SARS-CoV-2 Omicron Neutralization After Heterologous Vaccine Boosting

As part of an ongoing study assessing homologous and heterologous booster vaccines, following primary EUA series, we assessed neutralization of D614G and Omicron variants prior to and 28 days after boost. Subset analysis was done in six combinations (N = 10/group): four homologous primary-booster combinations included mRNA-1273 two-dose priming followed by boosting with 100-μg or 50-μg mRNA-1273, Ad26.COV2.S single-dose priming followed by Ad26.COV2.S booster and BNT162b2 two-dose priming followed by BNT162b2 boosting; and two heterologous primary-booster combinations: BNT162b2 followed by Ad26.COV2.S and Ad26.COV2.S followed by BNT162b2. Neutralizing antibody (Nab) titers to D614G on the day of boost (baseline) were detected in 85-100% of participants, with geometric mean titers (GMT) of 71-343 in participants who received an mRNA vaccine series versus GMTs of 35-41 in participants primed with Ad26.OV2.S. Baseline NAb titers to Omicron were detected in 50-90% of participants who received an mRNA vaccine series (GMT range 12.8-24.5) versus 20-25% among participants primed with Ad26.COV2.S. The booster dose increased the neutralizing GMT in most combinations to above 1000 for D614G and above 250 for Omicron by Day 29. Homologous prime-boost Ad26.COV2.S had the lowest NAb on Day 29 (D614G GMT 128 and Omicron GMT 45). Results were similar between age groups. Most homologous and heterologous boost combinations examined will increase humoral immunity to the Omicron variant.

Suffixation and sequentiality

This paper offers some reflections on the study of morphology – broadly speaking, ‘word formation’ – as a participants’ resource in social interaction. I begin by calling attention to morphology as a comparatively underexamined component of linguistic structure by conversation analysts and interactional linguists, in that it has yet to receive the same dedicated consideration as have, e.g., phonetics and syntax. I then present an ongoing study of suffixes/suffixation in Spanish – focusing on diminutives (e.g., –ito), augmentatives (e.g., –ote), and superlatives (i.e., –ísimo) – and describe how the sequentiality of interaction can offer analysts profound insight into participants’ orientations to morphological resources. With what I refer to as ‘morphological transformations’ – exemplified here in both same-turn and next-turn positions – interactants sequentially construct and expose morphological complexity as such, locally instantiating its relevance in the service of action. It is argued that a focus on transformations therefore provides analysts with a means to ‘break into’ morphology-based collections. A range of cases are presented to illustrate this methodological approach, before a concluding discussion in which I describe how morphology-focused investigations may intersect with explorations of other interactional phenomena.

Manual Didática da Aritmética e os Saberes a e para ensinar sistematizados por Ismael França Campos (1950-1960)

O presente artigo é o extrato de um estudo em curso, no âmbito da Iniciativa Ciência, que tem por objetivo caracterizar elementos dos saberes a e para ensinar sistematizados para a formação de professores sobre o conteúdo de aritmética, especificamente no ensino de frações em tempos de orientações da psicologia científica elaboradas durante o movimento Escola Nova sobre o Manual Didática da Aritmética. Cortesia de Ismael França Campos, Diretor e Professor do Instituto de Educação (IE) (1943-1964). Pela abordagem teórico-metodológica, a pesquisa assume a forma de um estudo sócio-histórico. Na análise, os saberes que sustentam o movimento da Escola Nova relacionam-se com os métodos de ensino e neste Manual há orientações direcionadas aos professores de como ensinar. Os resultados encontrados mostram que os saberes a e para ensinar no material analisado estão em diálogo com as ideias da psicologia científica.Didactic Manual of Arithmetic and Knowledge for and to teaching systematized by Ismael França Campos (1950 -1960)This article is an extract from an ongoing study, within the scope of the Science Initiative, which aims to characterize elements of knowledge to and for teach systematized for the training of teachers on the content of arithmetic, specifically in the teaching of fractions in times of orientation of scientific psychology, developed during the Escola Nova movement on the Didactic Manual of Arithmetic. Courtesy of Ismael França Campos, Director and Professor at the Instituto de Educação (IE) (1943-1964). Through the theoretical-methodological approach, the research takes the form of a socio-historical study. In the analysis, the knowledge that sustains the Escola Nova movement is related to the teaching methods and in this Manual, there are guidelines directed to teachers on how to teach. The results found show. The results found show that the knowledge for and to teach in the analyzed material is in dialogue with the ideas of scientific psychology.Keywords: Ismael França Campos; History of mathematics education; Teaching profession; Arithmetic.

Social Interaction Style in Autism: An Inquiry into Phenomenological Methodology

Autistic difficulties with social interaction have primarily been understood as expressions of underlying impairment of the ability to ‘mindread.’ Although this understanding of autism and social interaction has raised controversy in the phenomenological community for decades, the phenomenological criticism remains largely on a philosophical level. This article helps fill this gap by discussing how phenomenology can contribute to empirical methodologies for studying social interaction in autism. By drawing on the phenomenology of Maurice Merleau-Ponty and qualitative data from an ongoing study on social interaction in autism, I discuss how qualitative interviews and participant observation can yield phenomenologically salient data on social interaction. Both, I argue, enjoy their phenomenological promise through facilitating attention to the social-spatial-material fields in and through which social interactions and experiences arise. By developing phenomenologically sound approaches to studying social interaction, this article helps resolve the deficiency of knowledge concerning experiential dimensions of social interaction in autism.

Description of the Process to Validate the Mexican Nurse Informatics Competency Self-Assessment Scale

Nurses need to have sufficient competencies in nursing informatics to be able to provide safe and efficient care. The Self-Assessment of Nursing Informatics Competencies Scale (SANICS) has been developed and validated as a self-report measure of informatics competencies in Western settings. In this work, we describe the ongoing study that aims to validate and translate SANICS into the Mexican setting.

Prince Boun Oum and the March 24, 1945 Treaty: A Possibility of Independent Champassak Following World War II

This article is part of the author’s ongoing study of Champassak and the formation of the new Lao state, with the aim of understanding Champassak’s dubious standing within Lao state, Siam, French Indochina, and even its self-image. In terms of historical methodology, the author discusses the treaty reached between Prince Boun Oum and Charles de Gaulle’s provisional government on March 24, 1945, which promised independence and membership in the French Union after WWII. Although Prince Boun Oum’s ambitious goal of independence for Champassak failed, this situation could be viewed as another form of the Lao state that was overlooked after Lao independence. Finally, the Champassak case is relevant to the debate about the current existence of a nation-state in this region and ethnic nationalism.

Well Done Is Better than Well Said

Heralding Benjamin Franklin as a model for diversity, equity, inclusion, and accessibility (DEIA) can be viewed as peculiar as it sounds. Without question, Franklin's personal and professional histories as a past slaveholder, alleged womanizer, and bully are problematic. Yet, the messiness of Franklin's life with all of its inherent complications and his transformation into a champion for the oppressed makes him a ripe subject for ongoing study. Moreover, Franklin's life example can help us formulate a roadmap for how we too can evolve and transform in our thinking, allyship, and approaches to diversity, equity, inclusion, and accessibility.

Pharmacogenomics of Fludarabine in Pediatric HCT Recipients: Preliminary Results

Introduction: Total Body Irradiation (TBI) conditioning regimens are considered standard of care for pediatric hematopoietic cell transplant (HCT) recipients. However, TBI has many long-term effects, so avoiding it in young HCT recipients is critical. Fludarabine is frequently used in combination with other chemotherapeutic agents like busulfan and/or thiotepa in non-TBI based conditioning regimens. While multi-drug conditioning aims to avoid TBI-related late-effects, little is known about the effect of variations in genes that metabolize these drugs (pharmacogenomics/PGx) on clinical outcomes including engraftment, relapse, and acute drug-related toxicities. In this study, we investigated the pharmacogenomic effects on HCT outcomes such as event-free outcomes and re-transplantation outcomes, as well as fludarabine's pharmacokinetics (PK). Methods: This ongoing study included pediatric patients from a nationwide multi-center study who received fludarabine in combination with other agents as busulfan and thiotepa. From literature searches and PharmGKB databases, we selected 70 SNPs in 30 candidate pharmacological genes for these drug pathways. SNPs with a minor allele frequency of 10% were further selected for analysis with different endpoints such as: 1) Cumulative incidence with disease relapse with re-transplantation (using Gray's methods, controlling for competing risks); 2) fludarabine PK with cumulative area under the curve (cAUC); 3) 1-month-post-transplant chimerism levels defined as sufficient (CD3 ≥80% and CD14/15 ≥95% chimerism) and insufficient (CD3 <80% and CD14/15 <95% chimerism); and 4) event-free outcomes (comparing patients alive and well vs. others) OR alive re-transplantation outcomes (alive with vs. without re-transplantation). SNPs with an association p-value <0.05 were considered significant using generalized linear models from the SNPassoc R package. Results: Total of 87 patients included in the analysis had a median age of 3.5 (0.2 - 17.9) years, 63% were male, 48% were Caucasian, 85% had received allogenic HCT, and 43% had malignant hematologic disease (Table 1). For SNP rs2277119 in CYP39A1, presence of T allele was associated with greater cumulative incidence of re-transplantation (TC-TT vs. CC genotype: HR=3.64 (95% CI=1.14-11.62), p=0.02, Figure 1A); and SNP rs4715354 in GSTA5, presence of G allele was associated with lower cumulative incidence of re-transplantation (AG-GG vs. AA: HR=0.32 (95% CI=0.11-0.95), p=0.037, Figure 1B). In 79% of patients with available fludarabine cAUC (median of 3.72 mg•hr/L), we evaluated fludarabine pathway gene SNPs for association with cAUC. Within SNPs rs3925058 in CMPK1 and rs11853372 in the uptake transporter SLC28A1, presence of variant allele was associated with higher fludarabine cAUC (rs3925058: GA-AA vs. GG, p=0.049, Figure 2A, and rs11853372 GG-GT vs. TT, p=0.003, Figure 2B); and for a 3'-UTR SNP rs2037067 in TENM3/DCTD, the variant allele was associated with lower fludarabine cAUC levels (CT-CC vs. TT, p=0.005, Figure 2C). In an exploratory analysis with event-free outcomes, patients with variant allele for SNPs rs9937 in RRM1, rs2072671 in CDA, rs324148 in SLC29A1, and rs7533657 in CTPS1 had higher odds of having an event compared to patients with the reference allele; while rs11577910 in CTPS1 had a better outcome with presence of variant allele. With respect to patient's status being alive with re-transplantation outcomes, variants SNPs with rs507964 in SLC29A1, rs4244285 in CYP2C19, rs1021737 in CTH, rs1561876 in STIM1, and rs1130609 in RRM2 have high odds of re-transplantation. At 1-month follow-up, variant SNP rs12144160 in CTPS1 was associated with sufficient chimerism in CD3 and CD14/15. Conclusions: In this study, we have identified a number of SNPs that predict interpatient variability in clinical outcomes and fludarabine levels in pediatric HCT recipients. Results so far showed potential to predict outcomes and develop strategies that will consider pharmacogenomics when determining fludarabine doses in pediatric HCT recipients. Our ongoing study is focused on establishing and validating the pharmacogenetic markers predictive of pharmacokinetics of busulfan, fludarabine and thiotepa and clinical outcomes in pediatric HCT recipients. Acknowledgements: This study was supported by Florida Department of Health - Live Like Bella Discovery Award (9LA04). Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.

AIM against survey fraud

Objectives Although there exists a variety of anonymous survey software, this study aimed to develop an improved system that incentivizes responses and proactively detects fraud attempts while maintaining anonymity. Materials and Methods The Anonymous Incentive Method (AIM) was designed to utilize a Secure Hash Algorithm, which deterministically assigned anonymous identifiers to respondents. An anonymous raffle system was established to randomly select participants for a reward. Since the system provided participants with their unique identifiers and passwords upon survey completion, participants were able to return to the survey website, input their passwords, and receive their rewards at a later date. As a case study, the validity of this novel approach was assessed in an ongoing study on vaping in high school friendship networks. Results AIM successfully assigned irreversible, deterministic identifiers to survey respondents. Additionally, the particular case study used to assess the efficacy of AIM verified the deterministic aspect of the identifiers. Discussion Potential limitations, such as scammers changing the entry used to create the identifier, are acknowledged and given practical mitigation protocols. Although AIM exhibits particular usefulness for network studies, it is compatible with a wide range of applications to help preempt survey fraud and expedite study approval. Conclusion The improvements introduced by AIM are 2-fold: (1) duplicate responses can be filtered out while maintaining anonymity and (2) the requirement for the participant to keep their identifier and password for some time before returning to the survey website to claim a reward ensures that rewards only go to actual respondents.