A Quantitative Analysis of the Literature on Plant Heavy Metal Transporters Based on WoS Citation Database

2021 ◽  
Vol 10 (05) ◽  
pp. 613-626
Author(s):  
叶清 何
Keyword(s):  
Heavy Metal ◽  
Quantitative Analysis ◽  
Metal Transporters ◽  
Citation Database

scholarly journals The Role of Aquaporin Overexpression in the Modulation of Transcription of Heavy Metal Transporters under Cadmium Treatment in Poplar

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 54
Author(s):  
Andrea Neri ◽  
Silvia Traversari ◽  
Andrea Andreucci ◽  
Alessandra Francini ◽  
Luca Sebastiani
Keyword(s):  
Heavy Metal ◽  
Transcriptional Response ◽  
Metal Transporters ◽  
Metal Transporter ◽  
Cadmium Treatment ◽  
Tolerance To Cadmium ◽  
Different Response ◽  
Cd Translocation ◽  
Hydroponic Conditions

Populus alba ‘Villafranca’ clone is well-known for its tolerance to cadmium (Cd). To determine the mechanisms of Cd tolerance of this species, wild-type (wt) plants were compared with transgenic plants over-expressing an aquaporin (aqua1, GenBank GQ918138). Plants were maintained in hydroponic conditions with Hoagland’s solution and treated with 10 µM of Cd, renewed every 5 d. The transcription levels of heavy metal transporter genes (PaHMA2, PaNRAMP1.3, PaNRAMP2, PaNRAMP3.1, PaNRAMP3.2, PaABCC9, and PaABCC13) were analyzed at 1, 7, and 60 d of treatment. Cd application did not induce visible toxicity symptoms in wt and aqua1 plants even after 2 months of treatment confirming the high tolerance of this poplar species to Cd. Most of the analyzed genes showed in wt plants a quick response in transcription at 1 d of treatment and an adaptation at 60 d. On the contrary, a lower transcriptional response was observed in aqua1 plants in concomitance with a higher Cd concentration in medial leaves. Moreover, PaHMA2 showed at 1 d an opposite trend within organs since it was up-regulated in root and stem of wt plants and in leaves of aqua1 plants. In summary, aqua1 overexpression in poplar improved Cd translocation suggesting a lower Cd sensitivity of aqua1 plants. This different response might be due to a different transcription of PaNRAMP3 genes that were more transcribed in wt line because of the importance of this gene in Cd compartmentalization.

scholarly journals The effectiveness of laser-induced breakdown spectroscopy (LIBS) as a quantitative tool for environmental characterization

2021 ◽  
Author(s):  
Elizabeth Corriveau ◽  
Ashley Mossell ◽  
Holly VerMeulen ◽  
Samuel Beal ◽  
Jay Clausen
Keyword(s):  
Heavy Metal ◽  
Quantitative Analysis ◽  
Homeland Security ◽  
Inductively Coupled Plasma ◽  
Low Cost ◽  
Emission Spectrometry ◽  
Laser Induced Breakdown Spectroscopy ◽  
Cold Temperatures ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown

Laser-induced breakdown spectroscopy (LIBS) is a rapid, low-cost analytical method with potential applications for quantitative analysis of soils for heavy metal contaminants found in military ranges. The Department of Defense (DoD), Army, and Department of Homeland Security (DHS) have mission requirements to acquire the ability to detect and identify chemicals of concern in the field. The quantitative potential of a commercial off-the-shelf (COTS) hand-held LIBS device and a classic laboratory bench-top LIBS system was examined by measuring heavy metals (antimony, tungsten, iron, lead, and zinc) in soils from six military ranges. To ensure the accuracy of the quantified results, we also examined the soil samples using other hand-held and bench-top analytical methods, to include Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) and X-Ray Fluorescence (XRF). The effects of soil heterogeneity on quantitative analysis were reviewed with hand-held and bench-top systems and compared multivariate and univariate calibration algorithms for heavy metal quantification. In addition, the influence of cold temperatures on signal intensity and resulting concentration were examined to further assess the viability of this technology in cold environments. Overall, the results indicate that additional work should be performed to enhance the ability of LIBS as a reliable quantitative analytical tool.

scholarly journals Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses

2021 ◽  
Vol 22 (24) ◽  
pp. 13583
Author(s):  
Siyu Qiao ◽  
Ye Tao ◽  
Qinghua Shan ◽  
Jingang Wang ◽  
Tuanyao Chai ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Heavy Metal Contamination ◽  
The Other ◽  
Blue Dye ◽  
Metal Transporters ◽  
Genes Encoding ◽  
Total Antioxidant ◽  
Physiological Indexes

Heavy metals negatively affect soil quality and crop growth. In this study, we compared the tolerance of six ryegrass cultivars to cobalt (Co2+), lead (Pb2+), and nickel (Ni2+) stresses by analyzing their physiological indexes and transcript levels of genes encoding metal transporters. Compared with the other cultivars, the cultivar Lm1 showed higher germination rates and better growth under Co2+, Pb2+, or Ni2+ treatments. After 48 h of Co2+ treatment, the total antioxidant capacity of all six ryegrass cultivars was significantly increased, especially that of Lm1. In contrast, under Pb2+ stress, total antioxidant capacity of five cultivars was significantly decreased, but that of Lm1 was unaffected at 24 h. Staining with Evans blue dye showed that the roots of Lm1 were less injured than were roots of the other five ryegrass cultivars by Co2+, Pb2+, and Ni2+. Lm1 translocated and accumulated lesser Co2+, Pb2+, and Ni2+ than other cultivars. In Lm1, genes encoding heavy metal transporters were differentially expressed between the shoots and roots in response to Co2+, Pb2+, and Ni2+. The aim of these researches could help find potential resource for phytoremediation of heavy metal contamination soil. The identified genes related to resistance will be useful targets for molecular breeding.

scholarly journals Heavy Metal Transporters-Associated Proteins in Solanum tuberosum: Genome-Wide Identification, Comprehensive Gene Feature, Evolution and Expression Analysis

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1269
Author(s):  
Guandi He ◽  
Lijun Qin ◽  
Weijun Tian ◽  
Lulu Meng ◽  
Tengbing He ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Abiotic Stress ◽  
Solanum Tuberosum ◽  
Complex Signal ◽  
Metal Transporters ◽  
Cis Acting ◽  
Transporter Family ◽  
Associated Proteins ◽  
Gene Structures ◽  
Underlying Mechanisms

Plants have evolved a number of defense and adaptation responses to protect themselves against challenging environmental stresses. Genes containing a heavy metal associated (HMA) domain are required for the spatiotemporal transportation of metal ions that bind with various enzymes and co-factors within the cell. To uncover the underlying mechanisms mediated by StHMA genes, we identified 36 gene members in the StHMA family and divided them into six subfamilies by phylogenetic analysis. The StHMAs had high collinearity and were segmentally duplicated. Structurally, most StHMAs had one HMA domain, StHIPPc and StRNA1 subfamilies had two, and 13 StHMAs may be genetically variable. The StHMA gene structures and motifs varied considerably among the various classifications, this suggests the StHMA family is diverse in genetic functions. The promoter analysis showed that the StHMAs had six main cis-acting elements with abiotic stress. An expression pattern analysis revealed that the StHMAs were expressed tissue specifically, and a variety of abiotic stresses may induce the expression of StHMA family genes. The HMA transporter family may be regulated and expressed by a series of complex signal networks under abiotic stress. The results of this study may help to establish a theoretical foundation for further research investigating the functions of HMA genes in Solanum tuberosum to elucidate their regulatory role in the mechanism governing the response of plants to abiotic stress.

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