scholarly journals Atopic biomarker changes after exposure to Porphyromonas gingivalis lipopolysaccharide: a small experimental study in Wistar rats

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 371
Author(s):  
Sindy Cornelia Nelwan ◽  
Ricardo Adrian Nugraha ◽  
Anang Endaryanto ◽  
Asti Meizarini ◽  
Udijanto Tedjosasongko ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Wistar Rats ◽  
Periodontal Pathogen ◽  
Rapid Decline ◽  
Group 4 ◽  
Area Of Interest ◽  
Before And After ◽  
Porphyromonas Gingivalis Lipopolysaccharide ◽  
Group 3 ◽  
Group 2

Background: IgE and IgG4 are implicated in atopic development and clinically utilized as major biomarkers. Atopic responses following certain pathogens, such as Porphyromonas gingivalis (Pg), are currently an area of interest for further research. The aim of this study is to measure the level of IgE, IgG4, and IgG4/IgE ratio periodically after exposure of periodontal pathogen Pg lipopolysaccharide (LPS). Methods: We used 16 Wistar rats (Rattus norvegicus) randomly subdivided into four groups: Group 1,  injected with placebo; Group 2, injected with LPS Pg 0.3 µg/mL; Group 3, injected with LPS Pg 1 µg/mL; and Group 4, injected with LPS Pg 3 µg/mL. Sera from all groups were taken from retro-orbital plexus before and after exposure. Results: Levels of IgE and IgG4 increased significantly following exposure of LPS Pg at day-4 and day-11. Greater increase of IgE rather than IgG4 contributed to rapid decline of IgG4/IgE ratio, detected in the peripheral blood at day-4 and day-11. Conclusion: Modulation of atopic responses following exposure to LPS Pg is reflected by a decrease in IgG4/IgE ratio that accompanies an increase of IgE. Therefore, Pg, a keystone pathogen during periodontal disease, may have a tendency to disrupt atopic biomarkers.

scholarly journals Atopic Biomarker Changes after Exposure to Porphyromonas gingivalis Lipopolysaccharide: A Small Experimental Study in Wistar Rat

2021 ◽  
Author(s):  
Sindy Cornelia Nelwan ◽  
Ricardo Adrian Nugraha ◽  
Anang Endaryanto ◽  
Asti Meizarini ◽  
Udijanto Tedjosasongko ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Wistar Rat ◽  
Periodontal Pathogen ◽  
Rapid Decline ◽  
Group 4 ◽  
Area Of Interest ◽  
Before And After ◽  
Porphyromonas Gingivalis Lipopolysaccharide ◽  
Group 3 ◽  
Group 2

BackgroundIgE and IgG4 are implicated in atopic development and clinically utilized as major biomarkers. Atopic responses following certain pathogens such as Porphyromonas gingivalis is currently an area of interest for further research.PurposeThe aim of this study is to measure the level of IgE, IgG4, and IgG4/IgE ratio periodically after exposure of periodontal pathogen Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS).MethodsWe used 16 wistar rats (Rattus norvegicus) randomly subdivided into 4 groups, group 1 were injected by placebo, group 2 by LPS Pg 0.3 μg/mL,group 3 by LPS Pg 1 μg/mL, and group 4 by LPS Pg 3 μg/mL. Sera from both groups were taken from retro-orbital plexus before and after exposure.ResultsLevel of IgE and IgG4 increased significantly following exposure of LPS Pg at day-4 and day-11. Greater increase of IgE rather than IgG4 contributes to rapid decline of IgG4/IgE ratio, detected in the peripheral blood at day-4 and day-11.ConclusionModulation of atopic responses following exposure to LPS Pg is reflected by decrease in IgG4/IgE ratio that accompanies an increase of IgE.Clinical significancePorphyromonas gingivalis, a keystone pathogen during periodontal disease, may have a tendency to disrupt atopic biomarkers.

scholarly journals The Effect of Broccoli Sprout Extract on Seasonal Grass Pollen-Induced Allergic Rhinitis

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1337
Author(s):  
Joseph Yusin ◽  
Vivian Wang ◽  
Susanne M. Henning ◽  
Jieping Yang ◽  
Chi-Hong Tseng ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Spray ◽  
Placebo Tablet ◽  
Group 4 ◽  
Cytokine Levels ◽  
Before And After ◽  
Group 3 ◽  
Nasal Steroid ◽  
Group 2 ◽  
Broccoli Sprout

Patients exposed to pollutants are more likely to suffer from allergic rhinitis and may benefit from antioxidant treatment. Our study determined if patients diagnosed with grass-induced allergic rhinitis could benefit from broccoli sprout extract (BSE) supplementation. In total, 47 patients were confirmed with grass-induced allergic rhinitis and randomized to one of four groups: group 1 (nasal steroid spray + BSE), group 2 (nasal steroid spray + placebo tablet), group 3 (saline nasal spray + BSE) and group 4 (saline nasal spray + placebo tablet). Peak Nasal Inspiratory Flow (PNIF), Total Nasal Symptoms Scores (TNSS) and nasal mucus cytokine levels were analyzed in samples collected before and after the 3-week intervention. Comparing before and after the intervention, PNIF improved significantly when comparing Groups 1 and 2, vs. placebo, at various time points (p ≤ 0.05 at 5, 15, 60 and 240 min) following nasal challenge, while TNSS was only statistically significant at 5 (p = 0.03), 15 (p = 0.057) and 30 (p = 0.05) minutes. There were no statistically significant differences in various cytokine markers before and after the intervention. Combining nasal corticosteroid with BSE led to the most significant improvement in objective measures.

scholarly journals Effect of Solanum gilo leaf extract on some haematological indices of albino Wistar rats

2021 ◽  
Vol 12 (1) ◽  
pp. 108-111
Author(s):  
Nsimah Monday Essien ◽  
Justina Nwadimma Nwangwa ◽  
Charles Cyprian Mfem ◽  
Johnbull Martins Uket ◽  
Efiok Aniekan Archibong
Keyword(s):  
Blood Cell ◽  
Wistar Rats ◽  
Blood Parameters ◽  
Diet Group ◽  
Control Group ◽  
Carbohydrate Consumption ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Haematological Indices

Solanum gilo (eggplant) is an edible vegetable whose leaf is reported to possess numerous medicinal properties due to its rich constituents. Against this backdrop, there is possibility that its consumption may have effect on haematological indices. Therefore this research sought to assess the effect of Solanum gilo leaf diet on some haemotological indices of albino wistar rats. About twenty four (24) albino wistar rats weighing between 120-150mg were randomly assigned into four groups of six animals each. Each rat was allowed to drinking water ad labitum. Control group received normal rodent chow while group 2 was fed 5% Solanum gilo diet. Group 3 was fed 10% Solanum gilo diet and group 4 received 20% Solanum gilo diet. The different diets were prepared by mixing different proportions of ground S. gilo leaves with rodent chow. Automated blood cell analyzer (Model PCE 210, Japan) was used for analysis of haematological parameters. Preliminary study on its proximate composition, showed the appreciable presence of crude fat, protein, fibre and carbohydrate. Consumption of S. gilo caused significant improvement in some blood parameters; notably packed cell volume (PCV), white blood cell (WBC) and platelet counts. Hence, eggplant is a nutritious vegetable with healing, the therapeutic and immunity properties. If exploited in human diet has the potentials of improving quality of life.

scholarly journals Evaluation of protective effect of myricetin, a bioflavonoid in dimethyl benzanthracene-induced breast cancer in female Wistar rats

2014 ◽  
Vol 03 (02) ◽  
pp. 107-111 ◽  
Author(s):  
J. K. Jayakumar ◽  
P. Nirmala ◽  
B.A. Praveen Kumar ◽  
Ashok P. Kumar
Keyword(s):  
Breast Cancer ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Treated Animal ◽  
Group 4 ◽  
Female Wistar Rats ◽  
Group 3 ◽  
Group 2 ◽  
And Control

Abstract Background: Breast cancer is one of the most common cancers worldwide. Alarmingly, the incidence of breast cancer is rising rapidly in India. Aim: The present research was focused to assess the role of myricetin; a bioflavonoid in 7,12-dimethylbenzanthracene (DMBA)-induced breast cancer in female Wistar rats. Materials and Methods: A total of 36 female Wistar rats (total 6 groups, n = 6 per group) 6 - 8 weeks old, weighing 150 gm were used in the study. DMBA was given at the dose of 7.5 mg/kg subcutaneously in the mammary region once a week for 4 consecutive weeks in group 2. Vincristine was given in the dose of 500 μg/kg intraperitonially every week for 4 consecutive weeks in group 3. Myricetin was given orally in a dose of 50, 100, and 200 mg/kg in group 4, 5, and 6 respectively. The statistical significance of the data was determined using one way analysis of variance and Duncan’s multiple range test. Results: The result showed that myricetin increased the antioxidant levels in plasma, erythrocyte lysate, and breast tissue and was effective in preventing the oxidative damage induced by the carcinogen DMBA. Myricetin 50, 100, and 200 mg/kg/oral for 120 days treated animal resulted comparable results to that of standard vincristine and control groups. Conclusions: Myricetin was found to be either equieffective or more effective than vincristine in all the parameters studied. Myricetin proved the capacity of flavonols to act as antioxidant in cells represents a potential treatment in the field of oncology.

scholarly journals The effects of local nitroglycerin on the surgical delay procedure in prefabricated flaps by vascular implant in rats

2012 ◽  
Vol 27 (12) ◽  
pp. 905-911 ◽  
Author(s):  
Jairo Zacchê de Sá ◽  
José Lamartine de Andrade Aguiar ◽  
Adriana Ferreira Cruz ◽  
Alexandre Ricardo Pereira Schuler ◽  
José Ricardo Alves de Lima ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Delay Procedure ◽  
Surgical Delay ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
The Mean ◽  
Group 2 ◽  
Prefabricated Flap ◽  
Group 1

PURPOSE: To evaluate the effect of local nitroglycerin on the viable area of a prefabricated flap for vascular implant in rats, and to investigate the surgical delay procedure. METHODS: A femoral pedicle was implanted under the skin of the abdominal wall in forty Wistar rats. The animals were divided into four groups of ten: group 1 - without surgical delay procedure and local nitroglycerin; group 2 - with surgical delay procedure, but without local nitroglycerin; group 3 - without surgical delay procedure, but with local nitroglycerin; and group 4 - with simultaneous surgical delay procedure and local nitroglycerin. The percentages of the viable areas, in relation to the total flap, were calculated using AutoCAD R 14. RESULTS: The mean percentage value of the viable area was 8.9% in the group 1. 49.4% in the group 2; 8.4% in the group 3 and 1.1% in the group 4. There was significant difference between groups 1 and 2 (p=0.005), 1 and 4 (p=0.024), 2 and 3 (p=0.003), 2 and 4 (p=0.001). These results support the hypothesis that the closure of the arterial venous channels is responsible for the phenomenon of surgical delay procedure. CONCLUSION: Local nitroglycerin did not cause an increase in the prefabricated viable flap area by vascular implantation and decreased the viable flap area that underwent delay procedures.

scholarly journals Preventing intraperitoneal adhesions with vitamin E and sodium hyaluronate/carboxymethylcellulose: a comparative study in rats

2008 ◽  
Vol 23 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Fredy Corrales ◽  
Marcelo Corrales ◽  
Carlos Cauduro Schirmer
Keyword(s):  
Vitamin E ◽  
Wistar Rats ◽  
Adhesion Formation ◽  
Sodium Hyaluronate ◽  
Postoperative Adhesions ◽  
Intraperitoneal Adhesions ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

PURPOSE: To compare the effectiveness of intraperitoneally administered vitamin E with the sodium hyaluronate/carboxymethylcellulose membrane (HA/CBMC) in preventing postoperative intraperitoneal adhesion formation. METHODS: Sixty Wistar rats underwent a laparotomy and adhesions were induced (IA). The animals were divided into four groups: group 1, control (IA); group 2 (IA + Vitamin E): group 3 (IA+HA/CBMC) and group 4 (IA+ Vitamin E + HA/CBMC). The Vitamin E (groups 2 and 4) and HA/CBMC (groups 3 and 4) were administered intraperitoneally before the abdominal wall was closed. After 30 days, adhesions were classified by an independent surgeon. RESULTS: Three animals died; one from group 3 and two from group 4. All control animals had substantial adhesions compared with unsubstantial adhesions observed in 11/15 in group 2 (P = 0.000), 11/14 in group 3 (P = 0.001), and 10/13 in group 4 (P = 0.000). CONCLUSION: Vitamin E, administered intraperitoneally, is as effective as HA/CBMC in preventing postoperative adhesions.

scholarly journals Effect of Three Wavelengths of Diode Laser on the Efficacy of Bleaching of Stained Teeth

2020 ◽  
Author(s):  
Reza Saeedi ◽  
Ladan Ranjbar Omrani ◽  
Mahdi Abbasi ◽  
Nasim Chiniforush ◽  
Mojgan Kargar
Keyword(s):  
Diode Laser ◽  
Tooth Bleaching ◽  
Color Parameters ◽  
Group 4 ◽  
Before And After ◽  
Group 3 ◽  
Group 2 ◽  
Laser Bleaching ◽  
Group 1

Objectives: Light irradiation and heat have been used to accelerate the process of tooth bleaching. This study aimed to assess the efficacy of conventional bleaching compared to laser-bleaching using three different wavelengths of diode lasers. Materials and Methods: In this in-vitro experimental study, 40 extracted human central incisors were immersed in a coloring solution made of tea, coffee, and cola for 21 days. The L*, a*, and b* color parameters were measured before and after the immersion using spectrophotometry. The teeth were then randomly divided into four groups (n=10) as follows: group 1: 810-nm diode laser + Biolase Laser White 20, group 2: 940-nm diode laser + Biolase Laser White 20, group 3: 980-nm diode laser + Biolase Laser White 20, and group 4: conventional bleaching with Opalescence Boost without laser irradiation. One-way analysis of variance (ANOVA) was used to assess the effect of laser type and bleaching technique on color parameters. Results: The 940-nm (∆E=28.5896) and 810-nm laser groups (∆E=21.2382) showed the highest and the lowest bleaching efficacy, respectively; however, the groups were not significantly different in terms of bleaching efficacy (P>0.05). Conclusion: Laser-bleaching with 810-, 940- and 980-nm wavelengths of diode laser has an efficacy similar to that of conventional bleaching but in a shorter period. No difference was noted between different laser wavelengths in terms of bleaching efficacy.

scholarly journals Preliminary Study on the Effect of Methanolic Extract of Watermelon (Citrullus lanatus) Rind on Prednisolone Suppressed Immunity in Male Wistar Rats

2021 ◽  
pp. 1-6
Author(s):  
Nyejirime Young Wike ◽  
Mobisson Samuel Kelechi ◽  
Godspower Onyeso ◽  
Okekem Amadi ◽  
Elizabeth Eepho Krukru
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Citrullus Lanatus ◽  
Control Group ◽  
Group 4 ◽  
Watermelon Rind ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

Citrullus lanatus thumb (Cucurbitaceae) commonly called watermelon is widely consumed in this part of the world as food and medicine. This study was carried out to examine the effect of methanolic extract of watermelon (Citrullus lanatus) rind on prednisolone suppressed immunity in male wistar rats. A total of 20 male wistar rats weighing 150-294g were used in 4 groups with five rats each. Group 1, the control group was given distilled water and feed, Group 2 was given 200 mg/kg body weight of methanolic extract of watermelon rind, Group 3 rats were given 2.5 mg/kg body weight of prednisolone and Group 4 rats were given 2.5 mg/kg body weight of prednisolone and 200 mg/kg body weight of methanolic extract of watermelon rind. Prednisolone and the methanolic extract of watermelon rind were administered orally for a period of 30 days. Blood samples were collected by cardio puncture from the rats for white blood cell (WBC), lymphocyte, and granulocyte and monocyte counts at the end of the experiment.  The data were statistically analysed using one-way ANOVA (Analysis of variance). Data were considered significant at p<0.05. The results obtained showed that methanolic extract of watermelon rind caused a significant increasen in immune function of rats when compared with the control and immune suppressed rats.

scholarly journals Effects of Methanolic Root Extract of Holarrhena floribunda on the Lipid Profile and Sex Hormones in Wistar Rats

2019 ◽  
pp. 1-7
Author(s):  
Dennis, Amaechi ◽  
E. O. Udosen ◽  
V. S. Ekam ◽  
I. P. Ekpe
Keyword(s):  
Lipid Profile ◽  
Sex Hormones ◽  
Wistar Rats ◽  
Test Group ◽  
Control Group ◽  
Root Extract ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim: This study aims to determine the effects of methanolic root extract of Holarrehna floribunda on the level of some serum sex hormones, follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, estradiol, progesterone and lipid profile in Wistar rats.   Materials and Methods: Twenty- four male and female Wistar rats (150-250 g body weight) were randomly assigned into 4 groups of 6 rats each. Group 1 (control male) took normal rat chow and drinking water. Group 2 (control female) took normal rat chow and drinking water, Group 3(Male test group), was administered with 200 mg/kg of Holarrehna floribunda extract, Group 4(Female test group), was administered with 200 mg/kg of Holarrehna floribunda. The feeding regimens lasted for 5weeks. Results: The values for the lipid profile shows CHOL in Group 1 and Group 3 were 4.02±0.41 mmol/l  and 5.75±0.09 mmol/l, Group 2 and Group 4 were 3.87±0.22 mmol/l  and 5.80±0.10 mmol/l  respectively, TRIG  for Group 1 and Group 3 were 1.30±0.05 mmol/l  and 2.11±0.15 mmol/l  for Groups 2 and 4 were 1.15±0.03 mmol/l  and (2.10±0.12 mmol/l,HDL-C for Groups 1 and 3 were 30.01±0.82 mg/dL  and 39.88± 1.24mg/dL,for Groups 2 and 4 were 29.98± 0.77 mg/dL  and 38.20± 1.83 mg/dL, LDL-C for Groups 1 and 3 were 2.26±0.37 mmol/L  and 4.17±0.35 mmol/L respectively, for Groups 2 and 4 were 3.27±0.47 mmol/L  and 4.06±0.21 mmol/L  respectively, shows significant increase(p<0.05) in HDL-C (mg/dL) in Groups 1,2,3 and 4.The values obtained showed significant increase (p<0.05) in HDL-C (mg/dL) in Groups 1,2,3and 4The serum concentrations of FSH, Estradiol, LH and progesterone in the control Group 2 were 0.38 ±0.07mIU/mL, 7.83±0.35 mIU/mL, 15.50±0.15 Pg/mL and 0.86±0.03 ng/mL respectively, and in test Group 4 were 0.77±0.04mIU/mL, 8.75±0.17 mIU/mL, 21.09±0.79 Pg/Ml 0.33±0.05 ng/mL. Follicle stimulating hormone levels was significantly higher (p<0.05) in test group compared with control. While in Group 1 and 3, the serum concentrations of FSH, LH, and testosterone in control Group 1 were (0.51 ±0.06) mIU/mL, (16.44±0.31) mIU/mL and (8.41±0.50) ng/mL respectively and in test Group 3 were 0.88±0.06 mIU/mL19.88±1.46mIU/mL and10.68±0.64 ng/mL, respectively. Conclusion: The extract improves the level of sex hormones in both the male and female rats.  which could enhance reproductive functions in normal rats and those with loss of reproductive function.

scholarly journals Anti-inflammation effects of Sardinella longicep oil against paw oedema on Rattus novergicus induced by 1% carrageenan

2010 ◽  
Vol 43 (3) ◽  
pp. 113
Author(s):  
Rima Parwati Sari ◽  
Yenny Sugiharto
Keyword(s):  
Wistar Rats ◽  
Term Therapy ◽  
Group 4 ◽  
Normality Test ◽  
Paw Oedema ◽  
Group 3 ◽  
Sardinella Longiceps ◽  
Group 2 ◽  
Long Term Therapy ◽  
Anti Inflammation

Background: People usually used non steroid anti-inflammation drugs (NSAID) such as aspirin in chronic inflammation treatment. However, using NSAID at long term therapy will cause many effects such as nausea and vomiting. Sardinella longiceps oil, on the other side, is reported as an alternative treatment for anti-inflammation since it is natural and also contained eicosapentaenoid acid (EPA) and decohexaenoic acid (DHA). Thus, it may reduce paw oedema. Purpose: The aim of this study was to know anti-inflammation effects of Sardinella longiceps oil against paw oedema of Wistar rats induced by 1% carrageenan. Methods: The samples of this research were 32 Wistar rats which were divided into four groups, in group 1, the rats were given aquadest; in group 2, the rats were given aspirin; in group 3, the rats were given 1 ml Sardinella longiceps oil; in group 4, the rats were given 1.5 ml Sardinella longiceps oil. All of the rats, nevertheless, were given intraplantar induction of 1% carrageenan into the paw of rats to induce the inflammation condition. Results: All data were tested with normality test. The normal data were then analyzed with Homogenity of Variances and also ANOVA test which result showed significant differences. The data which showed significant differences were tested again with LSD test. result then showed that group given 1 ml Sardinella longiceps oil and group given 1.5 ml Sardinella longiceps oil had no significant differences from group given aspirin, but there were significant differences between group given 1 ml Sardinella longiceps oil and group given 1.5 ml Sardinella longiceps oil, and also between group given 1.5 ml Sardinella longiceps oil and group given aquadest. Conclusion: Sardinella longiceps oil could reduce paw oedema in Wistar rats induced with 1% carrageenan.Latar belakang: Penggunaan obat anti-inflamasi non steroid (OAINS) seperti aspirin sering digunakan dalam pengobatan inflamasi kronis. Namun penggunaan OAINS dalam jangka waktu panjang akan menyebabkan efek samping seperti mual dan muntah. Di sisi lain, minyak Sardinella longiceps, dilaporkan dapat digunakan sebagai alternatif anti-inflamasi karena selain alami, juga terdapat kandungan eicosapentaenoid acid (EPA) and decohexaenoic acid (DHA) yang dapat mengurangi edema pada telapak kaki. Tujuan: Tujuan dari penelitian ini adalah mengetahui efek anti-inflamasi minyak Sardinella longiceps pada edema telapak kaki tikus Wistar yang diinduksi karagenan 1%. Metode: Sampel penelitian ini adalah 32 tikus yang dibagi ke dalam empat kelompok. Kelompok 1, tikus diberi akuades; kelompok 2, tikus diberi aspirin; kelompok 3, tikus diberi minyak Sardinella longiceps 1 ml; kelompok 4, tikus diberi minyak Sardinella longiceps 1,5 ml. Semua tikus tidak terkecuali, diinduksi intraplantar karagenan 1% secara intraplantar pada telapak kaki untuk membuat kondisi inflamasi. Hasil: Semua data dilakukan uji normalitas. Selanjutnya data yang berdistribusi normal dilakukan uji homogenitas dan juga uji ANOVA yanng hasilnya menunjukkan adanya perbedaan bermakna. Data yang menunjukkan perbedaan bermakna diuji lagi dengan LSD. Pada uji LSD menunjukkan bahwa tidak ada perbedaan bermakna antara kelompok minyak Sardinella longiceps 1 ml dengan kelompok aspirin dan kelompok minyak Sardinella longiceps 1,5 ml dengan kelompok aspirin, tetapi ada perbedaan bermakna antara kelompok minyak Sardinella longiceps 1 ml dan kelompok akuades dengan kelompok minyak Sardinella longiceps 1.5 ml group given aquadest. Kesimpulan: Sardinella longiceps dapat mengurangi edema pada telapak kaki tikus Wistar yang diinduksi karagenan 1%.

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