scholarly journals Gustatory dysfunction in relation to circumvallate papilla’s taste buds structure upon unilateral maxillary molar extraction in Wistar rats: an in vivo study

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 1667
Author(s):  
Sana Mostafa ◽  
Heba M. Hakam ◽  
Amal El-motayam
Keyword(s):  
Group Iv ◽  
Taste Buds ◽  
Group Iii ◽  
Maxillary Molars ◽  
Maxillary Molar ◽  
Underlying Mechanisms ◽  
Maxillary Molar Extraction ◽  
Taste Thresholds

Background: The interaction between taste sensation and dentoalveolar innervation is still under research.  teeth loss can alter taste thresholds in humans, but the underlying mechanisms are still obscure. This study investigated the effect of unilateral maxillary molars extraction on the structure of circumvallate papilla in rats. Methods: Thirty-two male Wister rats, aged 3-4 months were randomly distributed into four groups (one control and 3 experimental ) each including 8 animals. The rats were euthanized 3, 6 or 9 weeks following the procedure. The changes in trough length and the taste buds structure and number of both sides of CVP were investigated using routine histological examination followed by statistical analysis. Results: the trough toward the extraction side was obviously shorter with a noticeable decrease of taste buds’ number than the non-extraction side. Taste buds were reduced in size and most of them showed signs of degeneration which was more evident in group II followed by group III, less deformity detected in group IV in comparison to the preceding 2 experimental groups. the non-extraction side of all experimental groups showed normal trough length and generally normal histology of taste buds.  Conclusions: Maxillary molars extraction has a degenerative effect on the structure of  taste buds and gustatory epithelium which were more marked at the extraction side and showed improvement upon elongation of follow up period

Effect of pulsatile flow on microvascular resistance in adult rabbit lungs

1992 ◽  
Vol 72 (1) ◽  
pp. 73-81 ◽  
Author(s):  
J. U. Raj ◽  
P. Kaapa ◽  
J. Anderson
Keyword(s):  
Vascular Resistance ◽  
Pulsatile Flow ◽  
Group Iv ◽  
Adult Rabbit ◽  
Total Resistance ◽  
Group Iii ◽  
Group I ◽  
Isolated Lungs ◽  
Microvascular Resistance

We have determined the effect of pulsatile flow on segmental vascular resistance in lungs from 29 adult rabbits. In group I (n = 4), II (n = 8), and III (n = 8) lungs were isolated. In group IV (n = 9) rabbits were anesthetized, their chests were opened, and lungs were studied in vivo. Group I and II lungs had steady-flow perfusion: group I with intact vasotonus and group II with papaverine treatment. Group III lungs (papaverine treated) were perfused for two consecutive 45-min periods with steady and pulsatile flow. In all isolated lungs and in lungs of five anesthetized rabbits, we measured pressures in subpleural 20- to 50-microns-diam arterioles and venules by use of the micropipette servo-nulling method. Measurement of distribution of blood flow in lungs of four anesthetized rabbits by use of radiolabeled microspheres revealed no abnormality of blood flow to the micropunctured lobe. We found that total and segmental vascular resistances were similar in group I and II lungs, with microvessels representing 55% of total resistance. In group III lungs, total resistance was 30% lower during pulsatile flow than during steady flow because of a lower microvascular resistance. Lungs in vivo (group IV) had a significantly lower total vascular resistance than isolated lungs and had a low fractional resistance in microvessels (approximately 28%). We conclude that, in isolated perfused adult rabbit lungs, vascular resistance is very high, particularly in the microvascular segment, and that pulsatile flow decreases microvascular resistance.

scholarly journals Treatment of maxillary molar with furcation involvement - Case report

2019 ◽  
Vol 1 (1) ◽  
pp. 22-27
Keyword(s):  
Case Report ◽  
Survival Rates ◽  
Clinical Report ◽  
Class Iii ◽  
Maxillary Molars ◽  
Maxillary Molar ◽  
Furcation Involvement ◽  
Similar Survival ◽  
Oral Hygiene Instructions

Purposes: The aim of this clinical report is to discuss the advantages and the steps of the root separation and resection approach in a maxillary molar with a class III furcation of the palatine root with a follow-up of 24 months. Case report: A patient with tooth mobility and bleeding after probing, was diagnosed with a Chronic Periodontitis Generalized Moderate and Localized Severe. Hygienic phase with oral hygiene instructions, scaling and root planning was performed. Due to class III furcation of the palatine root of the teeth 16 and some remaining pockets deepths, was decided to execute an apical repositioning flap on the 1st sextant among with the palatine root amputation combined with the endodontic treatment. During the surgical procedure, it was also performed a reduction of the palate thickness from teeth 15 to 17; in distal area of the 17 a distal wedged was performed in order to remove all the pockets. After the surgery, the patient was inserted in a long-lasting individualized supportive periodontal treatment.This case has a follow up of 24 months. Conclusions: Similar survival rates are described in the literature when we compared the root amputation with the dental implants survival rate, but the first one is cheaper and allows to keep the natural tooth. We can’t forget that the periodontitis is a risk factor of peri-implantitis. This clinical case shows that, with a high motivated patient and with the complete periodontal treatment, is possible to obtain excellent results with root separation and resection in maxillary molars class III.

scholarly journals In Vivo Measurement of Conduction Velocities in Afferent and Efferent Nerve Fibre Groups in Mice

2012 ◽  
pp. 203-214 ◽  
Author(s):  
H. STEFFENS ◽  
P. DIBAJ ◽  
E. D. SCHOMBURG
Keyword(s):  
Sural Nerve ◽  
Common Peroneal Nerve ◽  
Group Iv ◽  
Efferent Nerve ◽  
Group Iii ◽  
In Vivo Measurement ◽  
Adult Mice ◽  
Group I ◽  
The Common

Electrophysiological investigations in mice, particularly with altered myelination, require reference data of the nerve conduction velocity (CV). CVs of different fibre groups were determined in the hindlimb of anaesthetized adult mice. Differentiation between afferent and efferent fibres was performed by recording at dorsal roots and stimulating at ventral roots, respectively. Correspondingly, recording or stimulation was performed at peripheral hindlimb nerves. Stimulation was performed with graded strength to differentiate between fibre groups. CVs of the same fibre groups were different in different nerves of the hindlimb. CVs for motor fibres were for the tibial nerve (Tib) 38.5±4.0 m/s (Aγ: 16.7±3.0 m/s), the sural nerve (Sur) 39.3±3.1 m/s (12.0±0.8 m/s) and the common peroneal nerve (Per) 46.7±4.7 m/s (22.2±4.4 m/s). CVs for group I afferents were 47.4±3.1 m/s (Tib), 43.8±3.8 m/s (Sur), 55.2±6.1 m/s (Per) and 42.9±4.3 m/s for the posterior biceps (PB). CVs of higher threshold afferents, presumably muscle and cutaneous, cover a broad range and do not really exhibit nerve specific differences. Ranges are for group II 22-38 m/s, for group III 9-19 m/s, and for group IV 0.8-0.9 m/s. Incontrovertible evidence was found for the presence of motor fibres in the sural nerve. The results are useful as references for further electrophysiological investigations particularly in genetically modified mice with myelination changes.

scholarly journals Inflammatory stimuli acutely modulate peripheral taste function

2016 ◽  
Vol 115 (6) ◽  
pp. 2964-2975 ◽  
Author(s):  
Devaki Kumarhia ◽  
Lianying He ◽  
Lynnette Phillips McCluskey
Keyword(s):  
Taste Buds ◽  
Taste Perception ◽  
Transport Systems ◽  
Salt Consumption ◽  
Tnf Α ◽  
Inflammatory Stimuli ◽  
Trpv1 Antagonist ◽  
Underlying Mechanisms ◽  
Acute Changes

Inflammation-mediated changes in taste perception can affect health outcomes in patients, but little is known about the underlying mechanisms. In the present work, we hypothesized that proinflammatory cytokines directly modulate Na+ transport in taste buds. To test this, we measured acute changes in Na+ flux in polarized fungiform taste buds loaded with a Na+ indicator dye. IL-1β elicited an amiloride-sensitive increase in Na+ transport in taste buds. In contrast, TNF-α dramatically and reversibly decreased Na+ flux in polarized taste buds via amiloride-sensitive and amiloride-insensitive Na+ transport systems. The speed and partial amiloride sensitivity of these changes in Na+ flux indicate that IL-1β and TNF-α modulate epithelial Na+ channel (ENaC) function. A portion of the TNF-mediated decrease in Na+ flux is also blocked by the TRPV1 antagonist capsazepine, although TNF-α further reduced Na+ transport independently of both amiloride and capsazepine. We also assessed taste function in vivo in a model of infection and inflammation that elevates these and additional cytokines. In rats administered systemic lipopolysaccharide (LPS), CT responses to Na+ were significantly elevated between 1 and 2 h after LPS treatment. Low, normally preferred concentrations of NaCl and sodium acetate elicited high response magnitudes. Consistent with this outcome, codelivery of IL-1β and TNF-α enhanced Na+ flux in polarized taste buds. These results demonstrate that inflammation elicits swift changes in Na+ taste function, which may limit salt consumption during illness.

scholarly journals Signal-averaged electrocardiogram in chronic Chagas' heart disease

1995 ◽  
Vol 113 (2) ◽  
pp. 851-857 ◽  
Author(s):  
Aguinaldo Pereira de Moraes ◽  
Paulo Jorge Moffa ◽  
Eduardo A. Sosa ◽  
Giovanni M. V. Bellotti ◽  
Carlos A. Pastore ◽  
...  
Keyword(s):  
Heart Disease ◽  
Time Domain Analysis ◽  
Group Iv ◽  
Root Mean Square Amplitude ◽  
Group Iii ◽  
Group I ◽  
Chagas Heart Disease ◽  
Group Ii ◽  
The Relationship

The aim of the study was to register the prevalence of late potentials (LP) in patients with chronic Chagas' heart disease (CCD) and the relationship with sustained ventricular tachycardia (SVT). 192 patients (96 males), mean age 42.9 years, with CCD were studied through a Signal Averaged ECG using time domain analysis. According to presence or absence of bundle branch block (BBB) and SVT, four groups of patients were created: Group I (n = 72): without SVT (VT-) and without BBB (BBB-): Group II (n = 27): with SVT (VT+) and BBB-; Group III (n = 63): VT- and with BBB (BBB+); and Group IV (N = 30): VT+ and BBB+. The LP was admitted, with 40 Hz filter, in the groups without BBB using standard criteria of the method. In the group with BBB, the root-mean-square amplitude of the last 40 ms (RMS) < =14µV was considered as an indicator of LP. RESULTS: In groups I and II, LP was present in 21 (78%) of the patients with SVT and in 22 (31%) of the patients without SVT (p < 0.001), with Sensitivity (S) 78%; Specificity (SP) 70% and Accuracy (Ac) 72%. LP was present in 30 (48%) of the patients without and 20 (67%) of the patients with SVT, in groups III and IV. p = 0.066, with S = 66%; SP = 52%; and Ac = 57%. In the follow-up, there were 4 deaths unrelated to arrhythmic events, all of them did not have LP. Eight (29,6%) of the patients from group II and 4 (13%) from group IV presented recurrence of SVT and 91,6% of these patients had LP. CONCLUSIONS: LP occurred in 77.7% of the patients with SVT and without BBB. In the groups with BBB, there was association of LP with SVT in 66,6% of the cases. The recurrence of SVT was present in 21% of the cases from which 91,6% had LP.

Parasympathomimetic Effect of Shilajit Accounts for Relaxation of Rat Corpus Cavernosum

2012 ◽  
Vol 7 (2) ◽  
pp. 119-127 ◽  
Author(s):  
Sarabjeet Kaur ◽  
Pravin Kumar ◽  
Deo Kumar ◽  
M. D. Kharya ◽  
Nityanand Singh
Keyword(s):  
Corpus Cavernosum ◽  
Saline Group ◽  
Group Iv ◽  
In Vivo Study ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Vitro Effect

Previous studies have reported an enhancement of central cholinergic signal cascade by shilajit. For the present study, it was hypothesized that parasympathomimetic effect of shilajit accounting for relaxation of rat corpus cavernosum may be one of the major mechanisms attributing to its traditional role as an aphrodisiac. To test this hypothesis, the acute peripheral effect of standard acetylcholine (ACh), shilajit, and their combination was evaluated on cardiorespiratory parameters such as mean arterial blood pressure (MABP), heart rate (HR), respiratory rate (RR), and neuromuscular transmission (NMT). Furthermore, in vitro effect of standard ACh, shilajit, and their combination was tested on the rat corpus cavernosum. Six groups were used for the in vivo study ( N = 5): Group I (control-saline), Group II (ACh), Group III (Sh), Group IV (Sh followed by ACh), Group V (Atropine followed by ACh), and Group VI (Atropine followed by Sh). The in vitro study included four groups: Group I (control-saline), Group II (ACh), Group III (Sh), and Group IV (Sh followed by ACh). The results of the in vivo study confirmed the peripheral parasympathomimetic effect of shilajit (400 µg/mL). The in vitro results revealed that shilajit (400 and 800 µg/mL) relaxed cavernous strips’ concentration dependently and enhanced ACh-mediated relaxations. The peripheral parasympathomimetic effects of shilajit were confirmed by blockade of shilajit-induced relaxations (in vitro) and shilajit-induced lowering of MABP and HR (in vivo) by atropine.

scholarly journals Analysis of the length of fixation during hemivertebra resection. A monocentric cohort

2020 ◽  
Author(s):  
Sergey O Ryabykh ◽  
Egor Yu Filatov ◽  
Joseph Olorunsogo Mejabi ◽  
Dmitry M Savin ◽  
Alexander B Gubin
Keyword(s):  
Blood Loss ◽  
Cobb Angle ◽  
Spinal Deformity ◽  
Operating Time ◽  
Group Iv ◽  
Group Iii ◽  
Fixation Group ◽  
Group I ◽  
Segmental Fixation

Abstract Background: We aimed at reviewing results of surgical correction of spinal deformity due to hemivertebra with regard to length of instrumentation. Methods: Study comprised 117 patients with congenital spinal deformity treated between 2010 and 2018. Patients’ aged 1 to 18 years. Mean follow-up was 3 years (1 to 8). Posterior approach was used in all cases. Patients were subdivided into 4 groups. Result: Unilateral monosegmental fixation (Group I) for 15 children with mean age of 48.3 months. Cobb angle for scoliosis and kyphosis were 31.1 0 + 6.4 0 and 29.3° + 11.9 0 corrected to 7.8 0 + 6.8 0 and 4.7 0 + 4.0 0 respectively. Blood loss was 213.6 mL. Operating time was 165 minutes. Bilateral monosegmental fixation (Group II) for 24 patients with mean age of 53.1 months. Cobb angle for scoliosis and kyphosis were 32.5° + 8.6 0 and 30.9° + 4.3 0 corrected to 5.3 0 + 2.8 0 and 0.2 0 + 11.6 0 respectively. Blood loss was 215.4 mL. Operating time was 160.5 minutes. Bilateral three-segmental fixation (Group III) for 29 patients with mean age of 78.2 months. Cobb angle for scoliosis and kyphosis were 36.6° + 10.6 0 and 37.6° + 14.6 0 corrected to 6.2 0 + 6.1 0 and 5.0 0 + 5.2 0 respectively. Blood loss was 342.7 mL. Operating time was 197.0 minutes. Bilateral polysegmental fixation (Group IV) for 49 children with mean age of 112.7 months. Cobb angle for scoliosis and kyphosis were 40.2° + 14.8 0 and 58.2° + 35.6 0 corrected to 10.7 0 + 9.6 0 and 10.7 0 + 10.3 0 respectively. Blood loss was 549.3 mL. Operating time was 288.8 minutes. Conclusion: Choice of spinal fixation technique and the length of fixation were determined on the basis of patient’s age, magnitude of the deformity and concomitant vertebral abnormalities.

scholarly journals Analysis of the length of fixation during hemivertebra resection. A monocentric retrospective cohort

2020 ◽  
Author(s):  
Sergey O Ryabykh ◽  
Egor Yu Filatov ◽  
Joseph Olorunsogo Mejabi ◽  
Dmitry M Savin ◽  
Alexander B Gubin
Keyword(s):  
Blood Loss ◽  
Cobb Angle ◽  
Spinal Deformity ◽  
Operating Time ◽  
Group Iv ◽  
Group Iii ◽  
Fixation Group ◽  
Group I ◽  
Segmental Fixation

Abstract Background: We aimed at reviewing results of surgical correction of spinal deformity due to hemivertebra with regard to the age, severity of deformity and length of instrumentation.Methods. Study comprised 117 patients with congenital spinal deformity treated between 2010 and 2018. Patients’ aged 1 to 18 years. Mean follow-up was 3 years (1 to 8). Posterior approach was used in all cases. Patients were subdivided into 4 groups.Result:Unilateral mono-segmental fixation (Group I) for 15 children with mean age of 48.3 months. Cobb angle for scoliosis and kyphosis were 31.10 + 6.40 and 29.3° + 11.90 corrected to 7.80 + 6.80 and 4.70 + 4.00 respectively. Blood loss was 213.6 mL. Operating time was 165 minutes.Bilateral mono-segmental fixation (Group II) for 24 patients with mean age of 53.1 months. Cobb angle for scoliosis and kyphosis were 32.5° + 8.60 and 30.9° + 4.30 corrected to 5.30 + 2.80 and 0.20 + 11.60 respectively. Blood loss was 215.4 mL. Operating time was 160.5 minutes.Bilateral three-segmental fixation (Group III) for 29 patients with mean age of 78.2 months. Cobb angle for scoliosis and kyphosis were 36.6° + 10.60 and 37.6° + 14.60 corrected to 6.20 + 6.10 and 5.00 + 5.20 respectively. Blood loss was 342.7 mL. Operating time was 197.0 minutes.Bilateral poly-segmental fixation (Group IV) for 49 children with mean age of 112.7 months. Cobb angle for scoliosis and kyphosis were 40.2° + 14.80 and 58.2° + 35.60 corrected to 10.70 + 9.60 and 10.70 + 10.30 respectively. Blood loss was 549.3 mL. Operating time was 288.8 minutes.Conclusion: Choice of spinal fixation technique and the length of fixation were determined on the basis of patient’s age, magnitude of the deformity and concomitant vertebral abnormalities.

Clinical Follow-up of a Fissure Sealant Placed Using Different Adhesive Protocols: A 24-month Split-mouth Study

2018 ◽  
Vol 43 (4) ◽  
pp. 362-371 ◽  
Author(s):  
AR Yazici ◽  
E Ozturk Bayazit ◽  
ZB Kutuk ◽  
G Ozgunaltay ◽  
E Ergin ◽  
...  
Keyword(s):  
Retention Rate ◽  
Group Iv ◽  
Retention Rates ◽  
Fissure Sealant ◽  
Group Iii ◽  
Group I ◽  
Etch And Rinse ◽  
Acid Etch ◽  
Self Etch

SUMMARY The purpose of this study was to evaluate the retention rates of a fissure sealant placed using different adhesive protocols over 24 months. Twenty-four subjects with no restorations or caries received fissure sealants (Clinpro Sealant, 3M ESPE) placed using different adhesive protocols. A total of 292 sealants were placed as follows by two previously calibrated dentists using a table of random numbers (n=73): group I, acid-etch/without adhesive; group II, with a self-etch adhesive (Adper Easy Bond, 3M ESPE); group III, with an etch-and-rinse adhesive (Adper Single Bond 2, 3M ESPE); group IV, with acid + self-etch adhesive (Adper Easy Bond). Two other calibrated examiners independently evaluated the sealants at baseline and at six-, 12-, 18-, and 24-month recalls. Each sealant was evaluated in terms of caries formation being present or absent and retention using the following criteria: 1 = total retention, 2 = partial loss, and 3 = total loss. Pearson's χ2 test was used to evaluate differences in retention rates among the sealants for each evaluation period. At the end of 24 months, total retention rates were 57.5%, 27.4%, 84.9%, and 76.7% in the acid-etch, self-etch adhesive, etch-and-rinse adhesive, and acid + self-etch adhesive groups, respectively. Although there were no statistically significant differences between the retention rates among the adhesive protocols at 6 months (p=0.684), significant differences were observed at the 12-, 18-, and 24-month evaluations. At 24 months, the lowest retention rates were observed in the self-etch group (p&lt;0.05). No caries development was observed in any of the groups. The retention rate of sealants placed using self-etch adhesive was poor compared with the other groups.

scholarly journals Comparison of Surgical Outcomes with Time Interval of Operation among extradural haematoma (EDH) Patients

2018 ◽  
Vol 4 (1) ◽  
pp. 33-39
Author(s):  
MM Ehsanul Haque ◽  
Shamima Sultana ◽  
Md Saiful Alam ◽  
Wahida Begum ◽  
Syeda Asfiya Ara
Keyword(s):  
Surgical Intervention ◽  
Group Iv ◽  
Time Interval ◽  
Extradural Haematoma ◽  
Group Iii ◽  
Medical College ◽  
Group I ◽  
Study Population ◽  
Death Cases

Background: Post-operative outcome of extradural haematoma (EDH) patients depends on the time interval of operation.Objectives: The purpose of the present study was to evaluate the role of time interval and surgical intervention in EDH.Methodology: This interventional study was carried out in the Department of Neurosurgery at Dhaka Medical College and Hospital, Dhaka, Bangladesh from July 2001 to July 2003 for a period of two (02) years. Patients with the history of head trauma admitted in Neurosurgery unit of Dhaka Medical College and Hospital which were being diagnosed as EDH both clinically and radiologically were included as study population. All patients were treated surgically and haematoma was evacuated. Outcome regarding neurological status, functional recovery, associated morbidity and mortality were assessed in each case as per Glasgow Outcome Scale and was compared between the two groups of patients who were treated surgically within 3rd day and 4th to 7th day after infliction of injury. The study population was devided into 4 groups on the basis of the consciousness level on admission of the patients. The EDH patietns who had GCS <5, 5 to 8, 9 to 12 and 13 to 15 were categorized as group I, II, III and IV respectively. On the basis of pre operative time interval, surgical intervention was done within 3 days of injury and from 4th to 7th day of injury. Craniotomy and craniectomy were done depending on patient’s condition and situation of haematoma.Result: A total 63 patients were included. During admission out of 63 patients majority of the patients were in the group III which was 29(46%) cases. During pre-operqative period out of 63 patients majority of the patients were in the group III which was 30(47.6%) cases. Out of 63 cases a total number of 40 cases were performed the surgery within 72 hours and the rest 23 patients were from 4th day to 7th day of injury. In group I at 8th POD, 3 death cases were recorded at 3rd day operation group and 2 cases at 4th to 7th cases. In group IV at 8th POD follow up good recovery was reported in 6 cases at 3rd day and 3 cases at 4th to 7th day. In group I after 1 month, 3 death cases were recorded at 3rd day operation group and 2 cases at 4th to 7th cases. In group IV after 1 month follow up good recovery was reported in 6 cases at 3rd day and 3 cases at 4th to 7th day; however, moderate disability was reported in 2 cases at 4th to 7th day.Conclusion: In conclusion mortality rate is reduced in patients with EDH who are treated in the earliest possible time after head injuryJournal of National Institute of Neurosciences Bangladesh, 2018;4(1): 33-39

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