scholarly journals Unusual Survival Kinetics of Neocarzinostatin-treated HeLa Cells: Its Relation to the Drug-inactivation.

1991 ◽  
Vol 32 (2) ◽  
pp. 191-201 ◽  
Author(s):  
YOKO KURODA ◽  
TAKEHITO SASAKI ◽  
FUMIHIKO HOSHINO
Keyword(s):  
Hela Cells ◽  
Kinetics Of

Kinetics of the respiratory syncytial virus growth cycle in Hela cells

1969 ◽  
Vol 28 (2) ◽  
pp. 122-132 ◽  
Author(s):  
S. Levine ◽  
R. Hamilton
Keyword(s):  
Respiratory Syncytial Virus ◽  
Hela Cells ◽  
Growth Cycle ◽  
Virus Growth ◽  
Syncytial Virus ◽  
Kinetics Of

A stress-inducible 40 kDa protein (hsp40): purification by modified two-dimensional gel electrophoresis and co-localization with hsc70(p73) in heat-shocked HeLa cells

1993 ◽  
Vol 104 (3) ◽  
pp. 629-638 ◽  
Author(s):  
H. Hattori ◽  
T. Kaneda ◽  
B. Lokeshwar ◽  
A. Laszlo ◽  
K. Ohtsuka
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Hela Cells ◽  
Gel Electrophoresis ◽  
Recovery Period ◽  
Chinese Hamster ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Terminal Amino ◽  
Kinetics Of

We have previously reported that a novel 40 kDa protein is induced by heat shock and several environmental stresses in mammalian and avian cells and that the N-terminal amino acid sequence of this 40 kDa protein has homology with the bacterial DnaJ heat-shock protein. We have purified this protein (40 kDa heat-shock protein, hsp40) from HeLa cells by modified two-dimensional gel electrophoresis and generated a polyclonal antibody against hsp40. This antibody was highly specific for human hsp40 and cross-reacted weakly with rat and Chinese hamster hsp40. Indirect immunofluorescence revealed that the hsp40 in HeLa cells accumulates in the nucleus, especially in the nucleolus, during heat shock and returns to the cytoplasm during the recovery period. The kinetics of the accumulation in the nucleoli and subsequent return to the cytoplasm of hsp40 was similar to that of hsp70. In addition, hsp40 was co-localized with hsc70(p73) in heat-shocked HeLa cells as demonstrated by double immunofluorescence staining. These results suggest that hsp40 (a DnaJ homologue) and hsp70 (a DnaK homologue) may act in concert to repair (refold) denatured proteins and protein aggregates in the nuclei and nucleoli of heat-shocked HeLa cells.

scholarly journals Heterogeneity and Kinetics of FRET-Based Ca2+ and Zn2+ Sensors in HeLa Cells

2012 ◽  
Vol 102 (3) ◽  
pp. 209a
Author(s):  
Hairong Ma ◽  
Emily A. Gibson ◽  
Philip J. Dittmer ◽  
Kevin Dean ◽  
Amy E. Palmer ◽  
...  
Keyword(s):  
Hela Cells ◽  
Kinetics Of

KINETICS OF A DRUG SENSITIVE CLONE OF HeLa CELLS

1970 ◽  
Vol 3 (2) ◽  
pp. 207-215
Author(s):  
A. H. W. Nias ◽  
Margaret Fox ◽  
B. W. Fox
Keyword(s):  
Hela Cells ◽  
Sensitive Clone ◽  
Kinetics Of

Phosphorescence Kinetics of Singlet Oxygen in HeLa Cells Suspension

2019 ◽  
Vol 45 (9) ◽  
pp. 936-939
Author(s):  
V. P. Belik ◽  
D. M. Beltukova ◽  
T. N. Belyaeva ◽  
I. M. Gadzhiev ◽  
E. S. Kornilova ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Hela Cells ◽  
Kinetics Of

Cytotoxic Activity of Cu/TiO2 Nanoparticles on Uterine-Cervical Cancer Cells

2020 ◽  
Vol 20 (12) ◽  
pp. 7289-7298
Author(s):  
Samuel González-García ◽  
Aída Hamdan-Partida ◽  
Emma Elisa Ortiz Islas ◽  
Miguel Ángel Zavala Sánchez ◽  
Julia Pêrez Ramos ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cells ◽  
Copper Complexes ◽  
Sol Gel ◽  
Uterine Cervical Cancer ◽  
Degraded Dna ◽  
Scanning Calorimetry ◽  
Biological Interest ◽  
Cervical Cancer Cells ◽  
Kinetics Of

Nanoparticles based on metal oxides serve as carrier matrices for molecules of biological interest. In this work, we used different copper complexes that were coupled to TiO2 nanoparticles. Nanoparticles were prepared with the sol–gel method. The Cu/TiO2 nanoparticles were characterized through ultraviolet-visible and Fourier transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, nitrogen physisorption analysis, and scanning electron microscopy. Their biological activity was determined through DNA degradation and their cytotoxic effect on HeLa cells. The Cu/TiO2 nanoparticles presented a pore size between 2 and 6 nm, the size of nanoparticles agglomerates was between 100 and 500 nm. The nanoparticles of Cu/TiO2 degraded DNA starting at 15 min. The half maximal inhibitory concentration in HeLa cells depends on the used cooper complexes, the kinetics of cell death is of first order. Results revealed that these nanoparticles could be applied in uterine-cervical cancer treatment.

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