The Relation between Serum Cytokine Levels and Common Laboratory Tests in Healthy Japanese Males

SummaryThrombelastography, although proven as a useful research tool has not been evaluated for its clinical utility against common coagulation laboratory tests. In this study we compare the thrombelastographic measurements with six common tests (the hematocrit, platelet count, fibrinogen, prothrombin time, activated thromboplastin time and fibrin split products). For such comparisons, two samples of subjects were selected, 141 normal volunteers and 121 patients with cancer. The data was subjected to various statistical techniques such as correlation, ANOVA, canonical and discriminant analysis to measure the extent of the correlations between the two sets of variables and their relative strength to detect blood clotting abnormalities. The results indicate that, although there is a strong relationship between the thrombelastographic variables and these common laboratory tests, the thrombelastographic variables contain additional information on the hemostatic process.

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An Examination of Generalized Pustular Psoriasis in Pregnancy Based on the Serum Cytokine Levels.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.61.144 ◽

1999 ◽

Vol 61 (2) ◽

pp. 144-147

Author(s):

Miyako KUDO ◽

Yoshihiro UMEBAYASHI ◽

Tomoe KOTSUJI ◽

Fujio OTSUKA

Keyword(s):

Pustular Psoriasis ◽

Serum Cytokine ◽

Cytokine Levels ◽

Generalized Pustular Psoriasis ◽

In Pregnancy

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Evaluation and cost estimation of laboratory tests overuse through a CCDSS in a university hospital

European Journal of Public Health ◽

10.1093/eurpub/ckaa165.221 ◽

2020 ◽

Vol 30 (Supplement_5) ◽

Author(s):

D Vallone ◽

A Tamburrano ◽

C Carrozza ◽

A Urbani ◽

A Cambieri ◽

...

Keyword(s):

Cost Estimation ◽

Laboratory Tests ◽

Quality Care ◽

Clinical Decision Support Systems ◽

Patient Characteristics ◽

Clinical Decision ◽

University Hospital ◽

Specific Patient ◽

Global Rate ◽

Common Laboratory

Abstract Computerized Clinical Decision Support Systems (CCDSS) are information technology-based systems that use specific patient characteristics and combine them with rule-based algorithms. The aim of this study is to conduct a survey to measure and assess the over-utilization rates of laboratory requests and to estimate the monthly cost of inappropriate requests in inpatients of the “Fondazione Policlinico Universitario A. Gemelli IRCCS” Care Units. This observational study is based on the count of rules violations for 43 different types of laboratory tests requested by the Hospital physicians, for a total of 5,716,370 requests, over a continuous period of 20 months (from 1 July 2016 to 28 February 2018). Requests from all the hospital internal departments (except for Emergency, Intensive Care Units and Urgent requests) were monitored. The software intercepted and counted, in silent mode for the operator, all requests and violations for each laboratory test among those identified. During the observation period a mean of 285,819 requests per month were analyzed and 40,462 violations were counted. The global rate of overuse was 15.2% ± 3.0%. The overall difference among sub-groups was significant (p < 0.001). The most inappropriate exams were Alpha Fetoprotein (85.8% ± 30.5%), Chlamydia trachomatis PCR (48.7% ± 8.8%) and Alkaline Phosphatase (20.3% ± 6.5%). All the exams, globally considered, generated an estimated avoidable cost of 1,719,337€ (85,967€ per month) for the hospital. This study reports rates (15.2%) similar to other works. The real impact of inappropriateness is difficult to assess, but the generated costs for patients, hospitals and health systems are certainly high and not negligible. Key messages It would be desirable for international medical communities to produce a complete panel of prescriptive rules for all the most common laboratory exam. That is useful not only to reduce costs, but also to ensure standardization and high-quality care.

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Reactive arthritis and undifferentiated peripheral spondyloarthritis share human leucocyte antigen B27 subtypes and serum and synovial fluid cytokine profiles

Rheumatology ◽

10.1093/rheumatology/keaa746 ◽

2020 ◽

Author(s):

Jyoti Ranjan Parida ◽

Sandeep Kumar ◽

Sakir Ahmed ◽

Smriti Chaurasia ◽

Ratnadeep Mukherjee ◽

...

Keyword(s):

Statistical Difference ◽

Human Leucocyte Antigen ◽

Healthy Controls ◽

Hla B27 ◽

Serum Cytokine ◽

Cytokine Profiles ◽

Cytokine Levels ◽

Peripheral Spondyloarthritis ◽

Multiplex Cytokine ◽

Age And Sex

Abstract Objectives Peripheral SpA (pSpA) is comprised of ReA, PsA, enteritis-associated arthritis and undifferentiated pSpA (upSpA). ReA and upSpA share T cell oligotypes and metabolomics in serum and SF. We investigated HLA-B27 subtypes and cytokines in serum and SF that were compared between ReA and upSpA. Methods ReA and upSpA were compared in two cohorts. In cohort I (44 ReA and 56 upSpA), HLA-B27 subtyping was carried out. In cohort II (17 ReA and 21 upSpA), serum and SF cytokines were compared using a multiplex cytokine bead assay (27 cytokines). A total of 28 healthy controls with similar age and sex to cohort II were included for comparison of serum cytokine levels. Results In cohort I, HLA-B27 was positive in 81.8% (36/44) of ReA and 85.71% (48/56) of upSpA patients. HLA-B27 typing was successful in 70 patients (30 ReA and 40 uSpA). HLA-B*2705 was the most common, followed by HLA-B*2704 and HLA-B*2707. Frequencies were the same between ReA and upSpA. In cohort II, 14 cytokines were detectable in the serum of patients. The levels of eight cytokines were higher than in the controls. The cytokine levels of ReA and upSpA were similar. Sixteen cytokines were detectable in the SF of patients. There was no statistical difference in the levels between ReA and upSpA. The cytokine profiles in sera and SF were also similar among HLA-B27-positive and negative patients. Conclusion ReA and upSpA have similar HLA-B27 subtype associations and similar cytokine profiles. They should be considered as a single entity during studies as well as clinical management.

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