Evaluation of Serum Levels Superoxide Dismutase in Women with Polycystic Ovarian Syndrome and Gingivitis

2018 ◽  
Vol 30 (2) ◽  
pp. 29-33
Author(s):  
Omar Husham Ali ◽  
Zainab J. Raheem ◽  
Nada K. Imran
Keyword(s):  
Superoxide Dismutase ◽  
Polycystic Ovarian Syndrome ◽  
Serum Levels ◽  
Ovarian Syndrome

scholarly journals The association between anti-Müllerian hormone and vitamin 25(OH)D serum levels and polycystic ovarian syndrome in adolescent females

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Samantha Simpson ◽  
David B. Seifer ◽  
Veronika Shabanova ◽  
Anna Y. Lynn ◽  
Catherine Howe ◽  
...  
Keyword(s):  
Diagnostic Criteria ◽  
Polycystic Ovarian Syndrome ◽  
Retrospective Chart Review ◽  
Serum Levels ◽  
Adolescent Females ◽  
Adult Women ◽  
Clinical Markers ◽  
Cross Sectional ◽  
Pcos Group ◽  
Ovarian Syndrome

Abstract Background High anti-Müllerian hormone (AMH) levels and 25-hydroxyvitamin D [25(OH)D] deficiency have been associated with polycystic ovarian syndrome (PCOS) in adult women, and implicated in its pathogenesis. Herein we determined if the level of both AMH and 25(OH)D are altered in adolescent females with clinical features of PCOS. Methods This is a cross-sectional study utilizing a retrospective chart review of 128 patients aged 12–20 referred to an academic adolescent gynecology and endocrinology clinic for an evaluation of suspected PCOS. Unadjusted comparisons of AMH and 25(OH)D distributions between subjects with and without PCOS were performed using the Wilcoxon Rank Sum test. Quantile regression was used to compare the median AMH and 25(OH)D between subject groups; adjusting for race, ethnicity, BMI, insurance type, age, and season when bloodwork was performed. Results Seventy-four subjects were classified as having PCOS by meeting ≥2 of the three Rotterdam diagnostic criteria, and 47 subjects met only one Rotterdam diagnostic criteria, and were used as the comparative non-PCOS group. There were statistically significant unadjusted differences in median levels of AMH and 25(OH)D. In the adjusted analyses, median AMH was significantly higher in the PCOS group compared to the non-PCOS group (+ 2.39 ng/mL, 95% CI 0.43, 4.35, p = 0.018); 25(OH)D was significantly lower in the PCOS group (− 9.01 ng/mL, 95% CI -14.49, − 3.53 p = 0.001). In our sample, adolescents in both groups had insufficient 25(OH)D level (22 ng/mL) and elevated BMI (32.2 kg/m2). Conclusions Adolescents with PCOS display high levels of AMH and low 25(OH)D levels. Since traditional clinical markers of PCOS may be physiologic in adolescents, AMH and 25(OH)D may be used as surrogate markers of PCOS risk in adolescents.

scholarly journals An Observational Study on 100 Patients with Polycystic Ovarian Syndrome (PCOS)

2014 ◽  
Vol 4 (3) ◽  
pp. 156-160 ◽  
Author(s):  
Khaleda Khanam ◽  
Mimi Parvin
Keyword(s):  
Observational Study ◽  
Polycystic Ovarian Syndrome ◽  
Laboratory Finding ◽  
Serum Levels ◽  
Polycystic Ovaries ◽  
Androgen Excess ◽  
Military Hospitals ◽  
Clinical Presentations ◽  
Lh Secretion ◽  
Ovarian Syndrome

Background: Women with polycystic ovarian syndrome (PCOS) have chronic anovulation and androgen excess not attributable to another cause. The fundamental pathophysiologic defect is unknown. Defects in LH secretion, LH/FSH ratio, amplitude of LH pulsations have been described; but the prevalence of these defects is not still clearly determined. Objective: To review the variable clinical presentations of polycystic ovarian syndrome. Materials and Methods: This observational study was carried out in Combined Military Hospitals of Jessore, Rangpur and Ghatail during November 2008 to June 2013. One hundred patients attending Gynaecology Outpatient Department (GOPD) having at least two of the following criteria ––hyperandrogenism, chronic oligo- or anovulation and ultrasonographic findings were selected. In all selected women LH and FSH serum levels were determined and LH/FSH ratios were calculated. Body mass index (BMI) was measured and was scored to classify their state of obesity. The collected data were compiled and arranged in tables and were subjected to analysis. Results: Most of the patients (92%) were 20–30 years old. Chief complaint of the patients was infertility, either primary (72%) or secondary (28%). Eighty percent women had menstrual irregularities, 30% were hirsute, 71% cases were overweight and 17% were obese. On pelvic ultrasonogram polycystic ovaries were found in 20% cases and 80% had normal ovaries. Thirty percent patients had LH/FSH ratio between 2.1–2.9, 32% had >3 and it was found normal in 38% of cases. Conclusion: PCOS cannot be diagnosed by a single clinical or laboratory finding. The diagnostic approach should be based largely on history and physical examination. DOI: http://dx.doi.org/10.3329/jemc.v4i3.20944 J Enam Med Col 2014; 4(3): 156-160

P–713 The effect of aging and polycystic ovarian syndrome on the maturation and apoptosis of granulosa cells from women undergoing controlled ovarian hyperstimulation with recombinant hFSH

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
K Papageorgiou ◽  
E Mastora ◽  
E Kesikiadou ◽  
A Zisiadi ◽  
A Genopoulou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Granulosa Cells ◽  
Polycystic Ovarian Syndrome ◽  
Oocyte Retrieval ◽  
Serum Levels ◽  
Ongoing Study ◽  
Biological Aging ◽  
Group A ◽  
Group B ◽  
Ovarian Syndrome

Abstract Study question What is the effect of biological aging and polycystic ovarian syndrome (PCOS) on steroidogenic and apoptotic pathways of granulosa cells isolated from women undergoing IVF? Summary answer This analysis revealed that aging as well as PCOS influence the response of women to ovarian stimulation, affecting steroidogenesis and apoptosis on granulosa cells. What is known already Infertility and subfertility affect a significant proportion of humans worldwide. Age-related factors in women lead to a decrease in the ovarian oocyte reserve, and PCOS, a pathological condition characterized by anovulation and hyperandrogenism, are the two most common reasons of subfertility. Despite the progress in recent years, the causes are still ill-defined, and there is a strong need for interventions to ensure good quality oocytes in healthy aging, as well as in PCOS women. Gene expression analyses of granulosa cells isolated during oocyte retrieval are highly informative for understanding the communication between oocytes and follicular cells and optimizing IVF outcomes. Study design, size, duration This report is part of an ongoing study starting from June 2020. 42 women undergoing IVF/ICSI at the University Hospital of Ioannina, Greece, volunteered to participate with informed consent. Participants were divided into three groups: 30- to 35-year-old patients with PCOS, 30- to 35-year-old non-PCOS women, and older women aged 38- to 42-year-old. All participants received 150 – 300IU/d recombinant hFSH. Participants/materials, setting, methods: Granulosa cells (GC) were collected from the follicular fluid at the day of oocyte retrieval using the protocol described by Ferrero et al., 2012 with modifications that allow us to isolate nearly pure populations, also from small or very few follicles. GC purity was determined by FACS and immunocytochemistry. Reverse Transcription-quantitative-PCR was employed to correlate the expression of steroidogenesis- and apoptosis-related genes with the clinical characteristics of each participant, including FSH and progesterone/estradiol serum levels. Main results and the role of chance FACS and immucytochemistry analysis using specific markers of granulosa cells, like FSHR, as well as leucocyte and erythrocyte cell markers (CD45 and Glycophorin) confirmed the purity of the isolated granulosa cell populations. No differences were found in hormone serum levels between 30- to 35-year-old PCOS (Group A) and non-PCOS women of the same age (Group B) whereas progesterone and estradiol serum levels were lower in 38- to 42-year-old women (Group C) compared to group B. RT-qPCR analysis of maturation-related genes (ZP3 and AHR) revealed the presence of more immature follicles in Group A compared to the other groups. The granulosa cells from women of group A were characterized by increased expression of genes involved in steroidogenic pathways (such as FSHR, CYP17A1) compared to group B, as well as of the genes for the estradiol and progesterone receptors (ESR1 and PGRMC1, respectively), and by decreased levels of the apoptosis-related genes BAX and BCL-xL. Importantly, the granulosa cells from the women of Group C were characterized by lower mRNA levels of steroidogenesis-related genes, as well as of ESR1, and by increased apoptosis as revealed by the increased ratio of BAX/BCL2 transcripts, compared to Group A. Limitations, reasons for caution The presented results of gene expression analysis are based on preliminary data as the number of participants is small (6 women per group), but they are part of an ongoing study where the number of participants is increasing in order to improve quantification and reproducibility of our results. Wider implications of the findings: Potential differences in signaling pathways that are crucial for the maturation of granulosa cells and follicles during biological aging or in pathological conditions such as PCOS are expected to provide valuable information concerning the response of individual women to gonadotropin stimulation and might help to design more personalized therapeutic strategies. Trial registration number Not applicable

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