The Effect of Certain Fixatives on Particles of a Globular Protein: An Electron-Microscopical Study

1964 ◽  
Vol s3-105 (70) ◽  
pp. 227-230
Author(s):  
K. DEUTSCH ◽  
E. FISCHER ◽  
W. KRAUSE
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Potassium Permanganate ◽  
Bovine Serum ◽  
Osmium Tetroxide ◽  
Microscopical Study ◽  
Globular Protein ◽  
Electron Microscopical Study ◽  
Electron Microscopical

Particles (regarded as molecules) of bovine serum albumin have been studied by electron microscopy both in the untreated state and after fixation with osmium tetroxide, formaldehyde, and potassium permanganate. All three fixatives, especially potassium permanganate, increased the density of the particles considerably.

An Electron Microscopical Study of Isolated Mitochondrial Membranes Treated with Osmium Tetroxide, Potassium Permanganate, and Formaldehyde

1964 ◽  
Vol s3-105 (71) ◽  
pp. 319-323
Author(s):  
K. DEUTSCH ◽  
W. KRAUSE ◽  
S. ROSENTHAL
Keyword(s):  
Potassium Permanganate ◽  
Electron Micrographs ◽  
Osmium Tetroxide ◽  
Microscopical Study ◽  
Elementary Particles ◽  
Electron Microscopical Study ◽  
Mitochondrial Membranes ◽  
Small Particles ◽  
Isolated Mitochondria ◽  
Electron Microscopical

An investigation of isolated mitochondrial membranes, fixed with osmium tetroxide, potassium permanganate, or formaldehyde, shows that a great number of small particles are attached to their surfaces. Sections of specimens fixed in potassium permanganate or osmium tetroxide appear to confirm these findings. An attempt has been made to compare these particles with the ‘elementary particles’ described by Fernández-Morán and by Green, and also with particles revealed by negative staining of mitochondrial membranes, as reported by Stoeckenius. Specimens fixed with formaldehyde, whether isolated mitochondria or material fixed in bulk, show very poor contrast in electron micrographs.

scholarly journals The Glass Transition Behavior of the Globular Protein Bovine Serum Albumin

2003 ◽  
Vol 85 (6) ◽  
pp. 3943-3950 ◽  
Author(s):  
Geoffrey J. Brownsey ◽  
Timothy R. Noel ◽  
Roger Parker ◽  
Stephen G. Ring
Keyword(s):  
Glass Transition ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Globular Protein ◽  
Transition Behavior ◽  
Protein Bovine Serum Albumin

Surface Morphology Investigation of Poly(ether ether ketone) Immobilized with Heparin and Bovine Serum Albumin

2013 ◽  
Vol 683 ◽  
pp. 409-412
Author(s):  
Hui Sun ◽  
Rui Chao Chen ◽  
Biao Yang ◽  
Guo Zhi Xu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Surface Morphology ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Ether Ether Ketone ◽  
Poly Ether Ether Ketone ◽  
Ether Ketone ◽  
Scanning Electron

The surface topography of poly(ether ether ketone) (PEEK) film immobilized with heparin and Bovine Serum Albumin (BSA) was characterized via scanning electron microscopy (SEM). Compared with the unmodified film, the surface of modified film changed and become rough, which indirectly proved the successful introduction of monomer and biomolecule on PEEK

Functionalized diphenylchlorins and bacteriochlorins: their synthesis and bioconjugation for targeted photodynamic therapy and tumour cell imaging

2000 ◽  
Vol 04 (07) ◽  
pp. 655-658 ◽  
Author(s):  
JONATHAN M. SUTTON ◽  
NELSON FERNANDEZ ◽  
ROSS W. BOYLE
Keyword(s):  
Photodynamic Therapy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Tumour Cell ◽  
Bovine Serum ◽  
Osmium Tetroxide ◽  
Cell Imaging ◽  
Important Group ◽  
Structure Activity ◽  
Targeted Photodynamic Therapy

A range of 5,15-diphenylporphyrins with symmetric and unsymmetric substitution patterns were subjected to osmium tetroxide-mediated dihydroxylations. The resulting chlorins and bacteriochlorins present an important group of compounds for studying structure-activity relationships of photodynamic sensitizers. The regioselectivity of the dihydroxylation of various 5,15-diphenylporphyrins with unsymmetrical substitution patterns was also examined. Both diphenylchlorin diol (DPC) and diphenylbacteriochlorin tetrol (DPBC) examples were converted into reactive isothiocyanates and conjugated with bovine serum albumin (BSA).

scholarly journals Design and development of paclitaxel-loaded bovine serum albumin nanoparticles for brain targeting

2011 ◽  
Vol 61 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Amit Bansal ◽  
Deepak Kapoor ◽  
Rishi Kapil ◽  
Neha Chhabra ◽  
Sanju Dhawan
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Correlation Spectroscopy ◽  
Brain Targeting ◽  
Release Mechanism ◽  
Design And Development ◽  
Albumin Nanoparticles

Design and development of paclitaxel-loaded bovine serum albumin nanoparticles for brain targeting Bovine serum albumin (BSA) nanoparticles loaded with paclitaxel (PTX) were prepared using a desolvation technique. A 32 full factorial design (FFD) was employed to formulate nanoparticles. Nanoparticles were characterized for particle size by photon correlation spectroscopy and surface morphology by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Encapsulation efficiency, zeta potential and particle yield were also determined. Response surface linear modelling (RSLM) was used to predict the optimal formulation. Various models were applied to determine the release mechanism from PTX nanoparticles. The effect of drug-polymer ratio on the release profile of formulations was observed and was applied to determine the suitability of the predicted optimal formulation. A preliminary study to determine the feasibility of targeting the prepared nanoparticles to brain was also carried out using mice as in vivo models.

scholarly journals ELECTRON MICROSCOPICAL STUDY OF SKELETAL MUSCLE DURING ISOTONIC (AFTERLOAD) AND ISOMETRIC CONTRACTION

1956 ◽  
Vol 2 (2) ◽  
pp. 201-211 ◽  
Author(s):  
G. G. Knappeis ◽  
F. Carlsen
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Isometric Contraction ◽  
Sarcomere Length ◽  
Microscopical Study ◽  
Electron Microscopical Study ◽  
Semitendinosus Muscle ◽  
Electron Microscopical ◽  
Contraction Band

Bundles of the curarized semitendinosus muscle of the frog were fixed during isotonic (afterload) and isometric contraction and the length of the A and I bands investigated by electron microscopy. The sarcomere length, during afterload contraction initiated at 25 per cent stretch, varied depending on the afterload applied between 3.0 and 1.2 µ, i.e. the shortening amounted to 5 to 50 per cent. The shortening involved both the A and I bands. Between a sarcomere length of 3.0 to 1.7 µ (shortening 5 to 35 per cent) the A bands remained practically constant at about 1.5 µ (6 to 8 per cent shortening); the length of the I bands decreased from 1.4 to 0.3 µ (80 per cent shortening). Below a sarcomere length of 1.7 to 1.2 µ the A bands shortened from 1.5 to 1.0 µ (from 6 to 8 to 25 per cent). At sarcomere lengths 1.6 to 1.2 µ the I band was replaced by a contraction band. During isometric contraction the A bands shortened by about 8 to 10 per cent; the I bands were correspondingly elongated.

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