Nuclear division in the marine dinoflagellate Oxyrrhis marina

1986 ◽  
Vol 85 (1) ◽  
pp. 161-175
Author(s):  
X.P. Gao ◽  
J.Y. Li
Keyword(s):  
Nuclear Envelope ◽  
Phylogenetic Relationships ◽  
Nuclear Division ◽  
Metaphase Plate ◽  
Active Role ◽  
Ultrastructural Changes ◽  
Interphase Nuclei ◽  
Oxyrrhis Marina ◽  
Two Stages ◽  
Anaphase B

The nuclear division of Oxyrrhis marina is a very distinct one among the mitoses of dinoflagellates that have been studies. Using synchronized populations, we have investigated the ultrastructural changes in this nuclear division. In interphase, nuclei can be classified into two groups on the basis of the shapes of the chromosomes. Y- and U-shaped chromosomes have been observed in both types of interphase nuclei. By prophase the nucleus becomes oval, many nuclear plaques appear on the nuclear envelope, and many microtubules radiate from these nuclear plaques within the nucleus. Metaphase can be identified by the characteristic arrangement of the chromosomes; an equatorial metaphase plate is absent. As in many higher organisms, anaphase includes two stages: anaphase A and anaphase B. During anaphase A the nucleus does not apparently elongate and the chromosomes migrate towards the poles by a combination of the shortening of the chromosome-associated microtubules and the elongation of those located between daughter chromosomes. During anaphase B the nucleus elongates to about twice its former length. This elongation may result from growth of the interzonal nuclear envelope. Dividing nucleoli are associated with microtubules, which suggests that microtubules may play an active role in the division of the nucleolus. The evolution of mitosis and the phylogenetic relationships between Oxyrrhis, typical dinoflagellates and Syndinium are discussed.

Ultrastructural changes in nuclear membranes and organelle associations during mitosis of the aquatic fungus Entophlyctis sp.

1975 ◽  
Vol 53 (7) ◽  
pp. 627-646 ◽  
Author(s):  
Martha J. Powell
Keyword(s):  
Endoplasmic Reticulum ◽  
Nuclear Envelope ◽  
Nuclear Division ◽  
Ultrastructural Changes ◽  
Aquatic Fungus ◽  
Electron Microscopic ◽  
Mitotic Apparatus ◽  
Inner Membrane ◽  
Nuclear Membranes ◽  
Spindle Microtubules

Electron microscopic observations on an endobiotic chytrid, Entophlyctis sp., have revealed a mitotic apparatus which is presently unique among fungi. Daughter nuclear envelopes are reconstituted from cisternae apparently proliferated by the inner membrane of the nuclear envelope. Before nuclear division, centrioles replicate and migrate to the poles of the nucleus. Large pores appear at this time in a depression of the nuclear envelope opposite the paired centrioles. This region of the envelope fragments and leaves polar fenestrae as spindle microtubules appear in the nucleus. The inner membrane of the nuclear envelope then invaginates and proliferates cisternae until a layer of inner membrane cisternae lines the original nuclear envelope at late metaphase. Connections between the inner membrane of the original nuclear envelope and the cisternae persist until telophase. As the spindle elongates and the inner membrane cisternae fuse centripetally to form a reticulum around the chromatin mass, the original nuclear envelope opens more at the poles. The reticulum becomes the nuclear envelope of the new daughter nuclei. When the original envelope finally disperses, it is distinguishable from the endoplasmic reticulum only by the presence of pores. Microbodies are consistently associated with the original nuclear envelope and appear adjacent to the new daughter envelopes at the end of telophase. Densely staining arms project from the sides of the primary centrioles toward the polar mitochondria.

scholarly journals The ultrastructure and timing of events in the nuclear cycle of the fungus Entomophaga aulicae

1988 ◽  
Vol 90 (3) ◽  
pp. 501-516
Author(s):  
FAYE MURRIN ◽  
WILLIAM NEWCOMB ◽  
I. BRENT HEATH
Keyword(s):  
Nuclear Envelope ◽  
Linear Array ◽  
Nuclear Division ◽  
Metaphase Plate ◽  
Serial Sections ◽  
Free Zone ◽  
Nuclear Cycle ◽  
Full Complement ◽  
Spindle Microtubules ◽  
Spindle Elongation

The ultrastructure of the mitotic nuclear division cycle of the fungus Entomophaga aulicae was studied from serial sections of hyphal tips and protoplasts. The extranuclear bar-shaped nucleus- associated organelle (NAO) remained associated with the persistent nuclear envelope throughout. Prior to spindle formation, a patch of intranuclear NAO-associated chromatin detached from the nuclear envelope to yield a chromatin free zone containing fine filaments and a linear array of presumptive kinetochores. Early metaphase spindles less than 1μm in length were characterized by a ‘fused’ metaphase plate consisting of kinetochore-associated chromatin and a full complement of at least 15 kinetochore microtubules per half-spindle, while most of the chromatin was remote from the intranuclear spindle. Analysis of the distribution of antiparallel spindle microtubules indicated that polar separation and concomitant spindle elongation through metaphase were not accompanied by intermicrotubule sliding. Anaphase exhibited extensive decondensation of the large patches of condensed chromatin characteristic of all other stages. In a logarithmically growing protoplast population all nuclei contained spindle microtubules, with metaphase occupying approximately 66% of the nuclear cycle time. The calculated genome size of 4.3 pg, and average DNA content per chromosome of 0.3 pg, are extremely high for fungi.

scholarly journals Cell Division in Oedogonium II. Nuclear Division in O. Cardiacum

1970 ◽  
Vol 23 (1) ◽  
pp. 71 ◽  
Author(s):  
JD Pickett-Heaps ◽  
LC Fowke
Keyword(s):  
Cell Division ◽  
Granular Material ◽  
Nuclear Envelope ◽  
Nuclear Division ◽  
Complex Structure ◽  
Metaphase Plate ◽  
Spindle Axis ◽  
Large Numbers

Nuclear division in O. cardiacum is described. Before division, the nucleus enlarges considerably. At prophase, the nucleolus starts dispersing and kinetochores appear on the condensing chromatin, situated and oriented apparently at random in the nucleus. By prometaphase, the kinetochore pairs become aligned along the spindle axis before moving into the metaphase-plate configuration; this supports an earlier theory explaining metakinesis. During prophase and metaphase particularly, the nuclear envelope at the poles forms channels that extend for some distance into the cytoplasm; these may also bifurcate. The nucleolus disperses but remains in the intranuclear spindle throughout division as a loosely knit skein of granular material. The kinetochores have a complex structure, up to seven distinct layers being detectable; the kinetochore pairs split, and then migrate polewards at anaphase with the rest of the chromosome trailing behind. Large numbers of microtubules run from the kinetochore into evaginations of the nuclear envelope which increase in size during anaphase. The spindle grows in length considerably during anaphase, this coinciding with a proliferation of interzonal microtubules, first seen amongst the trailing chromosome arms. The nuclear envelope enclosing the spindle becomes severely stretched at this stage; it contracts closely around each of the daughter nuclei, isolating them from the rest of the spindle (including microtubules and the remains of the nucleolus). The spindle then collapses; the nuclei come together and then flatten against one another; between them, vesicles and other components of the septum collect amongst a large number of transversely oriented micro tubules.

Ultrastructure of Mitosis in the Aquatic Phycomycete Chytriomyces Hyalinus

1980 ◽  
Vol 38 ◽  
pp. 498-499
Author(s):  
David G. Pechak
Keyword(s):  
Life Cycle ◽  
Nuclear Envelope ◽  
Nuclear Division ◽  
Condensed Chromatin ◽  
Nuclear Pores ◽  
Interphase Nuclei ◽  
Aquatic Phycomycete ◽  
Simple Life

The aquatic Phycomycetes, particularly the Chytridiales, are a group of fungi with a rather simple life cycle and morphology. With the advent of ultrastructural investigations on these organisms, two stable characteristics (zoospore ultrastructure and nuclear division) have proven helpful in elucidating relationships in this perplexing taxon. The latter is described in this communication. Conventional techniques were employed in the preparation of the material. Interphase nuclei of C. hyalinus are essentially spherical in shape, measure approximately 2.8 μm in diameter, and have a single, centrally located, nucleus. Prophase nuclei, while not exhibiting condensed chromatin, are characterized by the presence of migrating centrioles and a fusiform or spindle-shape to the nuclear profile (Fig. 1). Nuclear pores predominate near the polar ends of the nuclear envelope. No evidence has been found to indicate that centrioles replicate during prophase.

Ultrastructure of the Fungal Nucleus

1971 ◽  
Vol 9 (2) ◽  
pp. 453-473
Author(s):  
M. GIRBARDT
Keyword(s):  
Nuclear Envelope ◽  
Direct Contact ◽  
Nuclear Division ◽  
Higher Plants ◽  
Compound Structure ◽  
Interphase Nuclei ◽  
Electron Dense Material ◽  
Nuclear Age ◽  
Division Process ◽  
Polar Organelle

The electron-dense material which regularly occurs at the poles of the nuclei of certain fungi during division has been studied in hyphal interphase nuclei of 4 basidio- and 3 ascomycetes. The shape of this material varies with the species and the nuclear age and may be that of a sphere or a dumbbell. In some instances paired diskoidal structures are found in the place of the globular elements of the dumbbell configuration. The organelle in question lies in an invagination of the nuclear envelope preferentially at the forward pointing pole of the nucleus. Its size (0.1-0.5 µm) is correlated with the size of the nucleus. In Polystictus versicolor the diameter of both globular elements of the polar organelle increases during initiation of the division process. The structure is identical with the fungal ‘centriole’ of light-microscopical studies but lacks the characteristic organization of a true centriole. It is suggested that the polar organelle is the equivalent of a kinetochore because it maintains continuous and direct contact with the nuclear envelope (on its cytoplasmic aspect), seems to be connected with the karyoplasm, exhibits oscillating movements, is continuous with microtubules during division and resembles in its fine structure the kinetochores of the chromosomes of higher plants and animals. In addition to its function as an organelle of nuclear motility it also seems to play a part in the initiation of DNA synthesis and nuclear division. If this is correct one would expect to find the genome of the fungal nucleus having the form of a unitary, coherent compound structure (chain) extending from or incorporating a single, solitary kinetochore.

Studies on nuclear division in basidia of Poria latemarginata

1974 ◽  
Vol 52 (11) ◽  
pp. 2323-2333 ◽  
Author(s):  
E. C. Setliff ◽  
H. C. Hoch ◽  
R. F. Patton
Keyword(s):  
Electron Microscopy ◽  
Nuclear Envelope ◽  
Nuclear Division ◽  
Light And Electron Microscopy ◽  
Metaphase Plate ◽  
Longitudinal Axis ◽  
Spindle Pole ◽  
Prophase Nucleus ◽  
Oriented Parallel ◽  
Two Envelopes

Nuclear division in basidia of Poria latemarginata was studied comparatively by light and electron microscopy. Premeiotic mitosis occurred in the lower half of the basidium and was oriented parallel to the longitudinal axis of the basidium. Mitosis was not observed with the light microscope and only late anaphase figures of mitosis were seen with the electron microscope. Mitosis was intranuclear with microtubules oriented between two spindle pole bodies (SPBs). The SPBs were spherical with a central core of material slightly more electron opaque than the surrounding SPB material. The nuclear envelope remained intact except at the SPBs.Divisions I and II of meiosis were chiastobasidial and occurred at the apices of basidia. The major features of meiosis observed by both light and electron microscopy were (1) karyogamy followed by the presence of one or two nucleoli in the prophase nucleus; (2) elongated chromosomes and synapsis at late zygotene – pachytene; (3) occurrence of a spindle at metaphase–anaphase composed of chromosomal and continuous microtubules associated with the SPBs; (4) absence of a metaphase plate with chromosomes arranged randomly around a zone of continuous microtubules; (5) condensation of chromosomes and asynchronous separation at anaphase; (6) kinetochores at anaphase; (7) the nuclear envelope remaining intact throughout meiosis except for discontinuities at the SPBs; (8) membrane-bound vesicles associated with chromosomes during division; and (9) separation of daughter nuclei at telophase. Stages of division II meiosis were observed less frequently and were similar to division I. The four postmeiotic nuclei then migrated back toward the central part of the basidium. Sterigmata developed at this time. Postmeiotic nuclei were surrounded by one or two envelopes of perinuclear endoplasmic reticulum before their migration into basidiospores. Electron-opaque inclusions occurred within the nuclei at this stage.

LUVE: A Constructive Learning Approach for Working with Parents

2002 ◽  
Vol 83 (3) ◽  
pp. 275-284 ◽  
Author(s):  
Lucía Santelices Cuevas ◽  
Verónica Astroza
Keyword(s):  
Sex Education ◽  
Teaching Strategy ◽  
Active Role ◽  
Descriptive Study ◽  
Learning Approach ◽  
Cross Sectional ◽  
Constructive Learning ◽  
Second Stage ◽  
Teachers And Parents ◽  
Two Stages

This study examines the application of a didactic teaching strategy called LUVE. Based on a constructivist learning approach, LUVE's purpose is to develop collaborative links between schools and parents, so that parents can improve their daily educational practices with their children and assume a more active role in their children's education. The project took place in Santiago, Chile, and was implemented in two stages. The first stage included a cross-sectional descriptive study with an experimental design, focusing on parents. The second stage involved teachers and parents who applied and carried out the LUVE strategy, The project's content focused on sex education, as this was the topic of interest to parents. The findings indicate that the strategy was a successful experience for the parents.

Fine structure of the haplosporidan Kernstab, a persistent, intranuclear mitotic apparatus

1975 ◽  
Vol 18 (2) ◽  
pp. 327-346
Author(s):  
F.O. Perkins
Keyword(s):  
Fine Structure ◽  
Nuclear Envelope ◽  
Light Microscope ◽  
Spindle Pole Body ◽  
Spindle Pole ◽  
Interphase Nuclei ◽  
Mitotic Apparatus ◽  
Pole Body

The fine structure of the haplosporidan mitotic apparatus is described from observations of plasmodial nuclei of Minchinia nelsoni, M. costalis, Minchinia sp., and Urosporidium crescens. The apparatus, which is the Kernstab of light-microscope studies, consists of a bundle of microtubules terminating in a spindle pole body (SPB) at each end of the bundle. A few microtubules extend from SPB to SPB, but most either extend from an SPB and terminate in the nucleoplasm or lie in the nucleoplasm, free of either SPB. The bundle lengthens during mitosis, increasing the SPB-to-SPB distance by a factor of 2 to 3 as compared to interphase nuclei. SPBs are not in contact with the nuclear envelope, being found always in the nucleoplasm which is delimited by the nuclear envelope throughout mitosis. The mitotic apparatus is persistent through interphase, at least in a form which is not significantly different from that found in mitotic nuclei.

Maize meiotic spindles assemble around chromatin and do not require paired chromosomes

1998 ◽  
Vol 111 (23) ◽  
pp. 3507-3515 ◽  
Author(s):  
A. Chan ◽  
W.Z. Cande
Keyword(s):  
Nuclear Envelope ◽  
Higher Plants ◽  
Metaphase Plate ◽  
Meiotic Spindle ◽  
Wild Type ◽  
Homologous Chromosomes ◽  
Nuclear Envelope Breakdown ◽  
Meiotic Spindles ◽  
Meiotic Mutations ◽  
Bipolar Spindles

To understand how the meiotic spindle is formed and maintained in higher plants, we studied the organization of microtubule arrays in wild-type maize meiocytes and three maize meiotic mutants, desynaptic1 (dsy1), desynaptic2 (dsy2), and absence of first division (afd). All three meiotic mutations have abnormal chromosome pairing and produce univalents by diakinesis. Using these three mutants, we investigated how the absence of paired homologous chromosomes affects the assembly and maintenance of the meiotic spindle. Before nuclear envelope breakdown, in wild-type meiocytes, there were no bipolar microtubule arrays. Instead, these structures formed after nuclear envelope breakdown and were associated with the chromosomes. The presence of univalent chromosomes in dsy1, dsy2, and afd meiocytes and of unpaired sister chromatids in the afd meiocytes did not affect the formation of bipolar spindles. However, alignment of chromosomes on the metaphase plate and subsequent anaphase chromosome segregation were perturbed. We propose a model for spindle formation in maize meiocytes in which microtubules initially appear around the chromosomes during prometaphase and then the microtubules self-organize. However, this process does not require paired kinetochores to establish spindle bipolarity.

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