scholarly journals Efficiency of ademethionine in oxidative stress in tissues of irradiated rats

2021 ◽  
Vol 11 (6) ◽  
pp. 192-198
Author(s):  
G. F. Stepanov ◽  
L. O. Tereshchenko ◽  
E. V. Oleinik ◽  
G. S. Maryniuk ◽  
O. I. Budalenko ◽  
...  
Keyword(s):  
Total Dose ◽  
Control Level ◽  
Experimental Studies ◽  
Living Organism ◽  
Reduction Factor ◽  
Low Doses ◽  
Pyridine Nucleotides ◽  
Γ Irradiation ◽  
Low Intensity ◽  
Reduced Forms

Introduction. Ionizing radiation in low doses of low intensity causes prolonged activation of lipid per oxidation and depletion of the antioxidant system in a living organism. Moreover, Ademethionine is currently being considered as a promising antioxidant.Method. Experimental studies were carried out on 60 sexually mature male Wistar rats. The animals were irradiated in a total dose of 1Gy on a γ-therapeutic device AGAT-R No. 83 (isotope 60Co). At the end of the total dose, the rats were injected intraperitoneally with Heptral (ademethionine) after 15 minutes, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, 144, 156 hours after radiation exposure at the rate of 10 mg / kg mass. After the introduction of Heptral, the animals were taken into the experiment after 24 hours, 3, 7, 15 days. In homogenates of the spleen and thymus of animals, the amount of oxidized and reduced forms of pyridine nucleotides was determined.Results. Chronic γ-irradiation in a total dose of 1Gy leads to a significant decrease in the content of reduced forms of pyridine nucleotides in the spleen and thymus of rats. Administration of Heptral to irradiated animals normalized oxidative homeostasis. So, on the 7th day of the experiment, the amount of oxidized forms of pyridine nucleotides in the spleen was 47.3% lower, and reduced - 36.3% higher than in animals that did not receive treatment. At the end of the observation period, the reduction coefficient of pyridine nucleotides in the spleen slightly differed from the control level. In comparison with irradiated animals, which were not injected with Heptral, the NADP content was lower by 70.3%, and NADPH2 - higher by 48.8%.Conclusion. The course administration of Heptral to irradiated animals leads to the normalization of the reduction factor of pyridine nucleotides. According to its mechanism of action, Heptral can be used in the complex treatment of low- intensity radiation injuries in low doses.

MORPHOFUNCTIONAL PARAMETERS OF CATTLE ERYTHROCYTES UNDER STRESS AND ITS CORRECTION BY LOW-INTENSITY LASER RADIATION

1970 ◽  
pp. 67-71
Author(s):  
М. N. Ivashchenko ◽  
А. V. Deryugina ◽  
P. S. Ignatiev ◽  
V. B. Metelin ◽  
М. N. Talamanova ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Erythrocyte Membrane ◽  
Control Level ◽  
Experimental Studies ◽  
Erythrocyte Membranes ◽  
Physical Factors ◽  
Low Intensity ◽  
Intensity Laser ◽  
Technological Stress

The study investigated the content of malondialdehyde (MDA), the electrophoretic mobility of erythrocytes (EPME), the spectrum of erythrocyte membrane proteins and the morphology of cattle erythrocytes in in vitro experiments under stress and exposure to low-intensity laser radiation (LLLT). Clinical and experimental studies carried out in the last decade indicate the possibility of modulating the organism adaptive reactions when exposed to such physical factors as low-intensity laser radiation. The work showed that the effect of LLLT on the blood of stressed animals caused the restoration of the studied parameters to the level of physiological norms, while in animals that underwent technological stress, EPME was reduced by 31%, MDA concentration was increased by 65%. The effect of LLLT on the blood of unstressed animals did not lead to a change in EPME and MDA concentration. The study of the protein spectrum of erythrocyte membrane of animals subjected to technological stress revealed that the content of spectrin decreased by 16%, glycophorin C increased by 35%, the morphology of erythrocytes after stress was characterized by an increase in the number of echinocytes, stomatocytes and degeneratively altered erythrocytes. Under the LLLT action on the cow erythrocytes after stress there was a restoration of the morphology of cells and the content of proteins of erythrocyte membranes to the control level.

Calculation-and-experimental estimation of the role of the frequency ratio in changing the endurance at two-frequency deformation modes

2019 ◽  
Vol 85 (1(I)) ◽  
pp. 64-71 ◽  
Author(s):  
M. M. Gadenin
Keyword(s):  
High Frequency ◽  
Experimental Studies ◽  
Low Frequency ◽  
Reduction Factor ◽  
Single Frequency ◽  
Cyclic Loads ◽  
Small Ratio ◽  
Harmonic Components ◽  
Deformation Modes

The cycle configuration at two-frequency loading regimes depends on the number of parameters including the absolute values of the frequencies and amplitudes of the low-frequency and high-frequency loads added during this mode, the ratio of their frequencies and amplitudes, as well as the phase shift between these harmonic components, the latter having a significant effect only with a small ratio of frequencies. Presence of such two-frequency regimes or service loading conditions for parts of machines and structures schematized by them can significantly reduce their endurance. Using the results of experimental studies of changes in the endurance of a two-frequency loading of specimens of cyclically stable, cyclically softened and cyclically hardened steels under rigid conditions we have shown that decrease in the endurance under the aforementioned conditions depends on the ratio of frequencies and amplitudes of operation low-frequency low-cycle and high-frequency vibration stresses, and, moreover, the higher the level of the ratios of amplitudes and frequencies of those stacked harmonic processes of loading the greater the effect. It is shown that estimation of such a decrease in the endurance compared to a single frequency loading equal in the total stress (strains) amplitudes can be carried out using an exponential expression coupling those endurances through a parameter (reduction factor) containing the ratio of frequencies and amplitudes of operation cyclic loads and characteristic of the material. The reduction is illustrated by a set of calculation-experimental curves on the corresponding diagrams for each of the considered types of materials and compared with the experimental data.

scholarly journals Prospects of using extremely low doses of physical factors impact in food biotechnology

2018 ◽  
Vol 6 (2) ◽  
pp. 305-313 ◽  
Author(s):  
Tatiana Danilchuk ◽  
Tatiana Danilchuk ◽  
Vera Ganina ◽  
Vera Ganina
Keyword(s):  
Hydrolytic Enzymes ◽  
Physical Factor ◽  
Maximum Activity ◽  
Physical Factors ◽  
Experimental Sample ◽  
Low Doses ◽  
Resource Saving ◽  
Food Biotechnology ◽  
Low Intensity ◽  
Biotechnological Processes

The paper deals with urgent issues of development of resource-saving methods of biotechnological processes intensification. The main purpose of the work is to show the efficiency of using extremely low doses of physical factors impact in food biotechnology. Acoustic and electric treatment was in various modes. Impact capacity did not exceed 10-4 W/kg. The duration of exposure ranged from 5 to 15 min. Barley grains enzymes and lactic acid microorganisms were subjected to treatment. Impact of vibration as a physical factor, its frequency is considered as a priority in controlling growth and biochemical processes in biological objects. Impact frequency in the range of 50–10000 Hz influences the activity of hydrolytic enzymes in bimodal way. It is presented in the article. The coincidence of the frequency ranges of the maximum activity of enzymes in the model reactions with the ones in the grain of barley under the treatment of alternating current and sound is noted. Improvement of all indexes of germinating barley and improvement of the quality of the finished malt were observed in these ranges. Low-intensity acoustic treatment at a frequency of 2000 Hz contributed to an increase in β-galactosidase activity of the CT-95 Str. thermophilus strain. Selected ST-95 Str. thermophilus strain was used in the starter composition for the experimental sample production of fermented melted milk. The fermentation process intensification was observed. The lactose content of this product is 30% less in comparison with the feedstock. The use of traditional starter without a selected strain can reduce the lactose content by an average of 7.5%. The results of biotechnological processes modification by means of the treatment of enzymes and extremely low doses of physical factors impact used in meat technologies are observed. Thus, the prospects of using low-intensity physical effects of vibration in the development of innovative food technologies are substantiated. It is also promising to use these technologies in making absolutely new food products with different qualities.

scholarly journals Experimental Studies and the Search for New Innovative Approaches to Treat Dry Eye Disease

2021 ◽  
Vol 11 (5) ◽  
pp. 294-302
Author(s):  
Anastasiia Liemienieva ◽  
Iryna Shargorodska ◽  
Sergiy Rykov ◽  
Mykola Petrovsky
Keyword(s):  
Dry Eye ◽  
Red Light ◽  
Control Level ◽  
Experimental Studies ◽  
Light Therapy ◽  
Dry Eye Disease ◽  
Eye Disease ◽  
Anterior Surface ◽  
Tear Production ◽  
Serious Disease

Introduction and purpose. The main manifestation of eyelids, tear ducts, and orbits disorders is a dry eye disease considered as a serious disease that affects the quality of life. Nowadays, one in ten people worldwide faces this disease. Increased workload, development of multimedia technologies, hypodynamia, and environmental degradation are the main modern causes of the disease. Material and methods. In this work, we used an experimental model of dry eye in rats with the development of inflammation of the anterior surface of the eye. Alkali burns were used to develop inflammation. A fluorescein test performed immediately after alkaline erosion and on days 1, 5, and 10 after burns revealed a gradual increase in corneal epithelialization and vascularization. Results. One day after induction of inflammation, there was a dramatic and statistically significant increase in tear production in experimental rats. By the seventh day, tear production declined to the control level with a subsequent tendency to decrease tear production. Conclusions. In the group of rats with inflammation of the anterior surface of the eye that received polarized red-light therapy, there was no significant decrease in tear production on day 7, which may be indicative of a possible anti-inflammatory effect of light therapy under these conditions.

Effects of the total dose and duration of γ-irradiation on the growth responses and induced SNPs of a Cymbidium hybrid

2020 ◽  
Vol 96 (4) ◽  
pp. 545-551 ◽  
Author(s):  
Sang Hoon Kim ◽  
Yeong Deuk Jo ◽  
Jaihyunk Ryu ◽  
Min Jeong Hong ◽  
Byoung-Cheorl Kang ◽  
...  
Keyword(s):  
Total Dose ◽  
Growth Responses ◽  
Γ Irradiation

Experimental studies on the metabolism of glucose and pyridine nucleotides in liver injuries

1967 ◽  
Vol 2 (2) ◽  
pp. 136-137
Author(s):  
K. Kuzuya ◽  
K. Mori ◽  
S. Tabuchi ◽  
K. Hamana ◽  
H. Inoue ◽  
...  
Keyword(s):  
Experimental Studies ◽  
Pyridine Nucleotides ◽  
Liver Injuries

Preservation of Cheese and Plain Yogurt by Low-dose Irradiation

1980 ◽  
Vol 43 (2) ◽  
pp. 114-118 ◽  
Author(s):  
S. YÜCEER ◽  
G. GÜNDÜZ
Keyword(s):  
Side Effects ◽  
Shelf Life ◽  
Total Dose ◽  
Room Temperature ◽  
Low Dose ◽  
Sorbic Acid ◽  
Low Doses ◽  
Dose Irradiation ◽  
Refrigerator Temperature ◽  
Number Of Bacteria

Irradiation preservation of Turkish kashar cheese and plain yogurt was studied using very low doses of Co-60 radiation. No side effects were observed below 0.15 Mrad. The number of bacteria killed was directly related to total dose up to 0.02 Mrad, then the effectiveness of the dose decreased. Coating cheese samples with a sorbic acid solution helped in reducing the number of bacteria by about 10–12% in irradiated samples. The shelf-life of irradiated samples stored at refrigerator temperature was almost the same as that of ones coated with sorbic acid but stored at room temperature. Mold formation in irradiated samples took three to four times longer than in nonirradiated ones. With plain yogurt the effect of total dose seemed to be the same as in cheese. Irradiation increased the shelf-life of yogurt three- to four-fold. Preservation by irradiation combined with refrigeration increased the shelf-life about five-fold.

Experimental studies of direct-drive, low-intensity, low-adiabat spherical implosions on OMEGA

2007 ◽  
Vol 14 (2) ◽  
pp. 022702 ◽  
Author(s):  
V. A. Smalyuk ◽  
R. Betti ◽  
J. A. Delettrez ◽  
V. Yu. Glebov ◽  
V. N. Goncharov ◽  
...  
Keyword(s):  
Experimental Studies ◽  
Direct Drive ◽  
Low Intensity

scholarly journals Dynamic Penumbra Demonstrated by Sequential Multitracer PET after Middle Cerebral Artery Occlusion in Cats

1994 ◽  
Vol 14 (6) ◽  
pp. 892-902 ◽  
Author(s):  
W.-D. Heiss ◽  
R. Graf ◽  
K. Wienhard ◽  
J. Löttgen ◽  
R. Saito ◽  
...  
Keyword(s):  
High Resolution ◽  
Middle Cerebral Artery ◽  
Cerebral Artery ◽  
Focal Cerebral Ischemia ◽  
Control Level ◽  
Experimental Studies ◽  
Arterial Occlusion ◽  
Oxygen Extraction Fraction ◽  
Mca Occlusion ◽  
Border Zones

Experimental models of focal cerebral ischemia have provided important data on early circulatory and biochemical changes, but typically their correspondence with metabolic and hemodynamic findings in stroke patients has been poor. To fill the gap between experimental studies at early time points and rather late clinical studies, we repeatedly measured CBF, CMRO2, oxygen extraction fraction (OEF), cerebral blood volume (CBV), and CMRglc in six cats before and up to 24 h after permanent middle cerebral artery (MCA) occlusion (MCAO), using the 15O steady state and [18F]fluorodeoxyglucose methods and a high-resolution positron emission tomography (PET) scanner. Likewise, three sham-operated control cats were studied during the same period. Final infarct size was determined on serial histologic sections. In the areas of final glucose metabolic depression that were slightly larger than the histologic infarcts, mean CBF dropped to ∼40% of control values immediately on arterial occlusion. It further decreased to <20% during the course of the experiment. This progressive ischemia was most conspicuous in border zones. CMRO2 fell to a lesser degree (55%), eventually reaching ∼25% of its control level. At early stages, OEF increased mainly in the center of ischemia. With time, areas of increased OEF moved from the center to the periphery of the MCA territory. Concurrently, progressive secondary decreases in OEF in conjunction with further reductions of CBF and CMRO2 indicated the development of central necrosis. The findings are highly suggestive of a dynamic penumbra. In five cats with complete MCA infarcts, CBF decreased and OEF increased in the contralateral hemisphere after 24 h, suggesting whole-brain damage. This effect may be explained by the widespread brain edema found histologically in addition to the nonspecific CBF reductions and OEF elevations observed also in the sham-operated controls after 1 day in the experimental condition. In one cat, cortical OEF increased only transiently. Normal CMRO2 and CMRglc were eventually restored, and the final infarct was small. This study demonstrates that acute regional pathophysiologic changes can be repeatedly assessed by multivariate PET in cats. Viable tissue can be detected up to several hours after MCA occlusion, and the transition of misery-perfused regions into necrosis or preserved tissue can be followed over time. The present results support the concept of a dynamic penumbra, in which for up to 24 h tissue damage spreads progressively from the center to the periphery of ischemia. Sequential high-resolution PET provides insight into the dynamics of regional pathophysiology and may thus further the development of rational therapeutic strategies.

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