wingless expression mediates determination of peripheral nervous system elements in late stages of Drosophila wing disc development

We have used conditional wingless genotypes to dissect the role of this gene in late stages of wing disc development. One of these genotypes (wgIL/wg-lacZ) is simultaneously a reporter of wingless transcription and temperature-sensitive for wingless function, and has allowed us to define its pattern of transcription in the absence of wingless activity. The primordia of a subset of the bristles of the notum, which develop in or immediately adjacent to wingless-expressing cells, depend upon wingless activity. The time-course of this contribution and the effect on proneural gene expression together suggest that wingless may regulate the activity of products of the achaete-scute complex in proneural clusters. wingless activity is also required at the presumptive wing margin and is a necessary precondition for the change in proliferation pattern in this region. The involvement of wingless in transducing or mediating positional signals for spatial patterning in imaginal disc development is discussed.

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A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila

eLife ◽

10.7554/elife.22549 ◽

2017 ◽

Vol 6 ◽

Cited By ~ 34

Author(s):

Stefan Harmansa ◽

Ilaria Alborelli ◽

Dimitri Bieli ◽

Emmanuel Caussinus ◽

Markus Affolter

Keyword(s):

Fusion Proteins ◽

Imaginal Disc ◽

Protein Localization ◽

Wing Disc ◽

Drosophila Wing ◽

System A ◽

Polarized Cells ◽

Lateral Plane

The role of protein localization along the apical-basal axis of polarized cells is difficult to investigate in vivo, partially due to lack of suitable tools. Here, we present the GrabFP system, a collection of four nanobody-based GFP-traps that localize to defined positions along the apical-basal axis. We show that the localization preference of the GrabFP traps can impose a novel localization on GFP-tagged target proteins and results in their controlled mislocalization. These new tools were used to mislocalize transmembrane and cytoplasmic GFP fusion proteins in the Drosophila wing disc epithelium and to investigate the effect of protein mislocalization. Furthermore, we used the GrabFP system as a tool to study the extracellular dispersal of the Decapentaplegic (Dpp) protein and show that the Dpp gradient forming in the lateral plane of the Drosophila wing disc epithelium is essential for patterning of the wing imaginal disc.

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Hedgehog activity, independent of decapentaplegic, participates in wing disc patterning

Development ◽

10.1242/dev.124.6.1227 ◽

1997 ◽

Vol 124 (6) ◽

pp. 1227-1237 ◽

Cited By ~ 21

Author(s):

J.L. Mullor ◽

M. Calleja ◽

J. Capdevila ◽

I. Guerrero

Keyword(s):

Zinc Finger ◽

Imaginal Disc ◽

Zinc Finger Protein ◽

Ectopic Expression ◽

Wing Disc ◽

Signal Molecule ◽

Cubitus Interruptus ◽

Drosophila Wing ◽

Finger Protein

In the Drosophila wing imaginal disc, the Hedgehog (Hh) signal molecule induces the expression of decapentaplegic (dpp) in a band of cells abutting the anteroposterior (A/P) compartment border. It has been proposed that Dpp organizes the patterning of the entire wing disc. We have tested this proposal by studying the response to distinct levels of ectopic expression of Hh and Dpp, using the sensory organ precursors (SOPs) of the wing and notum and the presumptive wing veins as positional markers. Here, we show that Dpp specifies the position of most SOPs in the notum and of some of them in the wing. Close to the A/P compartment border, however, SOPs are specified by Hh rather than by Dpp alone. We also show that late signaling by Hh, after setting up dpp expression, is responsible for the formation of vein 3 and the scutellar region, and also for the determination of the distance between veins 3 and 4. One of the genes that mediates the Hh signal is the zinc-finger protein Cubitus interruptus (Ci). These results indicate that Hh has a Dpp-independent morphogenetic effect in the region of the wing disc near the A/P border.

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Regeneration from duplicating fragments of the Drosophila wing disc

Development ◽

10.1242/dev.66.1.117 ◽

1981 ◽

Vol 66 (1) ◽

pp. 117-126

Author(s):

Jane Karlsson ◽

R. J. Smith

Keyword(s):

Imaginal Disc ◽

General Rule ◽

Wing Disc ◽

The Other ◽

Posterior Compartment ◽

Drosophila Wing ◽

Polar Coordinate ◽

New Type ◽

Coordinate Model

It is a general rule that of two complementary Drosophila imaginal disc fragments, one regenerates and the other duplicates. This paper reports an investigation of an exception to this rule. Duplicating fragments from the periphery of the wing disc which lacked presumptive notum were found to regenerate notum structures during and after duplication. The propensity for this was greatest in fragments lying close to the presumptive notum, with the exception of a fragment confined to the posterior compartment, which did not regenerate notum. Structures were added sequentially, and regeneration stopped once most of the notum was present. These results are not easily explained by the polar coordinate model, which states that regeneration cannot occur from duplicating fragments. Since compartments appear to be involved in this type of regeneration as in others, it is suggested that a new type of model is required, one which permits simultaneous regeneration and duplication, and assigns a major role to compartments.

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Role of knot (kn) in Wing Patterning in Drosophila

Genetics ◽

10.1093/genetics/147.3.1203 ◽

1997 ◽

Vol 147 (3) ◽

pp. 1203-1212 ◽

Cited By ~ 1

Author(s):

Katerina Nestoras ◽

Helena Lee ◽

Jym Mohler

Keyword(s):

Hedgehog Signaling ◽

Imaginal Disc ◽

Hedgehog Signaling Pathway ◽

Posterior Compartment ◽

Drosophila Wing ◽

Compartment Boundary ◽

Hh Signaling ◽

Embryonic Segmentation ◽

Anterior Posterior

We have undertaken a genetic analysis of new strong alleles of knot (kn). The original kn1 mutation causes an alteration of wing patterning similar to that associated with mutations of fused (fu), an apparent fusion of veins 3 and 4 in the wing. However, unlike fu, strong kn mutations do not affect embryonic segmentation and indicate that kn is not a component of a general Hh (Hedgehog)-signaling pathway. Instead we find that kn has a specific role in those cells of the wing imaginal disc that are subject to ptc-mediated Hh-signaling. Our results suggest a model for patterning the medial portion of the Drosophila wing, whereby the separation of veins 3 and 4 is maintained by kn activation in the intervening region in response to Hh-signaling across the adjacent anterior-posterior compartment boundary.

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Expression and function of decapentaplegic and thick veins during the differentiation of the veins in the Drosophila wing

Development ◽

10.1242/dev.124.5.1007 ◽

1997 ◽

Vol 124 (5) ◽

pp. 1007-1018 ◽

Cited By ~ 17

Author(s):

J.F. Celis de

Keyword(s):

Imaginal Disc ◽

Signal Transduction Pathways ◽

Pupal Development ◽

Transmembrane Receptors ◽

Notch Ligand ◽

Regulatory Interactions ◽

Drosophila Wing ◽

Signalling Molecule ◽

And Function

The differentiation of the veins in the Drosophila wing involves the coordinate activities of several signal transduction pathways, including those mediated by the transmembrane receptors Torpedo and Notch. In this report, the role of the signalling molecule Decapentaplegic during vein differentiation has been analysed. It is shown that decapentaplegic is expressed in the pupal veins under the control of genes that establish vein territories in the imaginal disc. Decapentaplegic, acting through its receptor Thick veins, activates vein differentiation and restricts expression of both veinlet and the Notch-ligand Delta to the developing veins. Genetic combinations between mutations that increase or reduce Notch, veinlet and decapentaplegic activities suggest that the maintenance of the vein differentiation state during pupal development involves cross-regulatory interactions between these pathways.

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Role of the EGF receptor pathway in growth and patterning of the Drosophila wing through the regulation of vestigial

Development ◽

10.1242/dev.126.5.975 ◽

1999 ◽

Vol 126 (5) ◽

pp. 975-985 ◽

Cited By ~ 6

Author(s):

R. Nagaraj ◽

A.T. Pickup ◽

R. Howes ◽

K. Moses ◽

M. Freeman ◽

...

Keyword(s):

Egf Receptor ◽

Regulatory Gene ◽

Ectopic Expression ◽

Wing Disc ◽

Loss Of Function ◽

Drosophila Wing ◽

Expression Studies ◽

Coordinated Expression ◽

Wing Pouch

Growth and patterning of the Drosophila wing disc depends on the coordinated expression of the key regulatory gene vestigial both in the Dorsal-Ventral (D/V) boundary cells and in the wing pouch. We propose that a short-range signal originating from the core of the D/V boundary cells is responsible for activating EGFR in a zone of organizing cells on the edges of the D/V boundary. Using loss-of-function mutations and ectopic expression studies, we show that EGFR signaling is essential for vestigial transcription in these cells and for making them competent to undergo subsequent vestigial-mediated proliferation within the wing pouch.

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Control of growth and patterning of the Drosophila wing imaginal disc by EGFR-mediated signaling

Development ◽

10.1242/dev.129.6.1369 ◽

2002 ◽

Vol 129 (6) ◽

pp. 1369-1376 ◽

Cited By ~ 2

Author(s):

Myriam Zecca ◽

Gary Struhl

Keyword(s):

Gene Expression ◽

Imaginal Disc ◽

Ectopic Expression ◽

Growth Factor Receptor ◽

Wing Disc ◽

Wing Imaginal Disc ◽

Egfr Signaling ◽

Drosophila Wing ◽

Compartment Boundary ◽

Egfr Ligand

The subdivision of the Drosophila wing imaginal disc into dorsoventral (DV) compartments and limb-body wall (wing-notum) primordia depends on Epidermal Growth Factor Receptor (EGFR) signaling, which heritably activates apterous (ap) in D compartment cells and maintains Iroquois Complex (Iro-C) gene expression in prospective notum cells. We examine the source, identity and mode of action of the EGFR ligand(s) that specify these subdivisions. Of the three known ligands for the Drosophila EGFR, only Vein (Vn), but not Spitz or Gurken, is required for wing disc development. We show that Vn activity is required specifically in the dorsoproximal region of the wing disc for ap and Iro-C gene expression. However, ectopic expression of Vn in other locations does not reorganize ap or Iro-C gene expression. Hence, Vn appears to play a permissive rather than an instructive role in organizing the DV and wing-notum segregations, implying the existance of other localized factors that control where Vn-EGFR signaling is effective. After ap is heritably activated, the level of EGFR activity declines in D compartment cells as they proliferate and move ventrally, away from the source of the instructive ligand. We present evidence that this reduction is necessary for D and V compartment cells to interact along the compartment boundary to induce signals, like Wingless (Wg), which organize the subsequent growth and differentiation of the wing primordium.

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Boundary Dpp promotes growth of medial and lateral regions of the Drosophila wing

eLife ◽

10.7554/elife.22013 ◽

2017 ◽

Vol 6 ◽

Cited By ~ 14

Author(s):

Lara Barrio ◽

Marco Milán

Keyword(s):

Temperature Sensitive ◽

Drosophila Wing ◽

Compartment Boundary ◽

Absolute Requirement ◽

Proliferative Growth ◽

Graded Activity ◽

Tissue Size ◽

Wing Growth ◽

Intense Debate

The gradient of Decapentaplegic (Dpp) in the Drosophila wing has served as a paradigm to characterize the role of morphogens in regulating patterning. However, the role of this gradient in regulating tissue size is a topic of intense debate as proliferative growth is homogenous. Here, we combined the Gal4/UAS system and a temperature-sensitive Gal80 molecule to induce RNAi-mediated depletion of dpp and characterise the spatial and temporal requirement of Dpp in promoting growth. We show that Dpp emanating from the AP compartment boundary is required throughout development to promote growth by regulating cell proliferation and tissue size. Dpp regulates growth and proliferation rates equally in central and lateral regions of the developing wing appendage and reduced levels of Dpp affects similarly the width and length of the resulting wing. We also present evidence supporting the proposal that graded activity of Dpp is not an absolute requirement for wing growth.

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