Characterization of Probiotic Properties ofEnterococcus faeciumNHRD IHARA Isolated from Porcine Feces

2013 ◽  
Vol 77 (9) ◽  
pp. 1967-1969 ◽  
Author(s):  
Shengbin RAO ◽  
Yasuhiro IHARA ◽  
Shin SUKEGAWA ◽  
Fumihiro ARAKAWA ◽  
Tatsuya FUJIMURA ◽  
...  
Keyword(s):  
Probiotic Properties ◽  
Porcine Feces

scholarly journals Characterization of a Lactobacillus brevis strain with potential oral probiotic properties

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Fang Fang ◽  
Jie Xu ◽  
Qiaoyu Li ◽  
Xiaoxuan Xia ◽  
Guocheng Du
Keyword(s):  
Lactobacillus Brevis ◽  
Probiotic Properties

In vitro characterization of probiotic properties ofPediococcus pentosaceus BH105 isolated from human faeces

2009 ◽  
Vol 59 (3) ◽  
pp. 485-491 ◽  
Author(s):  
Başar Uymaz ◽  
Ömer Şimşek ◽  
Nefise Akkoç ◽  
Haluk Ataoğlu ◽  
Mustafa Akçelik
Keyword(s):  
Probiotic Properties ◽  
Human Faeces ◽  
In Vitro Characterization

scholarly journals Intraspecific Genotypic Characterization of Lactobacillus rhamnosus Strains Intended for Probiotic Use and Isolates of Human Origin

2006 ◽  
Vol 72 (8) ◽  
pp. 5376-5383 ◽  
Author(s):  
M. Vancanneyt ◽  
G. Huys ◽  
K. Lefebvre ◽  
V. Vankerckhoven ◽  
H. Goossens ◽  
...  
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Starter Cultures ◽  
Probiotic Properties ◽  
Human Origin ◽  
Culture Collections ◽  
Clinical Case Studies ◽  
Probiotic Strains ◽  
Different Sources

ABSTRACT A set of 118 strains of the species Lactobacillus rhamnosus was collected, including probiotic strains, research strains with potential probiotic properties, food starter cultures, and human isolates. The majority of the strains were collected from companies, hospitals, or culture collections or were obtained after contacting authors who reported clinical case studies in the literature. The present work aimed to reveal the genotypic relationships between strains of these diverse sources. All strains were initially investigated using fluorescent amplified fragment length polymorphism (FAFLP) with three different primer combinations. Numerical analysis of FAFLP data allowed (i) confirmation of the identification of all strains as members of L. rhamnosus and (ii) delineation of seven stable intraspecific FAFLP clusters. Most of these clusters contained both (potentially) probiotic strains and isolates of human origin. For each of the clusters, strains of different sources were selected for pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments obtained with the enzymes NotI and AscI. Analysis of PFGE data indicated that (i) some (potentially) probiotic strains were indistinguishable from other probiotic strains, suggesting that several companies may use duplicate cultures of the same probiotic strain, and (ii) in a number of cases human isolates from sterile body sites were indistinguishable from a particular probiotic strain, suggesting that some of these isolates may be reisolations of commercial strains.

Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain

2012 ◽  
Vol 30 (1) ◽  
pp. 59-67 ◽  
Author(s):  
R. Cebrián ◽  
A. Baños ◽  
E. Valdivia ◽  
R. Pérez-Pulido ◽  
M. Martínez-Bueno ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Probiotic Properties ◽  
Functional Safety ◽  
Producer Strain

Characterization of lactic acid bacteria isolated from a Greek dry-fermented sausage in respect of their technological and probiotic properties

Meat Science ◽  
2003 ◽  
Vol 65 (2) ◽  
pp. 859-867 ◽  
Author(s):  
E. Papamanoli ◽  
N. Tzanetakis ◽  
E. Litopoulou-Tzanetaki ◽  
P. Kotzekidou
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Probiotic Properties ◽  
Fermented Sausage ◽  
Dry Fermented Sausage

Some probiotic properties of chicken lactobacilli

1999 ◽  
Vol 45 (12) ◽  
pp. 981-987 ◽  
Author(s):  
Carlos Gusils ◽  
Silvia N González ◽  
G Oliver
Keyword(s):  
Probiotic Properties ◽  
Epithelial Surface ◽  
Adhesion Ability ◽  
Bivalent Cations ◽  
Calcium Requirement ◽  
Salmonella Pullorum ◽  
Vitro Model ◽  
Lactobacillus Animalis

The beneficial effect of lactobacilli has been attributed to their ability to colonize human and animal gastrointestinal tracts. In this work, adhesion assays with three lactobacillus strains and intestinal fragments obtained from chickens were assessed. Lactobacillus animalis and L. fermentum were able to adhere to three kinds of epithelial cells (crop, small and large intestines) with predominance to small intestine. Among the strains considered, L. fermentum subsp. cellobiosus showed the lowest and L. animalis the highest adhesion ability. Scanning electron microphotographs showing L. animalis and L. fermentum adhering to intestinal cells were obtained. The characterization of L. animalis adhesion indicated that lectin-like structure of this strain has glucose/mannose as specific sugars of binding. However, a calcium requirement was not observed. The adhesion of L. fermentum was reduced by addition of sialic acid or mannose (P < 0.01). These carbohydrates can be involved in the interaction between adhesin and epithelial surface. In this case, the dependence on bivalent cations was demonstrated. Lactobacillus fermentum was effective in reducing the attachment of Salmonella pullorum by 77%, while L. animalis was able to inhibit (90%, 88%, and 78%) the adhesion of S. pullorum, S. enteritidis, and S. gallinarum to host-specific epithelial fragments respectively. Our results from this in vitro model suggest that these lactobacilli are able to block the binding sites for Salmonella adhesion.Key words: lactobacilli, lectin-like structures, poultry adhesion, probiotic properties.

scholarly journals Characterization of Enterococcus faecium CCDM 922 in Respect of its Technological and Probiotic Properties

2016 ◽  
Vol 5 (5) ◽  
pp. 474-482 ◽  
Author(s):  
Ivana Hyrslova ◽  
Gabriela Krausova ◽  
Jirina Bartova ◽  
Libor Kolesar ◽  
Zoran Jaglic ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Probiotic Properties

scholarly journals PROBIOTIC CHARACTERIZATION OF BACILLUS SUBTILIS STRAIN ISOLATED FROM INFANT FECAL MATTER REVEALED BY 16S rRNA GENE AND PHYLOGENETIC ANALYSIS

2021 ◽  
pp. 77-85
Author(s):  
DEVARANJAN DAS ◽  
CHANDI CHARAN RATH ◽  
NAKULANANDA MOHANTY ◽  
SMITA HASINI PANDA
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Bile Salt ◽  
Rrna Gene ◽  
Probiotic Properties ◽  
Fecal Matter ◽  
Antimicrobial Metabolites

Objective: The rationale of our study was to isolate and identify the putative probiotic strain from infant fecal matter exhibiting a broad range of antimicrobial activity and to analyze the effect of different culturing conditions on its probiotic properties and the production of antimicrobial metabolites. Methods: In the present study, bacterial strains were screened for probiotic properties and antimicrobial activity from infant fecal matter (6 months–2 years). The effect of varying culture conditions such as tolerance to acid, bile salt, phenol, NaCl, pH, incubation period, and temperature along with autoaggregation assay, hydrophobicity, and hemolysis was studied. The characterization of the potent strain was studied by morphological, biochemical, and 16S rRNA gene sequencing along the phylogenetic affiliation of the strain was studied. Results: Two putative probiotic bacteria (DAM and IFM) were isolated, identified, characterized, and predicted at pH 2.0, 3.0, and 4.0, the isolate IFM had 50%, 60%, and 70% survivability, while isolate DAM had 55%, 63%, and 75% survivability, respectively. At a bile salt concentration of 0.5%, both isolates had a 75% survival rate. The isolates exhibited a high percentage of hydrophobicity and autoaggregation. The isolates also had non-hemolytic activity and were susceptible to many clinical tested antibiotics (tetracycline, erythromycin, ampicillin, gentamycin, penicillin, etc.). The isolate showed antimicrobial activity against enteric pathogens such as Staphylococcus aureus, Escherichia coli, and Shigella dysenteriae. The accession number of Bacillus subtilis MT279753 and MK453362 was submitted to NCBI. Conclusion: The result revealed that isolates have potent probiotic properties and possess a direct influence on the production of antimicrobial metabolites. These parameters can be modified for the improvement of the potentiality of the isolates.

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