2021 ◽  
Author(s):  
Ahmed Arslan ◽  
Zhuoqing Fang ◽  
Meiyue Wang ◽  
Zhuanfen Cheng ◽  
Boyoung Yoo ◽  
...  
Keyword(s):  
Genetic Factors ◽  
Sequence Data ◽  
Inbred Mouse ◽  
Inbred Strains ◽  
Autism Spectrum ◽  
Mouse Strains ◽  
Inbred Mouse Strains ◽  
Genetic Traits ◽  
Long Read ◽  
Short Read Sequence

AbstractThe genomes of six inbred strains were analyzed using long read (LR) sequencing. The results revealed that structural variants (SV) were very abundant within the genome of inbred mouse strains (4.8 per gene), which indicates that they could impact genetic traits. Analysis of the relationship between SNP and SV alleles across 53 inbred strains indicated that we have a very limited ability to infer whether SV are present using short read sequence data, even when nearby SNP alleles are known. The benefit of having a more complete map of the pattern of genetic variation was demonstrated by identifying at least three genetic factors that could underlie the unique neuroanatomic and behavioral features of BTBR mice that resemble human Autism Spectrum Disorder (ASD). Similar to the genetic findings in human ASD cohorts, the identified BTBR-unique alleles are very rare, and they cause high impact changes in genes that play a role in neurodevelopment and brain function.

scholarly journals High Throughput Computational Mouse Genetic Analysis

2020 ◽  
Author(s):  
Ahmed Arslan ◽  
Yuan Guan ◽  
Xinyu Chen ◽  
Robin Donaldson ◽  
Wan Zhu ◽  
...  
Keyword(s):  
Genetic Factors ◽  
Inbred Mouse ◽  
Inbred Strains ◽  
Mouse Strains ◽  
Inbred Mouse Strains ◽  
Mitochondrial Targeting ◽  
Phenotypic Data ◽  
Factors Affecting ◽  
Wide Range ◽  
Allelic Differences

AbstractBackgroundGenetic factors affecting multiple biomedical traits in mice have been identified when GWAS data, which measured responses in panels of inbred mouse strains, was analyzed using haplotype-based computational genetic mapping (HBCGM). Although this method was previously used to analyze one dataset at a time; but now, a vast amount of mouse phenotypic data is now publicly available, which could enable many more genetic discoveries.ResultsHBCGM and a whole genome SNP map covering 43 inbred strains was used to analyze 8300 publicly available datasets of biomedical responses (1.52M individual datapoints) measured in panels of inbred mouse strains. As proof of concept, causative genetic factors affecting susceptibility for eye, metabolic and infectious diseases were identified when structured automated methods were used to analyze the output. One analysis identified a novel genetic effector mechanism; allelic differences within the mitochondrial targeting sequence affected the subcellular localization of a protein. We also found allelic differences within the mitochondrial targeting sequences of many murine and human proteins, and these could affect a wide range of biomedical phenotypes.ImplicationsThese initial results indicate that genetic factors affecting biomedical responses could be identified through analysis of very large datasets, and they provide an early indication of how this type of ‘augmented intelligence’ can facilitate genetic discovery.

scholarly journals Profiling DNA methylation differences between inbred mouse strains on the Illumina Human Infinium MethylationEPIC microarray

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0193496 ◽  
Author(s):  
Hemant Gujar ◽  
Jane W. Liang ◽  
Nicholas C. Wong ◽  
Khyobeni Mozhui
Keyword(s):  
Dna Methylation ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Inbred Mouse Strains

scholarly journals THE Ah PHENOTYPE. SURVEY OF FORTY-EIGHT RAT STRAINS AND TWENTY INBRED MOUSE STRAINS

Genetics ◽  
1982 ◽  
Vol 100 (1) ◽  
pp. 79-87
Author(s):  
Daniel W Nebert ◽  
Nancy M Jensen ◽  
Hisashi Shinozuka ◽  
Heinz W Kunz ◽  
Thomas J Gill
Keyword(s):  
Specific Activity ◽  
Inbred Mouse ◽  
Inbred Mouse Strain ◽  
Mouse Strains ◽  
Ah Receptor ◽  
Inbred Mouse Strains ◽  
Sprague Dawley ◽  
Rat Strains ◽  
Specific Activities ◽  
Inbred Rat

ABSTRACT Forty-four inbred and four randombred rat strains and 20 inbred mouse strains were examined for their Ah phenotype by determining the induction of liver microsomal aryl hydrocarbon (benzo[a]pyrene) hydroxylase activity (EC 1.14.14.1) by intraperitoneal treatment with either β-naphthoflavone or 3-methylcholanthrene. All 48 rat strains were found to be Ah-responsive. The maximally induced hydroxylase specific activities of the ALB/Pit, MNR/Pit, MR/Pit, SHR/Pit, and Sprague-Dawley strains were of the same order of magnitude as the basal hydroxylase specific activities of the ACI/Pit, F344/Pit, OKA/Pit, and MNR/N strains. Six of the 20 mouse strains were Ah-nonresponsive (i.e. lacking the normal induction response and presumably lacking detectable amounts of the Ah receptor). The basal hydroxylase specific activities of the BDL/N, NFS/N, STAR/N, and ST/JN mouse strains were more than twice as high as the maximally induced hydroxylase specific activity of the CBA/HT strain.——To date, 24 Ah-nonresponsive mouse strains have been identified, out of a total of 68 known to have been characterized. The reasons for not finding a single Ah-nonresponsive inbred rat strain—as compared with about one Ah-nonresponsive inbred mouse strain found for every three examined—remain unknown.

scholarly journals Relationship between phospholipid transfer protein activity and HDL level and size among inbred mouse strains

1999 ◽  
Vol 40 (2) ◽  
pp. 295-301 ◽  
Author(s):  
John J. Albers ◽  
Wendy Pitman ◽  
Gertrud Wolfbauer ◽  
Marian C. Cheung ◽  
Hal Kennedy ◽  
...  
Keyword(s):  
Inbred Mouse ◽  
Mouse Strains ◽  
Phospholipid Transfer Protein ◽  
Inbred Mouse Strains ◽  
Protein Activity ◽  
Transfer Protein ◽  
Phospholipid Transfer

scholarly journals Heterogeneity of respiratory dendritic cell subsets and lymphocyte populations in inbred mouse strains

2012 ◽  
Vol 13 (1) ◽  
pp. 94 ◽  
Author(s):  
Holger Hackstein ◽  
Andreas Wachtendorf ◽  
Sabine Kranz ◽  
Jürgen Lohmeyer ◽  
Gregor Bein ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Inbred Mouse ◽  
Mouse Strains ◽  
Inbred Mouse Strains ◽  
Dendritic Cell Subsets ◽  
Lymphocyte Populations
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