scholarly journals Quantification of cancer cell migration with an integrated experimental-computational pipeline

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1296 ◽  
Author(s):  
Edwin F Juarez ◽  
Carolina Garri ◽  
Ahmadreza Ghaffarizadeh ◽  
Paul Macklin ◽  
Kian Kani
Keyword(s):  
Breast Cancer ◽  
Genetic Algorithm ◽  
Cell Migration ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cancer Cell Migration ◽  
Computational Pipeline ◽  
Migration Assay ◽  
User Friendly

We describe an integrated experimental-computational pipeline for quantifying cell migration in vitro. This pipeline is robust to image noise, open source, and user friendly. The experimental component uses the Oris cell migration assay (Platypus Technologies) to create migration regions. The computational component of the pipeline creates masks in Matlab (MathWorks) to cell-covered regions, uses a genetic algorithm to automatically select the migration region, and outputs a metric to quantify cell migration. In this work we demonstrate the utility of our pipeline by quantifying the effects of a drug (Taxol) and of the extracellular Anterior Gradient 2 (eAGR2) protein on the migration of MDA-MB-231 cells (a breast cancer cell line). In particular, we show that inhibiting eAGR2 reduces migration of MDA-MB-231 cells.

scholarly journals Quantification of cancer cell migration with an integrated experimental-computational pipeline

2017 ◽  
Author(s):  
Edwin F. Juarez ◽  
Carolina Garri ◽  
Ahmadreza Ghaffarizadeh ◽  
Paul Macklin ◽  
Kian Kani
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cancer Cell Migration ◽  
Computational Pipeline ◽  
Migration Assay ◽  
User Friendly ◽  
Migration Of Cells

AbstractWe describe an integrated experimental-computational pipeline for quantifying cell migration in vitro. This pipeline is robust to image noise, open source, and user friendly. The experimental component uses the Oris cell migration assay (Platypus Technologies) to create migration regions. The computational component of the pipeline creates masks in Matlab (MathWorks) to cell-covered regions, uses a genetic algorithm to automatically select the migration region, and outputs a metric to quantify the migration of cells. In this work we demonstrate the utility of our pipeline by quantifying the effects of a drug (Taxol) and of the secreted Anterior Gradient 2 (sAGR2) protein in the migration of MDA-MB-231 cells (a breast cancer cell line). In particular, we show that blocking sAGR2 reduces migration of MDA-MB-231 cells.

scholarly journals Wnt5a induces ROR1 to recruit cortactin to promote breast-cancer migration and metastasis

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Md Kamrul Hasan ◽  
George F. Widhopf ◽  
Suping Zhang ◽  
Sharon M. Lam ◽  
Zhouxin Shen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Surface Protein ◽  
Cancer Cell Migration ◽  
Breast Cancer Cell Migration ◽  
Promote Breast Cancer

Abstract ROR1 is a conserved oncoembryonic surface protein expressed in breast cancer. Here we report that ROR1 associates with cortactin in primary breast-cancer cells or in MCF7 transfected to express ROR1. Wnt5a also induced ROR1-dependent tyrosine phosphorylation of cortactin (Y421), which recruited ARHGEF1 to activate RhoA and promote breast-cancer-cell migration; such effects could be inhibited by cirmtuzumab, a humanized mAb specific for ROR1. Furthermore, treatment of mice bearing breast-cancer xenograft with cirmtuzumab inhibited cortactin phosphorylation in vivo and impaired metastatic development. We established that the proline at 841 of ROR1 was required for it to recruit cortactin and ARHGEF1, activate RhoA, and enhance breast-cancer-cell migration in vitro or development of metastases in vivo. Collectively, these studies demonstrate that the interaction of ROR1 with cortactin plays an important role in breast-cancer-cell migration and metastasis.

Synthetic Macrolides that Inhibit Breast Cancer Cell Migration in Vitro

2007 ◽  
Vol 129 (9) ◽  
pp. 2434-2435 ◽  
Author(s):  
Belhu B. Metaferia ◽  
Lin Chen ◽  
Heather L. Baker ◽  
Xin-Yun Huang ◽  
Carole A. Bewley
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Migration ◽  
Breast Cancer Cell Migration

scholarly journals Breast Fibroblasts and ECM Components Modulate Breast Cancer Cell Migration through the Secretion of MMPs in a 3D Microfluidic Co-Culture Model

Cancers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1173 ◽  
Author(s):  
Karina M. Lugo-Cintrón ◽  
Max M. Gong ◽  
José M. Ayuso ◽  
Lucas A. Tomko ◽  
David J. Beebe ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Progression ◽  
Cancer Cell Migration ◽  
Migration Distance ◽  
Breast Fibroblasts

The extracellular matrix (ECM) composition greatly influences cancer progression, leading to differential invasion, migration, and metastatic potential. In breast cancer, ECM components, such as fibroblasts and ECM proteins, have the potential to alter cancer cell migration. However, the lack of in vitro migration models that can vary ECM composition limits our knowledge of how specific ECM components contribute to cancer progression. Here, a microfluidic model was used to study the effect of 3D heterogeneous ECMs (i.e., fibroblasts and different ECM protein compositions) on the migration distance of a highly invasive human breast cancer cell line, MDA-MB-231. Specifically, we show that in the presence of normal breast fibroblasts, a fibronectin-rich matrix induces more cancer cell migration. Analysis of the ECM revealed the presence of ECM tunnels. Likewise, cancer-stromal crosstalk induced an increase in the secretion of metalloproteinases (MMPs) in co-cultures. When MMPs were inhibited, migration distance decreased in all conditions except for the fibronectin-rich matrix in the co-culture with human mammary fibroblasts (HMFs). This model mimics the in vivo invasion microenvironment, allowing the examination of cancer cell migration in a relevant context. In general, this data demonstrates the capability of the model to pinpoint the contribution of different components of the tumor microenvironment (TME).

scholarly journals The effect of ethyl acetat fraction of Caesalpinia sappan L. wood on PC3 cancer cell line : cell viability and migration study

2020 ◽  
Vol 11 (2) ◽  
pp. 100-105
Author(s):  
Suyatmi Suyatmi ◽  
Endang Listyaningsih Suparyanti ◽  
Riza Novierta Pesik
Keyword(s):  
Prostate Cancer ◽  
Cell Migration ◽  
Ethyl Acetate ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Ethyl Acetate Fraction ◽  
Caesalpinia Sappan ◽  
Migration Assay

Latar Belakang: Tingginya insidensi kanker di Indonesia, termasuk kanker prostat menimbulkan beban ekonomi kesehatan yang tinggi bagi Indonesia. Pengembangan terapi kanker berbasis sumber daya alam lokal dapat membantu meringankan beban negara. Penelitian ini bertujuan untuk mengetahui  potensi aktivitas anti-kanker fraksi ethyl acetat Caesalpinia sappan L. terhadap sel line kanker PC3 yang merupakan model in vitro kanker prostat. Metode: Fraksi ethyl acetat kayu secang (Caesalpinia sappan L.) diperoleh melalui proses liquid chromatography. Efek Fraksi 9 dari Fraksi ethyl acetat kayu secang terhadap aktivitas anti-proliferasi dan migrasi sel diuji menggunakan desain uji in vitro. Hambatan proliferasi sel diukur dengan metode MTT assay, sedangkan aktivitas migrasi sel diukur dengan metode migration assay Hasil: Fraksi 9 dari Fraksi Ethyl acetat kayu secang memperlihatkan hambatan proliferasi sel line kanker PC3 dengan IC50:14.99μg/ml. Hasil migration assay menunjukkan pada dosis 10μg/ml Fraksi 9 menghambat migrasi sel line kanker PC3, sedangkan pada dosis 100μg/ml sel line kanker PC3 mati. Kesimpulan: Fraksi 9 dari fraksi Ethyl acetat kayu secang menunjukkan aktifitas anti-proliferasi dan anti-migrasi yang kuat terhadap pertumbuhan sel line kanker PC3 secara in vitro. Kata kunci : Ethyl Acetat fraction, Caesalpinia sappan, prostate cancer, PC3, migrasi sel   Abstract Background: The high incidence of cancer, including prostate cancer, in Indonesia create a high burden on health economic cost. Development of cancer therapy based on local natural resources may help the country to alleviate the burden. This research aimed to find out the potency of selected compound of Ethyl Acetate fractions of Caesalpinia sappan as anti-cancer  by using PC3 cancer cell line as an in vitro model of prostate cancer. Methods: Ethyl Acetate fraction of Caesalpinia sappan L.heartwood was prepared using a liquid chromatography method. The effect of Ethyl acetate fraction 9 on anti-proliferative and cell migration activities was assessed using MTT assay and migration assay. Results: Fraction-9 of Ethyl Acetate fraction of Caesalpinia sappan L. wood showed inhibition of PC3 cancer cell line proliferation. The IC50 of the fraction was 14.99μg/ml. The migration assay showed inhibition of cell migration on dose 10μg/ml compared to the 0 doses, while most of the cells cultured was dead when treated with 100μg/ml Fraction 9.   Conclusion: Ethyl Acetate fraction 9 of Caesalpinia sappan L heartwood possibly has anti-cancer properties based on its anti-proliferative and anti-migration activities against PC3 cancer cell line. Keywords:  Ethyl Acetate fraction, Caesalpinia sappan, prostate cancer, PC3, cell migration

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