scholarly journals Immunohistochemical features of the fallopian tubes’ structure in case of fetuses with a gestational term of 21-28 weeks from mothers with a chronic infection of the lower genital tracts

2021 ◽  
Vol 11 (8) ◽  
pp. 445-456
Author(s):  
V. Poliasnyi ◽  
L. Kupriianova
Keyword(s):  
Mucous Membrane ◽  
Chronic Infection ◽  
Main Group ◽  
Immunohistochemical Method ◽  
Fallopian Tubes ◽  
Tubal Infertility ◽  
Inflammatory Infiltration ◽  
Endocrine Activity ◽  
Endocrine Insufficiency ◽  
Complicated Pregnancy

In the following article we are disclosing immunohistochemical features of the fallopian tubes’ structure in case of fetuses with a gestational term of 21-28 weeks. We have studied the structure of organs of 15 fetuses from mothers with a chronic infection of the lower genital tracts (HILGT) (the group of comparison) relatively to one in case of 15 fetuses from mothers with a physiological pregnancy (the main group). All fetuses had a gestational term of 21-28 weeks and had died intranatally as a result of an acute disorder of uterine-placental circulation. Methods of reearch: organometric, histological, immunohistochemical and statistical. The complex research allowed to reveal a probable decrease of the organometric data as well as indicators of thickness of the main structural components of the organs’ wall in case of fetuses from mothers of the group of comparison relatively to ones in case of fetuses from the main group. By applying histological method we had reveled a disorder in the strucuture of mucous and muscular membranes in the structure of organs of fetuses from mothers with a pathology of pregnancy, which is manifested by decreasing of number of folds, uneven thickness of the mucous membrane as well as disorder in formation of the muscular membrane’ layers. Immunohistochemical method has revealed an increase of apoptotically changed mucosal cells in the fallopian tubes of fetuses from mothers with HILGT. By applying MCAT to endotheline-1 we have revealed an increased glow of endithelial cells in vessels both of arterial and venous types in the strucuture of the organs’ wall in case of fetuses from the group of comparison. During the study on the endocrine activity of organs of fetuses from mothers with complicated pregnancy we have postulated a probable decrease of the cells’ glow’ intensity towards MCAT of progesterone. At the same time, there is also an inflammatory infiltration in the mucous membrane of the fallopian tubes’ wall. The aforementioned changes in the strucuture as well as in the functional activity of fetuses’ organs from mothers’ with HILGT are formed under the influence of chronic hypoxia and endocrine insufficiency of the feto-placental complex, which is taking place in case of this pathology. Histological and immunohistochemical features of the fallopian tubes’ structure in case of fetuses from mothers with complicated pregnancy are indicating a disorder in formation and maturing of the organ as well as they could lead to development of ectopic pregnancy and tubal infertility in the subsequent ontogenesis.

scholarly journals Immunohistochemical features of the structure of fetuses’ uterus with a gestational term of 21-28 weeks from mothers, whose pregnancy is complicated by chronic infection of the lower genital tract

2021 ◽  
Vol 11 (2) ◽  
pp. 230-245
Author(s):  
V. O. Polyasnyi ◽  
L. S. Kupriianova
Keyword(s):  
Connective Tissue ◽  
Genital Tract ◽  
Chronic Infection ◽  
Medical Science ◽  
Uterine Wall ◽  
Main Group ◽  
Immunohistochemical Method ◽  
Endocrine Disorders ◽  
Lower Genital Tract ◽  
Complicated Pregnancy

The relevance of the following article is prescribed by the growing number of perinatal mortality and morbidity among newborns due to the mother’s chronic infection of the lower genital tract (HILGT). It is commonly known, that HILGT contributes to the delay of the fetal development likewise is often manifested by the violation of internal organs and systems implementation. One of the most acute topic is the the fetus’ reproductive system in ther early gestation terms. However, the immunohistochemical features of the fetuses’ uterus structure with the gestational term of 21-28 weeks from mothers with HILGT on the current stage of the medical science develpment have not been described yet. The aim of the current research is to identify immunohistochemical features of the fetuses’ uterus structure with the term of gestation of 21-28 weeks from mothers, whose pregnancy is complicated by HILGT. Material of the research: 25 fetuses from mothers, whose course of pregnancy was physiological (group of comparison) as well as 25 fetuses from mothers, whose pregnancy was complicated by HILGT (main group). All fetuses had died intranatally, as a result of acute uterine placental and placental-fetal circulation. Research methods: organometric, histological, immunohistochemical, morphometric, statistical. The research results and their discussion: the organometric parameters of the fetuses’ uterus from the main group were clearily decreased in comparison to such parameters in case of the group of comparison. The decrease of the parameter of the uterine wall thickness of fetuses from mothers with the complicated pregnancy prescribes thinning of the main structural components. Namely: endometrium, myometrium and perimetrium. In case of endometrium of fetuses’ uterus a decrease of number of glands and the lack of their functional activity was noticed. It characterizes this layer as structurally immature.At the same time, in case of fetal organs of fetuses from mothers with complicated pregnancies, we could notice an increase in the number of apoptotically altered cells. Comparing to the structure of layers of uterine walls in case of fetuses from mothers with physiological pregnancy, in the uterus of fetuses from mothers with complicated pregnancy our attention is attracted by a massive growth of the connective tissue. In the myometrium it contributes to the disruption of the muscle fibers strusture likewise to its absence in some areas. By the immunohistochemical method it was possible to reveal a prevalence of the collagen of the III type in the structure of connective tissue likewise in the walls of blood vessels. It is connected primarily with disruption of the collagen’ maturation. The vascular component of the uterine wall in case of fetuses from mothers with HILGT differs from ones in case of fetuses from mothers with physiological pregnancy. This difference is grounded on the increase of endothelin-producing activity by vessels of both arterial and venous types. By processing preparations by monoclonal antibodies to estrogen, it has been postulated, that the intensity of the reaction as well as number of cells in the fetuses’ organs of the main group are clearily decreased comparing to ones in cases of fetuses from the group of comparison. The disorders of the fetuses’ uterus in case of fetuses from mothers with HILGT, that were provided in the current article, are prescribed by chronic hypoxia, endocrine disorders as well as influences of the infectious agents, that are taking place in case of such pathology. The characteristic features of the fetuses’ uterus structure in case of fetuses from mothers, whose pregnancy is complicated by HILGT, indicate gross violation in the organ’s implementation on the early stages of the fetal organogenesis, likewise they could be a leading link of pathogenesis of primary infertility’ development in the future ontogenesis.

scholarly journals Immunuhistochemical features of the ovaries’ structure in case of fetuses with a gestational term of 21-28 weeks from mother, whose pregnancy was complicated by chronic infections of lower genital tracts

2021 ◽  
Vol 11 (5) ◽  
pp. 246-261
Author(s):  
V. O. Poliyasnyi ◽  
L. S. Kupriianova
Keyword(s):  
Connective Tissue ◽  
Main Group ◽  
Relative Volume ◽  
Immunohistochemical Method ◽  
Chronic Infections ◽  
Functional Changes ◽  
Massive Growth ◽  
Cortical Substance ◽  
Complicated Pregnancy ◽  
Clear Increase

The following article presents results of the complex research on postulation of immunohistochemical features of the ovaries’ structure in case of fetuses with a gestational term of 21-28 weeks from mothers with HILGT comparing to ones in case of fetuses from mothers with a physiological pregnancy. The materials of the research are: 25 fetuses from mothers with HILGT as well as 25 fetuses from mothers with physiological pregnancy. All fetuses had died antenatally and inranatally, as a result of violation of uteroplacental and umbilical-uterine circulation. Research methods: macroscopic, organometric, histological, immunohistochemical, morphometric, statistical. The organometric method revealed a clear decrease of main indexes of the fetuses’ ovaries from mothers with a complicated pregnancy comparing to ones in case of organs of fetuses from healthy mothers. Morphometric method revealed a clear decrease of a relative volume of cortical substance as well as a clear increase of a relative volume of brain substance in the gonads of fetuses from the main group; as well as it showed a clear decrease of an index of follicular tissue’ relative volume, likewise a clear increase of an index of interstitial component relative volume.The staining by picrofuxin by van Gieson method revealed a massive growth of the connective tissue in all strucutral components of organs of fetuses from mothers with HILGT relatively to gonads of fetuses from healthy mothers. Using of MCAT to main types of collagens allowed to reveal a violation of maturation of main types of collagens in the structure of connective tissue of fetal gonads in the main group. In the follicular component of the fetuses’ ovaries in the main group are appearing mature, cystic-artretic and growing forms of follicles. Immunohistochemical method revealed an increased number of an apoptotically altered eggs in the ovaries of fetuses from mothers with HILGT comparing to one in organs of fetuses from mothers with physiological pregnancy.  The peroxidase method revealed an increased endotheline-producing activity of the vascular component as arterial, like a venous type; likewise a clear decrease of the hormone-producing activity in the ovaries of fetuses from mothers with HILGT comparing to such indexes in fetal organs of fetuses from mothers with physiological pregnancy. The complex of changes in the ovaries of fetuses with a 21-28 weeks of gestational term, which was described, was formed under the influence of the chronic hypoxia, prolonged antigenic stimulation as well as hormonal violations in the mother-placenta-fetus system, which is a leading link of the infectious pathology’ pathogenesis. The aforementioned complex of the structural and functional changes in the ovaries of fetuses from mothers with HILGT in a gestational term of 21-28 weeks indicates violations in implementation and formation of the fetal gonads, as well as it could further lead to disorders of the germinal function in the female organism.

In vitro fertilization outcome in women with tubal factor of infertility and previous chlamydial infection

2014 ◽  
Vol 63 (4) ◽  
pp. 54-59
Author(s):  
Valeriya Sergeyevna Myuller ◽  
Igor Yuryevich Kogan ◽  
Alevtina Mikhaylovna Savicheva
Keyword(s):  
Live Birth ◽  
Follicular Fluid ◽  
Chlamydial Infection ◽  
Comparison Group ◽  
Main Group ◽  
Previous Exposure ◽  
Miscarriage Rate ◽  
Tubal Infertility ◽  
Tubal Factor

In order to examine the results of IVF and IVF/ICSI programs in women with tubal infertility (TFI) and previous exposure to chlamydial infection a total of 242 women were enrolled in the study. Materials and methods. Sera and follicular fluid samples were tested using EIA for antibodies to chlamydial antigens: the major outer membrane protein (MOMP - IgG, IgA) and heat shock protein chsp60 (IgG). Prevalence of antichlamydial antibodies in sera was 50,4 % (122/242), 10,7 % (26/242) and 5,8 % (14/242); in follicular fluid - 30,2 % (73/197), 3,7 % (9/197) and 4,5% (11/197) coordinaly. Patients were divided to two groups: main group (with IgG to C. trachomatis MOMP in sera, N = 122) and comparison group (absence of antibodies, N = 120). Results. The miscarriage rate after conventional IVF or IVF/ICSI cycle in main group patients was 28 % (7/30), compared to 3,3 % (1/30) in comparison group, р = 0,018. Pregnancy and live birth rate in women from main group was 40 % (22/55) and 30,9 % (17/55); in patients of comparison group - 22,2 % (16/72) and 15,3 % (11/72); р = 0,03 and р = 0,035, coordinaly. Results showed no difference in efficiency of IVF/ICSI programs for the examined groups of patients. Conclusion. Antibodies to C. trachomatis antigens are detected in blood serum of half, in follicular fluid - of every third patient with tubal factor of infertility. Serological evidence of previous exposure to chlamydial infection in these women is associated with decreased IVF success - lower pregnancy and live birth rates and higher risk of miscarriage.

Surgical Treatment of the Tubal Infertility: Issues and Options

10.12737/3303 ◽  
2014 ◽  
Vol 21 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Яковлева ◽  
Natalya Yakovleva
Keyword(s):  
Surgical Treatment ◽  
Surgical Management ◽  
High Efficiency ◽  
Assisted Reproductive Technologies ◽  
Adhesion Formation ◽  
Reproductive Technologies ◽  
Postoperative Adhesion ◽  
Fallopian Tubes ◽  
Tubal Infertility ◽  
Surgical Methods

There are two basic principles in the treatment of the tubal infertility: surgery and assisted reproductive technologies. The high efficiency of the surgical methods of the female reproductive functions recovery in comparison with in vitro fertilization is demonstrated in this paper. According to the literature review there are two effective basic surgical methods: microsurgery and endo-video-surgery in the treatment of the patients with tubal infertility. It was demonstrated that the unsuccessful results of the surgical treatment of the patients with tubal infertility are essentially connected to the fallopian tubes reocclusion and adhesive process recurrence in the small pelvis cavity. According to the new literature data, the different intra-operative and postoperative methods of the improvement of the effectiveness of the surgical management of this patient population were analyzed. However the further examinations are necessary, they will determine strict criteria for the treatment method choice of the patients with tubal infertility. The possible ways to increase the surgical management efficiency of the tubal infertility will be the surgical technique development and working out of the new methods of the rehabilitation therapy oriented to the prophylaxis of the fallopian tubes reocclusion, restoration of their functional activity, prophylaxis of the postoperative adhesion formation. The literature data review showed that surgical treatment and assisted reproductive technology must be considered as complement each other methods that may be used particularly and in combination.

scholarly journals MORPHOLOGICAL CHANGES IN THE SMALL INTESTINE OF RATS WITH ACUTE GENERALIZED PERITONITIS

2019 ◽  
pp. 110-113
Author(s):  
I. Ya. Dzyubanovsky ◽  
B. M. Vervega ◽  
S. R. Pіdruchna ◽  
N. A. Melnyk
Keyword(s):  
Endothelial Cells ◽  
Small Intestine ◽  
Mucous Membrane ◽  
Morphological Changes ◽  
Histological Study ◽  
Abdominal Cavity ◽  
Inflammatory Infiltration ◽  
Generalized Peritonitis ◽  
White Rats ◽  
Fecal Suspension

The main cause of mortality in acute generalized peritonitis (AGP) is the development of multiple organ insufficiency. The intestine is the organ where the first changes develop in this pathology. The aim of the study – to research and evaluate the morphological changes in the small intestine wall of animals with experimental AGP. Materials and Methods. 32 white rats were used in this study. Acute peritonitis was modeled by introduction of 10 % fecal suspension in the dosage of 0.5 ml per 100 g of the animal's weight into the abdominal cavity of rats by puncture. The terms of observation: the 1st, 3rd and the 7th days from the beginning of the peritonitis modeling. For histological study the intestinal tissue was taken. The resulting pieces of the organ were fixed in a 10 % neutral formalin solution, which were then stained with hematoxylin and eosin. Results and Discussion. On the 3rd day of the experiment in animals with a modeled AGP, vascular changes were manifested first of all by the rounding (retraction) of endothelial cells or their desquamation and the appearance of defects, that allow plasma proteins and the formed elements of blood to leave circulation boundaries of the vascular bed. On the 7 th day in animals with a modeled AGP increased vascular permeability of the mucous membrane of the small intestine was accompanied by a significant edema of the stroma of the villi and by focal hemorrhages. Conclusion. Consequently, the distinct inflammatory changes in all terms of the injury were seen in the wall of the small intestine at the simulated AGP. The significant expansion of the capillaries and venules against the background of inflammatory infiltration in the stroma of the glandular component of the small intestine was noted on the 1st day from the beginning of the experiment. An increase in the height of intestinal villus and a crypt with retraction and desquamation of endothelial cells in the wall of vessels, which caused platelet adhesion in the areas of destruction was observed on the 3rd day. The areas of focal necrosis of the superficial epithelium, which were accompanied by multiple hemorrhages per diapedesis in the perivascular space of the mucous membrane, were seen in the wall of the small intestine of animals with a simulated AGP on the 7th day.

Surrogate human Fallopian tubes for overcoming tubal infertility

1989 ◽  
Vol 4 (4) ◽  
pp. 416-417 ◽  
Author(s):  
L. Formigli ◽  
M. Pagano ◽  
C. Roccio ◽  
A. Stangalini ◽  
G. Belotti ◽  
...  
Keyword(s):  
Fallopian Tubes ◽  
Tubal Infertility

scholarly journals African experience of hysterosalpingography abnormalities tubes management by laparoscopy in infertile women

2019 ◽  
Vol 8 (11) ◽  
pp. 4271
Author(s):  
Mohamed Fanny ◽  
Edele Aka ◽  
Perel Konan ◽  
Luc Olou ◽  
Abdoul K. Koffi ◽  
...  
Keyword(s):  
Pelvic Surgery ◽  
Fallopian Tubes ◽  
Tubal Infertility ◽  
Tubal Patency ◽  
Infertile Women ◽  
African Experience ◽  
Gynecology And Obstetrics ◽  
Therapeutic Procedures ◽  
History Of ◽  
Sub Saharan

Background: Infertility affects about 80 million people worldwide and one in ten couples. The objective of this study was to report our experience of the contribution of laparoscopy in the diagnostic and prognostic approach of fallopian tubes pathology in infertile women in sub-Saharan Africa.Methods: We conducted a retrospective study in the Gynecology and Obstetrics unit of Yopougon Teaching Hospital over a 2-year period (January 1, 2017 to December 31, 2018) which included 49 cases of tubo-peritoneal infertility diagnosed by hysterosalpingography (HSG) then laparoscopy procedure.Results: The average age was 33 years old.  30.6 % were single. 75% had secondary infertility. 59.2% had a medical history of abortion. Pelvic Inflammatory Disease and pelvic surgery accounted 84.2% and 49 respectively. Laparoscopy showed a predominance of distal tubal damage (66.6%) whose 47% hydrosalpinx. Bilateral tubal patency was demonstrated in 77.5% of our patients during laparoscopy procedure. We observed a sensitivity, a specificity, and a concordance laparoscopy / HSG of 63.6%, 80% 63.1% respectively. Laparoscopy also allowed therapeutic procedures such as adhesiolysis or tubal plasty in 22 patients (44.89% of our cases). At the end of laparoscopy procedure, 35 patients (71.42%) were turned towards IVF.Conclusions: Laparoscopy allows an assessment of tubal abnormalities revealed by hysterosalpingography and the fertility prognostic as well as better therapeutic approach in management of tubal infertility.

Our Experience in the Treatment of Bacterial Keratitis

2021 ◽  
pp. 27-33
Author(s):  
V. M. Sakovych ◽  
O. V. Aleksieieva
Keyword(s):  
Hyperbaric Oxygenation ◽  
Corneal Edema ◽  
Main Group ◽  
Control Group ◽  
Comprehensive Treatment ◽  
Clinical Parameters ◽  
Bacterial Keratitis ◽  
Bacterial Strains ◽  
Inflammatory Infiltration ◽  
And Control

Introduction. In recent years, the emergence of bacterial strains resistant to antibiotics used in medical practice significantly reduced the effectiveness of chemoprophylaxis and therapy. Following data indicates the need to search for new antibacterial drugs and ways of treating patients with bacterial keratitis. The aim of research is improvement of clinical parameters in patients with bacterial keratitis using hyperbaric oxygenation. Materials and Methods. We observed 68 patients (68 eyes) with bacterial keratitis aged 18 to 75 years. There were 36 men and 32 women. The patients were divided into 2 groups: main group (38 patients) and control group (30 patients). All the patients received antibiotic therapy, mydriatics, tissue-based products, vitamins. In addition, patients of the main group underwent hyperbaric oxygenation. The severity of the inflammatory response was evaluated using objective indicators: the presence and type of discharge into the conjunctival cavity, the severity of the mixed injection, corneal edema, the degree of inflammatory infiltration of the surface layers of the cornea. Results. The changes of clinical parameters in patients of the main and control groups were evaluated and scored (conjunctival discharge in the cavity, the severity of the mixed injection, corneal edema and inflammatory infiltration). The therapy contributed to faster disappearance of discharge into the conjunctival cavity and mixed injection, regression of edema, resorption of infiltrates (p <0.001). Conclusions. Hyperbaric oxygenation in comprehensive treatment of patients with bacterial keratitis is highly efficient, provides stable clinical and functional results in the main group compared with the control group. Keywords: bacterial keratitis, hyperbaric oxygenation, comprehensive treatment, clinical parameters in points.

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