scholarly journals Microbial Community Composition of Polyhydroxyalkanoate-Accumulating Organisms in Full-Scale Wastewater Treatment Plants Operated in Fully Aerobic Mode

2013 ◽  
Vol 28 (1) ◽  
pp. 96-104 ◽  
Author(s):  
Mamoru Oshiki ◽  
Motoharu Onuki ◽  
Hiroyasu Satoh ◽  
Takashi Mino
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Community Composition ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Full Scale

Comparative analysis of DNA-based microbial community composition and substrate utilisation patterns of activated sludge microorganisms from wastewater treatment plants operated under different conditions

2010 ◽  
Vol 61 (11) ◽  
pp. 2843-2851 ◽  
Author(s):  
M. Matsuda ◽  
D. Inoue ◽  
Y. Anami ◽  
H. Tsutsui ◽  
K. Sei ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Carbon Source ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Rflp Analysis ◽  
Full Scale ◽  
Phylogenetic Composition ◽  
Carbon Source Utilisation

In this study, the microbial community structure and carbon source utilisation profile of activated sludge samples collected from full-scale municipal wastewater treatment plants (WWTPs) operated under different conditions were characterised and compared, respectively, using terminal-restriction fragment length polymorphism (T-RFLP) analysis and Biolog assay. Samples were collected from each biological treatment tank of six conventional activated sludge, two anaerobic–oxic, two anaerobic–anoxic–oxic, and one step-aeration processes in eight full-scale WWTPs in Osaka, Japan. Results of the T-RFLP analysis of eubacterial 16S rDNA showed that microbial communities of activated sludge differed greatly among samples, and that they were affected by process-based operational conditions. In contrast, the carbon source utilisation profiles of activated sludge samples were mutually similar, but appeared to be influenced slightly by aerated conditions at each reaction tank. Similar carbon source utilisation profiles among all samples suggest that the activated sludge community possesses functions that are necessary for wastewater treatment even if the phylogenetic composition is different. Different results from the T-RFLP analysis and Biolog assay suggest that the phylogenetic composition of microbial community might not necessarily reflect the wastewater treatment functions of the activated sludge.

scholarly journals Identification of the microbial community composition and structure of coal-mine wastewater treatment plants

2015 ◽  
Vol 175 ◽  
pp. 1-5 ◽  
Author(s):  
Qiao Ma ◽  
Yuan-Yuan Qu ◽  
Xu-Wang Zhang ◽  
Wen-Li Shen ◽  
Zi-Yan Liu ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Community Composition ◽  
Coal Mine ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Composition And Structure ◽  
Mine Wastewater

scholarly journals Do initial concentration and activated sludge seasonality affect pharmaceutical biotransformation rate constants?

2021 ◽  
Author(s):  
Tamara J. H. M. van Bergen ◽  
Ana B. Rios-Miguel ◽  
Tom M. Nolte ◽  
Ad M. J. Ragas ◽  
Rosalie van Zelm ◽  
...  
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Community Composition ◽  
Rate Constants ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Amplicon Sequencing ◽  
Rrna Gene ◽  
Initial Concentration ◽  
Different Seasons

Abstract Pharmaceuticals find their way to the aquatic environment via wastewater treatment plants (WWTPs). Biotransformation plays an important role in mitigating environmental risks; however, a mechanistic understanding of involved processes is limited. The aim of this study was to evaluate potential relationships between first-order biotransformation rate constants (kb) of nine pharmaceuticals and initial concentration of the selected compounds, and sampling season of the used activated sludge inocula. Four-day bottle experiments were performed with activated sludge from WWTP Groesbeek (The Netherlands) of two different seasons, summer and winter, spiked with two environmentally relevant concentrations (3 and 30 nM) of pharmaceuticals. Concentrations of the compounds were measured by LC–MS/MS, microbial community composition was assessed by 16S rRNA gene amplicon sequencing, and kb values were calculated. The biodegradable pharmaceuticals were acetaminophen, metformin, metoprolol, terbutaline, and phenazone (ranked from high to low biotransformation rates). Carbamazepine, diatrizoic acid, diclofenac, and fluoxetine were not converted. Summer and winter inocula did not show significant differences in microbial community composition, but resulted in a slightly different kb for some pharmaceuticals. Likely microbial activity was responsible instead of community composition. In the same inoculum, different kb values were measured, depending on initial concentration. In general, biodegradable compounds had a higher kb when the initial concentration was higher. This demonstrates that Michealis-Menten kinetic theory has shortcomings for some pharmaceuticals at low, environmentally relevant concentrations and that the pharmaceutical concentration should be taken into account when measuring the kb in order to reliably predict the fate of pharmaceuticals in the WWTP. Key points • Biotransformation and sorption of pharmaceuticals were assessed in activated sludge. • Higher initial concentrations resulted in higher biotransformation rate constants for biodegradable pharmaceuticals. • Summer and winter inocula produced slightly different biotransformation rate constants although microbial community composition did not significantly change. Graphical abstract

scholarly journals The impact of immigration on microbial community composition in full-scale anaerobic digesters

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Rasmus H. Kirkegaard ◽  
Simon J. McIlroy ◽  
Jannie M. Kristensen ◽  
Marta Nierychlo ◽  
Søren M. Karst ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Composition ◽  
Microbial Community Composition ◽  
Full Scale ◽  
Anaerobic Digesters ◽  
The Impact

scholarly journals Ultraviolet disinfection impacts the microbial community composition and function of treated wastewater effluent and the receiving urban river

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7455 ◽  
Author(s):  
Imrose Kauser ◽  
Mark Ciesielski ◽  
Rachel S. Poretsky
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
River Water ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
The United States ◽  
Treated Wastewater ◽  
Coliform Bacteria ◽  
Uv Disinfection ◽  
Treated Effluent

Background In the United States, an estimated 14,748 wastewater treatment plants (WWTPs) provide wastewater collection, treatment, and disposal service to more than 230 million people. The quality of treated wastewater is often assessed by the presence or absence of fecal indicator bacteria. UV disinfection of wastewater is a common final treatment step used by many wastewater treatment plants in order to reduce fecal coliform bacteria and other pathogens; however, its potential impacts on the total effluent bacterial community are seemingly varied. This is especially important given that urban WWTPs typically return treated effluent to coastal and riverine environments and thus are a major source of microorganisms, genes, and chemical compounds to these systems. Following rainfall, stormflow conditions can result in substantial increases to effluent flow into combined systems. Methods Here, we conducted a lab-scale UV disinfection on WWTP effluent using UV dosage of 100 mJ/cm2 and monitored the active microbiome in UV-treated effluent and untreated effluent over the course of 48 h post-exposure using 16S rRNA sequencing. In addition, we simulated stormflow conditions with effluent UV-treated and untreated effluent additions to river water and compared the microbial communities to those in baseflow river water. We also tracked the functional profiles of genes involved in tetracycline resistance (tetW) and nitrification (amoA) in these microcosms using RT-qPCR. Results We showed that while some organisms, such as members of the Bacteroidetes, are inhibited by UV disinfection and overall diversity of the microbial community decreases following treatment, many organisms not only survive, but remain active. These include common WWTP-derived organisms such as Comamonadaceae and Pseudomonas. When combined with river water to mimic stormflow conditions, these organisms can persist in the environment and potentially enhance microbial functions such as nitrification and antibiotic resistance.

scholarly journals Identifying the abundant and active microorganisms common to full-scale anaerobic digesters

2017 ◽  
Author(s):  
Rasmus H. Kirkegaard ◽  
Simon J. McIlroy ◽  
Jannie M. Kristensen ◽  
Marta Nierychlo ◽  
Søren M. Karst ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Amplicon Sequencing ◽  
Full Scale ◽  
Rrna Gene ◽  
Anaerobic Digesters ◽  
Abundant Otus ◽  
Source Of Information

AbstractAnaerobic digestion is widely applied to treat organic waste at wastewater treatment plants. Characterisation of the underlying microbiology represents a source of information to develop strategies for improved operation. To this end, we investigated the microbial community composition of thirty-two full-scale digesters over a six-year period using 16S rRNA gene amplicon sequencing. Sampling of the sludge fed into these systems revealed that several of the most abundant populations were likely inactive and immigrating with the influent. This observation indicates that a failure to consider immigration will interfere with correlation analysis and give an inaccurate picture of the active microbial community. Furthermore, several abundant OTUs could not be classified to genus level with commonly applied taxonomies, making inference of their function unreliable. As such, the existing MiDAS taxonomy was updated to include these abundant phylotypes. The communities of individual plants surveyed were remarkably similar – with only 300 OTUs representing 80% of the total reads across all plants, and 15% of these identified as likely inactive immigrating microbes. By identifying the abundant and active taxa in anaerobic digestion, this study paves the way for targeted characterisation of the process important organisms towards an in-depth understanding of the microbial ecology of these biotechnologically important systems.

scholarly journals Data on microbial community composition of sludge from high altitude wastewater treatment plants determined by 16S rRNA gene sequencing

Data in Brief ◽  
2019 ◽  
Vol 23 ◽  
pp. 103739 ◽  
Author(s):  
Chen Chen ◽  
Xiaochun Peng ◽  
Shan Huang ◽  
Yuannan Wang ◽  
Shumei Liao ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Rrna Gene Sequencing

Wastewater treatment plant effluent introduces recoverable shifts in microbial community composition in receiving streams

2018 ◽  
Vol 613-614 ◽  
pp. 1104-1116 ◽  
Author(s):  
Jacob R. Price ◽  
Sarah H. Ledford ◽  
Michael O. Ryan ◽  
Laura Toran ◽  
Christopher M. Sales
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Community Composition ◽  
Wastewater Treatment Plant ◽  
Microbial Community Composition ◽  
Treatment Plant ◽  
Wastewater Treatment Plant Effluent
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