scholarly journals Paper review: An overview on microarray technologies

2021 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Rohmatul Fajriyah
Keyword(s):  
Tissue Sample ◽  
Life Sciences ◽  
Microarray Experiment ◽  
Affymetrix Genechip ◽  
Microarray Technology ◽  
Biotechnology Research ◽  
Paper Review

Bioinformatics is a branch in Statistics which is still unpopular among statistics students in Indonesia. Bioinformatics research used microarray technology, because data is available through to microarray experiment on tissue sample at hand. Microarray technology has been widely used to provide data for bioinformatics research, since it was first introduced in late 1990, particularly in life sciences and biotechnology research. The emergence and development of the Covid-19 disease further reinforces the need to understand bioinformatics and its technology. There are two of the most advance platforms in microarray technology, namely, are the Affymetrix GeneChip and Illumina BeadArray.  This paper aims to give an overview about microarray technology on the two platforms and the advantage of using them on bioinformatics research.

Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling

2002 ◽  
Vol 69 ◽  
pp. 135-142 ◽  
Author(s):  
Elena M. Comelli ◽  
Margarida Amado ◽  
Steven R. Head ◽  
James C. Paulson
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Affymetrix Genechip ◽  
Microarray Technology ◽  
Gene Arrays ◽  
Glycosyltransferase Gene

The development of microarray technology offers the unprecedented possibility of studying the expression of thousands of genes in one experiment. Its exploitation in the glycobiology field will eventually allow the parallel investigation of the expression of many glycosyltransferases, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis. While numerous gene arrays are available on the market, e.g. the Affymetrix GeneChip® arrays, glycosyltransferases are not adequately represented, which makes comprehensive surveys of their gene expression difficult. This chapter describes the main issues related to the establishment of a custom glycogenes array.

Microfluidics and Beyond – Devices for Applications in Biotechnology -

2004 ◽  
Vol 820 ◽  
Author(s):  
Martina Daub ◽  
Rolf M. Kaack ◽  
Oliver Gutmann ◽  
Chris P. Steinert ◽  
Remigius Niekrawietz ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Life Science ◽  
Life Sciences ◽  
Microarray Technology ◽  
Parallel Operation ◽  
Modern Life ◽  
Liquid Handling ◽  
Microtiter Plates ◽  
Integration Density ◽  
Volume Range

AbstractFor the performance of certain analytical and diagnostic tasks in modern Life Science applications high throughput screening (HTS) methods are essential. Miniaturization, parallelization and automation allow to decrease consumption of expensive materials and lead to faster analyzing times. The miniaturization of total assay volumes by the use of microtiter plates as well as the microarray technology have revolutionized the field of biotechnology and Life Sciences. Neither printing of microarrays with droplet volumes of several picoliters, nor handling of precious enzymes in the upper nanoliter range can be accomplished with traditional liquid handling devices like air displacement pipettes. The development of novel low volume liquid handling devices, which are subject to current research, addresses the diverse requirements shifting steadily to lower volumes. Various novel non-contact dispensing methods in the nanoliter and picoliter range are presented and classified according to their working principles like air displacement and direct displacement methods (TopSpot®, NanoJetTM, Dispensing Well PlateTM). Properties of the various methods are compared in terms of flexibility, integration density, speed of operation, precision, addressable volume range and amenability to multi-parallel operation.

scholarly journals GENE EXPRESSION STUDIES USING MICROARRAYS: PRINCIPLES, PROBLEMS, AND PROSPECTS

2002 ◽  
Vol 26 (4) ◽  
pp. 256-270 ◽  
Author(s):  
David Murphy
Keyword(s):  
Gene Networks ◽  
Time Course ◽  
Expression Patterns ◽  
Rna Isolation ◽  
Microarray Experiment ◽  
Bioinformatic Analysis ◽  
Microarray Technology ◽  
Microarray Probe ◽  
Advantages And Disadvantages ◽  
Gene Expression Studies

Anumber of mammalian genomes having been sequenced, an important next step is to catalog the expression patterns of all transcription units in health and disease by use of microarrays. Such discovery programs are crucial to our understanding of the gene networks that control developmental, physiological, and pathological processes. However, despite the excitement, the full promise of microarray technology has yet to be realized, as the superficial simplicity of the concept belies considerable problems. Microarray technology is very new; methodologies are still evolving, common standards have yet to be established, and many problems with experimental design and variability have still to be fully understood and overcome. This review will describe the time course of a microarray experiment—RNA isolation from sample, target preparation, hybridization to the microarray probe, data capture, and bioinformatic analysis. For each stage, the advantages and disadvantages of competing techniques are compared, and inherent sources of error are identified and discussed.

Feature

2011 ◽  
Vol 15 (03) ◽  
pp. 12-62 ◽  
Keyword(s):  
Structural Biology ◽  
Open Innovation ◽  
Life Sciences ◽  
Future Directions ◽  
Biotechnology Research

CROs: Challenges to Contiuing Growth. Life Sciences Capital Investing and Bioentrepreneurship in China and Asia Taking Off. Flexing the Muscles of BioVentures in Japan and Beyond. Future Directions and Growth of Biotechnology in China: An Analysis. Potential Abounds, Biotechnology Research in China. Biotechnology Parks: China into the Next Future. Interview with Kurt Wüthrich – Why Structural Biology Matters. The PCR Revolution – An Interview with Carl T Wittwer. Vietnam Healthcare – The Next Growth Frontier? Open Innovation: Next Frontier In Global Biopharma Industry.

scholarly journals Best Practices for Artificial Intelligence in Life Sciences Research

2020 ◽  
Author(s):  
Vladimir Makarov ◽  
Terry Stouch ◽  
Brandon Allgood ◽  
Christopher Willis ◽  
Nick Lynch
Keyword(s):  
Artificial Intelligence ◽  
Machine Learning ◽  
Best Practices ◽  
Life Sciences ◽  
Organizational Management ◽  
Biotechnology Research

We describe 11 best practices for the successful use of Artificial Intelligence and Machine Learning in the pharmaceutical and biotechnology research, on the data, technology, and organizational management levels.

Biomedical applications: Pitfalls in the practice

1994 ◽  
Vol 52 ◽  
pp. 378-379
Author(s):  
J. D. Shelburne ◽  
Peter Ingram ◽  
Victor L. Roggli ◽  
Ann LeFurgey
Keyword(s):  
Operating Room ◽  
Liquid Nitrogen ◽  
Lung Tissue ◽  
Biomedical Applications ◽  
Microprobe Analysis ◽  
Tissue Sample ◽  
Cellular Level ◽  
Tissue Samples ◽  
Asbestos Fibers

At present most medical microprobe analysis is conducted on insoluble particulates such as asbestos fibers in lung tissue. Cryotechniques are not necessary for this type of specimen. Insoluble particulates can be processed conventionally. Nevertheless, it is important to emphasize that conventional processing is unacceptable for specimens in which electrolyte distributions in tissues are sought. It is necessary to flash-freeze in order to preserve the integrity of electrolyte distributions at the subcellular and cellular level. Ideally, biopsies should be flash-frozen in the operating room rather than being frozen several minutes later in a histology laboratory. Electrolytes will move during such a long delay. While flammable cryogens such as propane obviously cannot be used in an operating room, liquid nitrogen-cooled slam-freezing devices or guns may be permitted, and are the best way to achieve an artifact-free, accurate tissue sample which truly reflects the in vivo state. Unfortunately, the importance of cryofixation is often not understood. Investigators bring tissue samples fixed in glutaraldehyde to a microprobe laboratory with a request for microprobe analysis for electrolytes.

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