scholarly journals Immunohistochemical features of the structure of fetuses’ uterus with a gestational term of 21-28 weeks from mothers, whose pregnancy is complicated by chronic infection of the lower genital tract

2021 ◽  
Vol 11 (2) ◽  
pp. 230-245
Author(s):  
V. O. Polyasnyi ◽  
L. S. Kupriianova
Keyword(s):  
Connective Tissue ◽  
Genital Tract ◽  
Chronic Infection ◽  
Medical Science ◽  
Uterine Wall ◽  
Main Group ◽  
Immunohistochemical Method ◽  
Endocrine Disorders ◽  
Lower Genital Tract ◽  
Complicated Pregnancy

The relevance of the following article is prescribed by the growing number of perinatal mortality and morbidity among newborns due to the mother’s chronic infection of the lower genital tract (HILGT). It is commonly known, that HILGT contributes to the delay of the fetal development likewise is often manifested by the violation of internal organs and systems implementation. One of the most acute topic is the the fetus’ reproductive system in ther early gestation terms. However, the immunohistochemical features of the fetuses’ uterus structure with the gestational term of 21-28 weeks from mothers with HILGT on the current stage of the medical science develpment have not been described yet. The aim of the current research is to identify immunohistochemical features of the fetuses’ uterus structure with the term of gestation of 21-28 weeks from mothers, whose pregnancy is complicated by HILGT. Material of the research: 25 fetuses from mothers, whose course of pregnancy was physiological (group of comparison) as well as 25 fetuses from mothers, whose pregnancy was complicated by HILGT (main group). All fetuses had died intranatally, as a result of acute uterine placental and placental-fetal circulation. Research methods: organometric, histological, immunohistochemical, morphometric, statistical. The research results and their discussion: the organometric parameters of the fetuses’ uterus from the main group were clearily decreased in comparison to such parameters in case of the group of comparison. The decrease of the parameter of the uterine wall thickness of fetuses from mothers with the complicated pregnancy prescribes thinning of the main structural components. Namely: endometrium, myometrium and perimetrium. In case of endometrium of fetuses’ uterus a decrease of number of glands and the lack of their functional activity was noticed. It characterizes this layer as structurally immature.At the same time, in case of fetal organs of fetuses from mothers with complicated pregnancies, we could notice an increase in the number of apoptotically altered cells. Comparing to the structure of layers of uterine walls in case of fetuses from mothers with physiological pregnancy, in the uterus of fetuses from mothers with complicated pregnancy our attention is attracted by a massive growth of the connective tissue. In the myometrium it contributes to the disruption of the muscle fibers strusture likewise to its absence in some areas. By the immunohistochemical method it was possible to reveal a prevalence of the collagen of the III type in the structure of connective tissue likewise in the walls of blood vessels. It is connected primarily with disruption of the collagen’ maturation. The vascular component of the uterine wall in case of fetuses from mothers with HILGT differs from ones in case of fetuses from mothers with physiological pregnancy. This difference is grounded on the increase of endothelin-producing activity by vessels of both arterial and venous types. By processing preparations by monoclonal antibodies to estrogen, it has been postulated, that the intensity of the reaction as well as number of cells in the fetuses’ organs of the main group are clearily decreased comparing to ones in cases of fetuses from the group of comparison. The disorders of the fetuses’ uterus in case of fetuses from mothers with HILGT, that were provided in the current article, are prescribed by chronic hypoxia, endocrine disorders as well as influences of the infectious agents, that are taking place in case of such pathology. The characteristic features of the fetuses’ uterus structure in case of fetuses from mothers, whose pregnancy is complicated by HILGT, indicate gross violation in the organ’s implementation on the early stages of the fetal organogenesis, likewise they could be a leading link of pathogenesis of primary infertility’ development in the future ontogenesis.

scholarly journals Immunohistochemical features of the collagen’ formation in the uterus of fetuses with a gestational term of 21-28 weeks from mothers, whose pregnancy was complicated by preeclampsia of different stage of severity

2021 ◽  
Vol 11 (9) ◽  
pp. 828-840
Author(s):  
L. Kupriianova ◽  
A. Pakhlivanzade
Keyword(s):  
Connective Tissue ◽  
Study Groups ◽  
Uterine Wall ◽  
Immunohistochemical Method ◽  
Wall Structure ◽  
Endocrine Disorders ◽  
Endometrial Polyps ◽  
Collagen Formation ◽  
Severe Stage ◽  
Complicated Pregnancy

In the following article we are going to provide the results of research on uterus’ structure in case of fetuses with a gestational term of 21-28 weeks from mothers, whose pregnancy was complicated by PE of different severity stage (15 cases) comparing to one in case of fetuses from mothers with a physiological pregnancy (15 cases). All fetuses have died intranatally as a result of an acute disorder in uterine-placental and uterine-fetal circulation. The research methods: macroscopic, organometric, histological, immunohistochemical, morphometric and statictical. By applying an organometric method we have revealed a probable decrease of indexes of weight, length and thickness of the uterine’ wall in case of fetuses from mothers with PE comparing to ones in case of fetuses from healthy mothers. The observative histological research had not revealed any significant changes in the strucutre of the organs’ wall in case of fetuses from the study groups. Thus, all organs were represented by mucous, muscular and serous membranes with a clear boundary between them. The comparative morphometric research had revealed the following features of the uterus’ wall structure in case of fetuses from mothers with PE. The indexes of thickness of all structural components of the organ’s wall were clearly decreased comparing to ones in case of fetuses from mothers with a physiological pregnancy. In the uterine endometrium of fetuses from mothers with PE of moderate stage of severity the features of proliferative (hormonal) activity are taking place, however, in the meanwhile, in case of organs of fetuses from mothers with PE of severe stage of severity we could notice a probable decrease of glands’ number as well as a lack of a proliferative activity in them (hypoplastic changes). By applying immunohistochemical method with using MCAT to CD 95 we have disclosed a probable increase of an apoptotic index in organs of fetuses from mothers with a complicated pregnancy towards one in case of fetuses from healthy mothers. Among the specific features of the uterine myometrium strucutre in case of fetuses from mothers with PE we could name the following ones: decrease of the vascular component percentage as well as increase growth of the connective tissue. Moreover, the structure of the connective tissue is represented mostly by the collagen of the III type, while, in case of organs of fetuses from healthy mothers the collagen of the I type prevails. In walls of the vessels of arterial type in case of uterus of fetuses from mothers with PE we could notice an increased glow of the collagen of the III type as well as a probable decrease of glow in case of collagen of the IV type. However, in case of organs’ vessels of fetuses from healthy mothers we could notice an increse of glow of the collagen of the IV type. There is a fact, which attracts an attention to it. Namely, all aforementioned changes in the uterine wall in case of fetuses from mothers with PE are minimally manifested, when the mild course of disease took place, and was maximally manifested in case of severe one. All changes in the uterus in case of fetuses from mothers with PE of different stage of severity, that were postulated above, could contribute to formation of glanduar hyperplasia, endometrial polyps, precancerous diseases as well as endometrial cancer in the subsequent ontogenesis, as well as it could lead to impaired pregnancy and infertility. All aforementioned changes in organs of fetuses from mothers, whose pregnancy was complicated by PE of different stage of severity, were connected with changes in the vascular bed of the mother-placenta-fetus system as well as with endocrine disorders, that take place in case of this pathology, in the organism of pregnant woman.

scholarly journals Immunuhistochemical features of the ovaries’ structure in case of fetuses with a gestational term of 21-28 weeks from mother, whose pregnancy was complicated by chronic infections of lower genital tracts

2021 ◽  
Vol 11 (5) ◽  
pp. 246-261
Author(s):  
V. O. Poliyasnyi ◽  
L. S. Kupriianova
Keyword(s):  
Connective Tissue ◽  
Main Group ◽  
Relative Volume ◽  
Immunohistochemical Method ◽  
Chronic Infections ◽  
Functional Changes ◽  
Massive Growth ◽  
Cortical Substance ◽  
Complicated Pregnancy ◽  
Clear Increase

The following article presents results of the complex research on postulation of immunohistochemical features of the ovaries’ structure in case of fetuses with a gestational term of 21-28 weeks from mothers with HILGT comparing to ones in case of fetuses from mothers with a physiological pregnancy. The materials of the research are: 25 fetuses from mothers with HILGT as well as 25 fetuses from mothers with physiological pregnancy. All fetuses had died antenatally and inranatally, as a result of violation of uteroplacental and umbilical-uterine circulation. Research methods: macroscopic, organometric, histological, immunohistochemical, morphometric, statistical. The organometric method revealed a clear decrease of main indexes of the fetuses’ ovaries from mothers with a complicated pregnancy comparing to ones in case of organs of fetuses from healthy mothers. Morphometric method revealed a clear decrease of a relative volume of cortical substance as well as a clear increase of a relative volume of brain substance in the gonads of fetuses from the main group; as well as it showed a clear decrease of an index of follicular tissue’ relative volume, likewise a clear increase of an index of interstitial component relative volume.The staining by picrofuxin by van Gieson method revealed a massive growth of the connective tissue in all strucutral components of organs of fetuses from mothers with HILGT relatively to gonads of fetuses from healthy mothers. Using of MCAT to main types of collagens allowed to reveal a violation of maturation of main types of collagens in the structure of connective tissue of fetal gonads in the main group. In the follicular component of the fetuses’ ovaries in the main group are appearing mature, cystic-artretic and growing forms of follicles. Immunohistochemical method revealed an increased number of an apoptotically altered eggs in the ovaries of fetuses from mothers with HILGT comparing to one in organs of fetuses from mothers with physiological pregnancy.  The peroxidase method revealed an increased endotheline-producing activity of the vascular component as arterial, like a venous type; likewise a clear decrease of the hormone-producing activity in the ovaries of fetuses from mothers with HILGT comparing to such indexes in fetal organs of fetuses from mothers with physiological pregnancy. The complex of changes in the ovaries of fetuses with a 21-28 weeks of gestational term, which was described, was formed under the influence of the chronic hypoxia, prolonged antigenic stimulation as well as hormonal violations in the mother-placenta-fetus system, which is a leading link of the infectious pathology’ pathogenesis. The aforementioned complex of the structural and functional changes in the ovaries of fetuses from mothers with HILGT in a gestational term of 21-28 weeks indicates violations in implementation and formation of the fetal gonads, as well as it could further lead to disorders of the germinal function in the female organism.

scholarly journals Immunohistochemical features of the fallopian tubes’ structure in case of fetuses with a gestational term of 21-28 weeks from mothers with a chronic infection of the lower genital tracts

2021 ◽  
Vol 11 (8) ◽  
pp. 445-456
Author(s):  
V. Poliasnyi ◽  
L. Kupriianova
Keyword(s):  
Mucous Membrane ◽  
Chronic Infection ◽  
Main Group ◽  
Immunohistochemical Method ◽  
Fallopian Tubes ◽  
Tubal Infertility ◽  
Inflammatory Infiltration ◽  
Endocrine Activity ◽  
Endocrine Insufficiency ◽  
Complicated Pregnancy

In the following article we are disclosing immunohistochemical features of the fallopian tubes’ structure in case of fetuses with a gestational term of 21-28 weeks. We have studied the structure of organs of 15 fetuses from mothers with a chronic infection of the lower genital tracts (HILGT) (the group of comparison) relatively to one in case of 15 fetuses from mothers with a physiological pregnancy (the main group). All fetuses had a gestational term of 21-28 weeks and had died intranatally as a result of an acute disorder of uterine-placental circulation. Methods of reearch: organometric, histological, immunohistochemical and statistical. The complex research allowed to reveal a probable decrease of the organometric data as well as indicators of thickness of the main structural components of the organs’ wall in case of fetuses from mothers of the group of comparison relatively to ones in case of fetuses from the main group. By applying histological method we had reveled a disorder in the strucuture of mucous and muscular membranes in the structure of organs of fetuses from mothers with a pathology of pregnancy, which is manifested by decreasing of number of folds, uneven thickness of the mucous membrane as well as disorder in formation of the muscular membrane’ layers. Immunohistochemical method has revealed an increase of apoptotically changed mucosal cells in the fallopian tubes of fetuses from mothers with HILGT. By applying MCAT to endotheline-1 we have revealed an increased glow of endithelial cells in vessels both of arterial and venous types in the strucuture of the organs’ wall in case of fetuses from the group of comparison. During the study on the endocrine activity of organs of fetuses from mothers with complicated pregnancy we have postulated a probable decrease of the cells’ glow’ intensity towards MCAT of progesterone. At the same time, there is also an inflammatory infiltration in the mucous membrane of the fallopian tubes’ wall. The aforementioned changes in the strucuture as well as in the functional activity of fetuses’ organs from mothers’ with HILGT are formed under the influence of chronic hypoxia and endocrine insufficiency of the feto-placental complex, which is taking place in case of this pathology. Histological and immunohistochemical features of the fallopian tubes’ structure in case of fetuses from mothers with complicated pregnancy are indicating a disorder in formation and maturing of the organ as well as they could lead to development of ectopic pregnancy and tubal infertility in the subsequent ontogenesis.

RESULTS OF LOWER GENITAL TRACT INFECTION INTERVENTION AMONG KHMER WOMEN OF CHILDBEARING AGE IN CAN THO IN 2016

2018 ◽  
pp. 83-89
Author(s):  
Trung Thuan Phan ◽  
Dinh Binh Tran ◽  
Thanh Hue Dinh ◽  
Phong Son Dinh ◽  
Kieu Oanh Truong ◽  
...  
Keyword(s):  
Genital Tract ◽  
Common Knowledge ◽  
Intervention Group ◽  
Effective Intervention ◽  
Control Group ◽  
Childbearing Age ◽  
Lower Genital Tract ◽  
Genital Tract Infections ◽  
Tract Infections ◽  
Can Tho City

Objectives: To find a model that is effective intervention to reduce the incidence of lower genital tract infections in Khmer women. Subjects and Methods: The intervention study is carried which compared the test group with the control group of 400 Khmer women, aged 15 to 49 in Can Tho city. Results: The common knowledge of the prevention of lower genital tract infections in the intervention group increased from 30.9% in the control group to 54.5% in the intervention group. The general attitude toward the use of lower genital tract infections increased from 22.3% in the control group to 78.0% in the intervention group. This is a very significant improvement. The prevalence of subclinical infection of the intervention group (26.0%) was significantly lower than that of the control group (39.3%), the difference was statistically significant. Conclusion: Intervention effectiveness is good in both knowledge, attitudes and practices, especially the effectiveness of interventions in practice in Khmer women. Key words: Effective intervention, lower genital tract infections, Khmer women, Can Tho city

scholarly journals Association of Lower Genital Tract Inflammation With Objective Evidence of Endometritis

2000 ◽  
Vol 8 (2) ◽  
pp. 83-87 ◽  
Author(s):  
Jeffrey F. Peipert ◽  
Roberta B. Ness ◽  
David E. Soper ◽  
Debra Bass
Keyword(s):  
Tract Infection ◽  
Blood Cells ◽  
Genital Tract ◽  
White Blood Cells ◽  
Genital Tract Infection ◽  
Lower Genital Tract ◽  
Objective Evidence ◽  
Group 2 ◽  
Upper Genital Tract Infection ◽  
Group 1

The purpose of this report is to evaluate the association between lower genital tract inflammation and objectively diagnosed endometritis. We analyzed the first 157 patients enrolled in the PEACH study, a multicenter randomized clinical trial designed to compare the effectiveness of outpatient and inpatient therapy for PID. Women less than 38 years of age, who presented with a history of pelvic discomfort for 30 days or less and who were found to have pelvic organ tenderness (uterine or adnexal tenderness) on bimanual examination, were initially invited to participate. After recruitment of the first 58 patients (group 1) we added the presence of leukorrhea, mucopurulent cervicitis, or untreated positive test forN. gonorrhoeaeorC. trachomatisto the inclusion criteria (group 2, N = 99). We compared rates of endometritis in the two groups and calculated the sensitivity, specificity, and predicted values of the presence of white blood cells in the vaginal wet preparation. The rate of upper genital tract infection in group 1 was 46.5% (27/58) compared to 49.5% (49/99) in group 2. Microbiologic evidence of eitherN. gonorrhoeaeorC. trachomatisincreased from 22.4% in group 1 to 38.3% in group 2. The presence of Vaginal white blood cells or mueopus has a high sensitivity (88.9%), but a low specificity (19.4%) for the diagnosis of upper genital-tract infection. Assessment of the lower genital tract for evidence of infection or inflammation is a valuable component of the diagnostic evaluation of pelvic inflammatory disease. The presence of either mucopus or vaginal white blood cells is a highly sensitive test for endometritis in patients with pelvic pain and tenderness. Infect. Dis. Obstet. Gynecol. 8:83–87, 2000.

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