scholarly journals Radiosynthesis and in Vivo and ex Vivo Evaluation of Isomeric [11C]methoxy Analogs of Nimesulide as Brain Cyclooxygenase-2-Targeted Imaging Agents

2022 ◽  
Vol 45 (1) ◽  
pp. 94-103
Author(s):  
Yumi Yamamoto ◽  
Tetsuro Tago ◽  
Jun Toyohara ◽  
Yohei Saito ◽  
Fumihiko Yamamoto
Keyword(s):  
Ex Vivo ◽  
Cyclooxygenase 2 ◽  
Imaging Agents ◽  
Targeted Imaging

Correlation between GC-C expression and uptake of GC-C-targeted imaging agents in vitro and in vivo

2010 ◽  
Vol 37 (6) ◽  
pp. 710
Author(s):  
Dijie Liu ◽  
Leonard R. Forte ◽  
Michael F. Giblin
Keyword(s):  
Imaging Agents ◽  
Targeted Imaging

scholarly journals Supramolecular Encapsulation of Small-Ultra Red Fluorescent Proteins in Virus-Like Nanoparticles for Non-Invasive In Vivo Imaging Agents

2020 ◽  
Author(s):  
Fabian C. Herbert ◽  
Olivia Brohlin ◽  
Tyler Galbraith ◽  
Candace Benjamin ◽  
Cesar A. Reyes ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Ex Vivo ◽  
Imaging Agents ◽  
Non Invasive ◽  
Red Fluorescent Proteins ◽  
Ex Vivo Imaging

<div> <div> <div> <p>Icosahedral virus-like particles (VLPs) derived from bacteriophages Qβ and PP7 encapsulating small-ultra red fluorescent protein (smURFP) were produced using a versatile supramolecualr capsid dissassemble-reassemble approach. The generated fluorescent VLPs display identical structural properties to their non-fluorescent analogs. Encapsulated smURFP shows indistinguishable photochemical properties to its unencapsulated counterpart, exhibits outstanding stability towards pH, and produces bright in vitro images following phagocytosis by macrophages. In vivo imaging allows biodistribution to be imaged at different time points. Ex vivo imaging of intravenously administered encapsulated smURFP reveleas localization in the liver and </p> </div> </div> <div> <div> <p>kidneys after 2 h blood circulation and substantial elimination constructs as non-invasive in vivo imaging agents. </p> </div> </div> </div>

scholarly journals Cyclooxygenase-2 inhibition reduces stress-induced affective pathology

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Joyonna Carrie Gamble-George ◽  
Rita Baldi ◽  
Lindsay Halladay ◽  
Adrina Kocharian ◽  
Nolan Hartley ◽  
...  
Keyword(s):  
Anxiety Disorders ◽  
Basolateral Amygdala ◽  
Cannabinoid Receptor ◽  
Ex Vivo ◽  
Receptor Activation ◽  
Cyclooxygenase 2 ◽  
Novel Treatment ◽  
Cox 2 ◽  
Cox 2 Inhibitors

Mood and anxiety disorders are the most prevalent psychiatric conditions and are exacerbated by stress. Recent studies have suggested cyclooxygenase-2 (COX-2) inhibition could represent a novel treatment approach or augmentation strategy for affective disorders including anxiety disorders and major depression. We show that traditional COX-2 inhibitors and a newly developed substrate-selective COX-2 inhibitor (SSCI) reduce a variety of stress-induced behavioral pathologies in mice. We found that these behavioral effects were associated with a dampening of neuronal excitability in the basolateral amygdala (BLA) ex vivo and in vivo, and were mediated by small-conductance calcium-activated potassium (SK) channel and CB1 cannabinoid receptor activation. Taken together, these data provide further support for the potential utility of SSCIs, as well as traditional COX-2 inhibitors, as novel treatment approaches for stress-related psychiatric disorders.

scholarly journals Feedback regulation of cyclooxygenase-2 transcription ex vivo and in vivo

2009 ◽  
Vol 378 (3) ◽  
pp. 534-538 ◽  
Author(s):  
Tomo-o Ishikawa ◽  
Naveen Jain ◽  
Harvey R. Herschman
Keyword(s):  
Ex Vivo ◽  
Feedback Regulation ◽  
Cyclooxygenase 2

scholarly journals Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents

2016 ◽  
Vol 57 (4) ◽  
pp. 640-645 ◽  
Author(s):  
D. Soodgupta ◽  
H. Zhou ◽  
W. Beaino ◽  
L. Lu ◽  
M. Rettig ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Ex Vivo ◽  
Imaging Agents ◽  
In Vivo Evaluation

scholarly journals Supramolecular Encapsulation of Small-Ultra Red Fluorescent Proteins in Virus-Like Nanoparticles for Non-Invasive In Vivo Imaging Agents

2020 ◽  
Author(s):  
Fabian C. Herbert ◽  
Olivia Brohlin ◽  
Tyler Galbraith ◽  
Candace Benjamin ◽  
Cesar A. Reyes ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Ex Vivo ◽  
Imaging Agents ◽  
Non Invasive ◽  
Red Fluorescent Proteins ◽  
Ex Vivo Imaging

<div> <div> <div> <p>Icosahedral virus-like particles (VLPs) derived from bacteriophages Qβ and PP7 encapsulating small-ultra red fluorescent protein (smURFP) were produced using a versatile supramolecualr capsid dissassemble-reassemble approach. The generated fluorescent VLPs display identical structural properties to their non-fluorescent analogs. Encapsulated smURFP shows indistinguishable photochemical properties to its unencapsulated counterpart, exhibits outstanding stability towards pH, and produces bright in vitro images following phagocytosis by macrophages. In vivo imaging allows biodistribution to be imaged at different time points. Ex vivo imaging of intravenously administered encapsulated smURFP reveleas localization in the liver and </p> </div> </div> <div> <div> <p>kidneys after 2 h blood circulation and substantial elimination constructs as non-invasive in vivo imaging agents. </p> </div> </div> </div>

scholarly journals Preparation of multifunctional nanobubbles and their application in bimodal imaging and targeted combination therapy of early pancreatic cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hengli Yang ◽  
Ping Zhao ◽  
Yonggang Zhou ◽  
Qiaoying Li ◽  
Wenbin Cai ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Ex Vivo ◽  
Combined Treatment ◽  
Tunel Staining ◽  
Targeted Imaging ◽  
Dual Mode ◽  
Pancreatic Cancer Cells ◽  
Bimodal Imaging

AbstractPancreatic cancer will gradually become the second leading cause of cancer death due to its poor suitability for surgical treatment, frequent recurrence and metastasis, and insensitivity to radiotherapy and chemotherapy. Strategies for precise early detection and effective targeted treatment of pancreatic cancer are urgently needed. Because of its unique advantages, molecular targeted contrast-enhanced ultrasound imaging (CEUI) has generated new opportunities to overcome this challenge. The aim of this study was to explore multifunctional nanobubbles named IR780-NBs-DTX as novel ultrasound contrast agents (UCAs) for dual-mode targeted imaging and photothermal ablation combined with chemotherapy for pancreatic cancer. An optimized “film hydration method” was used to prepare IR780-NBs-DTX in this research. The characteristics and ability of the new UCAs were detected via in vitro, in vivo and ex vivo experiments. The initial dose of 0.15 mg IR-780 iodide/1.0 mg DTX was considered to be the best formula for IR780-NBs-DTX, and the concentration of 6 ×106 bubbles/mL was best for CEUI. The excellent characteristics of IR780-NBs-DTX, including a uniform nanoscale particle size (349.8± 159.1 nm, n= 3), good performance in dual-mode imaging, high stability and reliable biocompatibility, were also proven. In the in vitro cell experiments, IR780-NBs-DTX targeted more pancreatic cancer cells than the control treatments, and the targeting rate was approximately 95.6± 1.7%. Under irradiation with an 808 nm laser, most cells died. Furthermore, the in vivo study demonstrated that IR780-NBs-DTX could precisely detect pancreatic cancer through near infrared fluorescence (NIRF) imaging and CEUI, and the tumor almost disappeared at 18 days after combined treatment. In ex vivo experiments, immunohistochemistry (IHC) and immunofluorescence (IF) showed that the expression of HSP70 increased and that of PCNA decreased, and many apoptotic tumor cells were observed by TUNEL staining in the IR780-NBs-DTX group. The newly prepared IR780-NBs-DTX are novel nanosized UCAs with high efficiency for dual-mode molecular targeted imaging and combined therapy, and they may have future potential applications in the precise detection and effective targeted therapy of small and metastatic lesions in the early stage of pancreatic cancer.

scholarly journals In Silico, In Vitro, and In Vivo Analysis of Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza as Modulators of Cyclooxygenase-2/mPGES-1/Endothelial Prostaglandin EP3 Pathway

Biomolecules ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 99
Author(s):  
Anella Saviano ◽  
Simona De Vita ◽  
Maria Giovanna Chini ◽  
Noemi Marigliano ◽  
Gianluigi Lauro ◽  
...  
Keyword(s):  
In Silico ◽  
Ex Vivo ◽  
Salvia Miltiorrhiza ◽  
Cyclooxygenase 2 ◽  
Tanshinone Iia ◽  
Prostaglandin E ◽  
Platelet Activity ◽  
Cox 2

Tanshinone IIA (TIIA) and cryptotanshinone (CRY) from Salvia miltiorrhiza Bunge were investigated for their inhibitory activity against the cyclooxygenase-2 (COX-2)/microsomal prostaglandin E synthase-1 (mPGES-1)/endothelial prostaglandin 3 (EP3) pathway using in silico, in vitro, in vivo, and ex vivo assays. From the analysis of the docking poses, both diterpenoids were able to interact significantly with COX-2, 5-lipoxygenase (5-LO), platelet-activating factor receptor (PAFR), and mPGES-1. This evidence was further corroborated by data obtained from a cell-free assay, where CRY displayed a significant inhibitory potency against mPGES-1 (IC50 = 1.9 ± 0.4 µM) and 5-LO (IC50 = 7.1 µM), while TIIA showed no relevant inhibition of these targets. This was consistent with their activity to increase mice bleeding time (CRY: 2.44 ± 0.13 min, p ≤ 0.001; TIIA: 2.07 ± 0.17 min p ≤ 0.01) and with the capability to modulate mouse clot retraction (CRY: 0.048 ± 0.011 g, p ≤ 0.01; TIIA: 0.068 ± 0.009 g, p ≤ 0.05). For the first time, our results show that TIIA and, in particular, CRY are able to interact significantly with the key proteins involved not only in the onset of inflammation but also in platelet activity (and hyper-reactivity). Future preclinical and clinical investigations, together with this evidence, could provide the scientific basis to consider these compounds as an alternative therapeutic approach for thrombotic- and thromboembolic-based diseases.

Folic acid-functionalized up-conversion nanoparticles: toxicity studies in vivo and in vitro and targeted imaging applications

Nanoscale ◽  
2014 ◽  
Vol 6 (15) ◽  
pp. 8878-8883 ◽  
Author(s):  
Lining Sun ◽  
Zuwu Wei ◽  
Haige Chen ◽  
Jinliang Liu ◽  
Jianjian Guo ◽  
...  
Keyword(s):  
Folic Acid ◽  
Hela Cells ◽  
Imaging Agents ◽  
Targeted Imaging ◽  
Nanoparticle Imaging ◽  
Good Biocompatibility ◽  
Low Cytotoxicity ◽  
Nanoparticles Toxicity

Three kinds of folate-targeted up-conversion nanoparticle imaging agents were prepared. These nanoparticles show good biocompatibility and up-conversion luminescence emission in water, and low cytotoxicity in vivo and in vitro. They were successfully applied to the targeted imaging of HeLa cells.

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