scholarly journals Circulating Inhibin A and Inhibin B in Normal Menstrual Cycle during Breeding Seasons of Japanese Monkeys

2002 ◽  
Vol 48 (4) ◽  
pp. 355-355 ◽  
Author(s):  
Keiko SHIMIZU ◽  
Chihiro KOJIMA ◽  
Masahiro KONDO ◽  
WanZhu JIN ◽  
Mariko ITO ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Inhibin B ◽  
Inhibin A ◽  
Japanese Monkeys ◽  
Normal Menstrual Cycle ◽  
Breeding Seasons

scholarly journals Secretion of inhibin A and inhibin B throughout pregnancy and the early postpartum period in chimpanzees

2001 ◽  
Vol 168 (2) ◽  
pp. 257-262 ◽  
Author(s):  
M Kondo ◽  
M Kondo ◽  
T Udono ◽  
WZ Jin ◽  
WZ Jin ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Postpartum Period ◽  
Plasma Concentrations ◽  
Late Pregnancy ◽  
Inhibin B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inhibin A ◽  
Early Postpartum Period ◽  
Early Postpartum ◽  
Normal Menstrual Cycle

Plasma concentrations of inhibin A and inhibin B during pregnancy and early lactation in chimpanzees were determined by enzyme-linked immunosorbent assay (ELISA). Plasma samples were taken from five pregnant chimpanzees at 6-9, 10, 20 and 25 weeks of pregnancy, and following parturition. Throughout pregnancy and the early postpartum period, circulating inhibin A and inhibin B concentrations remained low, at similar levels to those during the normal menstrual cycle in chimpanzees. Concentrations of inhibin A in the placental homogenate were high enough to be measured by the ELISA and by bioassay, whereas circulating inhibin bioactivities in late pregnancy were too low to be measured. Plasma concentrations of FSH remained low with no significant changes throughout pregnancy and the postpartum period. Plasma concentrations of oestradiol-17beta and progesterone at 25 weeks of pregnancy were much higher than normal menstrual cycle levels. It was concluded that in chimpanzees the levels of circulating inhibin A and inhibin B remained low throughout pregnancy and the early postpartum period, and that the concentrations of bioactive dimeric inhibin did not increase towards the end of pregnancy. The suppression of circulating FSH levels during pregnancy is suggested to be controlled by steroid hormones that increased significantly in late pregnancy, and the present findings further suggest that the secretory pattern and role of inhibin during pregnancy in chimpanzees may be different from that in human and other primates.

scholarly journals Human mammary gland and breast carcinoma contain immunoreactive inhibin/activin subunits: evidence for a secretion into cystic fluid

1999 ◽  
pp. 190-194 ◽  
Author(s):  
C Di Loreto ◽  
FM Reis ◽  
P Cataldi ◽  
C Zuiani ◽  
S Luisi ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Breast Carcinoma ◽  
Epithelial Cells ◽  
Activin A ◽  
Inhibin B ◽  
Fibrocystic Disease ◽  
Inhibin A ◽  
Intense Staining ◽  
Cystic Fluid ◽  
Breast Tissues

OBJECTIVE: Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. The present study investigated the localization of inhibin and activin subunits in human normal and pathological breast tissues. DESIGN: A cross-sectional study comparing the expression of inhibin/activin subunits alpha, betaA and betaB in surgical specimens from women undergoing reductive mammoplasty (classified, according to the phase of the menstrual cycle, as follicular, luteal, or postmenopausal), and patients submitted to lumpectomy for fibrocystic disease, benign (intraductal papilloma, adenomyoepithelioma, and hamartoma) or malignant breast neoplams (intraductal, intralobular, and invasive carcinoma). METHODS: Immunohistochemistry was used to localize inhibin alpha and activin betaA and betaB subunits in the cytoplasm of epithelial cells of mammary glands. Dimeric activin A, inhibin A and inhibin B were measured by specific two-site enzyme immunoassay in the cystic fluid collected from patients with fibrocystic disease. RESULTS: An intense staining for the alpha inhibin subunit and a mild staining for betaA and betaB subunits were present in samples obtained from normal breast tissue regardless of menstrual cycle phase, and in fibrocystic disease and benign neoplasms. Carcinoma cells stained weakly to moderately for alpha subunit and were negative for betaA and betaB subunits. Fibrocystic disease was associated with absence of betaA subunit expression in normal epithelial cells and intense staining for all subunits in the apocrine cells. Immunoreactive inhibin A, inhibin B, and activin A were also present in cystic fluid, suggesting a local secretion of these proteins. CONCLUSION: These data suggest a local expression and secretion of inhibin and activin in human normal, fibrocystic disease and neoplastic breast tissues. The low expression of these proteins may facilitate abnormal cell proliferation in breast carcinoma.

Secretion of Inhibin A and Inhibin B During Pregnancy and Early Postpartum Period in Japanese Monkeys

Endocrine ◽  
2002 ◽  
Vol 18 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Chihiro Kojima ◽  
Masahiro Kondo ◽  
WanZhu Jin ◽  
Keiko Shimizu ◽  
Mariko Itoh ◽  
...  
Keyword(s):  
Postpartum Period ◽  
Inhibin B ◽  
Inhibin A ◽  
Early Postpartum Period ◽  
Japanese Monkeys ◽  
Early Postpartum

SOME OBSERVATIONS ON PREGNANEDIOL EXCRETION DURING THE NORMAL MENSTRUAL CYCLE

1957 ◽  
Vol 24 (3_Suppl) ◽  
pp. S207
Author(s):  
A. Klopper
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
New Method ◽  
Menstrual Bleeding ◽  
Normal Menstrual Cycle ◽  
The Relationship ◽  
New View

Abstract The changes in view on the significance and amount of urinary pregnanediol in the menstrual cycle are reviewed; in particular the effects of the discovery that the adrenals in both sexes normally contribute to the urinary pregnanediol. Pregnanediol excretion during the menstrual cycle was studied by means of a new method of assay (Klopper et al., 1955) and the results applied to present day concepts of the growth and duration of the corpus luteum. The relationship between pregnanediol excretion and ovulation or the onset of menstrual bleeding was studied. A new view is put forward on the influence of age and parity on the production of progesterone by the corpus luteum.

Hormone levels in the normal menstrual cycle

Reproduction ◽  
1971 ◽  
Vol 27 (3) ◽  
pp. 481-484 ◽  
Author(s):  
J Newton ◽  
D Joyce ◽  
B Pearce ◽  
C Revell ◽  
J Tyler
Keyword(s):  
Menstrual Cycle ◽  
Hormone Levels ◽  
Normal Menstrual Cycle

Effectiveness of zona pellucida protein ZPB as an immunocontraceptive antigen

Reproduction ◽  
2000 ◽  
pp. 19-32 ◽  
Author(s):  
ML Martinez ◽  
JD Harris
Keyword(s):  
Menstrual Cycle ◽  
Recombinant Protein ◽  
Antibody Titre ◽  
Zona Pellucida ◽  
Protein A ◽  
Papio Cynocephalus ◽  
Cynomolgus Monkeys ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle

Immunization of female mammals with native zona pellucida (ZP) proteins is known to cause infertility. Since each human ZP protein is now available as a purified recombinant protein, is it possible to compare the immunocontraceptive potential of each ZP protein. A breeding study was conducted in cynomolgus monkeys (Macaca fasicularis) after immunization with recombinant human ZP (rhZP) proteins (ZPA, ZPB, ZPC) separately and in combinations. This study demonstrated that immunization with recombinant human ZPB (rhZPB) protein caused cynomolgus monkeys to become infertile for 9-35 months. A second study was conducted in baboons (Papio cynocephalus), which yielded a similar result. The baboons immunized with rhZPB became infertile for 9 to > 20 months. During the time of maximum antibody titre, some animals experienced disruption of the menstrual cycle, but eventually all of the animals resumed normal menstrual cycles. Control animals and animals immunized with other rhZP proteins all became pregnant before any of the rhZPB-treated animals. This is the first study in which a recombinant ZP protein has consistently induced infertility in a primate without permanent disruption of the normal menstrual cycle.

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