Phylogenetic Analysis and Heterologous Expression of Surface Layer Protein SlpC ofBacillus sphaericusC3-41

Spirillum serpens possesses a surface layer protein which exhibits a regular hexagonal packing of the morphological subunits. A morphological model of the structure of the protein has been proposed at a resolution of about 25 Å, in which the morphological unit might be described as having the appearance of a flared-out, hollow cylinder with six ÅspokesÅ at the flared end. In order to understand the detailed association of the macromolecules, it is necessary to do a high resolution structural analysis. Large, single layered arrays of the surface layer protein have been obtained for this purpose by means of extensive heating in high CaCl2, a procedure derived from that of Buckmire and Murray. Low dose, low temperature electron microscopy has been applied to the large arrays.As a first step, the samples were negatively stained with neutralized phosphotungstic acid, and the specimens were imaged at 40,000 magnification by use of a high resolution cold stage on a JE0L 100B. Low dose images were recorded with exposures of 7-9 electrons/Å2. The micrographs obtained (Fig. 1) were examined by use of optical diffraction (Fig. 2) to tell what areas were especially well ordered.

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Active immunization of hamsters againstClostridium difficileinfection using surface-layer protein

FEMS Immunology & Medical Microbiology ◽

10.1111/j.1574-695x.2007.00363.x ◽

2008 ◽

Vol 52 (2) ◽

pp. 207-218 ◽

Cited By ~ 41

Author(s):

Déirdre B. Ní Eidhin ◽

Julie B. O'Brien ◽

Matthew S. McCabe ◽

Verónica Athié-Morales ◽

Dermot P. Kelleher

Keyword(s):

Surface Layer ◽

Active Immunization ◽

Surface Layer Protein

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In-situ production of silver nanobiocomposite using surface layer protein of Lactobacillus helveticus and aqueous extract of dried Juglans regia green husk and investigation of antibacterial activity

Polymer Bulletin ◽

10.1007/s00289-021-03895-4 ◽

2021 ◽

Author(s):

Fatemeh Rahimzadeh ◽

Parinaz Ghadam ◽

Rouha Kasra-Kermanshahi ◽

Mahboobeh Zarrabi

Keyword(s):

Antibacterial Activity ◽

Surface Layer ◽

Aqueous Extract ◽

Juglans Regia ◽

Lactobacillus Helveticus ◽

Surface Layer Protein ◽

In Situ Production

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A Computational Approach for Protein-Protein Interactions of Bacterial Surface Layer Protein with Human Erb3 and αIIB-β3 Receptors

Biointerface Research in Applied Chemistry ◽

10.33263/briac121.420430 ◽

2021 ◽

Vol 12 (1) ◽

pp. 420-430

Keyword(s):

Surface Layer ◽

Molecular Docking ◽

Protein Interactions ◽

Protein A ◽

Computational Approach ◽

Microbial Interactions ◽

Protein Protein Interactions ◽

Epithelial Surface ◽

Surface Receptors ◽

Surface Layer Protein

Host microbial interactions had significant factors in maintains homeostasis and immune-related activity. One such interaction made by Lactobacillus sp. with Surface layer proteins (Slps) had been studied through a computational approach. Erb3 and αIIB-β3, which are epithelial surface layer receptors, are subjected to interact with the Slp homology model. Both cell surface receptors were subjected to interact through computational docking, followed by molecular dynamics simulations through the coarse-grain method to explore the conformational stability. Through the implementation of the molecular docking for the surface layer protein A, we have shown the surface layer protein A, protein-protein interactions are higher in cellular receptors with epidermal growth factor receptor at an -34.45 ΔG and -51.19 ΔG through molecular docking with Erb3 and αIIB-β3. This study shows the unique interaction of Slp with the epithelial surface receptors like Erb3 and αIIB-β3, which are multipurpose applications in microbial-based drug therapeutics.

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