Protoplast Formation from Schizophyllum commune by a Culture Filtrate of Bacillus circulans KA-304 Grown on a Cell-wall Preparation of S. commune as a Carbon Source

1997 ◽  
Vol 61 (5) ◽  
pp. 852-857 ◽  
Author(s):  
Katsushige Mizunq ◽  
Osamu Kimura ◽  
Takashi Tachik
Keyword(s):  
Cell Wall ◽  
Carbon Source ◽  
Culture Filtrate ◽  
Schizophyllum Commune ◽  
Bacillus Circulans ◽  
Protoplast Formation ◽  
Cell Wall Preparation ◽  
A Cell

scholarly journals The Effect of Carbon Dioxide on Fruiting and on the Degradation of a Cell-wall Glucan in Schizophyllum commune

1977 ◽  
Vol 102 (2) ◽  
pp. 385-389 ◽  
Author(s):  
J. H. SIETSMA ◽  
D. RAST ◽  
J. G. H. WESSELS
Keyword(s):  
Carbon Dioxide ◽  
Cell Wall ◽  
Schizophyllum Commune ◽  
A Cell

A cell wall preparation of Enterococcus faecalis strain EC-12 stimulates ?-defensin expression in newly hatched broiler chicks

2007 ◽  
Vol 78 (1) ◽  
pp. 92-97 ◽  
Author(s):  
Yuri SAKAI ◽  
Takamitsu TSUKAHARA ◽  
Noritaka MATSUBARA ◽  
Kazunari USHIDA
Keyword(s):  
Cell Wall ◽  
Enterococcus Faecalis ◽  
Broiler Chicks ◽  
Cell Wall Preparation ◽  
A Cell

scholarly journals Incorporation of Magnetic Nanoparticles into Protoplasts of Microalgae Haematococcus pluvialis: A Tool for Biotechnological Applications

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5068
Author(s):  
Maria G. Savvidou ◽  
Angelo Ferraro ◽  
Evangelos Hristoforou ◽  
Diomi Mamma ◽  
Dimitris Kekos ◽  
...  
Keyword(s):  
Cell Wall ◽  
Magnetic Nanoparticles ◽  
Mechanical Treatment ◽  
Genetic Manipulation ◽  
Haematococcus Pluvialis ◽  
Biotechnological Applications ◽  
Protoplast Formation ◽  
Cell Recovery ◽  
Microalgae Biomass ◽  
A Cell

Intensive research on the use of magnetic nanoparticles for biotechnological applications of microalgae biomass guided the development of proper treatment to successfully incorporate them into these single-cell microorganisms. Protoplasts, as cells lacking a cell wall, are extensively used in plant/microalgae genetic manipulation as well as various biotechnological applications. In this work, a detailed study on the formation of protoplasts from Haematococcus pluvialis with the use of enzymatic and mechanical procedures was performed. The optimization of several parameters affecting the formation of protoplasmic cells and cell recovery was investigated. In the enzymatic treatment, a solution of cellulase was studied at different time points of incubation, whereas in the mechanical treatment, glass beads vortexing was used. Mechanical treatment gave better results in comparison to the enzymatic one. Concerning the cell recovery, after the protoplast formation, it was found to be similar in both methods used; cell viability was not investigated. To enhance the protoplast cell wall reconstruction, different “recovery media” with an organic source of carbon or nitrogen were used. Cell morphology during all treatments was evaluated by electron microscopy. The optimal conditions found for protoplast formation and cell reconstruction were successfully used to produce Haematococcus pluvialis cells with magnetic properties.

scholarly journals Analysis of Antitumor Properties of Effector Cells Stimulated with a Cell Wall Preparation (WPG) of Bifidobacterium infantis.

1995 ◽  
Vol 18 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Kazunori SEKINE ◽  
Jun OHTA ◽  
Masatoshi ONISHI ◽  
Taro TATSUKI ◽  
Yukiko SHIMOKAWA ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bifidobacterium Infantis ◽  
Effector Cells ◽  
Cell Wall Preparation ◽  
A Cell ◽  
Antitumor Properties

Tumor-Suppressive Effect of a Cell Wall Preparation, WPG, from Bifidobacterium infantis in Germfree and Flora-Bearing Mice

1991 ◽  
Vol 10 (1) ◽  
pp. 43-52 ◽  
Author(s):  
Shigeo TSUYUKI ◽  
Shoji YAMAZAKI ◽  
Hideyasu AKASHIBA ◽  
Hiroshi KAMIMURA ◽  
Kazunori SEKINE ◽  
...  
Keyword(s):  
Cell Wall ◽  
Suppressive Effect ◽  
Bifidobacterium Infantis ◽  
Cell Wall Preparation ◽  
A Cell ◽  
Tumor Suppressive Effect

CELLOBIOSE AS A PARAMORPHOGEN IN SCHIZOPHYLLUM COMMUNE

1967 ◽  
Vol 13 (12) ◽  
pp. 1663-1670 ◽  
Author(s):  
Ronald W. Wilson ◽  
Donald J. Niederpruem
Keyword(s):  
Cell Wall ◽  
Carbon Source ◽  
Mycelial Growth ◽  
Sole Carbon Source ◽  
Schizophyllum Commune ◽  
Cell Wall Fraction ◽  
Soluble Cell ◽  
Activity Of Enzymes ◽  
Increased Activity

The mycelial growth of Schizophyllum commune on cellobiose, as sole carbon source, produced colonies that were quite dense and restricted in diameter. The hyphae were composed of shorter, more highly branched cells when grown on this disaccharide. Cellobiose brought about an increase in the ratio of an alkali-soluble cell-wall fraction (S-glucan) to an alkali-insoluble cell-wall fraction (R-glucan). This change might be explained by increased activity of enzymes which hydrolyze the R-glucan component following growth in cellobiose. Thus, a system is described which attempts to relate wall-softening enzymes to morphology.

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