scholarly journals Adenosine A2A Receptor Agonist PSB-0777 Modulates Synaptic Proteins and AMPA Receptor Expression in a Dose- and Time-Dependent Manner in Rat Primary Cortical Neurons

2020 ◽  
Vol 43 (8) ◽  
pp. 1159-1171
Author(s):  
Shaolei Luo ◽  
Yangyang Hou ◽  
Yaping Zhang ◽  
Tengfei Ma ◽  
Wenping Shao ◽  
...  
Keyword(s):  
Ampa Receptor ◽  
Cortical Neurons ◽  
Receptor Agonist ◽  
Receptor Expression ◽  
Synaptic Proteins ◽  
Time Dependent ◽  
Dependent Manner ◽  
Primary Cortical Neurons

Genetically Controlled Upregulation of Adenosine A1 Receptor Expression Enhances the Survival of Primary Cortical Neurons

2012 ◽  
Vol 46 (2) ◽  
pp. 535-544 ◽  
Author(s):  
Tsvetan Serchov ◽  
Hasan-Cem Atas ◽  
Claus Normann ◽  
Dietrich van Calker ◽  
Knut Biber
Keyword(s):  
Cortical Neurons ◽  
Receptor Expression ◽  
Adenosine A1 Receptor ◽  
Primary Cortical Neurons ◽  
Adenosine A1 ◽  
A1 Receptor

scholarly journals Effects of sodium arsenite on neurite outgrowth and glutamate AMPA receptor expression in mouse cortical neurons

2013 ◽  
Vol 37 ◽  
pp. 197-206 ◽  
Author(s):  
Fumihiko Maekawa ◽  
Takashi Tsuboi ◽  
Manami Oya ◽  
Kyaw Htet Aung ◽  
Shinji Tsukahara ◽  
...  
Keyword(s):  
Neurite Outgrowth ◽  
Ampa Receptor ◽  
Cortical Neurons ◽  
Sodium Arsenite ◽  
Receptor Expression

Regional time-dependent changes in vasopressin V2 receptor expression in the rat kidney during water restriction

1998 ◽  
Vol 274 (5) ◽  
pp. F906-F913 ◽  
Author(s):  
Frank Park ◽  
George Koike ◽  
Allen W. Cowley
Keyword(s):  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Collecting Duct ◽  
Rat Kidney ◽  
Receptor Expression ◽  
Time Dependent ◽  
Dependent Manner ◽  
Water Restriction ◽  
Rt Pcr

Elevations of arginine vasopressin (AVP) binding to renal vasopressin V2 receptors (V2R) enhance water and urea reabsorption in the collecting duct epithelium. This study was designed to quantify the levels of V2R mRNA and protein within the distinct regions of the Sprague-Dawley rat kidney (i.e., the cortex and outer and inner medulla) during 24 and 48 h of water restriction. A competitive reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to quantify changes in the V2R mRNA, in which a deletion mutant RNA transcript was used to control for the efficiency of RT-PCR. Western blot analysis was utilized for the quantification of the V2R protein. The results showed that the steady-state levels of the V2R mRNA decreased in a time-dependent manner in the cortex and outer and inner medulla throughout 48 h of water restriction. Western blot analysis revealed that the V2R protein in the renal cortex decreased after the initial 24 h of water restriction and remained decreased at 48 h. In contrast, outer medullary V2R protein decreased significantly only after 48 h of water restriction, whereas no significant change in the inner medullary V2R protein was observed throughout the 48 h of water restriction. These results suggest that water restriction leads to a regional time-dependent downregulation of the V2R mRNA and protein within the rat kidney. The stability of the plasma membrane V2R protein within the inner medulla may allow for the optimization of urine concentration and minimize water loss during periods of water restriction.

scholarly journals Nanoscopic investigation of C9orf72 poly-GA oligomers on nuclear membrane disruption by a photoinducible platform

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Hung-Ming Chien ◽  
Ruei-Yu He ◽  
Chi-Chang Lee ◽  
Yung-An Huang ◽  
I-Ju Hung ◽  
...  
Keyword(s):  
Nuclear Membrane ◽  
Cortical Neurons ◽  
Dna Binding Protein ◽  
Nucleocytoplasmic Transport ◽  
Time Dependent ◽  
Membrane Disruption ◽  
Dependent Manner ◽  
Alanine Dipeptide ◽  
Lateral Sclerosis ◽  
In Cells

AbstractGlycine-alanine dipeptide repeats (GA DPRs) translated from the mutated C9orf72 gene have recently been correlated with amyotrophic lateral sclerosis (ALS). While GA DPRs aggregates have been suggested as amyloid, the biophysical features and cytotoxicity of GA DPRs oligomers has not been explored due to its unstable nature. In this study, we develop a photoinducible platform based on methoxynitrobenzene chemistry to enrich GA DPRs that allows monitoring the oligomerization process of GA DPRs in cells. By applying advanced microscopies, we examined the GA DPRs oligomerization process nanoscopically in a time-dependent manner. We provided direct evidences to demonstrate GA DPRs oligomers rather than nanofibrils disrupt nuclear membrane. Moreover, we found GA DPRs hamper nucleocytoplasmic transport in cells and cause cytosolic retention of TAR DNA-binding protein 43 in cortical neurons. Our results highlight the toxicity of GA DPRs oligomers, which is a key step toward elucidating the pathological roles of C9orf72 DPRs.

Vas deferens epithelial cells in subculture: a model to study androgen regulation of gene expression

1995 ◽  
Vol 15 (2) ◽  
pp. 129-141 ◽  
Author(s):  
A Dassouli ◽  
Ch Darne ◽  
S Fabre ◽  
M Manin ◽  
G Veyssière ◽  
...  
Keyword(s):  
Gene Expression ◽  
Androgen Receptor ◽  
Epithelial Cells ◽  
Vas Deferens ◽  
Regulatory Elements ◽  
Receptor Expression ◽  
Time Dependent ◽  
Dependent Manner ◽  
Regulated Gene Expression ◽  
Cat Gene

ABSTRACT The understanding of androgen-regulated gene expression requires a cell culture system that mimics the functions of cells in vivo. In the present paper we have examined a vas deferens epithelial cell subculture system. Cultured vas deferens epithelial cells have been shown to exhibit polarized properties characteristic of functioning epithelia and to display a high level of androgen receptors. Incubation of cells with androgen caused a decrease in cellular androgen receptor mRNA that was time-dependent. Total suppression was observed after 24 h of exposure to androgen. By contrast, incubation of vas deferens epithelial cells with androgen resulted in a threefold increase in the cellular content of androgen receptor protein, as assayed by ligand binding. In response to androgens, vas deferens epithelial cells expressed mouse vas deferens protein mRNA (MVDP mRNA). Maximum expression of the MVDP gene, at both mRNA and protein levels, was observed after 24 h of androgen induction. DEAE-dextran transfection conditions were defined using the MMTV-CAT vector. Dihydrotestosterone stimulated the transcription activation of MMTV-CAT gene in vas deferens epithelial cells in a dose- and time-dependent manner. No induction was seen when fragments of the MVDP promoter region were cloned directly in front of the CAT gene and transiently transfected into vas deferens epithelial cells. It was found that cotransfection of cells with MVDP-CAT constructs and with an androgen receptor expression vector resulted in a small but consistent androgen-dependent increase in reporter gene activity. Transiently transfected vas deferens epithelial cells are a suitable model with which to study the effect of androgen on gene regulatory elements.

The Natural Flavonoid Naringenin Inhibits the Cell Growth of WilmsTumorinChildren by Suppressing TLR4/NF-κB Signaling

2020 ◽  
Vol 20 ◽  
Author(s):  
Hongtao Li ◽  
Peng Chen ◽  
Lei Chen ◽  
Xinning Wang
Keyword(s):  
Cell Growth ◽  
Western Blot ◽  
Wilms Tumor ◽  
Critical Role ◽  
Time Dependent ◽  
Luciferase Assay ◽  
Dependent Manner ◽  
Tlr4 Expression ◽  
Protein Levels

Background: Nuclear factor kappa B (NF-κB) is usually activated in Wilms tumor (WT) cells and plays a critical role in WT development. Objective: The study purpose was to screen a NF-κB inhibitor from natural product library and explore its effects on WT development. Methods: Luciferase assay was employed to assess the effects of natural chemical son NF-κB activity. CCK-8 assay was conducted to assess cell growth in response to naringenin. WT xenograft model was established to analyze the effect of naringenin in vivo. Quantitative real-time PCR and Western blot were performed to examine the mRNA and protein levels of relative genes, respectively. Results: Naringenin displayed significant inhibitory effect on NF-κB activation in SK-NEP-1 cells. In SK-NEP-1 and G-401 cells, naringenin inhibited p65 phosphorylation. Moreover, naringenin suppressed TNF-α-induced p65 phosphorylation in WT cells. Naringenin inhibited TLR4 expression at both mRNA and protein levels in WT cells. CCK-8 staining showed that naringenin inhibited cell growth of the two above WT cells in dose-and time-dependent manner, whereas Toll-like receptor 4 (TLR4) over expression partially reversed the above phenomena. Besides, naringenin suppressed WT tumor growth in dose-and time-dependent manner in vivo. Western blot found that naringenin inhibited TLR4 expression and p65 phosphorylation in WT xenograft tumors. Conclusion: Naringenin inhibits WT development viasuppressing TLR4/NF-κB signaling

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